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구내 표준 방사선사진을 이용한 구내방사선촬영기의 선질 평가
이상섭,권혁락,심우현,오승현,이지연,전국진,김기덕,박창서 대한구강악안면방사선학회 2000 Imaging Science in Dentistry Vol.30 No.3
Purpose : This study was to evaluate the beam quality of intraoral X-ray equipments used at Yonsei University Dental Hospital (YUDH) using the half value layer (HVL) and the characteristic curve of intraoral standard X-ray film. Materials and Methods : The study was done using the intraoral X-ray equipments used at each clinical department at YUDH. Aluminum filter was used to determine the HVL. Intraoral standard film was used to get the characteristic curve of each intraoral X-ray equipment. Results : Most of the HVLs of intraoral X-ray equipments were higher than the least recommended thickness, but the REX 601 model used at the operative dentistry department and the X-707 model used at the pediatric dentistry department had HVLs lower than the recommended thickness. The slopes of the characteristic curves of films taken using the PANPAS 601 model and REX 601 model at operative dentistry department, the X-705 model of prosthodontic dentistry department, and the REX601 model at the student clinic were relatively low. Conclusion : HVL and the characteristic curve of X-ray film can be used to evaluate the beam quality of intraoral X-ray equipment. In order to get the best X-ray films with the least radiation exposure to patients and best diagnostic information in clinical dentistry, X-ray equipment should be managed in the planned and organized fashion. (Korean J Oral Maxillofac Radiol 2000; 30: 183-188)
An FMN-containing NADH-quinone reductase from streptomyces sp
Youn, Hong-Duk,Lee, Jin-Won,Youn, Hwan,Lee, Jeong-Kug,Hah, Yung-Chil,Kang, Sa-Ouk The Microbiological Society of Korea 1996 The journal of microbiology Vol.34 No.2
NADH-quinone reductase was purified 22-fold from the cytosolic fraction of Streptomyces sp. Imsnu-1 to apparent hemogenity, with an overall yield of 9%, by the purification procedure consisting of ammonium, sulfate precipitation and DEAE Sephacryl S-200 and DEAE 5 PW chromatographies. Thes molecular mass of the enzyme determined by gel filtration chromatography was found to be 110 kDa. SDS-PAGE revealed that the enzyme consists of two sugunits with a molecular mass of 54 kDa. The enzyme contained 1 mol of FMN per subunit as a cofactor. The $A_{272}$ A$_{457}$ ratio was 6.14 and the molar extinction coefficients were calculated to be 20, 800 and 25, 400M$^{-1}$ $cm^{-1}$ / AT 349 AND 457 nm, respectively. The N-terminal sequence of the enzyme contained the highly conserved fingerprint of ADP-binding domain. The enzyme used NADH as an electron donor and various quinones as electron acceptors. Cytochrome c was practically inactive. Air-stable flavin semiquinone was produced by the addition of NADH to the enzyme. Also, naphthosemiquinone was detected in the reaction mixture containing the enzyme.
Sung Youn Heo,You Na Seo,Won Seok Ju,Hyun Gyu Lim,Sang-Young Seo,Jin Hyoung Cho,Park Soon Ju,Young-Kug Choo 한국당과학회 2018 한국당과학회 학술대회 Vol.2018 No.01
Mesenchymal stem cells are undifferentiated stem cells that can differentiate into various cells and tissues. In this study, we confirmed the difference in expression and differentiation rate of gangliosides during the differentiation of mini-pig adipose-derived mesenchymal stem cells into neuronal cells using secretion medium from PMA-induced human macrophage-like cell line U937 (Macro SM). We showed that nm23, one of several proteins secreted in the culture medium of activated human macrophages, decreased the neuronal differentiation rate of mesenchymal stem cells derived from mini-pig adipose, and inhibited the function and the synthesis enzyme of GD3. In conclusion, one of the factors that decreased the differentiation rate of mini-pig adipose-derived mesenchymal stem cells into neuronal cells by Macro SM is the nm23 protein.
Sung Youn Heo,Dong Hoon Kwak,Yu Na Seo,Won Seok Ju,Hyun Gyu Lim,Seo Yi Lee,Ji-Su Kim,Kyu-Tae Chang,Young-Kug Choo 한국당과학회 2016 한국당과학회 학술대회 Vol.2016 No.07
Mesenchymal stem cells can differentiate into multiple lineages depending on their exposure to differing biochemical and biomechanical inductive factors. The Ganglioside is included in biomarker, that are the major component of cytoplasmic cell membranes complex glycosphingolipids, and play a role in the control of biological processes. However, role of ganglioside in osteogenesis of mini-pig adipose derived mesenchymal stem cells (AD-MSCs) unclear . We have knocked down was ganglioside synthase UDP-glucose ceramide glucosyltransferase (UGCG)using a siRNA differentiation into osteoblast was markedly decreased in UGCG-knocked down AD-MSCs. The results showed significant inhibits alkaline phosphatase, osteocalcin, osteopontin, and Runt-related transcription factor 2 in UGCG knock down AD-MSCs compared to with the control AD-MSCs. Thus we guess, maybe ganglioside is essential factor to stimulate osteogenesis in AD-MSCs.
