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      • KCI등재SCOPUS

        Effects of different working fluids on the performance of a radial turbine in an organic Rankine cycle power system

        Ze-min,Bo,Zhenkun,Sang,Xiaojing,Lv,Yi-wu,Weng 대한기계학회 2018 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.32 No.9

        Organic Rankine cycle (ORC) power systems are widely used for low-temperature heat recovery. The radial turbine is the most important component in the ORC system and its performance is significantly dependent on the working fluid. Therefore, the effect of different working fluid on the performance of radial turbine in ORC system for the low-temperature heat source (120~180 °C) was investigated. First, the feasibilities of different working fluids for 150 kW radial turbine using R600a as designed working fluid were investigated. The thermal aerodynamic calculation of R600a at design condition was conducted with evaporation temperature 95 °C and condensation temperature 38 °C. The operation parameters of 5 non-designed working fluids (R600, R245fa, R245ca, R123 and R601a) were selected in the same temperature variation range as R600a. Then, the performance of a radial turbine for six working fluids at design condition was analyzed, and their three-dimensional flow field performances were also obtained by CFD numerical simulation. Finally, to broaden the operation range of the radial turbine and investigate the matching relationship between operation parameters and different working fluids, the off-design performances of radial turbine using six working fluids were analyzed and the effect of rotation speed, inlet pressure and inlet temperature on the performance of radial turbine was obtained. Results show that the performance of the radial turbine using the designed working fluid R600a is the best with efficiency of 82.7 % and output power of 150.5 kW. For the non-designed working fluids, the comprehensive performance of R600 was also judged to be good owing to the highest efficiency (85.1 %) and a relatively high power output (115.8 kW) among the fluids tested. For other working fluids, the output power of the radial turbine decreased obviously. There exists an optimum rotation speed for different working fluids to ensure that the radial turbine has the highest efficiency. The inlet pressure has a larger effect on the performance of the radial turbine than that of the inlet temperature. The results can provide basic data for the optimization design and operation of radial turbines in the ORC system.

      • KCI등재SCIESCOPUS

        Generation of Insulin-Producing Cells from PDX1 Gene-Modified Human Mesenchymal Stem Sells based on Lentiviral Vector System

        ( Min Jung Kim ), ( Si Nae Kim ), ( Ze Won Park ), ( Hyung Min Chung ) 한국조직공학과 재생의학회 2008 조직공학과 재생의학 Vol.5 No.1

        Mesenchymal stem cells(MSCs) are multipotent cells that may serve as a source of cells for generation of surrogate β cell. The objectives of this study are to generate insulin-producing cells in human mesenchymal stem cells(hMSCs) using PDX1(pancreatic duodenal homeobox 1) lentiviral vectors. PDX1 is essential for normal of pancreatic islet function as suggested by its regulatory action on the expression of a number of pancreatic genes, including insulin, somatostatin, islet amyoid polypeptide, glucagon. Thus, human mesenchymal stem cells(hMSCs) were exposed to a recombinant lentivirus expressing human PDX1 under control of EF-1a promoter. To induce a pancreatic fate in the PDX1 population, it was isolated by cell sorting, cultured in the presence of factors known to promote pancreatic development. Differentiated cells were analyzed by qRT-PCR and immunocytochemistry were further characterized for markers specific to mature pancreatic cells. In PDX1-expressing hMSCs, PAX4, Nkx6.1, insulin, glucagon mRNA levels increase significantly. Insulin-immunoreactivity was examined in PDX1-expressing hMSCs with DTZ-staining. In this study, we demonstrated that constitutive overexpression of exogenous PDX1 in hMSCs cells might have potentials to induce pancreatic lineage differentiation.

      • KCI등재SCOPUS

        Identification of interacting proteins of retinoid-related orphan nuclear receptor gamma in HepG2 cells

        ( Ze Min Huang ), ( Jun Wu ), ( Zheng Cai Jia ), ( Yi Tian ), ( Jun Tang ), ( Yan Tang ), ( Ying Wang ), ( Yu Zhang Wu ), ( Bing Ni ) 생화학분자생물학회 (구 한국생화학분자생물학회) 2012 BMB Reports Vol.45 No.6

        The retinoid-related orphan nuclear receptor gamma (RORγ) plays critical roles in regulation of development, immunity and metabolism. As transcription factor usually forms a protein complex to function, thus capturing and dissecting of the RORγ protein complex will be helpful for exploring the mechanisms underlying those functions. After construction of the recombinant tandem affinity purification (TAP) plasmid, pMSCVpuro RORγ-CTAP(SG), the nuclear localization of RORγ-CTAP(SG) fusion protein was verified. Following isolation of RORγ protein complex by TAP strategy, seven candidate interacting proteins were identified. Finally, the heat shock protein 90 (HSP90) and receptor-interacting protein 140 (RIP140) were confirmed to interplay with RORγ by co-immunoprecipitation. Interference of HSP90 or/and RIP140 genes resulted in dramatically decreased expression of CYP2C8 gene, the RORγ target gene. Data from this study demonstrate that HSP90 and RIP140 proteins interact with RORγ protein in a complex format and function as co-activators in the RORγ-mediated regulatory processes of HepG2 cells. [BMB Reports 2012; 45(6): 331-336]

