Identifying the geographical origin of protected sea cucumbers (Apostichopus japonicus) in China using random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR)

Zhenyu Yun,Zhao Sun,Haiyan Xu,Zhihong Sun,Yao Zhang,Zhen Liu 한국식품과학회 2017 Food Science and Biotechnology Vol.26 No.2

Dalian sea cucumber, Yantai sea cucumber, and Weihai sea cucumber, which belong to Apostichopus japonicus, are protected as geographical indications in China based on their high nutritional values and medical propertys. The 26 samples, including Dalian sea cucumbers (9 samples) in Liaoning province, Yantai sea cucumbers (9 samples), and Weihai sea cucumbers (8 samples) in Shandong province, were individually collected from the designated geographical sea areas and the genetic relationships and DNA polymorphisms were evaluated by random amplified polymorphic DNA technology and gene segments sequencing. The RAPD dendrogram showed that the genetic diversity of the three types of sea cucumbers was rich. The neighbor-joining tree shows that the genetic relationship of the samples from the adjacent sea areas is closer. It demonstrates that the gene characteristics of sea cucumbers from different sea areas were obvious and the genetic diversity analysis by RAPD-PCR can be used as a rapid method for geographical discrimination.

Microbially induced calcium precipitation based anaerobic biosynthetic crystals for removal of F− and Ca2+ in groundwater: Performance optimization, kinetics, and reactor operation

Zhenyu Zhai,Amjad Ali,Junfeng Su,Zhenle Hao,Jiaran Liu,Zhao Wang 한국화학공학회 2022 Korean Journal of Chemical Engineering Vol.39 No.11

Anaerobic biosynthetic crystals (ANBC) were prepared based on microbially induced calcium precipitation (MICP) and their potential explored for groundwater defluoridation and decalcification. The preparation conditions of ANBC were optimized and the influence of key factors (initial fluoride ions (F−) concentration, pH, and initial calcium ions (Ca2+) concentration) on the crystals was investigated. During the operation of the reactor, at pH of 7.0, the hydraulic retention time (HRT) of 6 h, and Ca2+ concentration of 180 mg L−1, a maximum removal efficiency reached 93.31%, while 66.20% of Ca2+ could be removed. The adsorption dynamics study showed that the adsorption of ANBC was most in line with the pseudo-second-order model. The stability of ANBC operation was studied and failure reaction showed that the crystals maintained a stable removal ability after 35 times of repeated use. Further studies found that this was attributed to the continuous growth and synthesis of the crystals. The defluoridation and decalcification mechanism was further explored by scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), and X-ray diffraction spectra (XRD). This study innovatively proposes a method for biosynthesis of crystals under anaerobic conditions based on MICP, which can efficiently and stably remove F− and Ca2+ in groundwater, providing a valuable strategy for groundwater contaminant remediation and energy saving.

Multi-Scale Simulation of an Immobilizing F1-ATPase Molecular Motor

Zhenyu Yang,Ya-Pu Zhao 한국물리학회 2008 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.52 No.4

Immobilization, including specific attachment, positioning and orientation, is essential for the integration of F₁-ATPase molecular motors into nanoelectromechanical systems (NEMS). The stability of the immobilization is a very critical technology to prolong the functional lifetime of the molecular motor. Hybrid quantum mechanics and molecular mechanics (QM/MM) and classical molecular dynamics (MD) simulations were separately implemented to investigate the immobilization of the F₁-ATPase molecular motor on different substrates. The QM/MM simulation was employed to give insight into the interaction between the (Histidine)6-tagged peptide and the nickel-nitrilotriacetate (Ni-NTA). The analysis of the charge redistribution revealed that the Ni ion increased the negative charge on the nitrogen atoms of imidazoles, resulting in a strong chemical bonding. The maximum torque that the His-tagged peptide could apply to the rotary molecular motor was estimated from the results of the MD simulation. The immobilization stability was analyzed and is discussed. Immobilization, including specific attachment, positioning and orientation, is essential for the integration of F₁-ATPase molecular motors into nanoelectromechanical systems (NEMS). The stability of the immobilization is a very critical technology to prolong the functional lifetime of the molecular motor. Hybrid quantum mechanics and molecular mechanics (QM/MM) and classical molecular dynamics (MD) simulations were separately implemented to investigate the immobilization of the F₁-ATPase molecular motor on different substrates. The QM/MM simulation was employed to give insight into the interaction between the (Histidine)6-tagged peptide and the nickel-nitrilotriacetate (Ni-NTA). The analysis of the charge redistribution revealed that the Ni ion increased the negative charge on the nitrogen atoms of imidazoles, resulting in a strong chemical bonding. The maximum torque that the His-tagged peptide could apply to the rotary molecular motor was estimated from the results of the MD simulation. The immobilization stability was analyzed and is discussed.

