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( Hui fang Xie ),( Qi Li ),( Min Min Wang ),( Lin Guo Zhao ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.6
The recombinant strain P. pastoris GS115-lccC was used to produce laccase with high activity. Factors influencing laccase expression, such as pH, methanol concentration, copper concentration, peptone concentration, shaker rotate speed, and medium volume were investigated. Under the optimal conditions, laccase activity reached 12,344 U/L on day 15. The recombinant enzyme was purified by precipitating and dialyzing to electrophoretic homogeneity, and was estimated to have a molecular mass of about 58 kDa. When guaiacol was the substrate, the laccase showed the highest activity at pH 5.0 and was stable when the pH was 4.5~6.0. The optimal temperature for the laccase to oxidize guaiacol was 60℃, but it was not stable at high temperature. The enzyme could remain stable at 30℃ for 5 days. The recombinant laccase was used to degrade chlorpyrifos in several laccase/mediator systems. Among three synthetic mediators (ABTS, HBT, VA) and three natural mediators (vanillin, 2,6-DMP, and guaiacol), vanillin showed the most enhancement on degradation of chlorpyrifos. Both laccase and vanillin were responsible for the degradation of chlorpyrifos. A higher dosage of vanillin may promote a higher level of degradation of chlorpyrifos, and the 2-step addition of vanillin led to 98% chlorpyrifos degradation. The degradation of chlorpyrifos was faster in the L/V system (kobs = 0.151) than that in the buffer solution (kobs = 0.028).
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Estimation of load and resistance factors based on the fourth moment method
Zhao-Hui Lu,Yan-gang Zhao,Alfredo H-S. Ang 국제구조공학회 2010 Structural Engineering and Mechanics, An Int'l Jou Vol.36 No.1
The load and resistance factors are generally obtained using the First Order Reliability Method (FORM), in which the design point should be determined and derivative-based iterations have to be used. In this paper, a simple method for estimating the load and resistance factors using the first four moments of the basic random variables is proposed and a simple formula for the target mean resistance is also proposed to avoid iteration computation. Unlike the currently used method, the load and resistance factors can be determined using the proposed method even when the probability density functions (PDFs) of the basic random variables are not available. Moreover, the proposed method does not need either the iterative computation of derivatives or any design points. Thus, the present method provides a more convenient and effective way to estimate the load and resistance factors in practical engineering. Numerical examples are presented to demonstrate the advantages of the proposed fourth moment method for determining the load and resistance factors.
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AERODYNAMIC ANALYSIS OF A DUCTED FAN VTOL UAV FOR TRANSITION MANOUEVRE
Hui Wen Zhao,Cees Bil,Bok-Hyun Yoon 한국항공우주학회 2008 한국항공우주학회 학술발표회 논문집 Vol.- No.-
The development of a dynamic model for a new ducted fan VTOL UAV is presented to analyze the transition maneuver from vertical to horizontal flight. The objective is to ensure that the UAV can achieve a smooth transition from vertical climb or hover ing to horizontal flight by using conventional control surfaces which are affected by the propeller exit flow behind the duct. As a pan of the development of the dynamics model, this paper presents a simplified duct design methodology based on an annular wing theory and explains how the slipstream affects control surface and generates high efficiency lift force. The theoretical results are to be compared with CFD analysis. Computational fluid dynamics was used to evaluate the aerodynamic coefficients at different speeds and angles of attack. The interaction between the fan flow and duct causes complex flow structures depending on the flight condition. To evaluate the aerodynamic characteristics of the duct, 3D analytical models were used. The full-scale model of UAV with due ted fan rotating is also simulated using computational fluid dynamics approach in hopes of analyzing the process of UAV in full flow field.
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Zhao, H.,Hui, K.S.,Hui, K.N. Pergamon Press ; Elsevier Science Ltd 2014 Carbon Vol.76 No.-
We reported on the facile synthesis of N-doped multilayer graphene (N-MLG) from milk powder that uses melamine as a nitrogen-doping source with Fe<SUP>2+</SUP> ions as catalytic growth agents. We showed that milk powder could be used as a precursor for large-scale N-MLG synthesis through heat treatment at 1000<SUP>o</SUP>C under N<SUB>2</SUB> atmosphere for 45-120min. In addition, heating time has a remarkable effect on N content and type in N-MLG. The resulting N-MLG exhibited higher catalytic activity than undoped graphene and carbon nanotubes (CNTs), as well as comparable catalytic activity to commercial Pt/C catalyst toward oxygen reduction reaction (ORR). Furthermore, the catalytic activity was sensitive to N content and type, particularly the ratio of pyridinic-N to total N atoms. Results showed that Fe atoms in N-MLG were found to function not as synergetic catalysts for ORR but as catalytic growth agents for N-MLG formation, thereby promoting and stabilizing N atoms. The present method could lead to the synthesis of bulk amounts of N-MLG, which is promising for applications in electrochemical energy devices, such as fuel cells and metal-air batteries.
