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최용락,조영수,차재영 東亞大學校附設遺傳工學硏究所 1994 遺傳工學硏究 Vol.- No.1
무포자 효모인 Candida속의 효모는 aikane계 화합물을 이용하며 cellubiose나 xylose를 동화하는 등 산업적으로 이용가능성이 높다. Candida tropicalis는 세포질 인자인 plasmid가 발견되지 않으므로 염색체 유래의 자율복제기점을 non-replicative plasmid인 YIp5에 삽입시켜 S. cevisiae YNN 27에서 분리하였다. 분리된 자율 복제 기점을 Hind III과 EcoR I제한 효소로 절단하고 각각 2.7kb와 2.3kb fragment를 회수한 후 동일 제한 효소로 절단한 YIp5와 YIp32에 ligation시켜 pIKS523, pIKS527및 pIKC27을 구축하였다. 특히, pIKC27은 2.7kb의 자율복제 기점을 가진 plasmid로서 S. cerevisiae DBY746과 C.maltosa J288에서 높은 형질 전환체를 나타내었다. 기존의 plasmid와 안정성을 비교하였으며, 여러 제한효소에 의해서 얻어진 결과로서 상세한 restrition map을 작성하였다. Candida species are special interest because of its ability to metabolize hydrocarbon, covet this material into single cell protein and assimilate cellobiose and xylose as substrate. Cadida tropicalis has no native plasmid similar to 2㎛ plasmid. Therefore, Candida tropicalis is regarded as a new yeast host. An 8-kb fragment was isolated from Sal I digest of Candida tropicalis IFO 0518 genomic DNA which conferred the property of autonomous replication in Saccharomyces cerevisiae YNN 27. The vectors for gene manipulation of Yeast, pIKS 523, pIKS 527 and pIKC 27, were constructed by combinding the ARS fragment and integration plasmid YIP 5 and YIP 32, respectively. One of the recombinant plasmids, pIKC 27 (9.4kb), was capable of autonomous replication in both S. cerevisiae DBY 746 to Leu+ at a frequency of 600 transformants per ㎍ DNA, and transformed Candida maltosa J288 to Leu+ at a frequency of 230 transformants per ㎍ DNA. These results indicate that the 2.7 kb ARS element was necessary for high frequency transformation and autonomous plasmid replication in both S. Cerevisiae and C. maltosa.
조영수,최영락,손희정,김은호 東亞大學校生命資源科學大學附設 農業資源硏究所 1997 農業生命資援硏究 Vol.6 No.1
In the present, batch test was conducted to evaluate the neutralization and adsorption of heavy metals from wastewater using waste egg shells. Neutralization and removal rate of heavy metals were excellent in the increase of waste concrete amounts and a small size. It seemed that adsorption efficiencies of heavy metals were influenced by solubility. As a result on the experiments of Freundlich isotherm, the adsorption capacities(k) were Cr 3.11, Cu 2.61, Mn 3.02 and Pb 0.95, respectively and the adsorption intensities(1/n) were Cr 0.35, Cu 0.44, Mn 0.4 and Pb 0.41, respectively. In view of these results, it showed that wastes containing the similar compositions as waste egg shells could utilize the neutralization and adsorption of heavy metals in wastewater.
김중락,조영호 위덕대학교 산업기술연구소 2005 산업기술연구소 논문집 Vol.9 No.1
포항시의 철강공단에 인접한 하천인 냉천을 대상으로 식물상과 식생을 비롯하여 저서성 대형무척추동물의 군집구조와 하천의 일반 현황을 조사하였다. 이 조사 결과를 바탕으로 냉천의 특성에 적절한 친환경적 정비 방안을 제시하였다. Flora, vegetation, community structure of benthic invertebrates as well as general condition of Naengcheon stream near by the Pohang industrial complex have been investigated, On the basis of this investigation, a scheme for environment-friendly management is suggested.
이형식,조영복,김종락 慶山大學校 基礎科學硏究所 1998 基礎科學 Vol.2 No.1
민주지산 일대의 곤충상 파악을 위하여 1997년 7월부터 1997년 10월까지 조사를 실시하였다. 그 결과 총 18목 151과 540종이 조사되었다. 딱정벌레목의 곤충들이 176종으로 가장 풍부하게 나타났으며, 다음으로 나비목, 노린재목, 벌목 등의 순으로 조사되었다. 또한 보호가 필요한 종, 감소종, 및 환경부 보존 지정 곤충을 확인하였다. Insect fauna of Mt. Minjujisan was investigated from July to October in 1997. As the result, a total of 540 species of 151 families under 18 orders were identified in thr present of study. The number of species were the most abundantly revealed in the order Coleoptera, 176 species and the Order Lepidoptera, Order Hemiptera, Order Hymenoptera were followed in order.
이은숙,이영철,정지영,윤종훈,김창수,손병기,이흥락 경북대학교 센서기술연구소 1995 센서技術學術大會論文集 Vol.6 No.1
A FET type micro CO_(2) sensor based on a Severinghaus type arrangement has been prepared and its response characteristics were examined. The sensor was fabricated by formation of hydrogel layer containing bicarbonate/chloride ions on the PH-ISFET and the Ag/AgCl reference electrode prepared by the thermal evaporation and chemical chloridation methods. And then gas-permeable membrane was dip-coated on the hydrogel layer of the sensor. Gas-permeable membranes were fabricated with silicon rubber and polyurethane dissolved in toluene and THF, respectively. The CO_(2) sensor with the silicon rubber membrane showed better sensitivity and lower detection limit than the CO_(2) sensor with the polyurethane membrane for dissolved CO_(2). The sensor with silicon rubber membrane at optimal conditions showed a good response: linear range of 1.60X10^(-4) ~ 4.40X10^(-2) M CO_(2), sensitivity of 57.30 mV/decade, and detection limit of 7.50x10^(-4) M.
이상윤,라병욱,박동수,황인헌,이덕동,신영남,박성배,이동욱,박용석,박형근,손상호,권태근,채경락,정경득 慶北大學校 自然科學大學 1986 自然科學論文集 Vol.4 No.-
An Ac-type Plasma Display Panel (PDP) operating with Ne-Ar(0.1%) Penning mixture gas is fabicated. The characterics of the panel with electrodes covered with thin and thick dielectric layers are studied. The brightness of the Neon-orange light emitted by the panel measured as function of applied voltage and frequency. As an application, a graphic display system equipped with PDP showing still and moving pictures is made.