Research on the Predictive Optimal PID Plus Second Order Derivative Method for AGC of Power System with High Penetration of Photovoltaic and Wind Power

Xilin Zhao,Zhenyu Lin,Bo Fu,Li He,Chaoshun Li 대한전기학회 2019 Journal of Electrical Engineering & Technology Vol.14 No.3

Because of the uncertainty of the external environment, high penetration of renewable energy such as wind power and solar energy in the modern power system renders the traditional automatic generation control (AGC) methods more challenging. An improved AGC method named predictive optimal proportional integral diff erential plus second order derivative (POPID + DD) for multi-area interconnected grid is proposed in this paper to reduce the negative impacts of the uncertainty which is caused by the high penetration of renewable energy. Firstly, the mathematical model of the AGC system of multiarea power grid with penetration of photovoltaic (PV) and wind power is built. Then, PO-PID + DD controller is presented to improve the system robustness with respect to system uncertainties. In order to obtain the predictive sequence of the integral system output, the characteristic of the controller is included in the system model. Thus, according to the predictive sequence and designed objective function, the input of the controller can be readjusted to obtain the optimal eff ect of AGC. An IEEE 39-bus system is introduced as an example to testify the feasibility and eff ectiveness of the proposed method. The simulation results indicate that the system controlled by the proposed controller has desired dynamic performances.

The Influence of Vacuum Packaging of Hot-Boned Lamb at Early Postmortem Time on Meat Quality during Postmortem Chilled Storage

Yingxin Zhao,Li Chen,Heather L. Bruce,Zhenyu Wang,Bimol C. Roy,Xin Li,Dequan Zhang,Wei Yang,Chengli Hou 한국축산식품학회 2022 한국축산식품학회지 Vol.42 No.5

To evaluate the effects of early postmortem vacuum packaging (VP) on meat quality during postmortem chilled storage, hot-boned lamb was vacuum-packaged at 1, 6, 12, 24, and 48 h postmortem and stored around 2℃ until 168 h postmortem, with lamb packaged in plastic wrap as the control (aerobic packaging). Intramuscular pH decline was delayed when lamb was vacuum packaged at 1, 6, and 12 h postmortem (p<0.05). The lamb vacuum-packaged at 1 h postmortem (VP-1h group) had significantly lower shear force values and purge losses accompanied by lower free thiol group values than other treatments during postmortem storage and was also higher in extractable calpain-1 activity by 6 h postmortem (p<0.05). Free thiol group concentrations were significantly higher after VP at 6 and 12 h postmortem (p<0.05). Packaging lamb under vacuum very early postmortem produced the lowest shear force and purge loss, likely by slowing heat loss and muscle temperature decline, implying that lamb quality is improved by VP when applied very early postmortem. This was at the expense of protein oxidation, which was unrelated to other meat quality measurements, most likely because potential contracture during hot boning confounded its impact. Further research is required to understand the implications of the interaction between protein oxidation, VP, and hot boning on the acceptability of lamb.

The Rice YGL Gene Encoding an Mg2+-chelatase ChlD Subunit is Affected by Temperature for Chlorophyll Biosynthesis

Banpu Ruan,Zhenyu Gao,Juan Zhao,Bin Zhang,Anpeng Zhang,Kai Hong,Shenglong Yang,Hongzhen Jiang,Chaolei Liu,Guang Chen,Youlin Peng,Guojun Dong,Longbiao Guo,Zhengjin Xu,Qian Qian 한국식물학회 2017 Journal of Plant Biology Vol.60 No.4

Leaf color-related mutants are important materialsto study the chlorophyll biosynthesis and chloroplastdevelopment. Here, we isolated and characterized a thermosensitiveyellow-green leaf mutant, ygl, through mutagenizedJaponica rice cultivar Zhonghua11 by EMS. The mutantshowed yellow-green leaves only at the seedling stage. Genetic and molecular analyses uncovered that YGL encodean Mg2+-chelatase ChlD subunit, which was a new allele ofChl1/YGL7. Quantitative PCR and GUS staining showedthat the YGL transcripts expressed in all tested organs andsubcellular localization detected YGL protein in the chloroplasts. The mutant displayed yellow-green leaves at 20°C andnormal green leaves at 34°C during the seedling stage. Transcript level of YGL in the ygl mutant was significantlyreduced at 20°C, and increased at 34°C compared with thewild-type plant. Furthermore, the expression of chlorophyllbiosynthesis and chloroplast development related genes wereinfluenced by the mutation of YGL at different temperature. These findings suggested that the YGL gene not only play akey role in chlorophyll biosynthesis, but is affected attranscript level by temperature in rice.

