Isolation and Characterization of a Weizmannia coagulans Bacteriophage Youna2 and Its Endolysin PlyYouna2

Son Bokyung,Kim Youna,Yu Booyoung,Kong Minsuk 한국미생물·생명공학회 2023 Journal of microbiology and biotechnology Vol.33 No.8

Weizmannia coagulans (formerly Bacillus coagulans) is Gram-positive, and spore-forming bacteria causing food spoilage, especially in acidic canned food products. To control W. coagulans, we isolated a bacteriophage Youna2 from a sewage sludge sample. Morphological analysis revealed that phage Youna2 belongs to the Siphoviridae family with a non-contractile and flexible tail. Youna2 has 52,903 bp double-stranded DNA containing 61 open reading frames. There are no lysogenyrelated genes, suggesting that Youna2 is a virulent phage. plyYouna2, a putative endolysin gene was identified in the genome of Youna2 and predicted to be composed of a N-acetylmuramoyl-L-alanine amidase domain (PF01520) at the N-terminus and unknown function DUF5776 domain (PF19087) at the C-terminus. While phage Youna2 has a narrow host range, infecting only certain strains of W. coagulans, PlyYouna2 exhibited a broad antimicrobial spectrum beyond the Bacillus genus. Interestingly, PlyYouna2 can lyse Gram-negative bacteria such as Escherichia coli, Yersinia enterocolitica, Pseudomonas putida and Cronobacter sakazakii without other additives to destabilize bacterial outer membrane. To the best of our knowledge, Youna2 is the first W. coagulans-infecting phage and we speculate its endolysin PlyYouna2 can provide the basis for the development of a novel biocontrol agent against various foodborne pathogens.

Effects of Mother-Nurse Partnership on Parenting Stress and Family Resilience : Congenital Heart Disease Child’s Mother

Youna Chang,Jiyoung Lim,Hye Won Yoon 한국간호과학회 2023 한국간호과학회 학술대회 Vol.2023 No.11

Increase in Discharge Capacity of Li Battery Assembled with Electrochemically Prepared V2O5/polypyrrole-composite-film Cathode

Youna Kim,Joo-Seong Kim,Minh-Triet Thieu,Hung-Cuong Dinh,여인형,조원일,모선일 대한화학회 2010 Bulletin of the Korean Chemical Society Vol.31 No.11

Flexible composite films of V2O5 and conductive polypyrrole (V2O5/PPy) were grown by facile electrochemical polymerization, wherein an anodization potential was applied to the substrate electrode in an electrolyte solution containing pyrrole monomer and dispersed V2O5 particles. The coating of polypyrrole (PPy) on the surface of V2O5particles was induced by the oxidative catalytic action of V2O5 during the electrochemical polymerization of pyrrole. PPy in the composite film connects the isolated V2O5 particles. This results in the formation of conductive networks in the composite film cathode, thereby enhancing the Li+ ion diffusion to the surface of the isolated V2O5 particles and thus increasing the accessibility of the Li+ ions. The specific capacity tests of the Li rechargeable batteries revealed that the discharge capacity of this composite film cathode was higher, i.e., 497 mAhg-1, than that of V2O5/PPy powder or pristine V2O5.

Enzyme-free colorimetric assay of uracil DNA glycosylase activity based on toehold-mediated DNA strand displacement event

Youna KIM,Yeonkyung PARK,Chang Yeol LEE,Hyun Gyu PARK 한국생물공학회 2017 한국생물공학회 학술대회 Vol.2017 No.10

Clinical Characteristics and Outcomes of Mothers and Newborn Babies at an Integrated Perinatal Center for High-risk Pregnancy and Neonatal Care

( Youna Park ),( Mi Hyeon Gang ),( Yong Wook Lee ),( Byung Hun Kang ),( Min A Lee ),( Mea-young Chang ) 대한주산의학회 2020 Perinatology Vol.31 No.4

