흰쥐 시상에서 Epidermal growth factor receptor면역반응 신경세포의 생후 발달에 관한 연구

박영란,정윤영,김종중,문정석,오재욱,정영욱,김주수 조선대학교 2003 The Medical Journal of Chosun University Vol.28 No.1

Background and Objectives : Epidermal growth factor receptor (EGFR), a 170-kDa transmembrane glycoprotein, appears to mediate epidermal growth factor (EGF) activity. Transforming growth factor-α and EGF produce their biological effects in numerous systems by stimulating the EGFR In this study, we examine the postnatal development of EGFR immunoreactivity in the different regions of the thalamus of the rat Materials and Methods : The present study is based on 28 postnatal cases of rat thalamus ranging from the day of birth, postnatal day 0 (P0) to 30 days (P3, P5, P10, P15, P20, P30), and these cases were compared with adult rat thalamus. Cryostat sections were processed free-floating with monoclonal antibody by immunohistochemistry Results : EGFR immunoreactivity in the thalamus of the rat showed very different patterns according to postnatal ages and thalamic areas. EGFR-immunoreactive cells appeared in the first two postnatal weeks, except the ventral posterior thalamic nuclei. In the early postnatal days, EGFR-immunoreactive cells appeared thalamic midline structures, increased progressively in the first two postnatal weeks, and followed mediolateral gradient. The mature patterns of EGFR-immunoreactive cells were achieved at P20 Conclusion : These data indicate that the maturation of EGFR-immunoreactive cells requires a relatively prolonged period of time to achieve an adult configuration. Many growth factors probably play protective or neurotrophic roles at EGFR-immunoreactive neurons of thalamus both young and adult rats In addition to difference in time of appearance in thalamic nuclei and developing pattern with mediolateral gradient suggest that EGFR-immunoreactivities are correlated with the appearance of the related functional.

척수를 손상시킨 후 꼬리정맥에 주입한 사람탯줄혈액세포가 뇌줄기에 미치는 영향

김종중,정윤영,박영란,문영민,현영식,정영욱,문정석 朝鮮大學校 附設 醫學硏究所 2007 The Medical Journal of Chosun University Vol.32 No.1

Background and Objectives: Stem cells are a valuable resource for treatment of many disease, but limited access to stem cells in some organs such as brain restricts their utility. Many approaches have been attempted to restore the function following brain stem injury (BSI) and spinal cord injury (SCI). The use of the human umbilical cord blood cells (hUCB) - a rich source of nonembryonic or adult stem cells - has recently been reported to ameliorate the behavioral consequences of stroke. Mateiials and Methods: Forty rats were divided into 8 groups: (1) SCI l+hUCB (infused 1 day post injury); (2) SCI 2+hUCB (infused 2 days post injury); (3) SCI 3+hUCB (infused 3 days post injury); (4) SCI 4+hUCB (infused 4 days post injury); (5) SCI 5+hUCB (mfusedt 5 days post injury); (6) SCI 6+hUCB (infused 6 days post injury); (7) LO+hUCB (laminectomy+hUCB); and (8) LO (laminectomy only). SCI was produced by compressing the spinal cord for one minute with an aneurysm clip calibrated to a closing pressure of 50 g. We report here that immunhistotochemical identification of fluorescent hUCB positive cells in the brain stem after compressed spinal cord injury using mouse anti-human mitochondria monoclonal antibody (MAB1273). Results: All SCI+hUCB (1-8) groups contained fluorescent hUCB positive cells in the all area of the brain stem. But especially a large number of fluorescent hUCB positive cells were observed in the whole area of the brain stem of the experimental 5 (SCI 5+hUCB) and 6 (SCI 6+hUCB)groups. No hUCB positive cells were found in the brain stem of group with non-injured spinal cord of these animals and group with laminectomy only. Conclusion: These results suggest that hUCB are potentially useful as a vector for treating a variety of the central nervous system disorders, and we are sure that continuous of stem cell study will give an best opportunity to treat the uncurable disorders in the future.

