The C- and G-value paradox with polyploidy, repeatomes, introns, phenomes and cell economy

Ik‑Young Choi,Eun‑Chae Kwon,Nam‑Soo Kim 한국유전학회 2020 Genes & Genomics Vol.42 No.7

Background The apparent disconnection between biological complexity and both genome size (C-value) and gene number (G-value) is one of the long-standing biological puzzles. Gene-dense genomic sequences in prokaryotes or simple eukaryotes are highly constrained during selection, whereas gene-sparse genomic sequences in higher eukaryotes have low selection constraints. This review discusses the correlations of the C-value and G-value with genome architecture, polyploidy, repeatomes, introns, cell economy and phenomes. Discussion Eukaryotic chromosomes carry an assortment of various repeated DNA sequences (repeatomes). Expansion of copies of repeatomes together with polyploidization or whole-genome duplication (WGD) are major players in genome size (C-value) bloating, but genomes are equipped with counterbalancing systems such as diploidization, illegitimate recombination, and nonhomologous end joining (NHEJ) after double-strand breaks (DSBs). The lack of these efficient purging systems allowed the accumulation of repeat DNA, which resulted in extremely large genomes in several species. However, the correlation between chromosome number and genome size is not clear due to inconsistent results with different sets of species. Positive correlations between genome size and intron size and density were reported in early studies, but these proposals were refuted by the results with increased numbers of species, in which genome-wide features of introns (size, density, gene contents, repeats) were weakly associated with genome size. The assumption of the correlations between C-value and gene number (G-value) and organismal complexity is acceptable in general, but this assumption is often violated in specific lineages or species, suggesting C- and G-value paradoxes. The C-value paradox is partly explained by noncoding repeatomes. The G-value paradox can also be explained by several genomic features: (1) one gene can produce many mature mRNAs by alternative splicing, and eukaryotic gene expression is highly regulated at both the transcriptional and translational levels; (2) many proteins exert multiple functions during development; (3) gene expansion/contraction are frequent events in the gene family among evolutionarily close species; and (4) sets of homeotic genes regulate development such that organismal complexity is sometimes not clear among organisms. A large genome must be burdensome in terms of cell economy, such that a large genome constraint results in the distribution of genome sizes skewed to small genomes. Moreover, the C-value can affect the phenome. A strong positive correlation has been recognized between genome size and cell size, but the relationship is weak or null with higher-level traits. Additional analyses of the relationship between the C-value and phenome should be carried out, because natural selection acts on the phenotype rather than the genotype. Conclusions Dramatic advancement in genomics has given some answers to the C-value and G-value paradoxes. We know the mechanisms by which the current genomes have been constructed. However, basic questions have not yet been fully resolved. Why have some species retained small genomes yet some closely related species have large genomes? Random genetic drift and mutational pressure might have affected for genome size in the limited population size during evolution; thus, genome size may be quasiadaptable rather than the best adaptive trait.

Effects of I- and W-Phases Under Identical Conditions on Microstructure and Mechanical Properties of As-Cast Mg–Zn–Y Alloys at Room and Elevated Temperatures

Young‑Gil Jung,Wonseok Yang,Jae Ik Hyun,Shae K. Kim,Hyunkyu Lim,Do Hyang Kim 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.12

Three alloys (Mg–6Zn–1.2Y, Alloy I; Mg–3.65Zn–1.65Y, Alloy II; and Mg–4.3Zn–1.4Y, Alloy III) with same volume fractionand grain size were designed to evaluate the effects of the I-phase (Mg3Zn6Y) and W-phase (Mg3Zn3Y2), which are themajor phases in Mg–Zn–Y alloys, on the mechanical properties. The tensile strength of Alloy I with the I-phase at roomtemperature was the highest among the tested alloys because the coherent interface between the I-phase and the α-Mg phasewas more resistant to cracking than the incoherent interface between the W-phase and the α-Mg phase. A cross-sectionalmicrostructure analysis of a sample that was tensile-tested at 423 K revealed that the morphology of the I-phase remainedrelatively stable. In contrast, the W-phase was broken and fragmented during the tensile test at 423 K because it had higherbrittleness under the test conditions. Therefore, the tensile and creep properties of Alloy I at 423 K were better than thoseof Alloys II and III containing the W-phase. According to the results, the I-phase in the Mg-Zn-Y alloy was more beneficialto the mechanical properties at room temperature and 423 K than the W-phase.

