Blocking of Cell Proliferation, Cytokines Production and Genes Expression Following Administration of Cordyceps Sinensis in the Bronchoalveolar Lavage Fluids Cells

Kuo, Yuh-Chi,Wu, Chia-Lin,Tasi, Wei-Jern,Wang, Jir-Yenn,Chang, Shi-Chung,Lin, Ching-Yuang,Shiao, Ming-Shi 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Yuh-Chi Kuo¹,Chia-Lin Wn¹,Wei-jern Tasi¹,Jir-Yenn Wang²,Shi-Chung Chang₃,Ching-Yung Lin⁴,and Ming-Shi Shiao⁴¹National Research Institute of Chinese Medicine, ²Department of Biology,Fu Jen University, ³Department of Chest, ⁴Department of Pediatrics, □De[art,amt of Medical Research and Education. Taipei Veterans General Hospital, Taipei, Taiwan Yuh-Chi Kuo¹,Chia-Lin Wn¹,Wei-jern Tasi¹,Jir-Yenn Wang²,Shi-Chung Chang²,Ching-Yung Lin³,and Ming-Shi Shiao⁴.¹National Research Institute of Chinese Medicine, ²Department of Biology,Fu Jen University, ³Department of Chest, ⁴Department of Pediatrics, □De[art,amt of Medical Research and Education. Taipei Veterans General Hospital, Taipei, Taiwan. Blocking of Cell Proliferation, Cytokines Production and Genes Expression Following Administration of Cordyceps sinensis in the Bronchoalveolar Lavage Fluids Cells. Proceedings of international Symposium on East-West Medicine, Seoul. 44-64, 1999.-Cordyceps sinensis is a major parasitic fungus on larva of Lepidoptera. It is one of the well know fungi used in traditional Chinese medicine for treatment asthma, lung and kideney diseases. Many fungi belonging to the genus Cordyceps have been demonstrated to produce natural products with various biological activities. Reported biological activities of genus cordyceps include:(a)inhibition of DNA and RNA synthesis;(b)enhancement of cell differentiation;(c)restruction of cytoskeleton;(d)inhibition of protein kinase activity;(e)antitumor activity on bladder, colon, lung carcinoma as well as fibroblastoma;(f)inhibition of the infection and revers transciptase activity of human immunodeficiency virus type 1; and(g) inhibition of methylation of nucleic acid. Thus, it is suggested that the extracts of Cordyceps sp. Contain antitumor, antiviral, and many other biological activities. In our previous studies, we proved that C. sinensis contains the antitumor and immunomodulatory agents. In the present studies, effects of C. sinensis on bronchoalveolar lavage fluids(BAL)cells were demonstrated. The crude methanolic extracts of C. sinensis were fractionated into 15 fractions by silica gel column chromatography. Effects of various fraction on BAL cells proliferation and lipopolysaccharide(LPS) stimulated interleukin-1β(IL-8),platelet activating factor(PAF), interleukin-6(IL-6), tumor necrosis factor-α(TNF-α),and interleukin-8(IL-8) production on BAL cells were determined. The result indicated that the CS-19-22 fraction suppressed BAL cells proliferation activated by LPS. The median inhibitory concentration(IC50) was 6㎍/ml. The CS-19-22 fraction also decreased IL-1β,PAF,IL-6, TNF-αand IL-8 production.The results of revers transcription-polymerase chain reaction(RT-PCR) demonstrated that the CS-19-22 fraction did not affect IL-1β,PAF,IL-6, TNF-α,and IL-8 mRNAs expression in BAL cells activated by LPS. By contrast, it inhibited the IL-10 mRNA expression but enhanced IFN-γand IL-12 mRNAs expression in activated BAL cells. Moreover, the CS-19-22 fraction blocked PAF-induced platelet aggregation. It is unlikely that cytotoxicity was involved. Because no cell deths were observable. We hypothesize that inhibitory mechanisms of CS-19-22 on BAL cells proliferation may be related to the impairments of gene expression and production of cytokines in BAL cells. The explanation of C. sinensis antiasthma function may involved the following(1)C.sinenesis contained immunomodulatory agents that stimulated IFN-γand IL-12 expression in TH1cells.(2)Enhanced secretion of IFN-γand IL-12 will inhibit the Tγcells immune responses Ex. Decreasing of IL-6 and IL-10 production.(3) The impairments of cytokines production in TH2 cells suppressed th BAL cells proliferation then more cytokines production Ex.IL-1β,PAF,IL-6,TNF-αand IL-8 in activated BAL cells were decreased. (4)The suppression of TH2cells immune responses will inhibit the growth and differentiation of B cells then IgE production will be decreased. (5)Reduced production of IgE will decreased asthma attack occurring in individuals. However, the results of the present study suggested that C. sinensis may also have acted to treat asthma in part by inhibiting BAL cells proliferation and cytokine gene expression and production. Future, experiments with treatment of animals with asthma with C. sinensis will be necessary to defined whether C. sinensis can reduce asthma injury. Plans are underway for the isolation of pure principal growth inhibitors and their mechanisms of action are subjected for further study.