모체 Thyroxine 투여가 태아알코올효과를 가진 흰쥐 대뇌겉질 및 해마에서 NPY함유 신경세포의 생후 발달에 미치는 영향

김복,박상기,박영란,김종중,문정석,김주수,문영민,현영식,천관영,정윤영 朝鮮大學校 附設 醫學硏究所 2005 The Medical Journal of Chosun University Vol.30 No.2

Background and Objectives: Maternal alcohol abuse is considered to be one of the most prominent cause of neurobiological malformations in the postnatal and adult life of the offspring. In this study, we investigated the effects of maternal alcohol drinking on the postnatal development of NPY-containing neuron, and, the influence of thyroxine treatment on the cerebral cortex and hippocampus of pups of alcohol abused mother. Materials and Method: Time-pregnant rats were divided into three groups. Alcohol-fed group A received 35 calories of liquid alcohol diet daily from gestation day 6; control pair-fed group B was fed a liquid diet in dextrin replaced alcohol isocalorically: alcohol + T4 group C received 35 calories liquid alcohol diet and exogenous thyroxine subcutaneously. Results: Group C showed prominent NPY immunoreactivity in the cerebral cortex compared to group A and B at P7. In group C, NPY-containing neurons were widely distributed in the all layers of cerebral cortex after P14. Besides, numerical decrease of NPY-containing neuron as age increases was not found in group C. However, the decrease of NPY-containing neuron was clearly observed in group A compared to group C after P14. In hippocampus, group Band C were appeared similar patterns after P7. Additionally, in group C, NPY immunoreactivity was prominently appeared in CA2 and CA3 at P14 as compared to group B. Conclusions: The present results showed the increase of intensity and number of NPY-containing neurons in cerebral cortex and hippocampus of pups of exogenous T₄ supplemented alcohol-exposed dams as compared to control pair-fed and alcohol-exposed pups at P7. It presumably suggest that NPY-containing neurons might be regulated by the early postnatal growth stimulatory effect of the exogenously supplemented T₄. Therefore, the increase of NPY synthesis caused by maternal administration of exogenous thyroxine may ameliorate fetal alcohol effect, one of the ill effects as a result of the dysthyroid state following maternal alcohol abuse.

가정과 의생활 단원에 적용된 웹 수업자료 개발 및 실행방안

권영란,조필교 한국 가정과 교육 학회 2000 한국가정과교육학회지 Vol.12 No.3

This study was intended to develop an efficient web-based instruction materials in clothing unit. The clothing unit was chosen because this unit was supposed to be appropriate to analyze and utilize web. By using languages of html. asp, the study developed web materials to use this unit and constructed this unit into web site. Finally the study tried to examine educational effect by performing the constructed web site and representing some ways which can be applied in the classroom. The result of this study can be summed up as follows: ①The web materials were developed with steps of planning → designing → development → evaluation. In each step, detailed activities, content analysis, examples of developed materials, which presents the concrete procedures and directions were specified . ②The web materials were developed to be able to change and add up the materials in the web site. ③The web materials were developed by teaching-learning model and the lesson plan, and then they are applied. ④Instruction materials were developed according to the lesson Plan and in concrete ways.

사람의 비후성 반흔에서 면역조직화학기법에 의한 형질전환인자 알파와 표피성장인자 발현의 차이에 관한 면역조직화학적 연구

박영란,김현곤,김종중,문정석,송준섭,설경수,정윤영 朝鮮大學校 附設 醫學硏究所 2008 The Medical Journal of Chosun University Vol.33 No.2

Background and Objectives: Transforming growth factor-α (TGF-α) and epidermal growth factor (EGF) are polypeptides which interact with the epidermal growth factor receptor (EGFR) to produce their biological effects. The aim of the present investigation was to elucidate the immunolocalization of TGF-α and EGF in normal human skin, hypertrophic scar with skin graft on soft tissue defect, and postburn hypertrophic scar without graft. Methods: The data presented in this paper focused attention on differences of expression between two kinds of hypertrophic scars in relation to skin graft using immunohistochemistry. Formalin-fixed and paraffin-embedded tissues from 3 normal skin tissues, 3 hypertrophic scars after skin graft and 8 postburn hypertrophic scars without graft were immunolabelled with antibodies directed against TGF-α and EGF. Results: In normal epidermis of skin, strong TGF-α immunoreactivity (IR) was observed in all epidermal layers except the stratum (S.) basale, whereas EGF was immunopositive in a few cells over all epidermal layers. The staining for TGF-α was found in cell membrane, and EGF was seen either diffuse cytoplasmic or peripheral part of cells in normal epidermis. In hypertrophic scar after six months postoperation, TGF-α IR was observed in the upper part of S. spinosum and S. corneum, whereas EGF was diffusely expressed in the S. spinosum, also its intensity was slightly increased compared to normal skin. In hypertrophic scar without skin graft, very strong expression of EGF was detected in the S. spinosum and S. corneum, and intensity of EGF was increased when compared to normal skin and postoperative hypertrophic scar. EGF and TGF-α coexisted in the cells of S. spinosum in normal skin as well as hypertrophic scars. Double-labeled cells were increased in postburn hypertrophic scar compared to normal skin and postoperative hypertrophic scar. In addition, intensity of EGF and TGF-α double IR was the strongest in postburn hypertrophic scar. Conclusion: These results indicate that EGF may have a more complex regulatory role not only in the early stages of wound healing but also in hypertrophic scar.