위암 환자에서 세포성 면역 기능에 관한 연구

이재익,윤일국,이종선,김종완,장준,안정기,송민호,정현용,이헌영,김삼용,김영건 충남대학교 암연구소 1991 癌共同硏究所 硏究誌 Vol.1 No.1

We performed a variety of lymphocyte stimulation tests, delayed hypersensitivity skin tests, and enumeration of several lymphocyte subpopulations in 21 patients with gastric cancer who did not receive any form of anticancer therapy, and in 20 healthy controls. The gastric cancer patients had significantly decreased number and total score of positive reactions in delayed hypersensitivity skin tests compared with the healthy controls. The percent of CD_(4) positive lymphocytes in the gastric cancer patients was significantly decreased compared to the normal control subjects, but it had no correlation with the total score of delayed cutaneous hypersensitivity reactions. The ratio of helper to suppressor cells was lower in the cancer group. The uptake of 3H-thymidine was markedly depressed in cancer patients when stimulated with various mitogens. There was little correlation between any of the stimulation tests and any of the lymphocyte subpopulation proportions to delayed hypersensitivity cutaneous reactions. Optimal proliferative response was found in lymphocytes stimulated with 10 micrograms of concanavalin-A and 10 microgram of phytohemagglutinin. Advanced stage patients had greatly depressed delayed hypersensitivity skin reactions and proliferative responses to mitogens compared with localized diseases. These results suggest that gastric cancer patients have depressed cellular immune functions, which mainly result from the decreased helper cells and defects in functional proliferative response to mitogens. Interleukin-2 and interferon-gamma restored the in vitro proliferative response of lymphocyte in patients with gastric cancer.

회맹장 판막에 발생한 아니사키스증 1례

이재익,박정하,김준영,조영완,진한영,강명주,정은욱,박석주,양성연 인제대학교 2006 仁濟醫學 Vol.27 No.-

Anisakiasis refers to parasitic infestation by nematode larvae that belongs to the subfamily Anisakinae, and this condition is seen in people who eat inadequately prepared or raw salt-water fish. While gastric anisakiasis is commonly diagnosed by endoscopic technique, intestinal anisakiasis is rare because it is less common and the clinical and radiologic features are poorly understood. The authors report a case of human anisakiasis involving the ileocecal valve in 62-year-old man who had the history of eating raw cuttlefish.

융모성림프구를 보이는 비장변연부 림프종 1예

진한영,손창학,주영돈,박정하,이재익,조영완,이원식 인제대학교 2006 仁濟醫學 Vol.27 No.-

Splenic marginal zone lymphoma is very rare B-cell lymphoma, characterized by an indolent clinical course. Clinical features are moderate-to severe splenomegaly, absolute lymphocytosis, and bone marrow intrasinusoidal infiltration of lymphocyte. We report the case of a 37-year-old male with SMZL. He complained of LUQ pain, who had a massive splenomegaly and moderate lymphocytosis in peripheral blood. Immunophenotyping findings and morphologic findings were consistent with SMZL. He underwent splenectomy and received chemotherapy with cyclophosphamide for eight months. Now, one year later after surgery he has a nearly normal blood count with no treatment.

간세포암의 고주파 열치료 후 천자침 경로를 통한 전이 1례

조영완,박석주,진한영,김준영,이재익,강명주,박정하,윤정희,박성재,지삼룡,이연재,이상혁,설상영 인제대학교 2006 仁濟醫學 Vol.27 No.-

Radiofrequency ablation(RFA), as a form of minimally invasive therapy for hepatocellular carcinoma, has become an important treatment modality. Because of limitation of surgery, RFA has become standard therapy for hepatocellular carcinoma in some situations. But there are some complications of RFA such as bleeding, infection, hematoma, adjacent organ thermal damage including intestinal perforation, needle track seeding, and so on. There are few reports in the literature that systematically evaluate the incidence of needle tract seeding and its associated risk factors. And only 2 cases of needle tract seeding of hepatocellular carcinoma after RFA were reported in Korea. We report a case of needle tract seeding of hepatocellular carcinoma after RFA.

