Cyclin D1 Gene G870A Variants and Primary Brain Tumors

Zeybek, Umit,Yaylim, Ilhan,Ozkan, Nazli Ezgi,Korkmaz, Gurbet,Turan, Saime,Kafadar, Didem,Cacina, Canan,Kafadar, Ali Metin Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.7

Alterations of cyclin D1, one of the main regulators of the cell cycle, are known to be involved in various cancers. The CCDN1 G870A polymorphism causes production of a truncated variant with a shorter half-life and thus thought to impact the regulatory effect of CCDN1. The aim of the present study was to contribute to existing results to help to determine the prognostic value of this specific gene variant and evaluate the role of CCDN1 G870A polymorphism in brain cancer susceptibility. A Turkish study group including 99 patients with primary brain tumors and 155 healthy controls were examined. Genotypes were determined by polymerase chain reaction-restriction fragment length polymorphism analysis. The CCDN1 genotype frequencies in meningioma, glioma and control cases were not significantly different (p>0.05). No significant association was detected according to clinical parameters or tumor characteristics; however, a higher frequency of AG genotype was recorded within patients with astrocytic or oligoastrocytic tumors. A significant association between AG genotype and gliobilastoma multiforme (GBM) was recorded within the patients with glial tumors (p value=0.048 OR: 1.87 CI% 1.010-3.463). According to tumor characteristics, no statistically significant difference was detected within astrocytic, oligoasltrocytic tumors and oligodentrioglias. However, patients with astrocytic astrocytic or oligoastrocytic tumors showed a higher frequency of AG genotype (50%) when compared to those with oligodendrioglial tumors (27.3%). Our results indicate a possible relation between GBM formation and CCDN1 genotype.

A NEW APPROACH FOR NUMERICAL SOLUTION OF LINEAR AND NON-LINEAR SYSTEMS

ZEYBEK, HALIL,DOLAPCI, IHSAN TIMUCIN The Korean Society for Computational and Applied M 2017 Journal of applied mathematics & informatics Vol.35 No.1

In this study, Taylor matrix algorithm is designed for the approximate solution of linear and non-linear differential equation systems. The algorithm is essentially based on the expansion of the functions in differential equation systems to Taylor series and substituting the matrix forms of these expansions into the given equation systems. Using the Mathematica program, the matrix equations are solved and the unknown Taylor coefficients are found approximately. The presented numerical approach is discussed on samples from various linear and non-linear differential equation systems as well as stiff systems. The computational data are then compared with those of some earlier numerical or exact results. As a result, this comparison demonstrates that the proposed method is accurate and reliable.

A NEW APPROACH FOR NUMERICAL SOLUTION OF LINEAR AND NON-LINEAR SYSTEMS

HALIL ZEYBEK,IHSAN TIMUCIN DOLAPCI 한국전산응용수학회 2017 Journal of applied mathematics & informatics Vol.35 No.1

In this study, Taylor matrix algorithm is designed for the approximate solution of linear and non-linear differential equation systems. The algorithm is essentially based on the expansion of the functions in differential equation systems to Taylor series and substituting the matrix forms of these expansions into the given equation systems. Using the Mathematica program, the matrix equations are solved and the unknown Taylor coecients are found approximately. The presented numerical approach is discussed on samples from various linear and non-linear differential equation systems as well as stiff systems. The computational data are then compared with those of some earlier numerical or exact results. As a result, this comparison demonstrates that the proposed method is accurate and reliable. In this study, Taylor matrix algorithm is designed for the approximate solution of linear and non-linear dierential equation systems. The algorithm is essentially based on the expansion of the functions in dierential equation systems to Taylor series and substituting the matrix forms of these expansions into the given equation systems. Using the Mathematica program, the matrix equations are solved and the unknown Taylor coecients are found approximately. The presented numerical approach is discussed on samples from various linear and non-linear dierential equation systems as well as stiff systems. The computational data are then compared with those of some earlier numerical or exact results. As a result, this comparison demonstrates that the proposed method is accurate and reliable.