18세기 정서론과 마테존

김영란 민족음악학회 1996 음악과 민족 Vol.12 No.-

젠더화된 사랑-낭만적 사랑,모성애-과 보살핌노동 : 여성복지정책적 함의 Its Implications for Women's Welfare Policy

김영란 한국사회복지정책학회 2004 사회복지정책 Vol.18 No.-

본 연구는 낭만적 사랑과 모성애가 사회적 변화에 따른 문화적으로 특수한 형태를 지닌 사랑, 즉 젠더화된 사랑(gendered love)임을 보여주고 나아가 낭만적 사랑과 모성애가 여성의 노동과 맺는 관계를 추적해 봄으로써 공/사영역에서 여성노동의 위상을 제시하였다. 나아가 여성의 보살핌노동과 관련하여 여성복지의 발전을 위한 정책적 함의를 모색하였다. 성, 사랑과 관련된 인간의 욕구는 역사적으로 다양한 방식의 체계들로 등장하였다고 한다면, 18세기 이후 사회적 상황은 그 이전시대와는 달리 이 낭만적 사랑과 모성을 기반으로 남녀간의 사랑을 규정하고 표준화하였다고 할 수 있다. 현대 사회는 핵가족만이 아닌 다양한 가족형태를 보이고 있으며, 노동시장의 유연화(flexibility)에 의한 노동불안정 증가 등으로 남성=가장(breadwinner)/여성=보살피는 자(caregiver)라는 이분법적 구도는 해체되고 있으며, 성의식의 변화 등으로 인해 낭만적 사랑과 모성애를 기반으로 한 남녀고나계는 새로운 방식으로 제기될 필요가 있다. 본 연구에서는 사회적 변화를 기반으로 젠더의 장벽을 넘어 새로운 남녀간의 사랑, 보살핌노동을 고찰해 보았다. 왜냐하면 우리사회에서 낭만적 사랑이 신비화되고 모성이 하나의 신화로 이용될 때 여성의 노동은 비가시화되거나 폄하된다고 할 수 있기 때문이다. 여성노동이 젠더화된 사랑을 기반으로 할 때 여성은 여전히 사회에서 불평등한 구조 속에 있을 수밖에 없으며 사회복지정책에서 여성복지는 여전히 불리한 위치에 놓여있게 될 것이다. The study tries to show that romantic love and maternal love is the love that has specific form culturally according to social change, that is, gendered love. It, moreover, attempts to present the position of women's labor in public and private areas by pursuing the relationship between romantic love, maternal love and women's labor. There appeared sex and love-related human desire as various forms and systems in our history, so that the love between man and woman was prescribed and standardized by the social circumstances after 18C in the different way, that is, on the basis of romantic love and maternal love. There are in the present society not only nuclear family but also various family styles, and the dichotomy (man-breadwinner, woman-caregiver) is broken up by instability of labor due to flexibility of labor-market. The relationship between man and woman based on romantic love and maternal love should be changed into a new method because of change of sex-consciousness. The study examines new man-woman's love and caring labor on the basis of social change beyond the wall of gender. When maternal love is mysticized and used as a myth and also women's labor is based on gendered love, women's labor is not exposed and estimated very low. That is the reason why women cannot be still in the unequal social structure.

신빈곤(new poverty)의 발생구조와 빈곤정책 변화에 관한 연구 : 근로빈민(working poor)을 중심으로 Focusing on the Working Poor

