FSCB phosphorylation in mouse spermatozoa capacitation

( Shun Li Liu ),( Bing Ni ),( Xiang Wei Wang ),( Wen Qian Huo ),( Jun Zhang ),( Zhi Qiang Tian ),( Ze Min Huang ),( Yi Tian ),( Jun Tang ),( Yan Hua Zheng ),( Feng Shuo Jin ),( Yan Feng Li ) 생화학분자생물학회(구 한국생화학분자생물학회) 2011 BMB Reports Vol.44 No.8

It is generally accepted that spermatozoa capacitation is associated with protein kinase A-mediated tyrosine phosphorylation. In our previous study, we identified the fibrous sheath CABYR binding protein (FSCB), which was phosphorylated by PKA. However, the phosphorylation status of FSCB protein during spermatozoa capacitation should be further investigated. To this aim, in this study, we found that phosphorylation of this 270-kDa protein occurred as early as 1 min after mouse spermatozoa capacitation, which increased over time and remained stable after 60 min. Immunoprecipitation assays demonstrated that the tyrosine and Ser/Thr phosphorylation of FSCB occurred during spermatozoa capacitation. The extent of phosphorylation and was closely associated with the PKA activity and spermatozoa motility characteristics. FSCB phosphorylation could be induced by PKA agonist DB-cAMP, but was blocked by PKA antagonist H-89.Therefore, FSCB contributes to spermatozoa capacitation in a tyrosine-phosphorylated format, which may help in further elucidating the molecular mechanism of spermatozoa capacitation. [BMB reports 2011; 44(8): 541-546]

납두 혼합에 의한 개량식 된장 제조

민용규,김재욱 서울대학교농과대학농업개발연구소 1990 서울대농학연구지 Vol.15 No.2

된장의 품질을 향상시키기 위하여 3종류의 다른 된장 제조 시험을 실시하였다. 그 결과를 보면 다음과 같다. 환원당의 함량은 숙성 기간중 모든 시험구에 있어서 점차적으로 증가하였으며, 전질소의 함량도 약간 증가하는 경향을 나타내었다. 수용성 질소의 함량은 숙성 초기에는 급격히 증가하였으나 후기에 이르러 증가율이 둔화되었다. 아미노태 질소와 암모니아태 질소는 최대값을 가진 후에는 감소하는 경향을 보였다. pH는 전 숙성 기간중에 계속 감소하였다. 총산은 숙성 초기에는 급격히 증가하였으나, 후기에는 증가율이 완만하였다. 단백용해율과 단백분해율도 숙성초기에는 급격하게 증가하였으나 후기에는 감소하였다. 3개월 숙성된 된장으로 관능 검사를 실시한 결과, B구(대두 50, 보리 코오지 100, 납두 50)가 가장 좋았으며 A구(대두 100, 보리 코오지 100)가 C구(보리 코오지 100, 납두 100)보다 더 좋은 결과를 보였다. In order to improve the quality of Doenjang, three different types of Doenjang were made and tested. As the results, the amount of reducing sugar generally increased in all samples during aging. The content of total nitrogen increased a little during aging. The content of water soluble nitrogen increased rapidly during the initial period of aging, but the increase lagged thereafter. After amino nitrogen came to its peak during the aging period, it then began to come down. And so did the ammonia nitrogen. pH decreased through aging. Total acid increased rapidly in the initial period but it did not show much change in the following period. The ratio of protein solubility increased rapidly in the initial period of aging but the increase declined thereafter. The ratio of protein decomposition increased rapidly to the peak. In sensory evaluation of 3 months aged samples, sample B (soybean 50, barley koji 100, Natto 50) was the best and that sample A (soybean 100, barley koji 100) was better than sample C (barley koji 100, Natto 100).

