내산성 및 내담즙성이 우수한 양돈용 유산균의 선발

유자영,양시용,송민동 建國大學校 自然科學硏究所 2002 建國自然科學硏究誌 Vol.13 No.1

본 연구는 가축의 장내에서 유익한 영향을 주는 유산균을 돼지의 분변으로부터 분리하고 내산성과 내담즙성이 뛰어난 균을 선별하여 유산균 생균체를 준비하고자 실시하였다. 분변에 섞여 있는 다른 균들과 산이나 담즙액에 약한 균들은 처음부터 분리하기 위하여 pH를 2.0으로 맞춘 MRS broth에 배양시키고 oxgall이 첨가된 배지에 도말하여 40개의 균주를 분리하였다. BCP배지를 이용해서 유산의 생성여부를 확인하고, O.D값과 pH를 측정하였으며, 그람염색과 형태관찰을 통하여 33개의 균주를 분리하였다. 분리된 균주중에서 산에 대한 내성이 있다고 판단되는 12개의 균주를 분리해냈고, 12개의 균주의 내산성 및 내담즙성을 3회에 걸쳐 시험하고 내산성과 내담즙성이 우수한 3개의 균주를 선별하였다. 마지막으로 담즙산 농도별 시험을 행함으로써 가축의 장까지 살아서 도달하기 위해 꼭 갖추어야 할 조건인 내산성과 내담즙성이 가장 좋은 #32-1 균주를 최종 선발하였다. 유산균이 생균제로서의 기능을 수해하려면 oxgall이 0.3% 함유된 배지에서 성장할 수 있을 정도의 내성을 갖고 있어야 한다는 보고가 있는데 #32-1 균주의 경우 담즙액의 농도가 3%인 배양액에서도 높은 생존률을 보임으로써 생균제로서 사용하기위해 갖고 있어야 할 담즙에 대한 내성이 우수하다고 판단하였다. This study was performed to screen lactic acid bacteria for the direct-fed-microbials. A lactic acid bacteria which has resistant to acid and bile salts were isolated from swine feces. Among the previously obtained acid tolerant strains, three of them show resistant to artifical gastric juice and bile. Finally, 321- stain was selected for good growth an high survival rate. The finally selected 32-1 stain was identified as Lactobacillus salivarius. The strain, which named L. salivarius JY 32-1, was able to tolerate acid condition down to pH 2.5 and could also tolerate bile at 3%.

나경석의 '생산증식'론과 물산장려운동

유시현 역사문제연구소 1997 역사문제연구 Vol.- No.2

전자거래를 위한 패키지 표면디자인에 관한 연구

유시천,윤찬종 한국패키지디자인학회 2001 상품문화디자인학연구 Vol.10 No.-

From agriculture society to industrial society and currently progressing through information intellectual society, these stages of processes developed into the information technology. Which means it's for different environment than using the Internet communication line to get an information and purchasing products. Due to the information society the Internet became the central axis and human beings more rapid, more convenience, and Electronic Transaction are so brisk that product's Brand-package-Design has become more and more important. In the Electronic Transaction, Brand package design is consider same in general trade, and it's inexpensive way to protect the trade mark and considering distinctiveness that consumers are purchasing through picture which means on line, so that picture image should be updated for trade mark and package and it's the active role of the design following the information society. A successful Electronic Transaction is using the mass media to advertising for publicizing promotion membership, and events which has been considered usual practices but beyond that there's packing which should not be has pass over. Now a boundless development and competition of Electronic Transaction has been in progress repeatedly. In an information society of Twenty-First century. Let's be the best Electronic Transaction era.

男女大學生의 結婚觀(I) : 그들의 意識과 行動을 中心으로

柳時中,韓有常 慶北大學校 東洋文化硏究所 1984 東洋文化硏究 Vol.11 No.-

In human society, family is an institution and a universal group, and generally based upon marriage and bloodrelation. The existence of family in society presupposed the marriage, through which a family is established. It is known that the marriage institution have been changed by the ideological and cutural variable as well as social and economic ones through human history. Through the process of modernization, Korea has undergone various change in every social aspect. Especially in institutional and actual aspects, the family and the marriage have been changed a great deal by the rapid urbanization and industrialization for about 40 years, and these changes have been investigated scientifically. In scientific researches on the social phenomena, testifying hypothesis based on certain sociological facts and its theoretical construction are freat importance, and so is the acculuation of the fact-findings in which variousfacts about specific social phenomena are concretely described. Marriage is, as said above, an institutional combination of both sexes, especially of young men and women. In the given conditions of Korean society, adolescents who reach their marriageable age are, for the most part, young workers and college students, though. They show a tendency to select their spouses and marry homogamously, and this tendency is true of both the college undergraduates and the graduates. For example, they trand to select their spouses of the same age and on the same level of education. But these homogamous tendencies are not always the same in their details and categories. They have changed according to the times and societies, instead. Because of the differences of gender, the man and woman college students, although they have grown up in the same social, economic and cutural surroundings, don't have the same views of marriage and can't keep the pace with each other in their practical behaviors. After analyzing the man and woman college students of the graduating class in Taegu regions who have been influenced by the radical social changes, this paper is written, in accordance with the procedures of sociological research, 1) to study their general consciousness and behavior connected with marriage, 2) to show the differeces in consciousness and behavio between the man and woman college students, and 3) to compare the college students' tendencies in marriage in Korea with those in Europe and America, espacially with those in Japan which has been under the influences of Confucianism and modernized prior to us. The topics dealt with in this study covers 1) how the acquaintance of the other sexes is made before marriage 2) how and by whom the selection of a spouse is determined 3) what the criteria on his selection are. Findings on the sex problems and life plannings after marriage will be presented in other journals.