Objective: The appropriate perinatal care and safe delivery of high-risk pregnant women have a significant impact on the prognosis of newborn. The purpose of this study is to analyze the characteristics and outcomes of high-risk pregnant women and their babies in a single integrated care center for high risk pregnancy and neonate care. Methods: The subjects were babies born in Chungnam national university hospital, their mothers and transferred babies between January 1, 2018 and December 31, 2018. We analyzed the time of transfer, interval between transfer and delivery, clinical features in pregnant women and their babies. The clinical characteristics and outcomes of the babies were compared to those of outborn babies and analyzed according to the timing of the transfer and duration between time of the mother's transfer and delivery. Results: Mean gestational age at first visit was 26.4 weeks. The average time to delivery after the first visit was 73.1 days. The main reasons for the mother's transfer were fetal problems (17.0%), obstetric and other underlying diseases of mothers (18.9%) and various problems related to pregnancy (52.4%). Babies born in hospital had lower gestational age and birth weight than transferred babies and their main reason for admission was prematurity (65.5%). The earlier transfer of mothers and longer interval between transfer and delivery were related to lower rate of emergency cesarean section in mothers, lower admission rate and larger gestational age and birth weight in babies. Conclusions: The early transfer of high-risk pregnant women and their appropriate management can optimize the outcomes of babies as well as mothers.

Chronic Musculocutaneous Nerve Injury: An Important Differential in Progressive Arm Atrophy

Younas Umer,Khalil Muhammad Tawab,Yasmeen Rehana,Shafiq Omar,Irshad Imran 대한근전도전기진단의학회 2022 대한근전도 전기진단의학회지 Vol.24 No.3

An isolated musculocutaneous nerve (MCN) injury is a rare condition that can be easily missed if it presents late. A 28-year-old man reported painless and progressive wasting of the right arm for 6 months. On examination, there was visible wasting of the right biceps brachii muscle along with its slight weakness, depressed biceps jerk, and an impaired pinprick sensation in the lateral antebrachial cutaneous nerve distribution. He described a history of a road traffic accident 14 months beforehand. Based on the history and clinical examination, the differential diagnosis included an isolated MCN injury, upper trunk plexopathy, lateral cord plexopathy, C5/6 radiculopathy, and monomelic amyotrophy involving the C5/6 myotomes on the right side. The results of nerve conduction studies and electromyography were consistent with chronic proximal MCN neuropathy (right). In cases of arm wasting without pain or numbness, MCN injury should be included in the differential diagnosis, even in the presence of good power of the elbow flexors. The importance of detailed history-taking and clinical correlation cannot be over-emphasized in such cases.

Blossom smart expander technology for tissue expander-based breast reconstruction facilitates shorter duration to full expansion: A pilot study

Youna K. Choi,Danielle H. Rochlin,Dung H. Nguyen 대한성형외과학회 2020 Archives of Plastic Surgery Vol.47 No.5

Background This study evaluated the Blossom system, an innovative self-filling, rate-controlled, pressure-responsive saline tissue expander (TE) system. We investigated the feasibility of utilizing this technology to facilitate implant-based and combined flap with implant-based breast reconstruction in comparison to conventional tissue expansion. Methods In this prospective, single-center, single-surgeon pilot study, participants underwent either implant-based breast reconstruction or a combination of autologous flap and implantbased breast reconstruction. Outcome measures included time to full expansion, complications, total expansion volume, and pain scores. Results Fourteen patients (TEs; n=22), were included in this study. The mean time to full expansion was 13.4 days (standard error of the mean [SEM], 1.3 days) in the combination group and 11.7 days (SEM, 1.4 days) in the implant group (P=0.78). The overall major complication rate was 4.5% (n=1). No statistically significant differences were found in the complication rate between the combination group and the implant group. The maximum patient-reported pain scores during the expansion process were low, but were significantly higher in the combination group (mean, 2.00±0.09) than in the implant group (mean, 0.29±0.25; P=0.005). Conclusions The reported average duration for conventional subcutaneous TE expansion is 79.4 days, but this pilot study using the Blossom system achieved an average expansion duration of less than 14 days in both groups. The Blossom system may accommodate single-stage breast reconstruction. The overall complication rate of this study was 4.5%, which is promising compared to the reported complication rates of two-stage breast reconstruction with TEs (20%–45%).