들깨종자의 지방질 추출방법에 관한 연구

金載勖,全惠敬,閔龍圭 서울대학교 농과대학 농업개발연구소 1991 서울대농학연구지 Vol.16 No.2

Pearson법, Folch 법, Bligh 법, Pearson법 + Folch 법 및 Soxthlet 법 등 5가지 용매추출법을 수원 10호와 제주 들깨품종에 적용하여 용매추출방법 별 지방질 함량, 지방질 조성 및 지방산 조성을 분석한 결과 요약하면 다음과 같다. 1. 다섯가지 방법중 Soxhlet법에 의한 지방 추출량이 수원 10호와 제주의 경우 각각 43.3%, 25.2%로 가장 많았으며 Bligh법이나 Folch법과 같이 추출용매의 극성이 비교적 높은 방법은 들깨지방질 추출에는 비효율적이었다. 2. 들깨의 지방질에는 중성지방질이 95-99%로 가장 많이 함유되어 있으며 그중에서 트리글리세리드가 72-96%로 주종을 이루고 있다. 추출방법별로는 두 품종 다 같이 비교적 극성이 높은 추출용매를 이용한 Bligh법이나 Folch법에 의해 추출된 지방질에 극성지방질 획분이 많은 것으로 나타났다. 3. 수원 10호의 경우 추출방법에 의한 지방산 조성의 차이는 거의 없었으며 5가지 방법 모두 리놀렌산이 65%가량으로 주종을 이루고 있다. 추출방법별로는 두 품종 공히 비교적 극성이 높은 추출용매를 이용한 Bligh법이나 Folch법에 의해 추출된 지방질에 극성지방질 획분이 많은 것으로 나타났다. 3. 수원 10호의 경우 추출방법에 의한 지방산 조성의 차이는 거의 없었으며 5가지 방법 모두 리놀렌산 65%가량으로 주종을 이루고 있고 다불포화도는 7.8-10.6이었다. 제주의 경우, Bligh법은 다른 4가지 방법과는 달리 추출된 지방질의 지방산 조성에 있어서 리놀렌산이 38%, 올레산 29%, 팔미트산 17%, 리놀레산 12%로 다른 방법에 비해 리놀렌산의 함량이 적은 반면 올레산과 팔미트산의 함량이 많았다. Methods of perilla oil extraction Pearson's method, Folch's Method, Bligh's method, Soxhlet method, combination of Pearson's and Folch's method were compared about two variety of perilla seed, Suwon #10 and Jeju, Lipid composition and fatty acid composition were determined, As the results. Soxhlet method showed the highest extraction yield, 43.3% and 25.2% for Suwon #10 and Jeju, respectively, Bligh's and Folch methods which are effective in extraction of polar compounds were not effective in extraction of perilla oil. Neutral lipid which constitutes 95-99% of extracted oil, triglyceride constitutes 72-96% of neutral lipid, Bligh's method and Folch's method extracted more polar fraction than other methods. For the variety of Suwon #10, the five extraction methods showed similar composition of fatty acid. linolic acid was about 65% and degree of unsaturation was by 7.8-10.6, For the oil extracted by Bligh's method from Jeju variety showed the fatty acid composition as follows: linolenie acid 38%, oleic acid 29%, palmitic acid 17%. and linolic acid 17%, Bligh's method extracted more oleic and palmitic acid and less linolic acid than other methods.

Effects of different working fluids on the performance of a radial turbine in an organic Rankine cycle power system

Ze-min Bo,Zhenkun Sang,Xiaojing Lv,Yi-wu Weng 대한기계학회 2018 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.32 No.9

Organic Rankine cycle (ORC) power systems are widely used for low-temperature heat recovery. The radial turbine is the most important component in the ORC system and its performance is significantly dependent on the working fluid. Therefore, the effect of different working fluid on the performance of radial turbine in ORC system for the low-temperature heat source (120~180 °C) was investigated. First, the feasibilities of different working fluids for 150 kW radial turbine using R600a as designed working fluid were investigated. The thermal aerodynamic calculation of R600a at design condition was conducted with evaporation temperature 95 °C and condensation temperature 38 °C. The operation parameters of 5 non-designed working fluids (R600, R245fa, R245ca, R123 and R601a) were selected in the same temperature variation range as R600a. Then, the performance of a radial turbine for six working fluids at design condition was analyzed, and their three-dimensional flow field performances were also obtained by CFD numerical simulation. Finally, to broaden the operation range of the radial turbine and investigate the matching relationship between operation parameters and different working fluids, the off-design performances of radial turbine using six working fluids were analyzed and the effect of rotation speed, inlet pressure and inlet temperature on the performance of radial turbine was obtained. Results show that the performance of the radial turbine using the designed working fluid R600a is the best with efficiency of 82.7 % and output power of 150.5 kW. For the non-designed working fluids, the comprehensive performance of R600 was also judged to be good owing to the highest efficiency (85.1 %) and a relatively high power output (115.8 kW) among the fluids tested. For other working fluids, the output power of the radial turbine decreased obviously. There exists an optimum rotation speed for different working fluids to ensure that the radial turbine has the highest efficiency. The inlet pressure has a larger effect on the performance of the radial turbine than that of the inlet temperature. The results can provide basic data for the optimization design and operation of radial turbines in the ORC system.