A new dual fluorogenic and chromogenic ''turn-on'' chemosensor for Cu²⁺/F^- ions

Yu, H.,Lee, J.Y.,Angupillai, S.,Wang, S.,Feng, S.,Matsumoto, S.,Son, Y.A. Pergamon 2015 Spectrochimica acta. Part A, Molecular and biomole Vol.151 No.-

<P>Turn 'off-on' chemosensor 2-(-2-((3',6'-bis(diethylamino)-3-oxospiro[isoindoline-1,9'-xanthen]-2-yl)imino)ethylidene)-N-phenylhydrazine-1-carbothioamide (RBS) was designed and synthesized. Using the naked eye, RBS showed favorable observation characteristics with both Cu2+ and F- ions. The various modes of sensitivity shown by RBS toward the Cu2+ and F- ions were investigated by spectral techniques, including UV-Vis, fluorescence and H-1 NMR spectroscopy. The Job's plot indicated the formation of 1:1 complex between RBS and Cu2+/F-. The binding constant of the RBS-guest(-) complexes were found to be 1.3 x 10(4) and 6.2 x 10(3) M-1 for the RBS-Cu2+ and RBS-F-, respectively. (C) 2015 Elsevier B.V. All rights reserved.</P>

Fourier-transform light scattering of individual colloidal clusters

Yu, H.,Park, H.,Kim, Y.,Kim, M.W.,Park, Y. OPTICAL SOCIETY OF AMERICA 2012 Optics letters Vol.37 No.13

Recent advances in wavefront shaping techniques for biomedical applications

Yu, H.,Park, J.,Lee, K.,Yoon, J.,Kim, K.,Lee, S.,Park, Y. Elsevier 2015 CURRENT APPLIED PHYSICS Vol.15 No.5

Due to the highly inhomogeneous distributions of refractive indexes, light propagation in complex media such as biological tissue experiences multiple light scattering events. The suppression and control of multiple light scattering events are investigated because they offer the possibility of optical focusing and imaging through biological tissues, and they may open new avenues for diagnosis and treatment of several human diseases. In order to provide insight into how new optical techniques can address the issues of multiple light scattering in biomedical applications, the recent progress in optical wavefront-shaping techniques is summarized.

Relative quantitation of restriction fragment length polymorphic DNAs via DNA melting analysis provides an effective way to determine allele frequencies

Yu, H.,Koo, I.,Jeong, S. Academic Press 2009 Genomics Vol.94 No.5

To accurately and precisely estimate the allele frequencies in DNA pools for a cost-effective approach to correlate genetic variations to phenotypic traits, we exploited differential melting kinetics between restriction fragment length polymorphic DNAs. The allele frequencies of two SNPs in a series of DNA mixtures with known allelic compositions of the SNPs were determined by analyzing the meltings of restricted PCR amplicons, yielding a result with a root mean square error (RMSE) of 0.014 relative to the expected values and a standard deviation (SD) of 0.018 from triplicate measurements. This method was then applied in the measurement of genotype frequencies in DNA pools in which varying numbers of genomic DNAs were intermingled while maintaining uniform quantitative contribution. Analyses of 10 SNPs demonstrated the feasibility of this method in an economical and highly accurate manner as the results yielded an RMSE value of 0.027 and a SD of 0.019.