어린이집 영유아의 성장ㆍ발달에 관한 종단적 연구(Ⅱ)

고영인,임재택,조희숙,황해익,정계숙 부산대학교 영유아보육연구소 1998 영유아보육연구 Vol.4 No.-

The purpose of this study was to investigate longitudinal growth and development of young children and follow-up effects of the educare program in the PNU Day Care Center. Particularly, the first objective of this second report of study was the comparison the entire domains of development with age between the Oct. of 1995 and the Dec. of 1998. The second objective was to analyze the developmental .changes of 5 children who entered the center in 4 years old since Oct. 1995. The subjects of this study were 448 children(241 boys, 207 girls). The instruments of this study were the KISC and KEDl-WISC(for intelligence test), the Learning Readiness Test, the Social and Emotional Scale, Height Scale and Weight Scale( for physical growth). The tests were carried out 2 times per year (the early of the year and the late of the year). Results from comparsion across the 1st through 7th tests during 3 years indicated that the mean of the height was increased about 1-8cm for all of 2-5 years old children. The mean of the weight also was increased about 1.5-4.0kg except 2 years old children. The intelligence score (T score) by KISC was increased around 6-42 points except 6 years old boys and girls. In the intelligence score by KEDl, there was improvements of average 8 points for 7 years old children. In the learning Readiness Test of 5, 6 years old children, the scores all of children except 6 years old girls got were decreased. The scores of 6 years old girls were incresed 6 points in the Dec. of 1998. In the Social and Emotional Scale, there was big improvement of scores between in the 1st test and in the 7th test. These results were discussed in the view of sample size, developmental characteristics, test instruments and educare program of the PNU day care center.

노인의 생활체육활동 참가와 신체적, 정신적 기능상태의 관계

이영익(Lee, Young-Ik) 한국노년학회 2008 한국노년학 Vol.28 No.4

이 연구는 노인의 생활체육활동 참가와 신체적, 정신적 기능상태의 관계를 구명하는데 그 목적이 있다. 본 연구의 조사 대상은 2008년 현재 서울지역에 거주하는 만 65세 이상 노인을 모집단으로 설정한 후, 유층집락 무선표집법(systematic stratified cluster random sampling)의 방법을 이용하여 양로원, 노인정, 노인대학 집단 등을 중심으로 표집틀(sampling framework)을 작성한 후, 생활체육참여자 180명, 비참여자 180명 총 360명의 표본을 추출하였다. 독립변인으로는 여가스포츠활동 참가여부와 참가정도(기간, 빈도, 강도)로 분류하였으며, 그리고 종속변인인 신체적 기능상태는 정경희 등(1998), 김양례(2005a)등이 사용한 문항을 토대로 본 연구에 맞게 수정, 보완하여 사용하였다. 정신적 기능상태는 인지기능 척도와 우울척도를 사용하였다. 먼저, 인지기능 척도는 개인이 지능을 이용하여 당면문제를 해결하는 정도로써, 한국판 MMSE-K(Mini Mental State Examination-Korea)를 사용하였으며, 우울증 척도는 Beck(1978)가 개발하고 이영호, 송종용(1991)이 번안한 설문지를 조규황(2003)이 한국 실정에 맞게 수정한 설문지를 본 연구의 목적에 맞게 수정ㆍ보완하여 사용하였다. 연구결과의 분석을 통해서 다음과 같은 결론을 얻었다. 첫째, 노인의 생활체육활동 참가여부에 따라 신체적ㆍ정신적 기능상태에 부분적으로 영향을 미친다. 즉, 생활체육활동에 참가하는 노인집단이 참가하지 않는 노인집단보다 일상생활수행능력ㆍ인지기능과 우울증 하위변인 중 인지적ㆍ신체적ㆍ행동적 요인이 높았다. 둘째, 노인의 생활체육 참가정도는 신체적ㆍ정신적 기능상태에 부분적으로 영향을 미쳤다. 즉, 생활체육 참가정도에 따라서 신체적, 정신적 기능상태의 하위변인인 일상생활수행능력에서는 참가기간, 참가강도가 인지기능에서는 참가기간, 그리고 우울증의 하위변인 중 인지적ㆍ정서적 요인에는 참가기간, 참가빈도가 영향을 미쳤고, 신체적ㆍ행동적 요인에는 참가기간, 참가빈도, 참가강도 모두 영향을 미치는 것으로 나타났다. The purpose of this study was to study the Relationships between Sport for All Participation and Physical and Mental Function affecting the Elderly's Self-perceived Health. For accomplishing this purpose of this study, the 308 subjects were selected by the multi-stage stratified cluster sampling method from the Seoul, in the years of 2008. presently. Out of the 360 questionnaires responded to, only 308 were used for data analysis because 52 were responded to incompletely or incorrectly. The survey questionaries were used to collect data. The questionary for physical function state were developed and standardized by kung-hee chung(1993), yang-lae Kim(2005). And mental function state were divided two parts; self-perceived scale and depress scale. The questionary for self-perceived scale were based on MMSE-K(Mini Mental State Examination-Korea). The questionary for Depression were based on Depression Scale developed by Beck(1978), adapted in Korean and standardization by Lee Young-ho & Song Jong-yong(1991), regulated by Jo Gyu-whang(2004). From the analysis of this study, the following conclusions were obtained: First, according to the elderly people's participation in leisure sport activities, it partially affected physical and mental function state. Second, according to the degree of elderly people's participation in leisure sport activities, it partially affected physical and mental function state.

