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이근광,김영길,박성우,최민순 한국어병학회 1994 한국어병학회지 Vol.7 No.2
뱀장어의 울혈증 발병 원인구명을 위하여 서해안 지역 5 개 양만장을 대상으로 아가미 울혈증 발병현황과 수질등 역학조사를 실시하였고, 한편으로는 스트레스(handling 과 수온차) 및 병어에서 분리한 아가미 마쇄액을 건강어의 복강에 주사하여 울혈증 발병률과 CHSE-214 에 접종하여 세포변성을 조사하였다. 조사한 양만장의 아가미 울혈증 발병률은 30-80%로서 사육시의 수온과 선별시의 수온차가 클수록 발병률이 높았으며, 수질환경과 본증의 발병과는 뚜렷한 상관관계가 없었다. 또한 발병률이 높은 양만장의 뱀장어는 Ht, Hb, Tp, Alb, Mg 및 Glu 치등이 낮은 반면 GOT, GPT, Ca 및 Met-Hb 치등은 높게 나타나는 경향을 보였으나, 반드시 아가미 울혈증에 비례하여 증감되지는 않았다. 스트레스(handling 과 수온차)를 가함에 따라 아가미 울혈증이 실험적으로 유발되어졌으며, 발병률은 50-70% 이었다. 이러한 스트레스 조건하의 어류의 혈액학적인 성상은 울혈증 발병빈도에 비례하여 Ht, GOT, GPT, Met-Hb, Alb 및 Glu 치등은 증가되었으나, Tp, Hb 및 Mg 은 감소하는 경향을 보였으며, 온도차가 클수록 cortisol 량이 증가되는 경향을 보였다. 그러나, 병어의 아가미 마쇄액을 복강주사 및 CHSE-214 에 접종하였던 바 각각 울혈증의 발병 및 세포변성이 전혀 관찰되지 않았다. 이러한 결과는 선별시 handling 과 수온의 급변이 혈중 cortisol 을 상승시켜 혈액의 전해질 변화를 초래케하므로써 혈액의 흐름을 완만하게 되고 동정맥계로의 혈액유입량이 증가하게 되어 중심정맥동이 확장되므로써 울혈이 형성되는 것임을 시사한다. In order to elucidate the outbreak mechanisms of a new disease which is characterized by an intense congestion in central venus sinuses(CVS) of gill filaments in cultured eel. these experiments were carried out; epidemically surveyed on the cultured eel farms in the vicinity of Kunsan city and experimentaliy outbreaked the disease in the stressful condition such as thermal and handling shock and innoculated the supernatant from the homogenate of naturally severe congested gill into eels and onto the monolayer of the CHSE-214. Although the frequency of congestion in eels of B, C, D and E farms were higher than in eels of A farms, the water qualities (stocked and cultured water) among farms were not a great difference. In eels of B, C, D and E farms, the values of haematocrit (Ht), haemoglobin (Hb), total protein (Tp), albumin (Alb), glucose (Glu), magnesium (Mg) were lower and the values of calcium(Ca), methemoglobin(Met-Hb), glutamic pyruvic transminase (GPT), glutamic oxalacetic transminase (GOT) higher than in eels of A farms. These valules have not related to the frequency of congestion. An intensive congestion and dilataton in CVS of gill filaments in experimentally handling-stressed eels produced similar histopathological changes to those observed in the spontaneously diseased eel, but not in eels experimentally injected with filtering contents. The cytopathic effect on the CHSE-214 was not observed. In stressed eels the congestion of gill was increased in relation to either the decrease ranges of water temperature or the incerase in accllimated times. And increase in Ht, Met-Hb, Alb, Glu, GOT and GPT and decrease in Mg, Hb and Tp were found, which had a close relationship to congestion of gill. Cortisol were increased according to the decrease ranges in acclimated water temperature. From these results, decrease in water temperature during selection placed eels upon the stressed condition, made increase in ionic strength in blood stream, and CVS was dilatated owing to the increased blood inflow.
西海 沿岸의 養殖場 環境 調査 : 2. 高亭理 海域 김養殖場의 水質環境
金容浩,金榮吉,李廷烈,金鍾連 군산대학교 1990 群山水産專門大學 硏究報告 Vol.24 No.1
To understand the effects of oceanographic condition on the harvest of laver and establish the basic environmental data for cultivation farm of laver, a series of survey was carried out in Gojeong-ri sea area, west coast of Korea from January to September, 1987. Variation of water temperature in Gojeong-ri sea area was 3.8-25.8℃, pH was 7.8-8.4, and transparency was 1.3-3.1m. Salinity was changed from 19.57 to 31.99% and lower remarkably in July and August owing to a heavy rain. The concentration of DO in surface water wart varied from 9.4-10.1㎎/ℓ and in lower layer was 8.0-9.99㎎/ℓ . COD was 0-4.28㎎/ℓ by alkalic modification of potassium permanganate method. Distribution of total suspended solid was 13.7-488.7㎎/ℓ and the rate of light penetration in 1m water column was 7.2-15.5%. The concentration of nutrients were changed in 0.48-23.63㎍-at./ℓ for ammonia nitrogen, 0-4.45㎍-at./ℓ for nitrite nitrogen, 0.39-3.13㎍-at./ℓ for nitrate nitrogen, 0-21.42㎍-at./ℓ for phosphate, and 0.84-157.32㎍-at./ℓ for silicate. It was showed high concentration in summertime, raining season in general. The concentration of chlorophyll a was 0.39-20.62㎎/㎥. Total 57 species of phytoplankton and 21 groups of zooplankton were identified from collected samples. Dominant species were Coscinodiscus radiatus, Eucampia zodiacus as a phytoplankton and Noctiluca scinitillans, Cladocera, Copepods sp. as a zooplankton. The total standing crop of phytoplankton was ranged of 5,500-1,524,400cells/ℓ and that of zooplankton was 3,000-43,000 individuals/㎥.