Impacts of presenteeism on work-related injury absence and disease absence

Youna Won,Hwan-Cheol Kim,Jiho Kim,Minsun Kim,Seong-Cheol Yang,Shin-Goo Park,Jong-Han Leem 대한직업환경의학회 2022 대한직업환경의학회지 Vol.34 No.-

Background: Many studies have been conducted on presenteeism and absenteeism, but the relationship between presenteeism and absenteeism is unclear. This study was undertaken to investigate the effect of presenteeism on future absenteeism. Methods: This study was conducted on non-white collar workers that underwent general health examinations in 2014 and 2015. We asked subjects to fill out a questionnaire about their experiences of presenteeism for the past one year in 2014. In 2015, it was checked whether the same subjects had any experience of absence from work due to injury or disease during the past year. The χ² test was used to analyze relationships between sociodemographic and occupational characteristics, presenteeism, and absence. Odds ratios (ORs) were calculated by logistic regression analysis controlled for confounding factors. Results: A total of 12,572 workers were included in the study. For workers who experienced presenteeism, the OR for injury absence was 2.705 (95% confidence interval [CI]: 2.044–3.581), and the OR for disease absence was 4.906 (95% CI: 3.996–6.024) after adjusting for sociodemographic and occupational confounding factors. For men that experienced presenteeism, the OR for injury absence was 3.035 (95% CI: 2.258–4.081), and the OR for disease absence was 5.508 (95% CI: 4.340–6.989). For women that experienced presenteeism, the OR for injury absence was 1.322 (95% CI: 0.577–3.028), which was not significant, and the OR for disease absence was 3.629 (95% CI: 2.405–5.475). Conclusions: This study suggests that presenteeism can influence future absenteeism. The effect of presenteeism may depend on cause of absence. Men who experienced presenteeism showed greater effects on injury and disease absence than women. For women, experience of presenteeism had a significant effect on disease absence but not on injury absence.

Enzyme-free and Label-free Colorimetric Detection of Uracil DNA Glycosylase Activity Based on Toehold-Mediated DNA Strand Displacement Circuit

Youna KIM,Jinhwan LEE,Hyun Gyu PARK 한국생물공학회 2019 한국생물공학회 학술대회 Vol.2019 No.10

Chromosome numbers and meiotic behavior in some species of Asteraceae from high altitudinal regions of Kashmir Himalayas

Younas Rasheed Tantray,Ishrat Jan,Mohammad Saleem Wani,Vijay Kumar Singhal,Raghbir Chand Gupta 국립중앙과학관 2021 Journal of Asia-Pacific Biodiversity Vol.14 No.4

Disparity or continuity in the chromosome count within taxa of various categories has been verified to bean imperative character for taxonomic classification. Male meiotic studies in 64 species (161 accessions)of composits from geographically isolated regions were examined in detail in order to update the existingknowledge of meiotic chromosome numbers of composits from the outer part of the Himalayas. Myriactisjavanica (2n ¼ 36) and Tanacetum nubigenum (2n ¼ 36) have been counted chromosomally for the firsttime at the world level. Besides, Koelpinia linearis (2n ¼ 36), Ajania fruticulosa (2n ¼ 18), Crepis flexuosa(2n ¼ 14), and Tanacetum gracile (2n ¼ 18) have been analyzed chromosomally for the first time fromIndia. New intraspecific diploid/polyploid cytotypes are added for Artemisia desertorum (2n ¼ 6x ¼ 54),Carduus nutans (2n ¼ 4x ¼ 16), Erigeron annuus (2n ¼ 2x ¼ 18), Leontopodium jacotianum (2n ¼ 4x ¼ 48),Pentanema indicum (2n ¼ 6x ¼ 54), Scorzonera virgata (2n ¼ 2x ¼ 16), and Tragopogon pratensis(2n ¼ 2x ¼ 12). Besides, eight species have been evaluated cytologically for the first time from KashmirHimalayas. Various meiotic abnormalities like cytomixis, structural heterozygosity, chromatin stickiness,nonsynchronous disjunction, laggards, bridges, unoriented chromosomes leading to pollen sterility havebeen observed for the first time in some species. The presence of B-chromosomes has been detected inAchillea millefolium (2n ¼ 18þ0-1B) and Saussurea candolleana (2n ¼ 26þ0-1B). Among meioticallyanalyzed species, diploids (76.5%) and polyploids (23.5%) prevail.