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Zhibing Chang,Mo Chu,Chao Zhang,Shuxia Bai,Hao Lin,Liangbo Ma 한국화학공학회 2017 Korean Journal of Chemical Engineering Vol.34 No.12
Thermal bitumen is an important intermediate derived from kerogen decomposition during oil shale pyrolysis. In this study, free bitumen (FB) and bound bitumen (BB) were obtained by extracting oil shale chars (300- 550 oC) before and after demineralization, and then analyzed by liquid chromatography fractionation, Fourier transform infrared spectroscopy, and gas chromatography/mass spectrometry. The FB yield first increased and then decreased with increasing temperature, and the maximum value was 2.10% at 400 oC. The decarboxylation of acids and decomposition of esters at 350-450 oC decreased the content of these compounds. Meanwhile, the intense cracking reactions of aliphatic compounds and alkyl chains at 400-450 oC decreased the carbon chain lengths and molecular weights of these compounds. From the analytical results obtained for the BB fractions, we suggest that some carboxylic acids or carboxyl group-containing compounds may be trapped on carbonate particles by the formation of Ca2+COO− bonds, whereas other oxygenated compounds (e.g., esters and phenols) can be adsorbed preferentially by clay minerals through Lewis acid-base interactions.
Design of On-line UPS Based on Boost Converter
Chunyang Gu,Zhibing Yang,Chunling Yang,Mengda Yang 제어로봇시스템학회 2009 제어로봇시스템학회 국제학술대회 논문집 Vol.2009 No.8
Design and build up a new on-line digital UPS based on boost topological structure with a new kind of single-chip computer, STC12C5412AD, realizing sine wave and square wave output, and shifting among 50Hz, 60 Hz, and 400Hz of the supply for adopting into different industrial standards and applications. Such UPS system contains the module of intelligent charging from storage battery and thorough security functions, characterized by the output of un-interruption and high stability, low distortion, free of interference, low cost and multi-function.
The FvCYP714C2 gene plays an important role in gibberellin synthesis in the woodland strawberry
Xiaofan Guo,Zhibing Xie,Yang Zhang,Shouming Wang 한국유전학회 2021 Genes & Genomics Vol.43 No.1
Background Fragaria vesca, the woodland strawberry, is a diploid relative of the cultivated strawberry. A GA-defcient mutant was found in ethyl methanesulfonate (EMS)-mutagenized lines of the Fragaria vesca accession ‘Yellow Wonder’. Objective CYP714C2 was found to be diferentially expressed using RNA-seq analysis. It is necessary to identify the function of this gene. Methods In order to identify the function of this gene, it was cloned and transformed into Arabidopsis thaliana. Results The DNA sequence of CYP714C2 was found to be 1940 bp in length, with an open reading frame (ORF) of 1539 bp that is predicted to encode a protein of 512 amino acids. The hydrophilicity of this protein is low and it is unstable. The highest relative expression of FvCYP714C2 was found in the leaves, followed by the pedicels, and low expression levels were found in the other tissues examined. Constitutive expression of FvCYP714C2 signifcantly promoted the growth of transgenic A. thaliana plants; transgenic Arabidopsis plants grew faster and grew well than wild type Col-0 plants. GA1+3 contents of the genetically modifed Arabidopsis lines were signifcantly higher than that in the wild type. Conclusion We conclude that FvCYP714C2 is a gene that functions in the gibberellin biosynthesis pathway in strawberry.
Liao, Chao-Jan,Lai, Zhibing,Lee, Sanghun,Yun, Dae-Jin,Mengiste, Tesfaye American Society of Plant Biologists 2016 The Plant cell Vol.28 No.7
<P>Arabidopsis thaliana HOOKLESS1 (HLS1) encodes a putative histone acetyltransferase with known functions in seedling growth. Here, we show that HLS1 regulates plant responses to pathogens and abscisic acid (ABA) through histone acetylation at chromatin of target loci. The hls1 mutants show impaired responses to bacterial and fungal infection, accelerated senescence, and impaired responses to ABA. HLS1 modulates the expression of WRKY33 and ABA INSENSITIVE5 (ABI5), known regulators of pathogen and ABA responses, respectively, through direct association with these loci. Histone 3 acetylation (H3Ac), a positive mark of transcription, at WRKY33 and ABI5 requires HLS1 function. ABA treatment and pathogen infection enhance HLS1 recruitment and H3Ac at WRKY33. HLS1 associates with Mediator, a eukaryotic transcription coregulatory complex, through direct interaction with mediator subunit 18 (MED18), with which it shares multiple functions. HLS1 recruits MED18 to the WRKY33 promoter, boosting WKRY33 expression, suggesting the synergetic action of HLS1 and MED18. By contrast, MED18 recruitment to ABI5 and transcriptional activation are independent of HLS1. ABA-mediated priming of resistance to fungal infection was abrogated in hls1 and wrky33 mutants but correlated with ABA-induced HLS1 accumulation. In sum, HLS1 provides a regulatory node in pathogen and hormone response pathways through interaction with the Mediator complex and important transcription factors.</P>
Ballistic electronic and thermal conductance of monolayer and bilayer black phosphorus
Zexiang Deng,Huanjun Chen,Zhibing Li,Weiliang Wang 한국물리학회 2017 Current Applied Physics Vol.17 No.2
We performed first principle calculations based on density functional theory and GW approximation with Landauer approach to study ballistic electronic conductance and ballistic thermal conductance of black phosphorus (BP) whose dimensions are smaller than the mean free path of carriers. Ballistic electronic conductance of monolayer BP and bilayer BP with four different stacking styles, and ballistic thermal conductance of monolayer BP were investigated. The electronic transmission value along armchair direction is found to be a little higher than that along zigzag direction near the band gap energy range. Our calculations showed that, the G point hole effective mass along zigzag direction is stackingstyle sensitive, while other G point effective mass is independent of stacking style. We also reported the anisotropic ballistic thermal conductance of monolayer BP: the anisotropic ballistic thermal conductance ratio of zigzag-to-armchair approaches a constant (about 2.0) when the temperature is beyond 200 K. The reasons for these properties were discussed.