Effects of extrusion speed on the microstructure and mechanical properties of ZK60 alloys with and without 1wt% cerium addition

Yu, H.,Hyuk Park, S.,Sun You, B.,Min Kim, Y.,Shun Yu, H.,Soo Park, S. Elsevier Sequoia 2013 Materials science & engineering. properties, micro Vol.583 No.-

The effects of extrusion speed on the microstructure and tensile properties of the ZK60 and ZK60-1Ce alloys were investigated by performing indirect extrusion at three ram speeds (0.3, 1.0 and 3.0mm/s). All of the extruded alloys showed a bimodal microstructure consisting of equiaxed fine recrystallized (DRXed) grains and elongated coarse unDRXed grains. With increasing extrusion speed, the exit temperature increased due to deformation heating, resulting in a larger grain and a higher DRXed fraction. The yield and ultimate tensile strengths and elongation at RT decreased with an increase of extrusion speed. The ZK60-1Ce alloys exhibited a finer grain size, a higher DRXed fraction, and weaker texture intensity than the ZK60 alloys at the same extrusion speed due to the inhibition of grain growth by the pinning effect and the promotion of DRX by particle-stimulated nucleation. The yield and ultimate tensile strengths at room and elevated temperatures were increased by the addition of Ce, while elongation was decreased due to cracking at the Mg-Zn-Ce particles.

Porous-layered stack of functionalized AuNP-rGO (gold nanoparticles-reduced graphene oxide) nanosheets as a sensing material for the micro-gravimetric detection of chemical vapor

Yu, H.,Xu, P.,Lee, D. W.,Li, X. Royal Society of Chemistry 2013 Journal of materials chemistry. A, Materials for e Vol.1 No.14

Development of extraordinary high-strength Mg-8Al-0.5Zn alloy via a low temperature and slow speed extrusion

Yu, H.,Park, S.H.,You, B.S. Elsevier Sequoia 2014 Materials science & engineering. properties, micro Vol.610 No.-

An extraordinary high strength Mg-8Al-0.5Zn alloy has been developed through extrusion at a low temperature of 200<SUP>o</SUP>C and a slow speed of 0.07mm/s. The as-extruded alloy exhibited a tensile yield strength of 403MPa, an ultimate tensile strength of 437MPa, an elongation of 10.7%, and a low tension-compression yield asymmetry of 0.97. These superior mechanical properties are attributed to the combined effect of ultra-fine recrystallized grains, numerous nano-scale Mg<SUB>17</SUB>Al<SUB>12</SUB> precipitates, and strong basal texture.

Die angle dependency of microstructural inhomogeneity in an indirect-extruded AZ31 magnesium alloy

Yu, H.,Park, S.H.,You, B.S. Elsevier 2015 Journal of materials processing technology Vol.224 No.-

The variation induced in the microstructural inhomogeneity of AZ31 alloy subjected to indirect extrusion was studied using tool steel dies with angles of 30<SUP>o</SUP>, 60<SUP>o</SUP> and 90<SUP>o</SUP>. Finite element modeling (FEM) of this extrusion process was also carried out to determine the metal flow, temperature evolution and effective strain distribution, which were then correlated with the observed microstructural changes. Although all extruded samples were found to have a bimodal microstructure consisting of equiaxed fine recrystallized (DRXed) grains and elongated coarse unDRXed grains, the inhomogeneity of their microstructural characteristics (i.e., DRX fraction and texture intensity) decreased in cross section with an increase in the die angle. The FEM analysis also demonstrated that a faster metal flow, higher temperature, and larger effective strain are generated in an alloy extruded with a 90<SUP>o</SUP> die angle, and that this enhances DRX behavior during extrusion and ultimately results in a homogeneously DRXed microstructure.

Study on Improving the Degree of Size Precision of Rack Tube

Yu, H.,Kim, D.H.,Jung, D.W. TRANS TECH PUBLICATION LTD 2014 Advanced materials research Vol.1016 No.-