조개類에 寄生하는 Bacciger屬 吸忠類에 關한 硏究 - Ⅱ Latermla limicola REEVE에서 檢出되는 Bacciger harengulae에 대하여
金榮吉 군산대학교 1982 群山水産專門大學 硏究報告 Vol.16 No.1
Cercaria larvae of Bacciger parasitic to the littoral spoon clam, Laternula limicola REEVE were studied in terms of their morphology and incidence of infection rate. The host bivalves were examined from Naechodo, Gunsan from September 1981 to February, 1982, The cercaria were morphologically compared to those found in the others clams, i.e. Meretrix lusoria, Tapes philippinarum, and Solen strictus. It is conformed that the cercariae were identified to be Cercaria harengulae. Head size of the cercaria was 250X150μm, and tail size was 480X57μm. The tail was armed with 27 pairs of tufts of setae, the tuft being composed of 6∼8 fin rays. Oral sucker, ventral sucker, intestine and a pair of testis were distinctive. The flam cell arrangement is represented by a formula, 2[3+3)+(3+3)]=24. Mean infection rate to L. limicola was 14.8%. However the lager bivalves showed the higher infection rate. In conclusion L. limicola is newly added to the hosts of Cercaria harengulae of Bacciger sp.
금강하구산 어패류에 기생하는 흡충류에 관한 연구 : 一報 문절망둑에서 검출되는 Echinostoma속 흡충에 관하여
金榮吉 군산대학교 1975 群山水産專門大學 硏究報告 Vol.9 No.2
In the intestine of 59 fishes of Acanthogobius flavimanus (T. et SCHLEGEL) gathered from Sun yearn-Ri, the lower cours of the Kum river over 5 times from March till July in 1975, 5 Encysted larvae of Echinostama sp were detected. The size of Encysted larvae is 650×610μ and Encysted larvae 1,750×475μ. Head collar arranged in 2 lines composed of 37.
金榮吉 군산대학교 1974 群山水産專門大學 硏究報告 Vol.8 No.2
I have collected the Molluscan shells at the Go-Gun San Islands in the Western Sea. List in recorded are as follows: 1.Total species in appearance are 73, such as Loricates 1, Gastropoda 37, Lamellibranchia 35. Gastropoda and fixed sedentary is distributed much more than submerged sedentary. 2.Brevimyurella lischkeana(PHILIPPI), Euhadra congenita hikonis (KO-BELT), Rapana bezoar(LINNEUS) are Listed in Unrecorded species in Korea.
김영길,박성우,최민철 한국어병학회 2002 한국어병학회지 Vol.15 No.3
한국산 어류에 감염된 포자충의 종류와 병해를 조사 할 목적으로 2001년 9월부터 2002년 8월까지 양식중인 정읍산 메기와 만경강 하구의 기수 구역에 서식하는 자연산 문절망둑을 조사하였다. 이들 어류에서 한국 미기록종인 포자충의 감염이 확인되었다. 즉 메기의 장상피조직에서 Myxobolus miyairii Kudo, 1919가, 문절망둑의 피하 근육조직에서 Henneguya tridentigeri Ozaki & Ishizaki, 1941가 검출되었다. 메기에 기생 된 M. miyairii는 장 상피조직에 42~77(56)㎛ × 59~93(73)㎛ 크기의 백색 영양체가 무수히 관찰되었고, 포자의 길이는 11.0~12.0(11.3)㎛이었다. 어체 기생율은 86.7~90%였으나, 감염어는 별다른 병변을 발견 할 수 없었다. 문절망둑에 기생 된 H. tridentigeri는 몸통부 근육에 백색 타원형의 영양체를 형성하고 있었으며, 크기는 1.04~4.45(2.43)×0.43~2.21(0.95)mm이었다. 포자는 장 타원형으로 포자각의 후각에 2개의 돌기를 그 크기는 10.0~12.0(10.7)×6.0~8.0(7.1)㎛, 돌기의 길이는 12.0~24.0(16.9)㎛이다. 문절망둑에서의 기생율은 76.9~90.9%이었고, 감염어는 외관상 별다른 이상이 발견되지 않았다. Thirty five catfish (Silurus asotus) cultured in a fish farm of Jungeup and 222 wild goby (Acanthoguobius flavimanus) caught from the estuary of Mankyoung River, Chonbuk province of Korea were examined the prevalence of myxosporidian infestation. A lot of while spherical myxosporidian cysts in the intestinal epithelium of catfish and the muscle of goby were easily found. The cysts size was 56 × 73 (42~77 × 59~93 ㎜) in catfish and 2.43 × 0.95 ㎜ in goby. The infection rate(%) was 88.4 in catfish and 89.4 in goby. The Myxosporidians from the intestine of catfish and from the musculature of goby were identified as Myxobolus miyairii and Henneguya tridentigeri, respectively, on the basis of the shape and structure of the spores under a light microscope. Dimension (㎜) of fresh spores of Myxobolus miyairii: length, 11.0~12.0; width, 5.0~6.0; thickness, 3.5~4.0; polar capsule: length, 5.0~5.5; thickness, 1.5~2.0; polar filament: length, 40~45. Dimensions (㎛) of fresh spore of Henneguya. tridentigeri: length, 10.0~12.0; width 6.0~8.0; thickness, 4.8; polar capsule: length, 4.6; thickness, 2.6; iodinophilic vacuole: 2.8 × 2.4. No significant pathological change was found from the infected fish. This is the first report on M. miyairii and H. tridentigeri from fish species in Korea.
