NEDD4L suppresses PD-L1 expression and enhances anti-tumor immune response in A549 cells

Zhong Bin,Zheng Jie,Wen Huilan,Liao Xinhui,Chen Xingxiang,Rao Yunwei,Yuan Ping 한국유전학회 2022 Genes & Genomics Vol.44 No.9

Background: Non-small cell lung cancer (NSCLC) poses a salient threat to public health. E3 ubiquitin ligase commonly functions as an anti-tumor role. Objective: This study probed the effect of E3 ligase NEDD4L on A549 cells. Methods: NEDD4L expression in NSCLC and its correlation with NSCLC patient's prognosis were predicted and verified. PD-L1 protein level was measured, and the correlation between NEDD4L and PD-L1 was analyzed. The effects of NEDD4L overexpression on the binding of NEDD4L to PD-L1 and ubiquitination level of PD-L1 were examined. Xenograft tumor model was established in mice. The volume and weight of xenograft tumors were recorded. The proportion of CD8+ T cells and contents of IL-2 and INF-γ were detected. Results: NEDD4L expression was downregulated in NSCLC tissues and A549 cells, and correlated with poor prognosis of NSCLC patients. PD-L1 was upregulated in NSCLC and negatively correlated with NEDD4L. Overexpression of NEDD4L upregulated ubiquitination level of PD-L1 and reduced protein level of PD-L1. Overexpression of NEDD4L decreased tumor volume and weight and enhanced proportion of CD8+ T cells and contents of IL-2 and INF-γ. Conclusions: Collectively, overexpression of NEDD4L suppressed PD-L1 protein level through ubiquitination, thereby enhancing anti-tumor immune response and retarding NSCLC progression.

Power Allocation for Opportunistic Full-Duplex based Relay Selection in Cooperative Systems

( Bin Zhong ),( Dandan Zhang ),( Zhongshan Zhang ) 한국인터넷정보학회 2015 KSII Transactions on Internet and Information Syst Vol.9 No.10

In this paper, performance analysis of full-duplex (FD) relay selection under decode-and-forward (DF) relaying mode is carried out by taking into account several critical factors, including the distributions of the received signal-to-noise ratio (SNR) and the outage probability of wireless links. The tradeoff between the FD and half-duplex (HD) modes for relay selection techniques is also analyzed, where the former suffers from the impact of residual self-interference, but the latter requires more channel resources than the former (i.e., two orthogonal channels are required). Furthermore, the impact of optimal power allocation (OPA) on the proposed relay-selection scheme is analyzed. Particularly, the exact closed-form expressions for outage probability of the proposed scheme over Rayleigh fading channels are derived, followed by validating the proposed analysis using simulation. Numerical results show that the proposed FD based scheme outperforms the HD based scheme by more than 4 dB in terms of coding gain, provided that the residual self-interference level in the FD mode can be substantially suppressed to the level that is below the noise power.

Data-driven prognostics method for turbofan engine degradation using hybrid deep neural network

Bin Xue,Zhong-bin Xu,Xing Huang,Peng-cheng Nie 대한기계학회 2021 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.35 No.12

Powerful sequence modeling capability for massive multi-sensor data enables deep-learning-based methods to obtain accurate remaining useful life (RUL) estimations. Hybrid neural networks, with learned representations based on various networks, have enhanced the prognostics accuracies than single networks. However, assembly strategies that are limited to either parallel or serial, and insufficient utilization of single networks restrict the development of hybrid networks for more complex problems. This paper proposes a datadriven method using hybrid multi-scale convolutional neural network (MSCNN) and bidirectional long short-term memory (BLSTM) network (namely HMCB network) for RUL estimation. The framework of the network includes two parallel paths. One is composed of MSCNN and BLSTM in serial and the other is a BLSTM path. The HMCB network integrates the merits of multi-scale spatial feature extraction of MSCNN and sequence learning capacity of BLSTM. Validated by C-MAPSS dataset, the HMCB network demonstrates noticeably higher prognostic accuracy than other state-of-the-art methods.

