Identification and expression analysis of cytochrome P450 genes in Plecoptera oculata (Lepidoptera: Noctuidae)

Zi-Hao LYU,Delong KONG,Jie CHENG,Tong Lin 한국곤충학회 2020 Entomological Research Vol.50 No.2

Cytochrome P450 monooxygenases (CYPs) play an important role in the decomposition of insecticides. Plecoptera oculata Moore is a lepidopteran forestry pest that severely affects Dalbergia odorifera T. Chen. In this study, 27 putative CYP genes were identified in the transcriptome of larval P. oculata. Phylogenetic analysis showed that these genes are grouped into four clans, 15 families, and 22 subfamilies. The tissue-specific expression profile for each gene was determined using real-time reverse transcription-quantitative PCR (RT-qPCR), and the 27 genes were found to be ubiquitously expressed, mainly in the midgut, Malpighian tubules, and fat body. The expression profiles of all 27 PoCYP genes exposed to half the lethal concentration (LC50) of sendebao and lambda-cyhalothrin were determined using RT-qPCR. The results showed that six and twelve PoCYP genes were significantly down- and up-regulated, respectively, by exposure to sendebao, and three and twelve PoCYP genes were significantly down- and up-regulated, respectively, by exposure to lambda-cyhalothrin. To the best of our knowledge, this study is the first to show the expression of CYP genes in P. oculata. Our results provide a basis for further investigation of the roles of PoCYP genes in insecticide detoxification and as potential targets for novel pesticides.

Lytic Bacteriophage as a Biomaterial to Prevent Biofilm Formation and Promote Neural Growth

Liu Zi-Hao,Chiang Ming-Tse,Lin Hsin-Yi 한국조직공학과 재생의학회 2022 조직공학과 재생의학 Vol.19 No.5

BACKGROUND Although non-lytic filamentous bacteriophages have been made into biomaterial to guide tissue growth, they had limited ability to prevent bacterial infection. In this work a lytic bacteriophage was used to make an antibacterial biomaterial for neural tissue repair. METHODS Lytic phages were chemically bound to the surface of a chitosan film through glutaraldehyde crosslinking. After the chemical reaction, the contact angle of the sample surface and the remaining lytic potential of the phages were measured. The numbers of bacteria on the samples were measured and examined under scanning electron microscopy. Transmission electron microscopy (TEM) was used to observe the phages and phage-infected bacteria. A neuroblast cell line was cultured on the samples to evaluate the sample’s biocompatibility. RESULTS The phages conjugated to the chitosan film preserved their lytic potential and reduced 68% of bacterial growth on the sample surface at 120 min (p\0.001). The phage-linked surface had a significantly higher contact angle than that of the control chitosan (p\0.05). After 120 min a bacterial biofilm appeared on the control chitosan, while the phagelinked sample effectively prevented biofilm formation. The TEM images demonstrated that the phage attached and lysed the bacteria on the phage-linked sample at 120 min. The phage-linked sample significantly promoted the neuroblast cell attachment (p\0.05) and proliferation (p\0.01). The neuroblast on the phage-linked sample demonstrated more cell extensions after day 1. CONCLUSION The purified lytic phages were proven to be a highly bioactive nanomaterial. The phage-chitosan composite material not only promoted neural cell proliferation but also effectively prevent bacterial growth, a major cause of implant failure and removal.

A Novel Battery State of Health Estimation Method Based on Outlier Detection Algorithm

Chang-hao Piao,Zi-hao Hu,Ling Su,Jian-fei Zhao 대한전기학회 2016 Journal of Electrical Engineering & Technology Vol.11 No.6

A novel battery SOH estimation algorithm based on outlier detection has been presented. The Battery state of health (SOH) is one of the most important parameters that describes the usability state of the power battery system. Firstly, a battery system model with lifetime fading characteristic was established, and the battery characteristic parameters were acquired from the lifetime fading process. Then, the outlier detection method based on angular distribution was used to identify the outliers among the battery behaviors. Lastly, the functional relationship between battery SOH and the outlier distribution was obtained by polynomial fitting method. The experimental results show that the algorithm can identify the outliers accurately, and the absolute error between the SOH estimation value and true value is less than 3%.

