P.K肥料 및 栽培時期의 差異에 따른 쌀 Amylose含量의 變異

崔震龍,金正敎,許文會 慶尙大學校 1976 論文集 Vol.15 No.-

쌀의 amylose 含量이 栽培環境 特히 P·K 肥料와 栽培時期에 따라서 어떻게 變異하는가를 究明하기 爲해서 P·K 를 0.12, 24kg/10a의 3水準으로 하고 N를 12kg/10a로 固定시킨 후, 栽培時期를 普通期와 晩期로 區分하고, 6個 品種/系統을 使用하여 행한 試驗에서 얻은 結果를 要約하면 다음과 같다. 1. 쌀 amylose含量 P·K 肥料에 따라서 各品種의 反應에는 一定한 傾向을 發見할 수 없었다. 2. 쌀 amylose含量은 P·K 肥料處理에 依하여 有意的인 變異를 나타내지 못하였다. 3. 晩期栽培한 벼는 普通期裁培한 벼에 비하여 쌀 amylose含量이 增加하였다. In order to find out the effect of P and K ferilizers and cultivating seasons onthe variability of amylose aontent in rice(Oryza sativa L.) 9 treatment composed of teh combination of three levelsof phosphorous and potassium ferilizers at the fixed nitrogen level were made in two cultivating seasons, ordinary and late, with six varieties/lines. The results obtained are summarized as follows: 1. Any tendency was not found among the responses of amylose content of varieties/lines to P·K fertilizations. 2. Amylose content of rice grain was not significantly variated by P.K treatments. 3. Late season cultture showed higher amlylose content than ordinary one.

綠豆의 草型別 遺傳分散과 環境分散의 推定値利用에 關한 硏究 : Ⅰ. Genotypic correlation, path-coefficient and multiple regression 第1報 遺傳相關·經路係數·多重回歸

高美錫,崔震龍 慶尙大學校 1976 論文集 Vol.15 No.-

多收性 綠豆品種育成을 爲한 基礎情報를 얻기 爲하여 IMN의 31個 品種을 使用하여 遺傳相關, 表現型相關 및 遺傳力을 計算하고, 國內外에서 募集한 288個 品種을 莖長과 粒重의 差異에 따라 6個 品種君으로 區分하고 각각의 品種君에 對하여 形質相互間의 相關係數, 각각의 形質이 收量에 미치는 영향에 대한 經路係數를 分析하고 이를 다시 多重回歸分析을 通하여 檢計하였던 바 本 試驗에서 얻은 結果를 要約하면, 1. 遺傳型相關이 表現型相關보다 높게 나타났으며, 開花日數와 株當?數, 莖長과 莖直徑, 莖長과 株當分數間, 莖直徑과 株當分伎數, 株當分伎數와 株當分數間에는 高度의 正의 相關이 認定되었고, 開花日數와 收量 株當分伎數와 ?當粒數, 株當分數間와 100粒重, 株當分數間와 收量, 株當?數와 收量 및 株當?數와 100粒重間에는 高度의 負의 相關이 認定되었다. 2. 遺傳力이 높은 形質은 100粒重, 開花日數였으며 낮은 形質은 株當?數, ?當粒數 莖直徑이었다. 3. 莖長, 株當?數 種實收量은 그 變異가 크게 나타났다. 4. 收量이 높은 品種의 莖長은 70∼90cm의 範圍내에 分布하고 있었으며, 100립중은 3.5g과 6.5g를 중심으로 높은 수량을 나타내는 品種이 分布하고 있었다. 5. 各形質수는 相互間의 相關은 全체品種을 草型別로 分類하였을 경우 매우 낮은 값을 나타내엇으며 全체的으로 開花日數는 收量에 negative로 作用하였다. 莖長이 짧은 品種群에서는 莖長, 100粒重의 收量에 對한 直接效果가 크게 나타났으며 粒重이 가벼운 品種群에서는 莖長이, 粒重이 무거운 品種群에서는 株當分群에서는 100粒重이 收量에 對하여 보다큰 直接效果를 나타내었으며 이상의 결과는 多重回歸分析에 依하여 再確認되었다. In order to obtain a fundamental information to beused in the breeding of high yielding mungbean variety, 31 cultivars from the International Mungbean Nurseries wer eused to estimate genotypic and phenotypic correlation and heritability and the other 288 cultivars consised of domestic and foregn sources were classified into six varietal groups according to the plant height and 100-grain weight. For each varietal group correlation and path-coefficient among some agronomical characters were observed, and the resutls were reassered by computing multiple regression coefficients. The results are summarized as follows: 1. Highly positive genetic correlation coefficients were obtained between days to flowering and no. of pods/hkll, no. of branches/hill and no. of pods/hill, adn highly negative genotypic correlation coefficient wer found between days to flowering and yield, no. of branches/hill and 100-grain weight, no. of branches/hill and yield, no. of pods/hill and yield, and no. of pos/hill and 100-grain weight. 2. Heritabilities of 100-grain weight, days to flowering showed the highest and those of no. of pods/hill. adn stem diameter showed the lowest. 3. Plant height, no. of pods/hill, grain yield were found to be the most variable characteris in the different varietal groups. 4. The cultivars showing relatively high yielding capacity were distributed from 70cm to 90cm in plant height and 3.5 adn 6.5g in 100-grain weight. 5. The most simple correlation coefficient between characters were analysed to be lower in each varietal groups, and from the path-coefficient analysis, days to flowering were found to have a negative direct effect on grain yield. 6. Important characters seemed to be emphasized for selection in each varietal groups were supposed to be plant height and 100-grain weight in the short varietal groups; plant height in the light varietal groups; and no. of branches/hill and 100-grain weight in the heavy varietal group; and these results were confirmed by the estimation of multiple regression coefficients for each characters.