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Hui-Xin Zhao,제해금 한국유통경영학회 2022 유통경영학회지 Vol.25 No.1
Purpose: The purpose of this study was to examine how green credit loans affect the credit risk of commercial banks in China. The heterogeneity of banks was analyzed, and the synergy with the equity capital ratio was analyzed more innovative in consideration of the impact of the government’s regulatory policy on green credit loans. Research design, data, and methodology: This study focuses on the influence of green credit on the risk of commercial banks in China. Using the sample data of 16 relatively large commercial banks in China from 2009 to 2019, this paper constructs a static panel data model for empirical analysis. Results: First, The results show that the green credit business can significantly reduce the non-performing loan ratio (NPLR) of banks. Second, We found that the green credit ratio (GCR) is positively correlated with the NPLR namely, the green credit ratio and credit risk show a "U-shaped" relationship between credit risk and "pre-down, post-up". Third, The impact of green credit on the credit risk of non-state-owned banks is significantly reduced, while the impact on the credit risk of state-owned commercial banks is not significant, Finally, In view of the interaction of capital adequacy ratio(CAR) and green credit on bank credit risk, we find that banks with higher capital adequacy ratio have a more positive effect on reducing credit risk. Implications: Commercial banks that develop the green credit have risk consideration, especially credit risk. It should be noted that risk management considerations are important for the stability of the banking system. This study provides empirical evidence for commercial banks to reduce the credit risk. The results of this study are helpful in actively promoting China’s green credit loan policy.
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Zhao, Yaying,Zhao, Guangbo,Sun, Rui,Liu, Hui,Wang, Zhuozhi,Sihyun, Lee,Kong, Ming American Chemical Society 2017 ENERGY AND FUELS Vol.31 No.4
<P>Two typical types of Chinese lignite samples were employed to investigate the coal drying characteristics of an innovative COMBDry lignite drying system. The drying rate increased significantly with an increase in drying temperature and ratio of the flue gas, to the lignite. Using a nitrogen adsorption instrument, it was found that the drying process promoted generation and enlargement of the surface pore structures of the particles, which can enhance coal combustion rate. A horizontally fixed bed furnace and a SIGNAL S4i pulsar NDIR (infrared (IR)) gas analyzers were used to investigate the combustion characteristics of coal samples after the drying treatment. In an attempt to analyze the variation in the sample surface chemical structure during the drying process, Fourier transform infrared (FT-IR) spectrometry and Raman spectroscopy were employed for the investigation. The results showed that the concentration of aliphatic hydrogen decreased with an increase in drying temperature and resulted from the decomposition of oxygen-containing complexes (released CO and CO2) and reaction with hydroxyl groups. The number of functional groups decreased when the drying temperature exceeded 210 degrees C. The amount of carbonyl and carboxylic esters initially increased and then (210 degrees C) decreased with an increase in flue gas temperature, and the content of aromatic carbon was unchanged with the treatment. The ordered crystalline carbon changed into the crystal defect structure and amorphous carbon and the degree of graphitization decreased and thus the combustion reactivity of the dried lignite was improved.</P>
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Zhao Hui Liu,Si Yu Zhang,Yong Yang Yu,Guo Qiang Su,G. Q. SU 대한독성 유전단백체 학회 2015 Molecular & cellular toxicology Vol.11 No.4
(-)-4-O-(4-O-β-D-glucopyranosylcaffeoyl) quinic acid (GQA), new compound isolated from Lonicera japonica, was found with the activity of inhibiting HIF-1. GQA exhibited anti-proliferative effects on a broad spectrum of tumor cell lines in HIF-1 dependent mechanism and could induce HT-29 cell apoptosis through the mitochondrial pathway. Oral administration of GQA showed significant inhibition against tumor growth of human colon carcinoma xenografted in nude mice. For the first time, this study provides scientific rational to develop GQA as a novel class of antitumor agents against the human colon carcinoma.
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Zhao, Ming-Hui,Liang, Shuang,Guo, Jing,Choi, Jeong-Woo,Kim, Nam-Hyung,Lu, Wen-Fa,Cui, Xiang-Shun Cambridge University Press 2016 Microscopy and Microanalysis Vol.22 No.2
<B>Abstract</B><P>Iron is an essential trace element that plays important roles in the cellular function of all organs and systems. However, the function of Fe(II) in mammalian embryo development is unknown. In this study, we investigated the role of Fe(II) during preimplantation embryo development. Depletion of Fe(II) using thiosemicarbazone-24 (TSC24), a specific Fe(II) chelator, rescued quenching of the Fe(II)-sensitive fluorophore phen green-SK. After <I>in vitro</I> fertilization, TSC24 significantly reduced the cleavage rate as well as blastocyst formation. The hatch rate of blastocysts was also reduced with 1 pM TSC24 treatment (20.25±1.86 versus 42.28±12.96%, <I>p</I><0.05). Blastocysts were cultured in leukemia inhibitory factor-free mouse embryonic stem cell culture medium with or without TSC24, and those with depleted Fe(II) displayed delayed attachment and lost the ability to induce embryoid body formation. To further explore the mechanism of Fe(II) in embryo development, we assessed the expression of 5-hydroxymethylcytosine (5hmC) and OCT4 in the pronuclear and blastocyst stages, respectively. We observed that Fe(II) reduced 5hmC and OCT4 expression, which could be explained by low ten-eleven translocation (TET) enzyme activity induced by TSC24 treatment. These findings demonstrate that Fe(II) is required for mammalian embryo development and that it facilitates the process via regulation of TET activity.</P>
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