Partial Relay Selection in Decode and Forward Cooperative Cognitive Radio Networks over Rayleigh Fading Channels

( Bin Zhong ),( Zhongshan Zhang ),( Dandan Zhang ),( Keping Long ),( Haiyan Cao ) 한국인터넷정보학회 2014 KSII Transactions on Internet and Information Syst Vol.8 No.11

The performance of an partial relay selection on the decode-and-forward (DF) mode cognitive radio (CR) relay networks is studied, with some important factors, including the outage probability, the bit error ratio (BER), and the average channel capacity being analyzed. Different from the conventional relay selection schemes, the impact of spectrum sensing process as well as the spectrum utilization efficiency of primary users on the performance of DF-based CR relaying networks has been taken into consideration. In particular, the exact closed-form expressions for the figures of merit such as outage probability, BER, and average channel capacity over independent and identically distributed (i.i.d.) Rayleigh fading channels, have been derived in this paper. The validity of the proposed analysis is proven by simulation, which showed that the numerical results are consistent with the theoretical analysis in terms of the outage probability, the BER and the average channel capacity. It is also shown that the full spatial diversity order can always be obtained at the signal-to-noise ratio (SNR) range of [0dB, 15dB] in the presence of multiple potential relays.

Intensity-Modulated Radiotherapy versus Three-Dimensional Conformal Radiotherapy in Definitive Chemoradiotherapy for Cervical Esophageal Squamous Cell Carcinoma: Comparison of Survival Outcomes and Toxicities

Nai-Bin Chen,Bo Qiu,Jun Zhang,Meng-Yun Qiang,Yu-Jia Zhu,Bin Wang,Jin-Yu Guo,Ling-Zhi Cai,Shao-Min Huang,Meng-Zhong Liu,Qun Li,Yong-Hong Hu,Qi-Wen Li,Hui Liu 대한암학회 2020 Cancer Research and Treatment Vol.52 No.1

Purpose The purpose of this study was to compare the survival and toxicities in cervical esophageal squamous cell carcinoma (CESCC) treated by concurrent chemoradiothrapy with either three-dimensional conformal radiotherapy (3D-CRT) or intensity-modulated radiotherapy (IMRT) techniques. Materials and Methods A total of 112 consecutive CESCC patients were retrospectively reviewed. 3D-CRT and IMRT groups had been analyzed by propensity score matching method, with sex, age, Karnofsky performance status, induction chemotherapy, and tumor stage well matched. The Kaplan- Meier method and Cox proportional hazards model were used for overall survival (OS) and progression-free survival (PFS). Toxicities were compared between two groups by Fisher exact test. Results With a median follow-up time of 34.9 months, the 3-year OS (p=0.927) and PFS (p=0.859) rate was 49.6% and 45.8% in 3D-CRT group, compared with 54.4% and 42.8% in IMRT group. The rates of grade ! 3 esophagitis, grade ! 2 pneumonitis, esophageal stricture, and hemorrhage were comparable between two groups, while the rate of tracheostomy dependence was much higher in IMRT group than 3D-CRT group (14.3% vs.1.8%, p=0.032). Radiotherapy technique (hazard ratio [HR], 0.09; 95% confidence interval [CI], 0.01 to 0.79) and pretreatment hoarseness (HR, 0.12; 95% CI 0.02 to 0.70) were independently prognostic of tracheostomy dependence. Conclusion No survival benefits had been observed while comparing IMRT versus 3D-CRT in CESCC patients. IMRT with fraction dose escalation and pretreatment hoarseness were considered to be associated with a higher risk for tracheostomy dependence. Radiation dose escalation beyond 60 Gy should be taken into account carefully when using IMRT with hypofractionated regimen.

