Intercalation of Biomolecules into NiAl-NO3 Layered Double Hydroxide Films Synthesized in Situ on Anodic Alumina/Aluminium Support

Hua-Zhang Zhao,Ying-Yue Chang,Jing Yang,Qin-Zheng Yang 대한금속·재료학회 2013 ELECTRONIC MATERIALS LETTERS Vol.9 No.2

Layered double hydroxide (LDH) films were synthesized in situ on anodic alumina/aluminium (AAO/Al). Glucose oxidase (GOD) and L-ascorbic acid (vitamin C, VC) were intercalated respectively into the in-situ grown LDH films by anion-exchange in aqueous solutions. Dodecylsulfate (SDS) was used to expand the lamellar structure before GOD intercalation into the LDH film. The resulting products were characterized by scanning electron microscopy (SEM), x-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy and thermo gravimetric analysis (TGA). The results showed that VC and GOD were successfully intercalated into the in-situ synthesized LDH film. These biomolecules loaded LDH films could have potential applications in electrode modification, safe storage and effective delivery of bioactive compounds.

Separation of arenols from a low-temperature coal tar by liquid-liquid extraction

Hua-Shuai Gao,Zhi-Min Zong,Zheng Yang,Dao-Guang Teng,Xiu-Hua Sun,Li Yan,Xian-Yong Wei,Qing-Jie Guo,Tian-Sheng Zhao,Hong-Cun Bai 한국화학공학회 2020 Korean Journal of Chemical Engineering Vol.37 No.5

Low-temperature coal tar (LTCT) is a sticky liquid mixture produced mainly from coal pyrolysis, which contains various value-added chemicals (VACs). Liquid-liquid extraction is considered as one of the green and effective ways to explore the organic composition and separate the VACs from LTCT. Herein, petroleum ether, methanol, and carbon disulfide were used to extract arenols from a LTCT. As a result, the relative content and absolute content of arenols extracted from the LTCT are ca. 96.3% and 85.9%, respectively. Among them, p-cresol is predominant, accounting for 22.2%. The isolated contents of arenols are up to 84.6%. Moreover, a kilogram-scale operation was carried out under the same conditions, which offers a potential application in industrial production.

A Fair Scheduling Algorithm for Input-Queued Switches

Hua-An ZHAO,Qingsheng HU 대한전자공학회 2009 ITC-CSCC :International Technical Conference on Ci Vol.2009 No.7

To increase both the capacity and the processing speed for input-queued (IQ) switches, we proposed a fair scalable scheduling architecture (FSSA) where several chips of cascaded sub-scheduler compose a large-scale high performance network scheduler. In this paper, we present an improved scheduling algorithm named DIFSSA where a distributed iteration scheme is employed based on FSSA. The simulation and measurement results show that DIFSSA achieves better performance on average delay and throughput under heavy loads compared to existing algorithms and its scheduler performance and the processing time are also better than the other. Moreover, it has been implemented by 4 Xilinx FPGAs as 64×64 switch whose data rates can be up to 800Mbps and the tradeoff between performance and hardware complexity has been solved peacefully.

Effect of sodium chloride on interaction between amino sulfonate amphoteric surfactant and octylphenol polyoxyethylene ether (10) in aqueous solution

Zhao Hua Ren 한국공업화학회 2015 Journal of Industrial and Engineering Chemistry Vol.30 No.-

In aqueous solution containing the NaCl of 0.25 mol/L, the micellization of binary mixtures of sodiumdodecyl diamino sulfonate (C12AS) and octylphenol polyoxyethylene ether (10) (OP-10) have beeninvestigated employing both the UV–vis spectroscopy method, using pyrene as a probe, and thetensiometric method. The values of critical micelle concentration (cmc) at different molar compositionsin the mixture of C12AS/OP-10 were determined by both the UV–vis spectroscopy measurement and thetensiometric measurement. Within the framework of pseudophase separation model, some parameters(including the ideal values of mixed cmc, the interaction parameters, components and activitycoefficients in mixed micelles, and thermodynamic parameters of micellization) were calculated usingvarious models and treatments, e.g. the regular solution theory, the ideal model from the Lang model, theRodenas’s treatment and the Motomura’s treatment, and the mechanism on effect of NaCl on theinteracting behavior between two surfactants was discussed. The results show that as compared to thecases in the absence of NaCl, the addition of NaCl enhances non-ideal mixing and synergism betweenC12AS and OP-10 in all mixed systems, mainly resulting from the decrease of the steric effect ofheadgroups of two surfactants and the increase of the ionic strength in aqueous solution. Thermodynamic data show that on adding NaCl the micellization for the mixtures of C12AS and OP-10 can proceed spontaneously, and is an enthalpy-driven process owing to the variation of hydrophobicproperties in aqueous solution.