      • KCI등재SCOPUS

        Cross-immunizing potential of tumor MAGE-A epitopes recognized by HLA-A*02:01-restricted cytotoxic T Lymphocytes

        ( Ze Min Huang ), ( Zheng Cai Jia ), ( Jun Tang ), ( Yi Zhang ), ( Yi Tian ), ( Dong Jing Ni ), ( Fang Wang ), ( Yu Zhang Wu ), ( Bing Ni ) 생화학분자생물학회(구 한국생화학분자생물학회) 2012 BMB Reports Vol.45 No.7

        Almost all melanoma cells express at least one member of the MAGE-A antigen family, making the cytotoxic T cells (CTLs) epitopes with cross-immunizing potential in this family attractive candidates for the broad spectrum of anti-melanoma immunotherapy. In this study, four highly homologous peptides (P264: FLWGPRALA, P264I9: FLWGPRALI, P264V9: FLWGPRALV, and P264H8: FLWGPRAHA) from the MAGE-A antigens were selected by homologous alignment. All four peptides showed high binding affinity and stability to HLA-A*02:01 molecules, and could prime CTL immune responses in human PBMCs and in HLA-A*02:01/Kb transgenic mice. CTLs elicited by the four epitope peptides could cross-lyse tumor cells expressing the mutual target antigens, except MAGE-A11 which was not tested. However, CTLs induced by P264V9 and P264I9 showed the strongest target cell lysis capabilities, suggesting both peptides may represent the common CTL epitopes shared by the eight MAGE-A antigens, which could induce more potent and broad-spectrum antitumor responses in immunotherapy. [BMB Reports 2012; 45(7): 408-413]

      • KCI등재후보SCOPUS

        Molecular Cloning, Tissue Distribution and Segmental Ontogenetic Regulation of b0,+ Amino Acid Transporter in Lantang Pigs

        Ai-min,Zhi,Ding-yuan,Feng,Xiang-yan,Zhou,Shi-geng,Zou,Zhi-yi,Huang,Jian-jun,Zuo,Hui,Ye,Chang-ming,Zhang,Ze-min,Dong,Zhun,Liu 아세아·태평양축산학회 2008 Animal Bioscience Vol.21 No.8

        Cationic amino acid transporter b0,+AT (HGMW-approved gene symbol SLC7A9, solute carrier family 7, member 9) plays a crucial role in amino acid nutrition. In the present study, we describe the cloning and sequencing of porcine b0,+AT. Based on the sequence of porcine b0,+AT deposited in the NCBI (National Center for Biotechnological Information), we identified a putative porcine homologue. Using rapid amplification of cDNA ends (RACE), the full-length cDNA encoding porcine b0,+AT was isolated. The porcine b0,+AT cDNA was 1,680 bp long, encoding a 487 amino acid trans-membrane protein. The predicted amino acid sequence was found to have 88.9% and 87.1% identity with human and mouse b0,+AT, respectively. Real-time RT-PCR indicated porcine b0,+AT transcripts expressed in heart, kidney, muscle and small intestine. The small intestine had the highest b0,+AT mRNA abundance while the muscle had the lowest (p<0.05). Along the longitudinal axis, the ileum had the highest b0,+ AT mRNA abundance while the colon had the lowest (p<0.05). The b0,+AT mRNA level was highest on day 7 and 90 in the duodenum (p<0.05). It increased from day 1 to day 26 in the jejunum (p>0.05) and had the highest abundance on day 60 (p<0.05). There was, however, no difference between day 1, 7, 26, 30, 90 and 150 (p>0.05). The strongest b0,+AT expression appeared on day 7 in the ileum before weaning, and then decreased till day 30 but rose gradually again from day 60 to 150 (p<0.05).

      • KCI등재

        통합 Supply Chain Visibility를 고려한 최적화된 납기약속 Process의 접근방법 고찰 -A전자 사례를 중심으로-

        함민제 ( Min Ze Ham ), 이지훈 ( Ji Hoon Lee ) (주)엘지씨엔에스(구 LGCNS 엔트루정보기술연구소) 2012 Entrue Journal of Information Technology Vol.11 No.3