Predictive V16alue of Thymidylate Synthase Expression in Gastric Cancer: A Systematic Review with Meta-analysis

Hu, Hua-Bin,Kuang, Lei,Zeng, Xiao-Min,Li, Bin,Liu, En-Yi,Zhong, Mei-Zuo Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.1

Purpose: The relationship between thymidylate synthase (TS) expression and outcomes in gastric cancer (GC) patients remains controversial, although most studies reported poor survival and reduced response to fluoropyrimidine were related to high TS in tumors. We carried out a systematic review of the literature with meta-analysis to estimate the predictive value of TS expression from published studies. Methods: We indentified 24 studies analysing the outcome data in gastric cancer stratified by TS expression. Effect measures of outcome were hazard ratios (HRs) for overall survival (OS) and event-free survival (EFS), or the odds ratio (OR) for overall response rate (ORR). HRs and ORs from these eligible studies were pooled using random-effects meta-analysis. Results: Fifteen studies investigated outcomes in a total of 844 patients with advanced GC, and nine studies investigated outcomes in a total of 1,235 patients with localized GC undergoing adjuvant therapy. Meta-analysis of estimates showed high TS expression was significantly associated with poor OS in the advanced setting (HR: 1.43, 95%CI: 1.08 - 1.90), and poor EFS in the adjuvant setting (HR: 1.53, 95%CI: 1.01 - 2.32). Subgroup analysis demonstrated TS expression to haves even greater value in predicting OS, EFS and ORR in advanced GC patients treated with fluoropyrimidine monotherapy (HR for OS: 2.32, 95%CI: 1.53 - 3.50; HR for EFS: 1.76, 95%CI: 1.19 - 2.60; OR for ORR: 0.32, 95%CI: 0.11 - 0.95). Conclusion: High levels of TS expression were asssociated with a poorer OS for advanced GC patients compared with low levels. In the adjuvant setting, high TS expression was also associated with a worse EFS. Additional studies with consistent methodology are needed to define the precise predictive value of TS.

Fire performance of concrete-filled steel tubular columns strengthened by CFRP

Zhong Tao,Zhi-Bin Wang,Lin-Hai Han,Brian Uy 국제구조공학회 2011 Steel and Composite Structures, An International J Vol.11 No.4

With the increasing use of concrete-filled steel tubes (CFST) as structural members, there is a growing need to provide suitable measures for possible strengthening or repair of these kinds of structural elements. Fibre reinforced polymer (FRP) jacketing is a recent method and is particularly attractive in which it does not significantly increase the section size, and is relatively easy to install. Thus, it can be used to enhance strength and/or ductility of CFST members. Very little information is available on the performance of FRP-strengthened CFST members under fire conditions. This paper is an attempt to study the fire performance of CFST columns strengthened by FRP. The results of fire endurance tests on FRP-strengthened circular CFST columns are presented. Failure modes of the specimens after exposure to fire, temperatures in the cross section, axial deformation and fire resistance of the composite columns are analysed. It is demonstrated that the required fire endurance can be achieved if the strengthened composite columns are appropriately designed.

ENERGY CONSUMPTION ANALYSIS OF DIFFERENT BEV POWERTRAIN TOPOLOGIES BY DESIGN OPTIMIZATION

Bin Wang,David Ling-Shun Hung,Jie Zhong,Kwee-Yan Teh 한국자동차공학회 2018 International journal of automotive technology Vol.19 No.5

Flexible layout of electric motors in battery electric vehicles (BEVs) has enabled different powertrain topologies to be used. However, these different powertrain topologies also affect the overall efficiency of energy conversion from the electrochemical form stored in the battery to the mechanical form on the driving wheels for vehicle propulsion. In this study, a methodology combining an energy-based BEV simulation model with the genetic algorithm optimization approach is applied to evaluate the energy efficiency of three different BEV powertrain topologies. The analysis is carried out assuming two different urban driving conditions, as exemplified by the New European Drive Cycle (NEDC) and the Japanese JC08 drive cycle. Each of the three BEV powertrain topologies is then optimized − in terms of its electric motor size and, where applicable, gear reduction ratio − for minimum energy consumption. The results show that among the three powertrain topologies, the wheel-hub drive without gear reducers consumes the least energy. The energy consumption of BEVs under the more aggressive JC08 drive cycle is consistently 8 % above that under NEDC for all three powertrain topologies considered.