김영란 한국사회복지정책학회 2004 사회복지정책 Vol.20 No.-

본 연구는 사회경제적 구조변화와 빈곤형태의 변화를 고찰하고 이에 따른 우리나라의 빈곤정책의 변화방향을 모색하고자 아는데 목적을 두고 있다. 서구 유럽의 경우 1970년대 후반 1980년대에 이르러 산자유주의 시장경제 아래 사회경제적 변화가 일어났으며 이에 따라 노동시장은 재구조화되었다. 이러한 경제적 변화 가운데 새로운 빈곤층이 부상되었는데 이들은 관련되어 일하는데도 불구하고 빈곤한계선 아래에 살고 있는 근로빈민(working poor)이라고 하겠다. 이들을 신빈곤이라고 불리는 이유는 실업, 장애, 노령 등 전통적인 빈곤요인과는 달리 고용과 빈곤 양자를 함께 하고 있다는 점이다. 과거에는 빈곤탈피는 노동을 통해 가능하다고 여겨졌는데 오늘날 새로운 빈곤은 불안정한 고용형태, 저임금, 저기술을 기반으로 하고 있으며 복지정책에서도 배제되고 있어 기존의 빈곤과는 다른 형태를 취하고 있다. 이러한 신빈곤의 원인은 세계화에 따른 노동시장의 유연화, 산업구조의 변화에 따른 제조업의 약화와 불안정한 서비스업의 증가, 기존 복지시스템의 한계 등에 의한 것이다. 우리나라의 경우도 1990년대 들어오면서 서구와 마찬가지로 변화되기 시작하였는데 1997년 IMF 경제위기 이후 노동시장의 유연화는 노동자들의 비정규화를 가져왔으며 2002년 현재 비정규직은 56.6%로 정규직을 능가하고 있다. 또한 이들은 불안정한 고용, 저임금, 열악한 노동환경 속에서 빈곤의 위엄성에 노출되어 있으며, 영세자영업자 역시 빈곤층으로 전락하고 있다. 2001년 전체 빈곤가구 중 55.7%가 근로빈곤 가구로 나타났는데 키들의 빈곤은 더욱 더 가속화되고 있으며 그 수요도 점차 증가하고 있다. 현재 우리나라 복지정책은 수혜대상에 있어 안정적인 정규직중심으로 되어 있어 비정규직과 영세자영업자는 복지수혜대상에서 배제되고 있다. 따라서 본 연구는 세계적인 현상으로 대두되는 신빈곤에 대안 정의, 발생원인 등을 고찰하고 한국사회에서 신빈곤의 핵심층인 근로빈민을 대상으로 이들의 실태연구 및 현 재 복지정책의 한계를 제시하고자 한다. 나아가 현재의 근로빈곤층의 탈빈곤을 위해서는 새로운 차원의 복지정책이 필요하다는 전제 아래 우리나라의 새로운 복지정책의 방향을 제시하고자 한다. The object of the study is to examine changes of social-economic structure and poverty-form in the West, and then to explore ways towards desirable poverty policy in Korea. In the latter half of 1970s to 1980s, Europe experienced social-economic change under new liberal market-economy, so it was necessary that its labor market was restructurized and new poor class appeared. They were, for the most part, the working poor who lived under the boundary line of poverty notwithstanding that they worked in employment. They were in poverty as well as in the employment, that is, their poverty elements is very different from conventional poverty elements such as the unemployment, the disable and the old-aged. That is the reason why they were called new poor class. It seemed in former times that they were able to overcome their poverty if they worked hard. The new poverty, however, is not only based on under-employment, low-payment and low-skill, but also excluded from welfare policy. The new poverty originates in flexibility of labor market due to globalization, weakening of the manufacturing industry, increasing of unstable service industry, and limits of welfare system owing to change of industrial structure. In 1990s Korea began to change in the same way as the West, especially after the economic crisis of IMF in 1997 laborers were irregularized by flexibility of labor market, and in 2002 irregular employment surpassed regular employment as 56.6% in the rate. and after all they are in the danger of poverty because of unstable employment, low-payment and deteriorated labor-environment. Besides a small-scale self-businessman sinks to the poor class. In 2001 the rate of working poor households is 55.7% of total poor households, and their poverty becomes serious and increases gradually. In present days Korean welfare policy regards regular employment as an object of welfare benefit and excludes the small-scale self-businessman. Therefore the study relates definition and reason of new poverty, which appears as global phenomenon, and shows actual conditions of the working poor who hold the core of new poor class and the limits of present welfare policy. It, moreover, suggests new welfare policy for working poor class to escape from their poverty.

