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안구적출에 따른 위둔덕의 칼슘결합단백질의 재구축 및 상호 연관성
안병수,고길석,안명수,김경주,권안성,정명섭,박춘매,조병옥,김진우,Samudra Acharya,Parmeshwar Narayan Amatya,장인엽 朝鮮大學校 附設 醫學硏究所 2007 The Medical Journal of Chosun University Vol.32 No.1
Background: Superior colliculus is a part of midbrain, and participates in the visual reflexes, It receives afferent fibers from optic nerve, visual cortex, and spinotectal tract. After optic deprivation, the microscopic structure of the superior colliculus changed. Calcium-binding proteins (CBPs) Play an important role in the neuronal protection, differentiation and reorganization of the central nervous system, Objectives and Methods: The effects of neonatal retinal deafferentation on a CBPs, calbindm D-28k (CB), Parvalbumin (PB) and calretimn (CR), and the existence of colocalization between the CBPs were examined immunohistochemically in the rat superior colliculus. Results: On the experimental (contralateral to enucleation) side of superior colliculus, the number of CB-immunoreactive (IR) cells was reduced (77.4% compared to control), but not fibers. The number of PB-IR neurons and fibers was also reduced on the experimental side (88.5% compared to control), In the other hand, the CR-IR cells were dramatically increased (642% compared to control), but CR-IR fibers were markedly decreased on the experimental side. The colocalization between CB-CR and PV-CR was rarely observed in the superior colliculus Conclusion: These results suggest that the changes of retinotectal projection may alter the expressional pattern of CBPs in different manners; relatively stable in CB- and PV-IR neurons and plastic in CR-IR neurons.
Zhen-Yu Zheng,Keon Bong Oh,Deog-Bon Koo,Yong-Mahn Han,Kyung-Kwang Lee 한국동물생명공학회(구 한국동물번식학회) 2008 Reproductive & developmental biology Vol.32 No.1
Most studies on transgenic bioreactors have focused on expression levels of interest genes. In this study we examined whether transgenic bioreactors would inherit expression level of the Oansgene to long-term generations independently of transgene sources. We employed three transgenic mice, which were separately reported, carrying different transgenes and copy numbers, 27 kb of hLF and 22 kb of hIL-10 genomic sequences, and 1.3 kb of hTPO cDNA, respectively. Three females of the transgenic lineages crossbred with a wild-type male up to 20 generations to test transgenic frequencies of their progenies and to determine expression levels of the transgenes. Ultimately, transmission rates of kLF, hIL-10, and hTPO were 64.3+-7.0, 59.3+-9.8, and 56.1+-9.7, respectively, appeared following Mendelian pattern of inheritance. Notably, we found that levels of expressions of hLF, hIL-10, and hTPO in milk were sustained to high numbers of generations. No transgene silencing of expression was observed in every generations of all transgenic mice. In conclusion, we suggest that once established animal bioreactors could consistently transmit the transgene to continual generations, without loss of expressional activity, independently of transgene sources.
Additive effect of rod-like magnetite/sepiolite composite particles on magnetorheology
Yu Zhen Dong,Wen Jiao Han,최형진 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.93 No.-
Rod-like magnetite/sepiolite nanocomposite particles were fabricated using a chemical co-precipitationprocess and introduced as an added ingredient in a carbonyl iron (CI)-based magnetorhologicalfluid(MRF). The morphologies of the composite particles and CI-magnetite/sepiolite mixture were observedby scanning electron microscopy. Two types of MRFs with and without magnetite/sepiolite additive werefabricated, and their MR behaviors and sedimentation stability were investigated using a rotationrheometer and an optical analyzer system of Turbiscan, respectively. The results showed that the additionof rod-like magnetite/sepiolite additives enhanced both the dispersion and MR properties of CI-basedMRF, making its potential application more promising.
Zhen Yu,Xuemei Zhou,Yueqiang Wang,Guiqin Yang,Shungui Zhou 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.6
Dye wastewater normally is discharged at high temperature, but thermophilic bacteria capable of decolorizing azo dyes have rarely been isolated. Here we report a newly isolated moderately thermophilic bacterium, Novibacillus thermophilus SG-1, which had a remarkable ability to decolorize the azo dye Orange I by utilizing a large variety of organic substrates as electron donors. When Orange I served as the sole electron acceptor, almost complete decolorization occurred at 50ºC and pH 8.0 with acetate as the electron donor after anaerobic incubation of strain SG-1 for 24 h. The decolorization process followed the pseudofirst- order kinetics. The complete reduction of 0.3 mM Orange I was accompanied by a stoichiometric consumption of 0.17 mM acetate over time. The measured molar ratio (1.76) of Orange I reduced to acetate oxidized was close to the theory value of 2.0, suggesting that most of the electrons released by acetate had been transported to Orange I. Simultaneously energy generated from the electron transfer process was used to support cell anaerobic growth, which meant that azoreduction by strain SG-1 is an azorespiration process. To our knowledge, this is the first report of a thermophilic bacterium capable of azorespiration, which increases the limited number of bacteria for treating hightemperature azo dye wastewater.
Morphological Variation of Cultivated and Weedy Types in Perilla Crop Collected from South Korea
Zhen Yu Fu,사규진,이수경,이주경 한국약용작물학회 2023 한국약용작물학회지 Vol.31 No.1
Background: Perilla (Perilla frutescens L.) has been widely cultivated and used for medicinal use, aromatic, functional food, and ornamental plant in South Korea. To understand the morphological variation in the cultivated and weedy types of Perilla (CWTP) collected from South Korea, 52 accessions collected from different areas were evlauated using one quantitative and 10 qualitative characteristics. Methods and Results: Principal component analysis (PCA) was performed using the NTSYS-pc V2.1 program to detect the differences among the accessions of the CWTP. Multivariate analysis was performed using Microsoft Excel Statistical Analysis System. The cultivated P. frutescens var. frutescens and two weedy types of Perilla crop were accurately distinguished based on he 11 morphological characteristics, paricularly seed related traits, used in the study. PCA results showed that, most morphological characteristics such as weight per 100 seeds (QN1), color of leaf adaxial side (QL1), color of leaf abaxial side (QL2), stem color (QL4), flower color (QL5), seed size (QL9), and seed hardness (QL10) provided a significant contribution in the positive or negative direction on the first axis. These characteristics are considered useful for distinguishing between accessions of the CWTP collected from South Korea. Conclusions: The accessions of weedy P. frutescens var. frutescens are located between the accessions of cultivated P. frutescens var. frutescens and the those of weedy P. frutescens var. crispa on the first axis. Although the cultivated and weedy types of P. frutescens var. frutescens and the two weedy types of P. frutescens var. frutescens and P. frutescens var. crispa cannot be completely distinguished, the results provide useful information for future studies on the identification and classification of CWTP germplasm accessions collected from different areas in South Korea