Sung Youn Heo,You Na Seo,Won Seok Ju,Hyun Gyu Lim,Sang-Young Seo,Jin Hyoung Cho,Park Soon Ju,Young-Kug Choo 한국당과학회 2018 한국당과학회 학술대회 Vol.2018 No.01
Macrophages, the cells of the innate immune system, play a key role in all three types of xenograft rejection. However, the mechanism underlying their direct effects is incompletely understood. To study the direct effects of macrophages, established human model system combined the U937 monocyte cell line and mini-pig adipose derived mesenchymal stem cells (mp AD-MSCs). Treatment of mp AD-MSCs with macrophage secretion medium (Macro SM) markedly reduced the cell viability, increased lactate dehydrogenase release, and induced apoptotic cell death. The proteomic analysis identified 17 proteins in Macro SM. Based on the gene ontology analysis tool, matrix metalloproteinase (MMP)-9 was selected as the protein affecting MSC viability. To confirm the effect of MMP-9, cells were treated with recombinant human MMP-9 that resulted in decreased cell viability and increased lactate dehydrogenase release. These results suggest that MMP-9 may decrease the cell viability by damaging the cell membrane in the co-culture model.
이국종 ( Kug Jong Lee ),김재용 ( Jae Yong Kim ),이강현 ( Kang Hyun Lee ),서길준 ( Gil Joon Suh ),윤여규 ( Yeo Kyu Youn ) 대한외상학회 2005 大韓外傷學會誌 Vol.18 No.1
An ideal trauma care system would include all the components identified with optimal trauma care, such as prevention, access, acute hospital care, rehabilitation, and research activities. Central to an ideal system is a large resource-rich trauma center. The need for resources is primarily based on the concept of being able to provide immediate medical care for unlimited numbers of injured patients at any time. Optimal resources at such a trauma center would include inhouse board-certified emergency medicine physicians, general surgeons, anesthesiologists, neurosurgeons, and orthopedic surgeons. Other board-certified specialists would be available, within a short time frame, to all patients who require their expertise. This center would require a certain volume of injured patients to be admitted each year, and these patients would include the most severely injured patients within the system. Additionally, certain injuries that are infrequently seen would be concentrated in this special center to ensure that these patients could be properly treated and studied, providing the opportunity to improve the care of these patients. These research activities are necessary to enhance our knowledge of the care of the injured. Basic science research in areas such as shock, brain edema, organ failure, and rehabilitation would also be present in the ideal center. This trauma center would have an integrated concurrent performance improvement program to ensure optimal care and continuous improvement in care. This center would not only be responsible for assessing care delivered within its trauma program, but for helping to organize the assessment of care within the entire trauma system. This ideal trauma center would serve as a total resource for all organizations dealing with the injured patient in the regional area.
임상연구 : Sevoflurane 흡입마취유도 시 동맥압 변화의 기전 평가: Modelflow 방법을 이용한 혈역학 변수의 Beat-to-Beat 분석
김영국 ( Young Kug Kim ),윤미옥 ( Mi Ok Youn ),서형석 ( Hyung Seok Seo ),최재혁 ( Jae Hyuk Choi ),최병문 ( Byung Moon Choi ),신원정 ( Won Jung Shin ),도경준 ( Kyung Jun Do ),조선준 ( Sun Joon Cho ),강수진 ( Su Jin Kang ),전인구 ( 대한마취과학회 2006 Korean Journal of Anesthesiology Vol.51 No.1
Background: A modelflow method provides beat-to-beat analysis of cardiovascular variables based on arterial pulse pressure analysis. In this study, we assessed the mechanism of arterial blood pressure (ABP) change during sevoflurane induction by the analysis of beat-to-beat hemodynamic changes using a modelflow method. Methods: Beat-to-beat ABP was measured during a stable conscious state (baseline) and vital capacity induction with sevoflurane 6 vol% and oxygen 8 L/min in 18 healthy living liver transplant donors. Alterations of beat-to-beat systolic ABP, mean ABP, diastolic ABP, heart rate (HR), stroke volume (SV), cardiac output (CO), and total peripheral resistance (TPR) by sevoflurane induction were estimated noninvasively using a modelflow method simulating aortic input impedance from an ABP waveform. Results: After sevoflurane induction, ABP, SV, CO, and TPR decreased significantly (all P < 0.05), but HR did not change significantly. After tracheal intubation, ABP, CO, and TPR did not change significantly compared with baseline, but HR increased and SV decreased significantly (both P < 0.05). Conclusions: Using a modelflow beat-to-beat analysis of cardiovascular variables, we found that ABP did not change significantly compared to baseline after tracheal intubation during sevoflurane induction, indicating the counteraction of increased HR and decreased SV, and that the reduction of SV by tracheal intubation suggests the suppression by increased HR and TPR compared with that after sevoflurane induction. (Korean J Anesthesiol 2006; 51: 17~23)