        최근 하이테크 기업들의 글로벌화에 따른 공급사슬관리의 범위와 운영 복잡도의 증가는 고객 중심의 수요 지향적 비즈니스 환경으로의 전환에 따른 수요 변동성의 증가와 그 맥을 같이 하고 있다. 이에 따라 기업들은 제품의 가치를 통한 고객 만족뿐만 아니라 적시 납기 준수를 통한 고객 신뢰도 향상을 위한 공급사슬관리 역량 강화에 집중하고 있다. 본 연구에서는 납기약속 프레임워크의 고찰을 통해 납기약속 체계를 활용한 기업의 공급사슬관리 역량 향상과 운영적인 측면의 전략적 접근 방식을 제시한다. 그리고 MTS 기반 제조기업의 실제 구축 사례를 통해 거래선 대응 전략 및 시스템 기반 프로세스 운영 현황 등을 고려한 납기약속 체계의 단계적 접근 방법을 소개하여, 실제 기업 활용 측면의 운영 방법론을 제시한다. 본 연구의 접근 방법을 통해 기업은 외부적으로 고객 만족도 향상을 통한 서비스 수준을 제고하고, 내부적으로 실행 중심의 공급사슬관리 프로세스 개선을 통해 운영 역량을 강화할 수 있을 것으로 기대한다. Recently, the increase in scope and operational complexity of SCM due to globalization of the Hi-Tech Industry is highly related with demand volatility in the Customer-centric business environment. For this reason, many companies focus on SCM operational excellence in order to enhance customer satisfaction through both product leadership and on-time delivery. So this paper proposes a framework in which companies can accelerate the progress of SCM operation using Demand Fulfillment process. This study also introduces a case study on electronics industry and explains an effective operation methodology in MTS-based business operation. We expect that this study can provides the guideline with which company enhance customer service level and strengthen operation capability, both externally and internally.

      • SCIESCOPUSKCI등재후보

        Cross-immunizing potential of tumor MAGE-A epitopes recognized by HLA-A<sup>*</sup>02:01-restricted cytotoxic T lymphocytes

        Huang,,Ze-Min,Jia,,Zheng-Cai,Tang,,Jun,Zhang,,Yi,Tian,,Yi,Ni,,Dong-Jing,Wang,,Fang,Wu,,Yu-Zhang,Ni,,Bing Korean Society for Biochemistry and Molecular Biol 2012 BMB Reports Vol.45 No.7

        Almost all melanoma cells express at least one member of the MAGE-A antigen family, making the cytotoxic T cells (CTLs) epitopes with cross-immunizing potential in this family attractive candidates for the broad spectrum of anti-melanoma immunotherapy. In this study, four highly homologous peptides (P264: FLWGPRALA, P264I9: FLWGPRALI, P264V9: FLWGPRALV, and P264H8: FLWGPRAHA) from the MAGE-A antigens were selected by homologous alignment. All four peptides showed high binding affinity and stability to HLA-A$^*02:01$ molecules, and could prime CTL immune responses in human PBMCs and in HLA-A$^*02:01/K^b$ transgenic mice. CTLs elicited by the four epitope peptides could cross-lyse tumor cells expressing the mutual target antigens, except MAGE-A11 which was not tested. However, CTLs induced by P264V9 and P264I9 showed the strongest target cell lysis capabilities, suggesting both peptides may represent the common CTL epitopes shared by the eight MAGE-A antigens, which could induce more potent and broad-spectrum antitumor responses in immunotherapy.

      • SCIESCOPUSKCI등재후보

        Identification of interacting proteins of retinoid-related orphan nuclear receptor gamma in HepG2 cells

        Huang,,Ze-Min,Wu,,Jun,Jia,,Zheng-Cai,Tian,,Yi,Tang,,Jun,Tang,,Yan,Wang,,Ying,Wu,,Yu-Zhang,Ni,,Bing Korean Society for Biochemistry and Molecular Biol 2012 BMB Reports Vol.45 No.6

        The retinoid-related orphan nuclear receptor gamma ($ROR{\gamma}$) plays critical roles in regulation of development, immunity and metabolism. As transcription factor usually forms a protein complex to function, thus capturing and dissecting of the $ROR{\gamma}$ protein complex will be helpful for exploring the mechanisms underlying those functions. After construction of the recombinant tandem affinity purification (TAP) plasmid, pMSCVpuro $ROR{\gamma}$-CTAP(SG), the nuclear localization of $ROR{\gamma}$-CTAP(SG) fusion protein was verified. Following isolation of $ROR{\gamma}$ protein complex by TAP strategy, seven candidate interacting proteins were identified. Finally, the heat shock protein 90 (HSP90) and receptor-interacting protein 140 (RIP140) were confirmed to interplay with $ROR{\gamma}$ by co-immunoprecipitation. Interference of HSP90 or/and RIP140 genes resulted in dramatically decreased expression of CYP2C8 gene, the $ROR{\gamma}$ target gene. Data from this study demonstrate that HSP90 and RIP140 proteins interact with $ROR{\gamma}$ protein in a complex format and function as co-activators in the $ROR{\gamma}$-mediated regulatory processes of HepG2 cells.

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