A Novel Battery State of Health Estimation Method Based on Outlier Detection Algorithm

Piao, Chang-hao,Hu, Zi-hao,Su, Ling,Zhao, Jian-fei The Korean Institute of Electrical Engineers 2016 Journal of Electrical Engineering & Technology Vol.11 No.6

A novel battery SOH estimation algorithm based on outlier detection has been presented. The Battery state of health (SOH) is one of the most important parameters that describes the usability state of the power battery system. Firstly, a battery system model with lifetime fading characteristic was established, and the battery characteristic parameters were acquired from the lifetime fading process. Then, the outlier detection method based on angular distribution was used to identify the outliers among the battery behaviors. Lastly, the functional relationship between battery SOH and the outlier distribution was obtained by polynomial fitting method. The experimental results show that the algorithm can identify the outliers accurately, and the absolute error between the SOH estimation value and true value is less than 3%.

The Impact of the Competitiveness of Intermediate Software on Enterprise Results

Zi-Yang Liu,Yi Chen,Hao Li 한국컴퓨터정보학회 2018 한국컴퓨터정보학회 학술발표논문집 Vol.26 No.2

The purpose of this paper is to draw a conclusion on the impact of intermediate software on enterprise results. In this paper, product innovation and product reliability are especially used as analytical factors. An exploratory analytical study is conducted on the competitiveness of intermediate software, in the hope of gaining a new understanding of the competitiveness of intermediate software. Data are analyzed using such quantitative analytical tools as SPSS and AMOS. Using reliability analysis, validity analysis and structural equation model analysis, the final results are achieved. According to the analysis results, we can draw the following conclusions: the competitiveness of intermediate software has a positive impact on the innovation of software products. The competitiveness of intermediate software doesn’t have a positive impact on the reliability of software products. Product innovation has a positive impact on enterprise results. Product reliability also has a positive impact on enterprise results. By analyzing the conclusions, we can make certain suggestions and draw implications on the competitiveness of China’s software industry.

Application of harmonic radar to analyze dispersal behavior of the Japanese pine sawyer beetle, Monochamus alternatus (Coleoptera Cerambycidae)

Zi-Yi ZHANG,Yu-Ping ZHA,San-ShanCAI,Cheng-Hao HONG,Peng Liang,Jing-Yuan CHEN 한국곤충학회 2020 Entomological Research Vol.50 No.1

Monochamus alternatus (Hope) is a severe wood-boring pest in coniferous forests and a major vector of pine wilt disease in East Asia. Harmonic radar is a powerful tool for studying the dispersal behavior of insects and it could be applied to control pine wilt disease. In this study, we validated the application of harmonic radar for analyzing the dispersal behavior of M. alternatus beetles in a field environment. We determined the wing capacities of the beetles and the effects of electronic tagging and marking on their movement, flight ability, survivorship, and food consumption in the laboratory to confirm the suitability of this technique. The detection rate and recovery rate for beetles were analyzed separately using radar on caged pine stands and in the field environment. The results showed that the minimum wing capacity of the Japanese pine sawyer was 24.9 mg, which was seven times the weight of the electronic tag (3.5 mg). Marking and tagging the beetles had no significant adverse effects on their movement, flight capacity, food consumption, and survivorship. The detection rate using the radar system and recovery rate based on visual observations of the beetles in caged pines were both 95.6%. However, in the field environment, the detection and recovery rates were only 55.6% and 37.8% after one week, respectively, and 33.3% and 7.8% after two weeks. Harmonic radar is a promising technique for studying the dispersal behavior of the Japanese pine sawyer, but its performance is not satisfactory and major improvements are required for both the radar system and electronic tags.

