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Kwang Youll Lee,공현기,이선우 한국식물병리학회 2012 Plant Pathology Journal Vol.28 No.4
Burkholderia pyrrocinia CH-67 is a biocontrol bacterium with strong antifungal activity against several plant pathogenic fungi. Transposon mutagenesis was performed to identify the genes responsible for the antifungal activity of B. pyrrocinia CH-67. Of the 2,500 mutants tested using the Fulvia fulva spore screening method, a mutant deficient in antifungal activity, M208, was selected. DNA sequence analysis of the transposon-inserted region revealed that a gene encoding an adenylate kinase-related kinase was disrupted in M208. Antifungal activity was restored in M208 when a full-length adenylate kinase gene with its promoter was introduced in trans. The deduced amino acid sequence of adenylate kinase from CH-67 was 80% identical to that of B. cenocepacia MCO-3. Adenosine diphosphate supplementation or high levels of adenosine triphosphate and adenosine monophosphate together restored antifungal activity in M208, suggesting that adenylate kinase of B. pyrrocinia CH-67 is involved in antifungal activity expression.
New and Improved Time-selective Self-triggering Water Sampler: AUTTLE
Jin, Jae-Youll,Hwang, Kuen-Choon,Park, Jin-Soon,Eo, Young-Sang,Kim, Seong-Eun,Yum, Ki-Dai,Oh, Jae-Kyung Korea Institute of Ocean Science Technology 2000 Ocean and Polar Research Vol.22 No.2
Time-selective self-triggering water sampler, AUTTLE developed by Jin et al. (1999) has been improved in order to prevent pre-deposition of suspended sediments (SS) before sampling. By using two solenoids, the improved sampler is able to be moored or deployed with inclination. Its position is changed to horizontal position by activating the first solenoid, and then the endcaps of the sampling bottle are closed by the second solenoid that is driven three times to minimize possible failure of sampling. An external control unit for setting sampling time has been also constructed. Additionally, the electric circuit housing of the sampler has been modified to be detached from the sampling bottle when operating manually. Its performance has been confirmed through flume tests and a field experiment. It will serve as a valuable tool in the various fields of oceanography and environmental engineering, especially where seawater sampling synchronized at several sites and/or the information in storm period is important.