Financial toxicity in patients with gynecologic malignancies: a cross sectional study

Burak Zeybek,Emily Webster,Natalia Pogosian,Joan Tymon-Rosario,Alan Balch,Gary Altwerger,Mitchell Clark,Gulden Menderes,Gloria Huang,Masoud Azodi,Elena S. Ratner,Peter E. Schwartz,Alessandro D. Santin 대한부인종양학회 2021 Journal of Gynecologic Oncology Vol.32 No.6

Objective: To evaluate financial toxicity and assess its risk factors among patients with gynecologic cancers. Methods: This is a cross sectional study that included 2 survey tools, as well as patient demographics, disease characteristics, and treatment regimen. Financial toxicity is measured by validated Comprehensive Score for Financial Toxicity (COST) tool. Participants were also asked to complete a 55-question-survey on attitudes and perspectives surrounding cost of care. Descriptive statistics was used to report patient demographics. Spearman's rank correlation was calculated to assess the relation between financial toxicity and patient/ disease related variables. Graphpad Prism Software Version 8.0 was used for analyses. Results: A total of 50 patients with various gynecologic malignancies were enrolled. Median COST score was 20.5 (range, 1–33). Sixty-five percent of the patients reported being in debt due to their cancer care and 4% filed bankruptcy. Correlation analysis showed that COST score was correlated with age (r=−0.3, p=0.028), malignancy type (r=0.3, p=0.039) and income (r=0.3, p=0.047). Ovarian cancer patients had significantly less financial toxicity (median COST score=23) when compared to patients with other gynecologic malignancies (median COST score=17, p=0.043). When scores were dichotomized into low (score ≥22) and high toxicity (score <22), 58% (29/50) of the patients were noted to have high financial toxicity. Enrollment to a clinical trial did not significantly alleviate financial burden. Conclusion: Financial toxicity is a significant burden even among highly insured gynecologic oncology patients. Age, malignancy type and income were correlated with high financial burden.

Investigation of ICAM-1 and β3 Integrin Gene Variations in Patients with Brain Tumors

Yilmaz, Umit,Zeybek, Umit,Kahraman, Ozlem Timirci,Kafadar, Ali Metin,Toptas, Bahar,Yamak, Nesibe,Celik, Faruk,Yaylim, Ilhan Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.10

Background: Primary brain tumors constitute a small percent of all malignant cancers, but their etiology remains poorly understood. ${\beta}3$ integrin (ITGB3) has been recognized to play influential roles in angiogenesis, tumor growth and metastasis. Intercellular adhesion molecule-1 (ICAM-1) is a surface glycoprotein important for tumor invasion and angiogenesis. The aim of this study was to investigate whether specific genetic polymorphisms of ICAM-1 and ITGB3 could be associated with brain cancer development and progression in a Turkish population. Our study is the first to our knowledge to investigate the relationship between brain tumor risk and ICAM-1 and ${\beta}3$ integrin gene polymorphisms. Materials and Methods: The study covered 92 patients with primary brain tumors and 92 age-matched healthy control subjects. Evaluation of ${\beta}3$ integrin (Leu33Pro (rs5918)) and ICAM-1 (R241G (rs1799969) and K469E (rs5498)) gene polymorphisms was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results: According to results of our research, the A allele of the ICAM-1 R241G gene polymorphism appeared to be a risk factor for primary brain tumors (p<0.001). Similarly, the frequency of the A mutant allele of ICAM-1 R241G was statistically significant in patients with brain tumors classified as glioma (p<0.001). When allele and genotype distributions of ICAM-1 K469E, ICAM-1 R241G and ${\beta}3$ integrin Leu33Pro gene polymorphisms were evaluated with age, sex, and smoking, there were no statistically significant differences. Haplotype analysis revealed that the frequencies of GAC (rs1799969-rs5498-rs5918) and GAT (rs1799969-rs5498-rs5918) haplotypes were significantly lower in patients as compared with controls (p=0.001; p=0.036 respectively). Conclusions: This study provides the first evidence that ICAM-1 R241G SNP significantly contributes to the risk of primary brain tumors in a Turkish population. In addition, our results suggest that ICAM-1 R241G in combination ICAM-1 K469E may have protective effects against the development of brain cancer.

Granulocyte-colony stimulating factor decreases the extent of ovarian damage caused by cisplatin in an experimental rat model

Ali Akdemir,Burak Zeybek,Levent Akman,Ahment Mete Ergenoglu,Ahmet Ozgur Yeniel,Oytun Erbas,Altug Yavasoglu,Mustafa Cosan Terek,Dilek Taskiran 대한부인종양학회 2014 Journal of Gynecologic Oncology Vol.25 No.4