A NEWTON-IMPLICIT ITERATIVE METHOD FOR NONLINEAR INVERSE PROBLEMS

Meng, Zehong,Zhao, Zhenyu The Korean Society for Computational and Applied M 2011 Journal of applied mathematics & informatics Vol.29 No.3

A regularized Newton method for nonlinear ill-posed problems is considered. In each Newton step an implicit iterative method with an appropriate stopping rule is proposed and analyzed. Under certain assumptions on the nonlinear operator, the convergence of the algorithm is proved and the algorithm is stable if the discrepancy principle is used to terminate the outer iteration. Numerical experiment shows the effectiveness of the method.

A NEWTON-IMPLICIT ITERATIVE METHOD FOR NONLINEAR INVERSE PROBLEMS

Zehong Meng,Zhenyu Zhao 한국전산응용수학회 2011 Journal of applied mathematics & informatics Vol.29 No.3

A regularized Newton method for nonlinear ill-posed problems is considered. In each Newton step an implicit iterative method with an appropriate stopping rule is proposed and analyzed. Under certain assumptions on the nonlinear operator, the convergence of the algorithm is proved and the algorithm is stable if the discrepancy principle is used to terminate the outer iteration. Numerical experiment shows the effectiveness of the method.

DOB-based piezoelectric vibration control for stiffened plate considering accelerometer measurement noise

Li, Shengquan,Zhao, Rong,Li, Juan,Mo, Yueping,Sun, Zhenyu Techno-Press 2014 Smart Structures and Systems, An International Jou Vol.14 No.3

This paper presents a composite control strategy for the active suppression of vibration due to the unknown disturbances, such as external excitation, harmonic effects and control spillover, as well as high-frequency accelerometer measurement noise in the all-clamped stiffened plate. The proposed composite control action based on the modal approach, consists of two contributions including feedback part and feedforward part. The feedback part is the well-known PID controller, which is widely used to increase the structure damping and improve its dynamic performance close to the resonance frequencies. In order to get better performance for vibration suppression, the weight matrixes is optimized by chaos sequence. Then an improved disturbance observer (IDOB) as the feedforward compensation part is developed to enhance the vibration suppression performance of PID under various disturbances and uncertainties. The proposed IDOB can simultaneously estimate the various disturbances dynamically as well as measurement noise acting on the system and suppress them by feedforward compensation design. A rigorous analysis is also given to show why the IDOB can effectively suppress the unknown disturbances and measurement noise. In order to verify the proposed composite control algorithm (IDOB-PID), the dSPACE real-time simulation platform is used and an experimental platform for the all-clamped stiffened plate active vibration control system is set up. The experimental results demonstrate the effectiveness, practicality and strong anti-disturbances ability of the proposed control strategy.

Design and Implementation of an AHRS Based on Gauss Newton and Complementary Filtering Algorithm

ZhiMin Liu,Zhao Kai Ning,Feng Tian,ZhenYue Pang 보안공학연구지원센터 2016 International Journal of Hybrid Information Techno Vol.9 No.8

This paper presents design and implementation of an attitude and heading reference system (AHRS) based on Gauss Newton and Complementary Filtering algorithm (CF). The algorithm uses data measured from the MEMS sensor which contains a three-axis magnetometer, a three-axis angular rate sensor, and a three-axis accelerometer. The filter represents rotations using quaternions rather than Euler angles, which eliminates the long-standing problem of singularities associated with attitude estimation. A process model for rigid body angular motions and angular rate measurements is defined. The process model converts angular rates into quaternion rates, which are integrated to obtain quaternions. The Gauss-Newton iteration algorithm is utilized to find the optimal quaternion that relates the measured accelerations and earth magnetic field in the body coordinate frame to calculated values in the earth coordinate frame. Then fuse the optimal quaternion with the quaternion updated from gyroscope and calculate the attitude angle based on the complementary filtering algorithm . Extensive testing of the filter have proved feasibility and acceptable performance of this AHRS design.