Effect of Cr₂O₃ Content on Densification and Microstructural Evolution of the Al₂O₃-Polycrystalline and Its Correlation with Toughness

Seo, Mi-Young,Kim, Hee-Seung,Kim, Ik-Jin The Korean Ceramic Society 2006 한국세라믹학회지 Vol.43 No.8

The effects of $Cr_2O_3$ on the microstructural evolution and mechanical properties of $Al_2O_3$ polycrystalline were investigated. The microstructure of $Al_2O_3-Cr_2O_3$ composites (ruby) was carefully controlled in order to obtain dense and fine-grained ceramics, thereby improving their properties and reliability with respect to numerous applications related to semiconductor bonding technology. Ruby composites were produced by Ceramic Injection Molding (CIM) technology. Room temperature strength, hardness, Young's modulus and toughness were determined, as well as surface strengthening induced by thermal treatment and production of a fine-grained homogenous microstructure.

한국 최초의 근대식 의사 김익남의 가문에 관한 연구

이영아 ( Lee Young Ah ),황상익 ( Hwang Sang-ik ) 아시아문화학술원 2017 인문사회 21 Vol.8 No.4

김익남은 한국 최초의 근대식 의사이자, 근대의학 교육의 선구자이다. 그는 과거시험 폐지 이전에 `가숙(家塾)`에서 3년 동안 한의학을 공부했고, 과거제도가 폐지되자 곧 관립일어학교에 들어갔으며, 뛰어난 일본어 능력과 근대교육에의 적응력으로 당시 파일(派日) 유학생들 중에서도 가장 두각을 나타내어 한국인으로는 최초로 근대의학을 정식으로 배운 의학자가 되었다. 그런데 그동안 전형적인 양반 가문인 청풍김씨 가문의 후손으로 알려져 왔던 김익남의 직계 조상을 조사해 본 결과 그는 기술직 중인 가문의 후예로 보는 것이 타당함을 알 수 있었다. 또한 잡과방목 등에는 그의 직계조상들의 본관이 해주김씨로 기록되어 있었다. 이러한 점에서 김익남은 의과, 역과, 운과(음양과) 등으로 세전되어 온 기술직 중인 가문인 해주김씨의 후손이라는 사실을 발견하게 되었다. 한국근대의학의 선구자인 김익남의 조상들은 지금까지 알려졌던 것과는 달리 약 300년 동안 전형적인 기술직 중인 가문으로 살아왔다. 그리고 김익남이 1890년대 당시로는 쉽지 않았을 일본유학을 결정하고 낯선 유학생활을 무사히 마치고 한국인으로서는 최초로 근대식 의사가 된 데에는 중인 가문 출신이라는 배경이 긍정적으로 작용했을 것이다. Unlike what has been known so far, the ancestors of Dr. Kim Ik-Nam, the most forerunning figure in the modern Korean medical history, had lived not as yangban class but as the typical jungin class for almost 300 years. It is thought that they could incorporate into Kim lineage of Chungpoong, the famous yangban family, through the achievements of successive generations as professional middle class, and their desire of status ascension based upon the achievements. Dr. Kim Ik-Nam`s jungin class background is considered to have positively influenced on the decision of studying abroad to Japan to be the first modern Korean medical doctor, which was very difficult in the 1890s. And it would be the same case to his cousin`s son Kim Kyu-Sik, the famous independence activist.