Cross-immunizing potential of tumor MAGE-A epitopes recognized by HLA-A*02:01-restricted cytotoxic T Lymphocytes

( Ze Min Huang ),( Zheng Cai Jia ),( Jun Tang ),( Yi Zhang ),( Yi Tian ),( Dong Jing Ni ),( Fang Wang ),( Yu Zhang Wu ),( Bing Ni ) 생화학분자생물학회(구 한국생화학분자생물학회) 2012 BMB Reports Vol.45 No.7

Almost all melanoma cells express at least one member of the MAGE-A antigen family, making the cytotoxic T cells (CTLs) epitopes with cross-immunizing potential in this family attractive candidates for the broad spectrum of anti-melanoma immunotherapy. In this study, four highly homologous peptides (P264: FLWGPRALA, P264I9: FLWGPRALI, P264V9: FLWGPRALV, and P264H8: FLWGPRAHA) from the MAGE-A antigens were selected by homologous alignment. All four peptides showed high binding affinity and stability to HLA-A*02:01 molecules, and could prime CTL immune responses in human PBMCs and in HLA-A*02:01/Kb transgenic mice. CTLs elicited by the four epitope peptides could cross-lyse tumor cells expressing the mutual target antigens, except MAGE-A11 which was not tested. However, CTLs induced by P264V9 and P264I9 showed the strongest target cell lysis capabilities, suggesting both peptides may represent the common CTL epitopes shared by the eight MAGE-A antigens, which could induce more potent and broad-spectrum antitumor responses in immunotherapy. [BMB Reports 2012; 45(7): 408-413]

Difference from various finger skin conditions using surface friction experiment for the tactile sensitivity

Min-Seob Kim,Il-Young Kim,Se-Min Park,Yon-Kyu Park,Young-Ze Lee 한국트라이볼로지학회 2012 한국트라이볼로지학회 학술대회 Vol.2012 No.10

Identification of interacting proteins of retinoid-related orphan nuclear receptor gamma in HepG2 cells

( Ze Min Huang ),( Jun Wu ),( Zheng Cai Jia ),( Yi Tian ),( Jun Tang ),( Yan Tang ),( Ying Wang ),( Yu Zhang Wu ),( Bing Ni ) 생화학분자생물학회 (구 한국생화학분자생물학회) 2012 BMB Reports Vol.45 No.6

The retinoid-related orphan nuclear receptor gamma (RORγ) plays critical roles in regulation of development, immunity and metabolism. As transcription factor usually forms a protein complex to function, thus capturing and dissecting of the RORγ protein complex will be helpful for exploring the mechanisms underlying those functions. After construction of the recombinant tandem affinity purification (TAP) plasmid, pMSCVpuro RORγ-CTAP(SG), the nuclear localization of RORγ-CTAP(SG) fusion protein was verified. Following isolation of RORγ protein complex by TAP strategy, seven candidate interacting proteins were identified. Finally, the heat shock protein 90 (HSP90) and receptor-interacting protein 140 (RIP140) were confirmed to interplay with RORγ by co-immunoprecipitation. Interference of HSP90 or/and RIP140 genes resulted in dramatically decreased expression of CYP2C8 gene, the RORγ target gene. Data from this study demonstrate that HSP90 and RIP140 proteins interact with RORγ protein in a complex format and function as co-activators in the RORγ-mediated regulatory processes of HepG2 cells. [BMB Reports 2012; 45(6): 331-336]