Secondary Metabolites with Anti-complementary Activity from the Stem Barks of Juglans mandshurica Maxim

( Zi-jiang Li ),( Shilin Chen ),( Xiang-hao Yang ),( Rui Wang ),( Hee-jeong Min ),( Lei Wu ),( Chuan-ling Si ),( Young-soo Bae ) 한국목재공학회 2018 목재공학 Vol.46 No.2

Juglans mandshurica is a fast growing hard species, which is a tree in family of Juglandaceae and has a wide distribution in China, Korea and eastern Russia. Plant materials from J. mandshurica have extensively been used in folk medicines to prevent or cure gastric, esophageal, lung and cardiac cancer. As one chain of our searching for anticomplementary agents from natural sources, two epimeric ellagitannins, [2,3-O-4,4′,5,5′,6,6′,-hexahydroxydiphenoyl (HHDP))-(α,β)-D-glucose] (I) and pedunculagin (II) were purified from 70% acetone extracts of the stem barks of J. mandshurica by Thin Layer Chromatography and Sephadex LH-20 column chromatography approaches. The chemical structures of the isolated compounds were characterized by MS, NMR, and a careful comparation with published literatures. The epimeric ellagitannins I and II exhibited inhibitory properties against a classical pathway of complementary system with 50 % inhibitory concentrations (IC₅₀) values of 65.3 and 47.7 μM, respectively, comparing with riliroside (IC₅₀=104 μM) and rosmarinic acid (IC₅₀=182 μM), which were used as positive controls. Thus, the work indicated both the two secondary metabolites possess excellent inhibitory activity and might be developed as potential anticomplementary chemicals.

Research on Use Motivations of Mobile Payment Adopters

Zi-Yang Liu(유자양),Hao Li(이호),Yi Chen(진예) 한국컴퓨터정보학회 2018 韓國컴퓨터情報學會論文誌 Vol.23 No.8

In this paper we purposes to analyze the motivation of mobile payment adopters. In this study, with unified theory of acceptance and use of technology (UTAUT) model as the basis of research, we set up a structural equation model based on gender, age, experience and voluntary, collected data in the form of a questionnaire survey and analyzed drivers that affected mobile payers. In this study, The necessity of this paper is that the group of Korean students belongs to a special group with nearly 70,000 people. The penetration rate of mobile payment in China is 77%, ranking first in the world, but after the young people came to Korea. There are many obstacles and inconveniences when using mobile payment. We have proposed special subjects for special populations. the survey data were analyzed using SPSS22.0 and AMOS22.0 statistical software and the proposed model was modified. The results show that consumers’ mobile payment is mainly motivated by social influence and performance expectancy. These results are of theoretical value and offer a new direction and implications to mobile payment developers. Besides, they also provide useful information and guidance for enterprises to aim at the market of Chinese oversea students in South Korea.

Development of Nutrient Solution for Chenopodiaceae Leaf Vegetables Based on Nutrient-Water Absorption Rate and Cation Ratio

Hua Zi Huang,Cheng Hao Zhang,Hyung Kweon Yoon,Tae Cheol Seo 한국원예학회 2007 Horticulture, Environment, and Biotechnology Vol.48 No.1

Nutrient solution for various Chenopodiaceae leaf vegetables was developed. Various strength (1/2, 1, and 3/2) of nutrient solution recommended by National Horticultural Research Institute, Korea was applied to the crops in deep flow technique system for 27 days. Growth of ‘Atras’ spinach, ‘Detroit Dark’ beet, and ‘Swiss’ red chard was highest in the treatment of 3/2 strength. The proper constitution of nutrient solution for the Chenopodiaceae leaf vegetables was 11 N, 2.5 P, 8 K, 2 Ca, and 2 me/L Mg. The crops were grown two times in March and September to examine the appropriateness of the nutrient solution. Relative growth rate and the amount of ascorbic acid treated with the nutrient solution were increased by 1.1-1.4 and 1.1-1.2 times, respectively, and the amount of chlorophyll was increased by 1.0-1.2 times.

Effect of Sodium-Selenate Application on the Growth and Absorption Ability of Garlic Grown in the Open Field

Chang Hao Zhang,Hyung Kwon Yun,Tae Cheol Seo,Hua Zi Huang,Eun Young Yang 한국원예학회 2006 한국원예학회 기타간행물 Vol.- No.-