安全多收性 小豆品種 育成에 關한 硏究 : 第1報 年次別 小豆 諸形質의 遺傳力, 遺傳相關 및 經路係數의 變動 Ⅰ. Changes of Heritabilities, Genotypic and Phenotypic Correlation and Path Coefficient of Each Character of Phaseolus angularis Cultivated under the Different Meteorological Conditions

韓鏡秀,崔震龍 慶尙大學校 1975 論文集 Vol.14 No.-

安全 多收性 小豆 品種 育成을 위한 기초정보를 얻기 위하여 기상조건이 상이한 1967년과 1974년에 小豆 40여 품종을 재배하고 각 形質의 遺傳力, 遺傳型 相關 및 表現型 相關을 分析 比較하고, 經路係數 分析을 통하여 年次別 차이의 원인을 究明하였다. 本試驗에서 얻어진 결과를 要約하면 : 1. 각 形質의 遺傳力은 年次間 차이가 있었으며 특히 莖直徑, 莖長의 遺傳力은 甚한 차이를 나타내었고 開化期, 100粒重은 가장 높은 遺傳力을 나타내었다. 2. 100粒重, 開化日數, 莖長 및 生體重등과 收量間의 遺傳型相關은 1967년에는 높은 負의 相關을, 1974년에는 높은 正의 相關을 나타내었고, 開化日數, 莖長, 生體重과 1株莢數와의 遺傳型相關도 年次別로 같은 경향을 나타내었다. 3. 各形質間의 遺傳型相關의 年次別 差異를 path coefficient 分析을 통하여 直接效果를 間接效果로 분할하였던 바 1967년의 100粒重, 開化日數, 莖長, 生體重등과 收量間의 遺傳型相關이 높은 負의 相關을 나타낸 것은 이들 各形質이 收量에 대한 直接的인 影響이 아니고 1株莢數를 통한 간접적인 효과에 基因하고 있음을 발견하였다. 4. 1株莢數와 收量間에는 가장 높은 遺傳型 相關을 나타내고 있고 年次別 차이는 크지 않았으며 또한 他形質을 통한 間接 效果가 아니고 1株莢數의 收量에 대한 直接效果였다. 이와 같은 結果는 安全多收性 小豆品種 育成을 위하여는 1株莢數가 많은 個體를 選拔한다면 育種效率을 높일 수 있으며 開化日數와 100粒重은 初期世代에 選拔할 수 있는 形質로 結論 내릴 수 있었다. To obtain the basic information for breeding stable and high yielding varieties of adzuki bean(Phoseohis angularis W. F. WIGHT) above 40 varieties were tested at Jinju in the two crop years, 1967 and 1974, which showed a typical different meteorological data. Pod number per hill, 100-grain weight, days to flowering from sawing, stem diameter, stem length and plant weight were examined in each year, and their heritabilities, genotypic and phenotypic correlations and path coefficients were estimated. The results obtained are summarized as follows: 1. Heritabilities of stem diameter and stem length were found to be different between the crop years, and the highest heritabilities were estimated in days to flowering and 100-grain weight. 2. Genotypic correlations of 100-grain weight days to flowering, stem length, and plant weight to grain yield and those of days to flowering, stem length and plant weight to pod number were highly significantly negative in 1907 and highly significantly positive in 1974. 3. By partitioning the correlations by means of a path coefficient analysis method, the highly negative correlations between 100-grain weight, days to flowering, stem length, plant weight and grain yield in 1967 were attributed to the indirect effect of pod number to each character. 4. The most affectable character to grain yield was the pod number per plant. It showed the highest and most stable genotypic correlation, and the correlation was analyzed to be found the direct effect of the pod number to grain yield rather than the indirect effects of other characters to yield. These results give a conclusion that the individuals with a large number of pad are preferable to be selected forbreeding the stable and high yielding varieties of adzuki bean, and that two characters, days to flowering and 100-grain weight, have a benefit to be selected in earlier generation in the breeding program.

