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Xing-Xi Gao,Jun-Hua Wu,Da-Hua Shi,Yun-Xi Chen,Jiang-Tao Cui,Yu-Rong Wang,Chun-Ping Jiang 대한약학회 2012 Archives of Pharmacal Research Vol.35 No.8
The aim of this study was to investigate the effects of tectorigenin on chemically induced liver fibrosis in rats. Liver fibrosis was induced in rats with carbon tetrachloride, a diet high in fat, cholesterol and alcohol in the drinking water. Our results indicate that tectorigenin treatment significantly inhibited the increases in the activities of alanine aminotransferase (ALT),aspartate aminotransferase (AST) and the increases in the serum levels of hyaluronate (HA), laminin (LN) and procollagen III N-terminal peptide (PIIIP); tectorigenin treatment also significantly inhibited the increases in the amount of collagen in the livers of the fibrogenic rats. Chemically induced liver fibrosis caused a drop in the serum albumin concentration and a decrease in the ratio of albumin to globulin (A/G). Tectorigenin caused a remarkable increase at a dose of 30 mg/kg, but only a slight increase at the lower doses. Tectorigenin was also able to inhibit the increase in the liver lipid peroxidation (LPO), as well as the decrease in the activities of liver superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), caused by liver fibrosis. In addition, we present a related metabolic profile determined, using a 1H NMR spectroscopy and multivariate pattern recognition techniques. The results were consistent with the pathological examination, liver function analysis and liver fibrosis marker analysis. Furthermore, tectorigenin does not cause acute toxicity.
Xi-yue Liu,Yuan-Qing Wang,Liang Zong,Yun Lin,Yong-Jiu Shi 한국강구조학회 2014 International Journal of Steel Structures Vol.14 No.3
Compared with the conventional steel structure, the high-strength steel structures are at more risk of brittle fracture, especiallyin cold regions. In the present study, a series of tests (such as uniaxial tensile test, Charpy impact test and three-point bendingtest) were carried out at low temperature to investigate the mechanical properties and toughness of Q460C steel and its buttwelded joint, fracture micro-mechanisms were analyzed as well. The ductility indices and the toughness indices all decreasewith temperature decreases, the heat affected zone (HAZ) in welded joint is more critical to fracture than the base material. The fracture toughness of high-strength steel Q460C is relatively lower than the other three conventional steels (i.e. Q235, Q345and Q390). In this study, rich experimental data were collected so as to provide reference for the fracture resistant design ofhigh-strength steel structures in cold regions.
Mesenchymal Stem Cells on a Decellularized Cartilage Matrix for Cartilage Tissue Engineering
Xi-Fu Zheng,Shi-Bi Lu,Wei-Guo Zhang,Shu-Yun Liu,Jing-Xiang Huang,Quan-Yi Guo 한국생물공학회 2011 Biotechnology and Bioprocess Engineering Vol.16 No.3
An ideal scaffold for cartilage tissue engineering should be biomimetic in not only its biochemical composition, but also in the morphological structure of the scaffold. In this study, we fabricated a scaffold with an oriented structure using a nanofibrous articular cartilage extracellular matrix (ACECM), in which the ACECM was used to mimic the biochemical composition and oriented structure of articular cartilage. Histology analysis showed that the scaffold contained cartilage ECM (GAGs and collagen II). Scanning electron microscopy (SEM) indicated that the scaffolds were composed of nanofibers and possessed vertical microtubules. Chondrogenic differentiation-induced mesenchymal stem cells (MSCs) were seeded on the scaffold in vitro. SEM showed that MSCs proliferated well and aligned along the vertical microtubules,which mimicked the orientation of deep zone articular cartilage. A cell proliferation assay and live/dead cell staining demonstrated that the ACECM possessed good cell affinity, which favored cell adherence and proliferation. The MSCs that had been labeled with the fluorescent dye PKH26 and seeded on scaffolds were implanted into nude mice. The differentiated cells/ACECM implants formed cartilage-like tissue 4 weeks after implantation, and stained positive for collagen type II and toluidine blue. In addition,the in vivo fluorescent images verified that the MSCs in the implants were the labeled MSCs. These results demonstrated that the oriented ACECM scaffolds hold great promise for use in cartilage tissue engineering applications.