Generation of Insulin-Producing Cells from PDX1 Gene-Modified Human Mesenchymal Stem Sells based on Lentiviral Vector System

( Min Jung Kim ),( Si Nae Kim ),( Ze Won Park ),( Hyung Min Chung ) 한국조직공학과 재생의학회 2008 조직공학과 재생의학 Vol.5 No.1

Mesenchymal stem cells(MSCs) are multipotent cells that may serve as a source of cells for generation of surrogate β cell. The objectives of this study are to generate insulin-producing cells in human mesenchymal stem cells(hMSCs) using PDX1(pancreatic duodenal homeobox 1) lentiviral vectors. PDX1 is essential for normal of pancreatic islet function as suggested by its regulatory action on the expression of a number of pancreatic genes, including insulin, somatostatin, islet amyoid polypeptide, glucagon. Thus, human mesenchymal stem cells(hMSCs) were exposed to a recombinant lentivirus expressing human PDX1 under control of EF-1a promoter. To induce a pancreatic fate in the PDX1 population, it was isolated by cell sorting, cultured in the presence of factors known to promote pancreatic development. Differentiated cells were analyzed by qRT-PCR and immunocytochemistry were further characterized for markers specific to mature pancreatic cells. In PDX1-expressing hMSCs, PAX4, Nkx6.1, insulin, glucagon mRNA levels increase significantly. Insulin-immunoreactivity was examined in PDX1-expressing hMSCs with DTZ-staining. In this study, we demonstrated that constitutive overexpression of exogenous PDX1 in hMSCs cells might have potentials to induce pancreatic lineage differentiation.

통합 Supply Chain Visibility를 고려한 최적화된 납기약속 Process의 접근방법 고찰 -A전자 사례를 중심으로-

함민제 ( Min Ze Ham ),이지훈 ( Ji Hoon Lee ) (주)엘지씨엔에스(구 LGCNS 엔트루정보기술연구소) 2012 Entrue Journal of Information Technology Vol.11 No.3

최근 하이테크 기업들의 글로벌화에 따른 공급사슬관리의 범위와 운영 복잡도의 증가는 고객 중심의 수요 지향적 비즈니스 환경으로의 전환에 따른 수요 변동성의 증가와 그 맥을 같이 하고 있다. 이에 따라 기업들은 제품의 가치를 통한 고객 만족뿐만 아니라 적시 납기 준수를 통한 고객 신뢰도 향상을 위한 공급사슬관리 역량 강화에 집중하고 있다. 본 연구에서는 납기약속 프레임워크의 고찰을 통해 납기약속 체계를 활용한 기업의 공급사슬관리 역량 향상과 운영적인 측면의 전략적 접근 방식을 제시한다. 그리고 MTS 기반 제조기업의 실제 구축 사례를 통해 거래선 대응 전략 및 시스템 기반 프로세스 운영 현황 등을 고려한 납기약속 체계의 단계적 접근 방법을 소개하여, 실제 기업 활용 측면의 운영 방법론을 제시한다. 본 연구의 접근 방법을 통해 기업은 외부적으로 고객 만족도 향상을 통한 서비스 수준을 제고하고, 내부적으로 실행 중심의 공급사슬관리 프로세스 개선을 통해 운영 역량을 강화할 수 있을 것으로 기대한다. Recently, the increase in scope and operational complexity of SCM due to globalization of the Hi-Tech Industry is highly related with demand volatility in the Customer-centric business environment. For this reason, many companies focus on SCM operational excellence in order to enhance customer satisfaction through both product leadership and on-time delivery. So this paper proposes a framework in which companies can accelerate the progress of SCM operation using Demand Fulfillment process. This study also introduces a case study on electronics industry and explains an effective operation methodology in MTS-based business operation. We expect that this study can provides the guideline with which company enhance customer service level and strengthen operation capability, both externally and internally.

해양환경에 따른 조력발전기 동기투입 지연현상 고찰

민제성(Ze-Sung Min),장태현(Tae-Hyeon Jang),김희곤(Hee-Gon Kim),곽원구(Won-Ku Kwak),손동수(Dong-Su Son) 대한전기학회 2019 대한전기학회 학술대회 논문집 Vol.2019 No.7