Modulation of Drug Resistance in Ovarian Cancer Cells by Inhibition of Protein Kinase C-alpha (PKC-α) with Small Interference RNA (siRNA) Agents

Zhao, Li-Jun,Xu, Heng,Qu, Jun-Wei,Zhao, Wan-Zhou,Zhao, Yi-Bing,Wang, Jin-Hua Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.8

Objective: To determine whether silence of $PKC-{\alpha}$ expression by small interference RNA (siRNA) might regulate MDR1 expression and reverse chemoresistance of ovarian cancer. Methods: We measured gene and protein expression of MDR1 and $PKC-{\alpha}$ in ovarian cancer cells and assessed their correlation with cell drug resistance. We also examined whether blocking $PKC-{\alpha}$ by RNA interference (RNAi) affected MDR1 expression and reversed drug resistance in drug sensitivity tests. Results: The drug resistance cell lines, OV1228/DDP and OV1228/Taxol, had higher gene and protein expression of MDR1 and $PKC-{\alpha}$ than their counterpart sensitive cell line, OV1228. SiRNA depressed $PKC-{\alpha}$ gene protein expression, as well as MDR1 and protein expression and improved the drug sensitivity in OV1228/DDP and OV1228/Taxol cells. Conclusion: These results indicated that decreasing $PKC-{\alpha}$ expression with siRNA might be an effective method to improve drug sensitivity in drug resistant cells with elevated levels of $PKC-{\alpha}$ and MDR1. A new siRNA-based therapeutic strategy targeting $PKC-{\alpha}$ gene could be designed to overcome the chemoresistance of ovarian cancer.

Interacting behavior between amino sulfonate amphoteric surfactant and octylphenol polyoxyethylene ether (7) in aqueous solution and pH effect

Zhao Hua Ren 한국공업화학회 2014 Journal of Industrial and Engineering Chemistry Vol.20 No.5

The micellization properties of binary mixtures of sodium n-dodecyl diamine sulfonate (C12AS) and octylphenol polyoxyethylene ether (7), OP-7, in aqueous solution at the pH of 3.5, 6.0, and 8.5 have been characterized mainly employing both the UV–vis spectroscopy method, using pyrene as a probe, and the tensiometric method. The interaction parameters between two surfactants, the activity coefficients, the molar fractions of components in mixed micelles, and thermodynamic parameters obtained using some theoretical treatments including the regular solution theory (RST), the phase separation model, the Rubingh model, Maeda’s treatment, and so on, were evaluated and the effect of pH on the above parameters was also discussed. Reduction of the mixed critical micelle concentration and negative values of interaction parameter indicate synergistic effect between two surfactants for all mixed systems. The charge density and steric effect of head group of C12AS may play a vital role on the formation of mixed micelle, and the values of pH in aqueous solution can influence the ionic form of C12AS and this steric effect, thus resulting in different interacting behaviors between two surfactants. Thermodynamic data show that the process of micellization for all mixed systems is spontaneous, the stability of mixed micelle is mainly dependent on the components in bulk solution, and the more stable mixed micelle can form in neutral or weak acidic solution.

Microstructure and magnetic properties of nanocomposite permanent magnetic materials

Zhao-hua Cheng 한국자기학회 2002 한국자기학회 학술연구발표회 논문개요집 Vol.12 No.2

Associations of Plasma Glucagon Levels with Estimated Glomerular Filtration Rate, Albuminuria and Diabetic Kidney Disease in Patients with Type 2 Diabetes Mellitus

Hua-Xing Huang,Liang-Lan Shen,Hai-Yan Huang,Li-Hua Zhao,Feng Xu,Dong-Mei Zhang,Xiu-Lin Zhang,Tong Chen,Xue-Qin Wang,Yan Xie,Jian-Bin Su 대한당뇨병학회 2021 Diabetes and Metabolism Journal Vol.45 No.6

Background: Type 2 diabetes mellitus (T2DM) is characterized by elevated fasting glucagon and impaired suppression of postprandial glucagon secretion, which may participate in diabetic complications. Therefore, we investigated the associations of plasma glucagon with estimated glomerular filtration rate (eGFR), albuminuria and diabetic kidney disease (DKD) in T2DM patients.Methods: Fasting glucagon and postchallenge glucagon (assessed by area under the glucagon curve [AUCgla]) levels were determined during oral glucose tolerance tests. Patients with an eGFR <60 mL/min/1.73 m2 and/or a urinary albumin-to-creatinine ratio (UACR) ≥30 mg/g who presented with diabetic retinopathy were identified as having DKD.Results: Of the 2,436 recruited patients, fasting glucagon was correlated with eGFR and UACR (r=–0.112 and r=0.157, respectively; P<0.001), and AUCgla was also correlated with eGFR and UACR (r=–0.267 and r=0.234, respectively; P<0.001). Moreover, 31.7% (n=771) presented with DKD; the prevalence of DKD was 27.3%, 27.6%, 32.5%, and 39.2% in the first (Q1), second (Q2), third (Q3), and fourth quartile (Q4) of fasting glucagon, respectively; and the corresponding prevalence for AUCgla was 25.9%, 22.7%, 33.7%, and 44.4%, respectively. Furthermore, after adjusting for other clinical covariates, the adjusted odds ratios (ORs; 95% confidence intervals) for DKD in Q2, Q3, and Q4 versus Q1 of fasting glucagon were 0.946 (0.697 to 1.284), 1.209 (0.895 to 1.634), and 1.521 (1.129 to 2.049), respectively; the corresponding ORs of AUCgla were 0.825 (0.611 to 1.114), 1.323 (0.989 to 1.769), and 2.066 (1.546 to 2.760), respectively. Additionally, when we restricted our analysis in patients with glycosylated hemoglobin <7.0% (n=471), we found fasting glucagon and AUCgla were still independently associated with DKD.Conclusion: Both increased fasting and postchallenge glucagon levels were independently associated with DKD in T2DM patients.