春季 錦江下流域의 水質 및 Diatom의 組成變化에 關하여
金榮吉,金鍾培 군산대학교 1978 群山水産專門大學 硏究報告 Vol.12 No.2
The monthly observation on the composition quantity of Diatoms and chemical contents was done at 7 stations in the below stream area of Gum River from April to June, 1978. The total numbers of Diatom appeared by observation were 27 species ; neritic planktons(27), and oceanic planktons(2) and the composition organism of total diatoms was 1,600Cells/ℓ∼41,700Cells/ℓ. The surface water temperature is 6.8∼20.8℃ at St. 3, 13.4∼23. 6℃ at St. 7 and the difference between two stations, was 3.3∼6.6℃, when, the temperature at St. 5∼7 was higher than 1∼4. Transparency was 3.2m at St. 7, 11m at St, 3 and salinity was 29, 9∼32.2% at St. 1∼4, 28.4∼29.7% at 5∼7. The surface water of dissolved oxygen content was 5. 3∼6.2cc/ℓ, and bottom water was 5.3∼5.9cc/ℓ. The content of C.O. D was 0.49∼1.2ppm and it was higher in April than it was in May and June. In case of NO2-N, the surface water was 0.3∼0.6㎍- at/ℓ, bottom water was 0.3∼0.6㎍- at/ℓ, and it's contents was the highest in June. The contents of PO4- P was 0.03∼0.0㎍- at/ℓ, SiO2 was 5.9∼10.1㎍- at/ℓ and Ammonia-N was 0.8∼1.6㎍ at/ℓ.
김운중,천춘길,김영균 朝鮮大學校 建設技術硏究所 1999 建設技術硏究 Vol.19 No.2
This study provides the economical plan of transmission and distribution line in order to make the most effect investment to the waterworks for the stabilized water supply. When it come down to the planning of pipeline, it is profitable that the pipe line is located on-the center of the demand area with considering surveying of roads. And the result of the evaluation indicates that high-impact pipe, ductile cast iron pipe and steel pipe are the most desirable pipes. In the designing of the pipeline, the diameter should be selected with considering of the regional condition and water consumption. It was found that the variation of maintenance-cost with increasing of pumping and electric supply is more important factor of the total-cost that the variation of construction-cost. The strategy of system for the stabilized water supply must be based on the double conveying pipe line, the searching for the headwaters, the use of recycling water at wastewater treatment plant, the improvement of the decrepit waterworks and system development and automation plan.
혈장내 testosterone정량을 위한 화학발광 면역분석법 개발
박관하,김영길,우종규,최상훈,최민순 群山大學校 1997 論文集 Vol.24 No.-
The immunoassay monitored by the detection of chemiluminescence for the determination of plasma testosterone has been developed. The labelled antigen was testosterone-3-carboxymethyl oxime-aminobutylethyl isoluminol. Following an antigen-antibody reaction, the bound fraction was separated from free fraction by the addition of dextran-coated charcoal followed by a centrifugation. Sodium hydroxide (5N) was added and the mixture incubated for 1hr at 60℃. Luminescence is initiated by oxidation of the label with microperoxidase-hydrogen peroxide and the signal integrated for 10sec. The light yield was inversely proportional to the concentration of testosterone in the standard of samples. This method appeared to show a similar sensitivity, specificity, precision, and an accuracy compared to the conventional radioimmunoassay(RIA) with a tritiated antigen. The results obtained from the analysis of plasma testosterone were in good agreement with the values derived by RIA(γ=0.97)