Xi-xiang Ying,Hai-bo Li,Zheng-yun Chu,Yan-jun Zhai,Ai-jing Leng,Xun Liu,Wen-jie Zhang,Ting-guo Kang,Chun Xin 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.7
To investigate the antioxidant effect of vitexin-4"-O-glucoside, a flavone glycoside, isolated from the leaves of Crataegus pinnatifida Bge. var. major, we developed a simple and sensitive high-performance liquid chromatography (HPLC) method to determine levels of malondialdehyde (MDA) in ECV304 cell culture medium after induction by tert-butyl-hydroperoxide (TBHP). The preparation of analyzed samples involved a one-step derivatization with thiobarbituric acid (TBA). HPLC analysis was performed on a SynergiTM Hydro-RP, a polar end-capped C18 column (250×4.6 mm, 4 μm), using an acetonitrile-ammonium acetate aqueous solution (10 mM, pH 6.8) as the mobile phase under linear gradient conditions with UV detection at 532 nm. The calibration curve was linear over 0.0125-1.25 μM MDA (r = 0.9951). Relative standard deviations (RSDs) of intra-day and inter-day precision were less than 6.1% and 5.0%, respectively. The mean recovery was 96.9 ± 1.6%. The lower limit of quantification (LLOQ) of MDA was 0.0125 μM. This chromatographic method was successfully applied to investigating the in vitro antioxidant effect of vitexin-4"-O-glucoside. Vitexin-4"-O-glucoside (120 M) protected ECV304 cells from peroxidation induced by TBHP.
The role of WRKY47 gene in regulating selenium tolerance in Arabidopsis thaliana
Xi Wu,Manzhi Tao,Yun Meng,Xiangyu Zhu,Liangwen Qian,Alia Shah,Wei Wang,Shuqing Cao 한국식물생명공학회 2020 Plant biotechnology reports Vol.14 No.1
Selenium stress has a serious impact on the growth of plants. However, the available understanding on the mechanism of Se stress response is still insufficient. In this study, we found that the WRKY47 gene plays an important role in maintaining Se homeostasis and tolerance in Arabidopsis thaliana. Expression of WRKY47 is induced by Se stress. The wrky47 mutants are sensitive to Se stress, and this sensitivity is associated with a decrease in the transcription levels of Se-detoxify genes HMT1 and HMT3. Surprisingly, the WRKY47-overexpression lines also showed increased sensitivity to Se stress and had increased Se content; this correlates to an increase in the transcription level of Se-uptake gene PHT1;4 but not Se-detoxify genes HMT1 and HMT3. Our results suggest that gain- and loss-of-function mutations in WRKY47 enhance the sensitivity of A. thaliana plants to Se stress through different mechanisms.
Soy Protein Supplementation Reduces Clinical Indices in Type 2 Diabetes and Metabolic Syndrome
Xi-Mei Zhang,Yun-Bo Zhang,Mei-Hua Chi 연세대학교의과대학 2016 Yonsei medical journal Vol.57 No.3
Purpose: Clinical trials have studied the use of soy protein for treating type 2 diabetes (T2D) and metabolic syndrome (MS). The purpose of this study was to outline evidence on the effects of soy protein supplementation on clinical indices in T2D and MS subjects by performing a meta-analysis of randomized controlled trials (RCTs). Materials and Methods: We searched PubMed, EMBASE, and Cochrane databases up to March 2015 for RCTs. Pooled estimates and 95% confidence intervals (CIs) were calculated by the fixed-and-random-effects model. A total of eleven studies with eleven clinical variables met the inclusion criteria. Results: The meta-analysis showed that fasting plasma glucose (FPG) [weighted mean difference (WMD), -0.207; 95% CI, -0.374 to -0.040; p=0.015], fasting serum insulin (FSI) (WMD, -0.292; 95% CI, -0.496 to -0.088; p=0.005), homeostasis model of assessmentfor insulin resistance index (HOMA-IR) (WMD, -0.346; 95% CI, -0.570 to -0.123; p=0.002), diastolic blood pressure (DBP) (WMD, -0.230; 95% CI, -0.441 to -0.019; p=0.033), low-density lipoprotein cholesterol (LDL-C) (WMD, -0.304; 95% CI, -0.461 to -0.148; p=0.000), total cholesterol (TC) (WMD, -0.386; 95% CI, -0.548 to -0.225; p=0.000), and C-reactive protein (CRP) (WMD, -0.510; 95% CI, -0.722 to -0.299; p=0.000) are significant reduced with soy protein supplementation, compared with a placebo control group, in T2D and MS patients. Furthermore, soy protein supplementation for longer duration (≥6 mo) significantly reducedFPG, LDL-C, and CRP, while that for a shorter duration (<6 mo) significantly reduced FSI and HOMA-IR. Conclusion: Soy protein supplementation could be beneficial for FPG, FSI, HOMA-IR, DBP, LDL-C, TC, and CRP control in plasma.
SPEAKER IDENTIFICATION EXPERIMENT USING KOHONEN`S FEATURE MAP NEURAL NETWORK
Yun, Li Qing,Yao, Li Xue,Hong, Wu Xi,Hong, Liu 대한전자공학회 1992 HICEC:Harbin International Conference on Electroni Vol.1 No.1
Text-independent speaker identification using Kohonen's self-organizing feature map as a quantizer is described in this paper. We proposed a new idea that each neural network is created for each speaker. The recognition features are 12 order LPC cepstrum coefficients. A recognition rate (average accuracy) of 92% is reached in this experiment.