Clinical Study of Hepatectomy Combined with Jianpi Huayu Therapy for Hepatocellular Carcinoma

Zhong, Chong,Li, Hui-Dong,Liu, Dong-Yang,Xu, Fa-Bin,Wu, Jian,Lin, Xue-Mei,Guo, Rong-Ping Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.14

Background: Traditional Chinese Medicine (TCM) possesses several advantages for treating patients with hepatocellular carcinoma (HCC). The theory of 'Jianpi Huayu Therapy' rooted from 'Jin Kui Yao Lue'is one of the most important therapies in this respect. This study was conducted to investigate the clinical effect and safety of hepatectomy combining with 'Jianpi Huayu Therapy' in the treatment of HCC. Materials and Methods: One hundred and twenty patients with HCC were randomized allocated into hepatectomy combined with 'Jianpi Huayu Therapy' group (treatment group, n=60) and hepatectomy alone group (control group, n=60). Disease- free survival (DFS) and overall survival (OS) were the primary end-points. Liver function at the end of one week after surgery, complications, average days of hospitalization as well as performance status (PS) at the end of one month post operation were also compared. Results: No significant differences existed between two groups on baseline analysis (p>0.05). No treatment related mortality occurred in either group. Post-operative complications were detected among 14 patients (23.3%) in the treatment group, and 12 (20.0%) in the control group (p=0.658). Alanine aminotransferase (ALT) at the end of one week after operation was lower in the treatment than control groups (p=0.042). No significant differences in other indexes of liver function were discovered between two groups. Average days of hospitalization reduced by 0.9 day in treatment group than in control (p=0.034). During follow-up, 104 patients (86.6%) developed recurrence. The rates of 1-, 3-, and 5-year DFS and median DFS for all patients were 77.4%, 26.3%, 9.0% and 25.6 months (range, 6.0~68.0), respectively (78.2%, 29.2%, 14.3% and 28.7 months for the 48 patients in the treatment group and 75.0%, 23.3%, 6.4%, and 22.6 months for the 56 patients in the control group (p=0.045)). 101 patients had died at the time of censor, with 1-, 3-, and 5-year overall survival rates and median survival for all patients of 97.5%, 76.4%, 40.5% and 51.2 months (range, 10.0~72.0), respectively (98.3%, 78.0%, 43.6% and 52.6 months, for treatment and 96.7%, 74.7%, 37.4%, and 49.8 months, for controls, respectively (p=0.048)). Conclusions: Hepatectomy combined with 'Jianpi Huayu therapy'was effective in the treatment of HCC, and reduced post-operative recurrence and metastasis and improved DFS and OS of HCC patients.

Sparse Channel Estimation of Single Carrier Frequency Division Multiple Access Based on Compressive Sensing

Zhong, Yuan-Hong,Huang, Zhi-Yong,Zhu, Bin,Wu, Hua Korea Information Processing Society 2015 Journal of information processing systems Vol.11 No.3

It is widely accepted that single carrier frequency division multiple access (SC-FDMA) is an excellent candidate for broadband wireless systems. Channel estimation is one of the key challenges in SC-FDMA, since accurate channel estimation can significantly improve equalization at the receiver and, consequently, enhance the communication performances. In this paper, we study the application of compressive sensing for sparse channel estimation in a SC-FDMA system. By skillfully designing pilots, their patterns, and taking advantages of the sparsity of the channel impulse response, the proposed system realizes channel estimation at a low cost. Simulation results show that it can achieve significantly improved performance in a frequency selective fading sparse channel with fewer pilots.