大豆栽培의 省力化를 爲한 除草劑의 效果

張權烈,韓鏡秀,高美錫,崔震龍 慶尙大學校 1972 論文集 Vol.11 No.-

以上의 結果를 要約하면 아래와 같다. 1. 雜草發生率은 品種間에는 有意性이 없으며 약제간에는 Mon가 7%, Lark 31%, MA 32%로서 Mon가 雜草發生이 제일 낮았다. 2. 雜草重은 品種間에는 有意性이 없으며 약제간에는 Mon 12.5kg, LA 13.7kg, MA 15kg로서 Mon가 제일 적었다. 3. 除草劑의 처리에 의한 種實重量은 관행구에 비하여 Mon은 56.8%, MA는 55.9%가 감소 되었고 LA는 가장 낮은 46.7%가 감소 되었다. 4. 經濟性 分析의 結果 10a當 순수익으로 LA 3,463원 MA 2,736원 Mon 2,641원으로서 관행구의 868원에 비하여 除草劑의 처리에 의하여 순수익을 높일 수 있었으므로 除草劑의 導入이 時急하다. The objective of this study was to evaluate the three herbicides, Machete, Mon-097 EC, and Lasso for annual weed control in soybeans at the experimental farm of Gyeong-Sang College. The results were summarized as follows; 1. The ratio of weed was not shown significance among the varietieties, as shown Tables 1 and 2, but the significance was shown among the herbicides and the plot of Mon-097 herbicide showed lowest value, 7%, as shown in Table 1. 2. The weight of weed also was not shown the significance among the varieties but the significance was shown among the herbicides, such as shown in Tables 1 and 2. The weight of weed also showed the same tendency as the ratio of weeds. 3. The yields of treated soybeans showed lower yield than normal condition plot, such as Mon-51.8%, Ma-55.9%, La-46.7%, respectively. 4. According to the analysis of economic values, herbicide plots were got more net-income than normal condition plot. It is needless to say that herbicides had to be recommendable in this case.

Alkali붕괴도검정을 위한 최적조건에 대하여

Zhin Ryong Choe,Mun Hue Heu 韓國作物學會 1975 Korean journal of crop science Vol.- No.19

백미의 alkali 붕괴도 청정에 필요한 최적조건을 구하기 위하여 test-tube miller를 사용하여 도정하고 25º , 30º , 35º 및 40℃ 의 온도에서, 1.4%에서 2.8%까지 8수준의 KOH 농도로 일정한 시간을 두고, Indica, Japonica등 10품종을 사용하여 alkali붕괴도 시험을 행하여, 본 정 검정에 영향을 미치는 백미의 도정 정도, KOH 농도 처리온도 및 처리시간을 점토하였다. 그 결과를 요약하면 다음과 같다. 1. Test-tube miller에서 90분간 도정한 시료는 alkali검정에 적당한 도정도를 나타내었다. 2. Japonica와 Indica를동시에 검정할 경우에는 1.8%의 KOH농도에서, Japonica만을 검정할 경우에는 1.4% 혹은 그 이하의 농도에서, 또 Indica만을 검정할 경우에는 2.2%의 농도에서 각각 30℃ , 18시간 동안 처리하는 것이 최적 조건으로 보였다. In other to determine the optimum condition for the alkali digestibility test, the milling degree of samples,the concentrations of potassium hydroxide,the temperature and the length of time for soaking were discussed.The results obtained from these experiment are summarized as follows; 1.Over 90 minute-milled samples in a new test tube miller showes the stable alkali digestibility values. 2.The optimum conditions for the alkali digestibility test were shown as 1.8 percent for the simultaneous treatment of Indica and Japonica varieties,1.4 percent or below for only Japonica and/or soft group varieties,and 2.2 percent of KOH for only Indoca and/ot hard group varieties provided the temperature and soaking time were fixed at ~;30~circ and 18 hours respectively.