Micellization behavior of binary mixtures of amino sulfonate amphoteric surfactant with different octylphenol polyoxyethylene ethers in aqueous salt solution: Both cationic and hydrophilic effects

Zhao Hua Ren,Jing Huang,Yue Luo,Yan Cheng Zheng,Ping Mei,Lu Lai,Yan Ling Chang 한국공업화학회 2016 Journal of Industrial and Engineering Chemistry Vol.36 No.-

The effect of different valent inorganic cations and the hydrophilic group of surfactant on themicellization behavior of binary surfactant mixtures constituted by an amphoteric surfactant, sodium3-(N-dodecyl ethylenediamino)-2-hydropropyl sulfonate (C12AS), and three nonionic surfactantsoctylphenol polyoxyethylene ether (OP-n) with different numbers of oxyethylene glycol ethers (n),namely, OP-10, OP-7, and OP-4 was investigated in aqueous solution. These inorganic cations includeNa+, K+, Mg2+, Ca2+, Ba2+, Al3+ and Fe3+. Both the tensiometry and the UV–vis spectrophotometry usingpyrene as a probe were adopted to determine the critical micelle concentrations (cmc) of individual ormixed surfactants. Interaction parameters between two surfactants and other parameters were obtainedbased on the regular solution theory, the pseudophase separation model, Rubingh’s model, etc. Thermodynamic parameters including Gibbs energy of micellization and thermodynamic stability werealso calculated by both the equation proposed by Molyneux et al. and Maeda’s treatment, respectively. The effect of different valent cations on both the mixed cmc of surfactant mixtures and the molar fractionof C12AS in mixed micelle (X1) can be explained theoretically by the salting-out effect, electrostaticinteraction and steric effect. For three binary surfactant mixtures, the chain length of hydrophilic groupof surfactant results in different micellization behaviors. On adding salts, the interaction parametersshow that there exists a synergistic effect between two surfactants, and the effect increases withincreasing the ratio of valence (Z) and atom radius (R) of cation, especially, for the C12AS/OP-4 mixture. With increasing the Z/R value of cation, a deviation of X1 from the ideal value shows a divergence forthree binary surfactant mixtures because of different hydrophilicities of surfactant. Thermodynamicparameters indicate that the addition of cations with a large Z/R value can be contributive moreeffectively to the formation of stable mixed micelle than the case in the presence of cations with a smallZ/R value.

Microstructure and Magnetic Properties of Nanocomposite Sm₂Fe15Ga₂Cx / α-Fe Permanent Magnets

Zhao-hua Cheng 한국자기학회 2003 Journal of Magnetics Vol.8 No.1

In our previous work, microstructure and magnetic properties of two-phase exchange-coupled Sm₂Fe_(15)Ga₂Cx/α-Fe nanocomposites have been investigated by means of x-ray diffraction, transmission electron microscopy and magnetization measurement. It was found the exchange coupling between the magnetically hard phase Sm₂Fe_(15)Ga₂Cx and the magnetically soft one α-Fe results in an enhancement of the remanence. The sizes of crystallites of both phases are, however, much larger than the Bloch domain-wall width of the magnetically hard phase. This microstructure gives rise to a concave demagnetization curve and consequently reduces the maximum energy product. In order to improve their magnetic properties, a few percent of Zr, which may be effective to refine the microstructure through rapid quenching, was introduced into the nanocomposites. The addition of Zr was found to improve the magnetic properties significantly. Under optimum heat-treatment conditions, the remanence, coercivity and maximum energy product increase from 0.65 T, 0.48 T and 50 kJ/㎥ for the Zr-free sample to 0.72 T, 0.77 T and 71.6 kJ/㎥ for the 1 at.% Zr-containing one, respectively. The improvements of magnetic properties are due to the refinement of microstructure by the addition of Zr.