흰쥐 편도체에서 칼슘결합단백인 calbindin-D28K함유 신경세포의 생후 발달에 관한 면역조직학적 연구

정윤영,김종중,문정석,박영란,정상봉,김현곤,박상수 조선대학교 2001 The Medical Journal of Chosun University Vol.26 No.1

Background and Objectives : The calcium-binding protein, calbindin-D28K(CB) is widely distribution of CB in rat amygdala during postnatal maturation, in order to provide an insight into the developmental role it may play in the amygdaloid neurons. Materials and Methods : The present study is based on 28 postnatal cases ranging from the day of birth, postnatal day 0 (P0) to 30 days (P5, P10, P15, P20, P30), and these cases were compared with adult rat amygdala. Cryostat sections processed free-floating with monoclonal antibody by immunohistochemistry. Results : CB-immunoreactive neurons were already found at birth in all amygdaloid nuclei except the intercalated nucleus. The intensity and number of immunoreactivity of CB-containing neurons increased during the first 10 days of postnatal life but dramatically decreased at P15. Mature patterns of CB-immunoreactive neurons were achieved at P20. In the adult amygdala, CB-immunoreactive neurons were distributed in almost all amygdaloid nuclei except the intercalated nucleus. Conclusion : The present results show early appearance of CB immunoreactivity in the amygdala. It may be related to the early appearance of CB immunoreactivity in many other brain regions, and suggests that CB may play a functional role during the development of the nervous system including amygdala.

남북한 민속놀이 비교 연구

최영란 한국체육사학회 2001 체육사학회지 Vol.7 No.-

The traditional folk recreation reflect its own race's racial emotions and ideas, and the Korean folk recreations also express his tradition and have been banded down as one part of the history. The purpose of this thesis is to research the present conditions of the Korean folk recreation that is famous for its a long history. Particularly, the main focus of this thesis's dissertation is on clarifying the real conditions in inheritance of the Korean folk recreation and its modernized process, by comparing the examples of the North Korean recreation with those of the South Korean recreation. <The Folk Recreation> in {The General Survey on the Korean Folk} that was published in the South Korea classifies the whole inherited folk recreation into four categories; the recreation for children the recreation for the adults, sports games, and acrobatics. One of the main characteristics that are shown in the South Korean folk recreation tradition is that the trait of 'pastime', or 'amusement' is stronger than that of the North Korea. This reinforcement of this entertaining and amusing trait is closely related with the sudden and drastic spread of mass culture and its great effects. Ultimately, all of these result are grounded upon the social and economic situations that have rapidly and continually changed into a highly developed industrial aspect, since the modernization. However, the folk recreation in several provincial districts are clearly distinguished from that in urban areas, which means some rural areas have a function of devotion, yet. The folk recreation's characteristics in the South Korea, above all, include 'amusement' and 'entertainment', and another main characteristic is the 'ritual' characteristic. Many audience are still voluntarily participating in these folk recreations for the purpose of fulfilling their ritual consciousness. Therefore, even though this ritual side's characteristic is weakened, compared with the past old ages, some folk recreation events of the South Korea are still functioning as a ritual or religious effect. One more characteristic that is companied together with 'entertainment', 'amusement', and 'ritualism' is 'the artistic'. The folk recreation in the South Korea has been inherited as a form of 'folk art', and being recreated into a form of 'public performance'. On the contrary, the common characteristic of the North Korea is 'an enormous scale'. The North intend to unite social groups through the folk recreation activities, cultivate friendship among social members as well as noble dispositions of the people. And these distinguishedly social function and purposeful consciousness have made the North Korean's folk recreation have a greatly reformed characteristics, compared with the past. The most noticeable characteristic is 'the physical educational-ization of the folk recreation' that embodied the idea of huge scale. Following Kin II Seong's instruction, "The racial folk physical culture, such as 'the traditional seesaw games', 'swing', and 'tug of war' should be encouraged for people", the North Korean developed the traditional folk recreation into one item of physical education for students. The next characteristic is 'art-ification' of the folk recreation Kim Jeong II has instructed people to art-ify the folk recreation. The representative work is {The Song of Seasons}, a suite for folk dance, created by Peeboda Opera Company, in which work the game of yut, the swing game, the traditional wrestling game are transformed into artistic work. The last characteristic is 'acrobatic-fication' of the folk recreation. The acrobatics of the North Korea include horse-riding, hard-craft, foot-craft, and sword-craft, which adopt folk recreation forms in an indirect way. The most important function of the North Korean folk recreation is the social revolutionary one. Therefore, the North Korean folk recreation is recreated under this kind of invincible idea. While the South Korean folk recreational activities have the characteristics of 'amusement', 'entertainment', and 'rituality', those in the North Korea have been recreated in a huge scale, for the purpose of establishing a nation on the ground of socialism. Not only this line of difference but also some common points can be pointed out. The examples of the common point are the Korean wrestling(SSiLeum), which folk recreation is performed in the form of game, and other various dances that ate recreated in a modernized sense.