Optimum Conditions for Alkali Digestibility Test in Rice

Zhin Ryong Choe,Mun Hue Heu 한국작물학회 1975 한국작물학회지 Vol.19 No.1

Evaluation of white clover and rye grown in rotation with no-tilled rice

Zhin Ryong Choe,Young Son Cho,Kazumasa. Hidaka,Takuya. Mineta 한국육종학회 2003 한국육종학회 학술발표회 발표요지 Vol.35 No.3

Heterosis Evaluation of Chemical-Induced Hybrid Seeds of Wheat Via Micro Plot Technique and Mitochondrial Complementation

CHOE, ZHIN RYONG,YANG, MIN SUK,KIM, SUK HYUN 경상대학교 유전공학연구소 1989 遺傳工學硏究所報 Vol.8 No.-

F_1 seeds, obtained from four sources of chemical-induced male sterility were categorized into three groups according to outcross rate(10, 20, 30%), were grown in the field with 10×10 cm distance based on the microplot technique. Heterosis values were compared with mitochondrial complementation(MC) value expressed as oxidase acitivity of cytochrome C of single and mixture of motochondria of parental seedlings. Heterosis on the number of tillers per plant was not consistently high in the crosses and irregularly affected by the outcross rate. Heterosis values of 100 grain weight and grain yield(expressed as panicle weight) were high. The relationship between heterosis and MC values was not confirmed.

Developement of hybrid-seed production system in self-pollinated crops by chemical-induced male sterility

CHOE, ZHIN RYONG,YANG, MIN SUK,KIM, SEOK HYEON,OH, HO SANG 경상대학교 유전공학연구소 1988 遺傳工學硏究所報 Vol.7 No.-

In order to investigate the effects of chemicals on electrophoretic properties of leaf protein, rice seedling(cv. Dongjin) at 4-leaf stage was foliar-applicated by four chemcials and a relationship between the effect of chemicals on the alteration in banding proteins was examined. Among the chemicals 2000ppm(a. i.) sodium arsenite caused an alteration in banding proteins(MW 45000), but the alteration was proved not to coincide with grain sterility of outcross rates. The same chemicals were foliar applicated to female parents of wheat and barley at a late-booting stage, which were grown under the different distance and orientation from pollinators. Grain sterility and outcross rate were investigated by the enveloping method. The effects of orientation of pollinators adn cross direction on grain sterility or outcross rates were inconsistant in different cross combinations. However, the distance of poollinators proved to be great importance in hybrid-seed production in self-pollinated crops.

The Inheritance of Amylose Content and Alkali Digestibility Value of Rice (Oryza sativa L.)Applied as "Waxy Carrier Technique"

