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RISS 인기검색어
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- 저자 : Zhao Wenlan (검색결과 4 건)
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On Katherine Mansfield's Feminist Consciousness
Zhao Wenlan 청주대학교 국제협력연구원 2006 國際文化硏究 Vol.24 No.-
Katherine Mansfield contributed her short life to the short story creation and created many female images in her works. Although she is not a radical feminist theorist, her feminist consciousness makes her always concerned with women's fate and their pursuit. Combining her own life experience, she described women's sufferings in their life struggles, and portrayed women's loneliness, disillusionment and rebellion in the patriarchal society. Her stories make us feel Mansfield's concern with women problems and her exploration to women's emancipation, and also reveal her limitations in feminism.
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Analysis of Fuzhisan and Quantitation of Baicalin and Ginsenoside Rb1 by HPLC-DAD-ELSD
Jingkun Zhao,Desheng Wang,Shurong Duan,Jianxiu Wang,Jing Bai,Wenlan Li 대한약학회 2009 Archives of Pharmacal Research Vol.32 No.7
Fuzhisan (FZS) is a traditional Chinese medicine composed of Radix Ginseng Rubra (Araliaceae family), Scutellaria baicalensis Georgi (Labiatae family), Angelica sinensis (Oliv) Diels (Umbelliferae family), Anemone altaica Fisch. Ex C.A. Mey (Araceae family) and Glycyrrhiza uralensis (Leguminosae family). To establish the chemical fingerprint of the components of FZS and quantify the components, baicalin and ginsenoside Rb1, a high performance liquid chromatography method coupled with diode array and evaporative light scattering detectors (DAD-ELSD) was developed. Separation of 36 omponents from 12 batches of FZS was performed on a C18 column, with a mobile phase consisting of acetonitrile and 0.1% acetic acidwater, with gradient elution at a column temperature of 30℃. The optimum detection wavelength was set at 335 nm, the drift tube temperature of ELSD was set at 80℃, the carrier gas pressure was 25 psi, and the gain = 10. The similarity among 12 batches of FZS was over 0.95. Five constituents of FZS, namely baicalin, ferulic acid, and ginsenosides Rg1, Re, and Rb1, were identified based on their retention times (RT). Calibration curves for baicalin and ginsenoside Rb1 showed good linearity (r2 > 0.9992); recoveries ranged from 95% to 99%. This quantification method is reproducible and simple, and may provide a tool to assess the quality of FZS.
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Cooperation between Human DAF and CD59 in Protecting Cells from Human Complement-mediated Lysis
Xu, Li,Wu, Wenlan,Zhao, Zhouzhou,Shao, Huanjie,Liu, Wanhong,Liu, Hui,Li, Wenxin Korean Society for Biochemistry and Molecular Biol 2006 Journal of biochemistry and molecular biology Vol.39 No.6
The complement (C) regulatory proteins decay accelerating factor (DAF, CD55) and CD59 could protect host cells using different mechanisms from C-mediated damage at two distinct levels within the C pathway. Co-expression of DAF and CD59 would be an effective strategy to help overcome host C-induced xenograft hyperacute rejection. In this study, we made a construct of recombinant expression vector containing DAF and CD59 cDNA and the stable cell lines were obtained by G418 selection. Extraneous genes integration and co-expression were identified by PCR, RT-PCR and Western blot analysis. Human c-mediated cytolysis assays showed that NIH/3T3 cells transfected stably with pcDNA3-CD59, pcDNA3-DAF, and pcDNA3-CD59DAF-DP were protected from C-mediated damage and that synchronously expressed human CD59 and DAF provided the most excellent protection for host cells as compared with either human CD59 or DAF expressed alone. Therefore, the construct represents an effective and efficacy strategy to overcome C-mediated damage in cells and, ultimately, in animals.
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ZmFKBP20-1 improves the drought and salt tolerance of transformed Arabidopsis
Yanli Yu,Yanjiao Li,Fengjuan Jia,Meng Zhao,Wencai Li,Qi Sun,Nana Li,Wenlan Li,Zhaodong Meng 한국식물학회 2017 Journal of Plant Biology Vol.60 No.6
FK506-binding proteins (FKBPs), which belongto the peptidyl-prolyl cis/trans isomerase superfamily, areinvolved in plant response to abiotic stresses. A number ofFKBP family genes have been isolated in plants, but littlehas been reported of FKBP genes in maize. In this study, adrought-induced FKBP gene, ZmFKBP20-1, was isolated frommaize and was characterized for its role in stress responsesusing gene expression, protein subcellular localization,transformation in Arabidopsis, expression patterns of thestress-responsive genes, and physiological parameter analysis. During drought and salt stresses, ZmFKBP20-1 transgenicArabidopsis plants exhibited enhanced tolerance, which wasconcomitant with the altered expression of stress/ABAresponsivegenes, such as COR15a, COR47, ERD10, RD22,KIN1, ABI1, and ABI2. The resistance characteristics ofZmFKBP20-1 overexpression were associated with a significantincrease in survival rate. These results suggested thatZmFKBP20-1 plays a positive role in drought and salt stressresponses in Arabidopsis and provided new insights into themechanisms of FKBP in response to abiotic stresses inplants.
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