Objective: To investigate whether granulocyte-colony stimulating factor (G-CSF) can decrease the extent of ovarian follicle loss caused by cisplatin treatment. Methods: Twenty-one adult female Sprague-Dawley rats were used. Fourteen rats were administered 2 mg/kg/day cisplatin by intraperitoneal injection twice per week for five weeks (total of 20 mg/kg). Half of the rats (n=7) were treated with 1 mL/kg/day physiological saline, and the other half (n=7) were treated with 100 μg/kg/day G-CSF. The remaining rats (n=7, control group) received no therapy. The animals were then euthanized, and both ovaries were obtained from all animals, fixed in 10% formalin, and stored at 4oC for paraffin sectioning. Blood samples were collected by cardiac puncture and stored at -30oC for hormone assays. Results: All follicle counts (primordial, primary, secondary, and tertiary) and serum anti-Müllerian hormone levels were significantly increased in the cisplatin+G-CSF group compared to the cisplatin+physiological saline group. Conclusion: G-CSF was beneficial in decreasing the severity of follicle loss in an experimental rat model of cisplatin chemotherapy.

The Early Histological Effects of Intravesical Instillation of Platelet- Rich Plasma in Cystitis Models

M. İrfan Dönmez,Kubilay İnci,Naciye Dilara Zeybek,H. Serkan Doğan,Ali Ergen 대한배뇨장애요실금학회 2016 International Neurourology Journal Vol.20 No.3

Purpose: To evaluate the early histological effects of the intravesical instillation of platelet-rich plasma (PRP) in rabbit models of interstitial and hemorrhagic cystitis. Methods: Thirty-six rabbits were classified into 6 groups: saline (S), S+PRP, hydrochloric acid (HCl), HCl+PRP, cyclophosphamide (CyP), and CyP+PRP. At 48 hours after induction, PRP was prepared and intravesically administered to the S+PRP, HCl+PRP, and CyP+PRP groups. Bladder sections were stained with toluidine blue for mast cell counting and with hematoxylin and eosin for histopathology and mitotic index determination. The proliferation index was determined by proliferating cell nuclear antigen (PCNA) immunolabeling. The nonparametric Mann-Whitney U-test was used for statistical analysis. Results: No abnormalities were observed in the S group, whereas increased interstitial edema and increased average mitotic and proliferation indices were observed in the S+PRP group (P=0.023, P=0.004, and P=0.009, respectively). Intense epithelial loss, hemorrhage, and leukocyte infiltration were detected in the HCl and HCl+PRP groups, whereas a significantly increased average mitotic index was observed in the HCl+PRP group (P=0.002). When compared with its CyP counterpart, a significant reduction in hemorrhage and an increase in leukocyte infiltration and mitotic index were observed in the CyP+PRP group (P=0.006, P=0.038, and P=0.002, respectively). In addition, PCNA staining revealed a significantly increased proliferation index in the HCl+PRP and CyP+PRP groups (P=0.032 and P=0.015, respectively). Conclusions: The intravesical instillation of PRP increased the mitotic index in the saline and cyclophosphamide groups while decreasing macroscopic bleeding.

Thrombopoietin: a novel candidate tumor marker for the diagnosis of ovarian cancer

Timucin Mermer,Mustafa Cosan Terek,Burak Zeybek,Ahmet Mete Ergenoglu,Ahmet Ozgur Yeniel,Aydın Ozsaran,Osman Zekioglu 대한부인종양학회 2012 Journal of Gynecologic Oncology Vol.23 No.2

Objective: To investigate the decisive role of preoperative serum thrombopoietin levels in the discrimination of benign and malignant ovarian pathologies and its value in the evaluation of treatment response. Methods: Fifty patients with diagnoses of adnexal masses (25 benign, 25 malignant) were included in the study. Blood samples were collected from all cases preoperatively. Age, menopausal status, adnexal mass size, preoperative CA-125 level, platelet count, the stage of the disease (FIGO stage), tumor grade, histologic subgroup, the residual tumor mass, ascites cytology,surgical procedures, and postoperative treatments were recorded for the malignant group. Response to treatment was evaluated based on the revised RECIST guideline. Results: The preoperative serum thrombopoietin levels of the malignant cases (median, 98; range, 7 to 768) were significantly higher when compared with those of benign cases (median, 27; range, 13 to 131; p=0.004). The positive predictive value of CA-125 was found to be 79%, when it was used as a single marker; however it had risen to 85% when both CA-125 and thrombopoietin levels were used. There was no significant relationship between preoperative serum thrombopoietin levels and tumor grade, ascites cytology, presence of residual mass, and response to treatment. The preoperative serum thrombopoietin levels were significantly higher in stage III-IV cases and cases with serous histology. The post-treatment serum thrombopoietin levels in the malignant group were significantly lower as compared with the preoperative thrombopoietin levels. Conclusion: Thrombopoietin can play an additive role for prediction of ovarian cancer.