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Yukihiro Asami,김순옥,장준필,고성균,김보연,Hiroyuki Osada,장재혁,안종석 한국미생물·생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.1
We previously identified a new heparinase inhibitor fungal metabolite, named CRM646-A, which showed inhibition of heparinase and telomerase activities in an in vitro enzyme assay and antimetastatic activity in a cell-based assay. In this study, we elucidated the mechanism by which CRM646-A rapidly induced nucleus condensation, plasma membrane disruption and morphological changes by increasing intracellular Ca2+ levels. Furthermore, PD98059, a mitogen-activated protein kinase (MEK) inhibitor, inhibited CRM646-A-induced nucleus condensation through ERK1/2 activation in rat 3Y1 fibroblast cells. We identified CRM646-A as a Ca2+ ionophore-like agent with a distinctly different chemical structure from that of previously reported Ca2+ ionophores. These results indicate that CRM646-A has the potential to be used as a new and effective antimetastatic drug.
Yukihiro Kubota,Yuto Ohnishi,Tasuku Hamasaki,Gen Yasui,Natsumi Ota,Hiromu Kitagawa,Arashi Esaki,Muhamad Fahmi,Masahiro Ito 한국유전학회 2021 Genes & Genomics Vol.43 No.5
Background Histone deacetylase (HDAC)-1, a Class-I HDAC family member, forms three types of complexes, the nucleosome remodeling deacetylase, Sin3, and CoREST complexes with the specifc corepressor components chromodomainhelicase-DNA-binding protein 3 (Mi2/CHD-3), Sin3, and REST corepressor 1 (RCOR1), respectively, in humans. Objective To elucidate the functional relationships among the three transcriptional corepressors during embryogenesis. Methods The activities of HDA-1, LET-418, SIN-3, and SPR-1, the homologs of HDAC-1, Mi2, Sin3, and RCOR1 in Caenorhabditis elegans during embryogenesis were investigated through measurement of relative mRNA expression levels and embryonic lethality given either gene knockdown or deletion. Additionally, the terminal phenotypes of each knockdown and mutant embryo were observed using a diferential-interference contrast microscope. Finally, the functional relationships among the three corepressors were examined through genetic interactions and transcriptome analyses. Results Here, we report that each of the corepressors LET-418, SIN-3, and SPR-1 are expressed and have essential roles in C. elegans embryonic development. Our terminal phenotype observations of single mutants further implied that LET-418, SIN-3, and SPR-1 play similar roles in promoting advancement to the middle and late embryonic stages. Combined analysis of genetic interactions and gene ontology of these corepressors indicate a prominent overlapping role among SIN-3, SPR-1, and LET-418 and between SIN-3 and SPR-1. Conclusion Our fndings suggest that the class-I HDAC-1 corepressors LET-418, SIN-3, and SPR-1 may cooperatively regulate the expression levels of some genes during C. elegans embryogenesis or may have some similar roles but functioning independently within a specifc cell.
Implementation of Optical Flow Measurement System with an Embedded Processor
Yukihiro Sugiki,Teruo Yamaguchi,Hiroshi Harada 제어로봇시스템학회 2015 제어로봇시스템학회 국제학술대회 논문집 Vol.2015 No.10
Optical flow estimation is one of the measuring method of object motion. It is important to prove that optical flow measurement system can perform in small scale computer to use as visual sensor. In this research, processing speed of optical flow measurement system with a single board computer Raspberry Pi was evaluated. Calclation time of optical flow estimation program based on spatio-temporal differentiation method with eigenvalue decomposition was compared with those using built-in optical flow function in OpenCV library (Lucas-Kanade method and Horn-Schunck method). As a result, the program was about the same processing speed as HS method, and took about six times as long as LK method. It is also shown when an object moves at a velocity of about 10 pixels per frame or more, output results apt to show wrong velocity vectors. Processing speed is to be improved by selecting optimum pixels required for velocity estimation. It will be necessary to compensate for velocity so that it is able to estimate optical flow at a high speed.
( Yukihiro Yano ),( Hiroyuki Kagawa ),( Seigo Kitada ),( Masahide Mori ),( Soichiro Yokota ),( Ryoji Maekura ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1
Background: To date, the clinical signifi cance of Mycobacterium (M.) gordonae isolated from respiratory specimens has not been well investigated. This study aimed to determine the clinical signifi cance of M. gordonae isolated from respiratory specimens and the validity of the diagnostic guideline for pulmonary nontuberculous mycobacterial (NTM) disease as applied patients with M. gordonae. Methods: A retrospective observational study was conducted on all patients from whom M. gordonae was isolated between 2003 and 2013 in our institution, which is a specialist hospital for respiratory disease in Osaka, Japan. M. gordonae was identifi ed by the DNA-DNA hybridization method. We identifi ed patients who met diagnostic criteria according to the guideline of the American Thoracic Society (ATS) for pulmonary NTM disease and confi rmed fi nal defi nitive diagnosis of such patients. Results: M. gordonae isolates were found in respiratory specimens from 152 patients who were examined multiple times. A total of 10 patients met the diagnostic criteria based on pulmonary symptoms, abnormal radiographic fi ndings, and multiple identifi cations of M. gordonae from respiratory specimens. Among these 10 patients, only 2 were confi rmed as having pulmonary disease caused by M. gordonae. Colonization or contamination was diagnosed in the remaining 150 patients. The ratio of patients confi rmed as having pulmonary disease caused by M. gordonae to those from whom M. gordonae was isolated, was 2/152 (1.32%). The positive predictive value of the ATS guideline for pulmonary NTM disease when applied to patients with M. gordonae was 20% (2/10 patients). Conclusions: Occurrence of pulmonary disease caused by M. gordonae was rare and the present ATS guideline for pulmonary NTM disease may be inappropriate when applied to patients with M. gordonae.