CHOE,Zhin Ryong,HEU,Mun Hue,LEE,Eun Woong 서울大學校 農科大學 1977 서울대농학연구지 Vol.2 No.1

본 硏究는 (1) 米質 特히 食味와 관계되는 두가지 主要形質인 amylose含量과 알칼리崩壞度(ADV)에 미치는 環境의 影響을 空明하고 (2) 새로이 提唱된 waxy carrier technique理論에 根據하여 쌀벼와 메벼의 交雜에서 나온 메벼가 이들 形質을 母本으로부터 어떻게 傳授 받는가를 밝히고 (3)이 理論을 應用하여 含量이 다른 두 메 品種의 F₁을 찰벼와 戾交雜하고 거기서 나온 순수 메벼의 amylose含量의 分離比를 검토하여 쌀 amylose含量의 遺傅에 관한 情報를 얻기 위한 目的으로 實施되었다. 쌀 amylose 함량 및 알칼리 崩壞度에 미치는 환경,特히 溫度의 영향을 空明하기 위하여 인공기상실에서 진흥, 유신 및 IR-30의 3品種을 出穗期에서 5 혹은 10일간 또 蒸發器에 7일간 15℃ 혹은 30℃에 처리한 것을 自然條件에서 栽培한 것과 비교하였고(試驗 I), 밀양 23호(메벼)와 Wx 317-4-7-4(찰벼)의 交雜에서 나온 F₂개체의 出穗期를 精密히 조사하고 homozygous nonwaxy F₂와 waxy F₂를 분리하여 그 종자를 여러 濃度의 KOH용액에서 알칼리 崩壞度를 검정하고 出穗期와 알칼리 崩壞度 간의 관계를 空明하였다.(試驗 II). Waxy carrier technique에 근거하여 쌀의 amylose 함량 및 알칼리 崩壞度가 母本으로부터 雜種世代에 移轉하는 원리를 空明하기 위하여 두 찰벼(Wx 316-9-10-1-2와 Wx 199-33-6-1-3)를 amylose 함량과 ADV가 相異한 4가지 메벼와 交雜하고 거기서 나온 찰메에 대하여 homozygous인 F₂개체중에서 amylose含量을 메벼에 한하여, 알칼리 崩壞度를 메와 찰을 같이 사용하여 檢定하였다(試驗 Ⅲ). 또 waxy carrier technique 理論을 應用하여 쌀 amylose 含量의 遺傅에 관한 정보를 얻기 爲하여 amylose含量이 다르고 標識形質(marker character)을 가진 8개 isogenic line을 交雜하고 이들F1을 所謂 短桿찰계통(semi-dwarf waxy line)에 戾交雜, 그 後代에서 나온 메벼의 amylose 含量을 分析하여 그 分離상태를 검토하였다(試驗 Ⅳ). 以上의 4가지 試驗에서 얻은 結果를 要約하면 다음과 같다. (1) 出穗期에 5日 혹은 10日간 30℃에 처리하였을 때 amylose 含量은 有意的인 變化가 없었으나 알칼리 붕괴도는 有意的으로 減少하였다. 같은 時期와 기간동안 15℃에 처리하였을 때에는 自然상태의 것과 비교할 때 amylose 함량이나 ADV에서 有意的인 差異가 認定되지 않았다. (2) 알칼리 붕괴도는 밀양 23호와 Wx 317의 교잡에서 나온 homozygous nonwaxy F₂개체 혹은 메 母本의 경우에는 1.5%, homozygous waxy F₂개체 혹은 찰 母本의 경우는 1.6% KOH濃度에서 각각을 檢定암이 妥當하였고, 出穗期와 알칼리 崩壞度 間에는 有意的인 正의 相關이 認定되었다. (3) 메나 찰의 交雜에서 나온 homozygous 메 F₂개체의 amylose含量은 메母本의 amylose함량과 같은 정도로 回復되었으나 알칼리 崩壞度는 메와 찰에서 매우 큰 變異를 나타내었다. 그러나 育種的인 見地에서 母本의 알칼리 崩壞度를 回復할 수 있는 個體의 選拔이 可能할 것으로 보였다. (4) Amylose함량이 다른 isogenic line을 상호 交雜하고 이 F₁을 몇가지 찰과 檢定交配하여 여기서 나온 雜種後期世代에서 分離한 메벼의 amylose함량을 分析한 결과 모든 供試組合에서 1 : 1 로 分離하는 二頂分布(bimodal distribution)를 나타냄으로써 쌀 amylose含量은 한쌍의 單純遺傳子에 으하여 支配된다는 것을 確認하였다. The main purpose of this study was (1) to determine the envirnmental influence on two major quality charcters related to cooking and eating qualities of rice, i.e., amylose content and alkali digestibility values(ADV), and (2) to evaluate the genetical behavior of these characters by applying the waxy carrier technique. For the study on the environmental influence on these characters, three varieties, Jinheung, Yushin and IR-30, were tested in the different temperatural conditions at maturation, and the relationship berween the heading date and ADV was estimated in the cross between Milyang 23 and Wx317-4-7-4. For the study on the transmittance of amylose content and ADV based on the waxy carrier technique, ADV-different two waxy lines, and four near-isogenic nonwaxy lines were used. And finally to evaluate the genetical behavior of amylose-different eight issogenic lines with typical base color s a marker were used as amylose parents. The results obtained from these four experiments are summarized as follows: (1) When the rice plants were placed in 30℃ for 5 of 10 days at heading, the samples did not show any significant changes in amylose content but showed significant changes in ADV. And when the plants were placed at the same temperature for 7 days at ripening stage, the samples showed significantly low amylose content and low ADV. Amylose content of samples tested in 15℃ for the same periods showed no significant difference compared to those in the natural condition. (2) In a cross between Milyang 23 and Wx317, F₂plants with homozygous nonwaxy endosperm and nonwaxy parent showed significant postive correlation between heading date and ADV in 1.5% KOH at 30℃, while F₂plants with homozygous waxy endosperms and waxy parent showed such positive correlation in 1.6% KOH at 30℃. (3) In the crosses between nonwaxy and waxy parents, the amylose content of nonwaxy parents was transmitted to the F₂plant whith homozygous nonwaxy endosperms, which is coincident with the principle of waxy carrier technique. However, ADV was not so simple to apply the waxy carrier technique due to the large variation in ADV of homozygous F₂plants. (4) When F₁lines derived from the nonwaxy amylose-different parents were testcrossed to waxy lines, the isolated homozygous nonwaxy plants showed segregation of 1 : 1 in amylose content, showing bimodal distributions in all combinations tested. This result confirmed that the amylose content in ricd is controlled by one pair of major genes.