Identification and Functional Characterization of R3 MYB Transcription Factor Genes in Soybean
Shucai Wang,Jinsong Pang,Hongwei Xun,Zhibing Zhang,Yunxiao Zhou,Xueyan Qian,Yingshan Dong,Xianzhong Feng,Bao Liu 한국식물학회 2018 Journal of Plant Biology Vol.61 No.2
In Arabidopsis, trichome formation is regulatedby a MYB-bHLH-WD40 (MBW) transcriptional activatorcomplex, which can activate the expression of GLABRA2(GL2) and R3 MYB genes. GL2 is required for trichomeformation, whereas R3 MYBs inhibit trichome formation byblocking the formation of the MBW complex, thus inhibitingthe expression of GL2. By using the amino acid sequence of theArabidopsis R3 MYB transcription factor TRICHOMELESS1(TCL1) to BLAST the soybean (Glycine max) protein database,we found that there are a total of six R3 MYB genes insoybean, namely Glycine max TRICHOMELESS1 through 6(GmTCL1-GmTCL6). By generating transgenic plants, wefound that trichome formation in soybean plants overexpressingeach of the GmTCLs remained largely unchanged, and theexpression of putative GL1 and GL2 genes in the transgenicplants was unaffected. However, all the GmTCLs interactedwith GLABRA3 (GL3) in transfected Arabidopsis protoplasts,expression each of the GmTCLs in Arabidopsis inhibitedtrichome formation, and the expression levels of GL1 andGL2 were greatly reduced in the Arabidopsis transgenicplants. Moreover, phenotypic complementary analysis showedthat GmTCL1 is functionally equivalent to TCL1. Takentogether, these results suggest that GmTCLs are functionalR3 MYBs, however, they do not regulate trichome formationin soybean.
Yanwei Ji,Yang Xu,Yanping Li,Zhui Tu,Zhibing Huang,Xue Liu,Da Lei 한국식품과학회 2013 Food Science and Biotechnology Vol.22 No.1
The purpose of this study was to develop a membrane filtration method to isolate uninuclei conidium of Aspergillus oryzae, then the method was adopted to develop a transformation system of A. oryzae. A. oryzae 3.951 contained 1–4 nuclei in each conidium. The percentages of uninucleate and binucleate conidia were approximately 16.15 and 74.22%, respectively. Conidia suspension was filtrated with a 5-μm membrane to overcome the bottleneck caused by multinucleate conidia and to remove excess multinucleate conidia before UV mutagenesis. Uninucleate conidia of 5-fluoroorotic acid (5-FOA)-resistant strains were enriched by filtration with a 3-μm membrane. The pyrG mutant strain AS11 was obtained and GFP-pyrG was successfully transformed into AS11.
Xiao Tang,Kun Tong,Lishan Zhu,Guoqing Fu,Wei Chang,Ting Zhou,Zhibing Zhang,Ling Tong,Ling Zhang,Yuqin Shi,Ling Zhang,Yuqin Shi 대한독성 유전단백체 학회 2016 Molecular & cellular toxicology Vol.12 No.4
Di-2-ethylhexyl phthalate (DEHP) which belongs to phthalatic-acid esters, is a kind of harmful, global environmental pollutants. It is a known endocrine disrupting chemical and male reproductive toxicant. However, the mechanism by which DEHP exposure result in male reproductive toxicity is still unclear. To elucidate the productive toxicity mechanism of DEHP, we attemptted to investigate oxidative stress, apoptotic effects, mRNA and protein expression of apoptosis-associated genes including FasL, caspase-3, and caspase-8 in GC-2spd (mouse spermatogenic cells). The results showed that, with the increase of DEHP concentration, cell apoptosis rate increased; the activities of relation index of oxidative stress such as malondialdehyde (MDA), superoxide dismutase (SOD) and glutatione peroxidase (GSH-PX) changed significantly; the mRNA and protein expression levels of FasL, caspase-3, -8, altered obviously. These results suggested that DEHP could induce apoptosis of GC-2spd cells through oxidative stress and FasL-dependent pathway.