男女大學生의 結婚觀(II) : 그들의 意識과 行動을 中心으로

柳時中,韓有常 경북대학교 사회과학대학 1984 社會科學 Vol.3 No.-

The purpose of this study is to examine some attitudes that college students assume toward marriage. In the article "College Students' Attitudes Toward Marriage: Their Spouse Selection Routes and Criteria" (Yu and Han, 1984) to appear in Oriental Culture Research, vol.11 (1984), the authors have advanced an analysis of prevailing spouse selection routes and criteria. As a sequel to Yu and Han (1984), this study tries to investigate problems concerning premarital sexual intercourse and postnuptial life planning that enter into the spouse selection process. Since Korea began to modernize itself, diverse changes have occurred in our society. Particularly, the Western free-sex trend seems to have continued its infiltration to such an extent that college students in Korea are seriously affected by the mood. Upon investigation, however, it has been found out that most college students still impart a high value to premarital virginity, and that only a small number of students are actually experiencing premarital coitus. Questionaires about postnuptial life planning were employed to analyze the attitudes that college students take toward independent family life, residence problem, double-income family, and family planning. The result was that the nuclear and double-income family was more favored by female students. It also turned out that, regardless of sex, most college students hoped to find their postnuptial residence in cities and to have fewer children than before.

이행성 협심증 환자에서 운동부하 심전도 검사

이정우,박형서,박용규,노상필,이유선,정승현,김보영,이재환,최시완,정진옥,성인환 충남대학교 의과대학 의학연구소 2003 충남의대잡지 Vol.30 No.1

목적 : 관상동맥의 경련에 의한 이형성 협심증환자에 있어서 운동부하 심전도 검사의 결과는 매우 다양하다. 이에 저자들은 충남대학교병원 순환기내과에서 관상동맥조영술상 이형성 협심증으로 확진된 환자를 대상으로 운동부하 심전도 검사를 시행하여 이에 대한 결과를 연구하였다. 방법 : 1995년 1월부터 2002년 3월까지 흉통을 주소로 충남대학교병원 순환기 내과에 입원하여 관상동맥조영술을 시행하여 관상동맥의 내경이 50% 미만의 협착이 있고, 에르고노빈(ergonovine) 유발검사도 이형성 협심증으로 진단을 받은 233명중 운동부하 심전도 검사를 시행 받은 183명의 환자를 연구 대상으로 하였다. 운동부하 심전도 검사 결과의 판독시 발생할 수 있는 오류를 줄이기 위해서 운동부하 심전도 검사를 시행하기전 심전도상 ST분절의 상승(5예)이나 하강(8예)이 있는 경우 EH한 T파의 역위(19예)가 있는 32명의 환자를 제외한 총 151명의 환자를 대상으로 하였다. 운동부하는 Marquette사의 case 15 답차를 이용하였고, Bruce protocol에 따라 증상이 나타날 때까지 최대로 실시하였으며 각 stage 및 운동후의 혈압을 측정하고 12 유도 심전도를 기록하였다. 결과 : 1) 임상적 특성 - 내원시 임상 상은 안정형 협심증이 39예(25.8%), 불안정형 협심증이 103예(68.2%), 심근경색증이 9예(6.0%)이었다. 운동부하 심전도 검사 결과 양성 군과 음성군간에 있어서 당뇨병, 고혈압, 비만도, 총 혈청콜레스테롤치, 현재의 흡연 여부, 병력상 운동시 흉통의 유무, 내원시 임상적 진단, 협착의 정도, 연축의 수 등을 조사하였는 바 어떠한 인자들도 유의한 차이를 발견할 수 없었다. 2) 운동부하 심전도 검사 - 운동부하 심전도 검사를 시행 받은 151명의 환자중 음성인 경우는 134예(88.8%)이었고, 양성인 경우는 17예(11.2%)이었다. 양성 소견을 보인 환자들중 ST 분절의 상승이 4예(2.6%), ST 분절의 하강이 13예(8.6%)이었다. 3) 관상동맥조영술 - 관상동맥조영술상에서 혈관 경력 위치는 우관상동맥이 70예(46.1%), 좌전하동맥이 44예(28.9%), 좌회선동맥이 17예(11.2%)의 순으로 나타났다. 운동부하 심전도 검사상 ST 분절의 상승이 있었던 예중 Ⅱ, Ⅲ, aVF에서 ST 분절의 상승이 있었던 1예는 관상 동맥조영술상 우관상동맥에서 경련이 발생하였고, V2-V4에서 ST 분절의 상승이 있었던 2예중 관상동맥조영술상에도 좌전하행동맥에서 경련이 나타난 예는 1예이었으며 다른 1예는 좌회선동맥에서 경련이 나타났고, V5-V6에서 ST 분절의 상승이 있었던 1예는 관상동맥조영술상에도 좌회선동맥에서 경련이 나타났다. 운동부하 심전도 검사상 V5 또는 V6에서 ST 분절의 하강이 있엇던 13예중 6예(46.1%)는 관상동맥조영술상 우관상동맥에서 경련이 발생하였고, 3예(23.1%)는 좌전하행동맥에서, 1예(7.7%)는 좌회선동맥에서, 나머지 3예(23.1%)는 2개의 혈관이나 기타 분지에서 경련이 발생하였다. 결론 : 1) 이형성 협심증 환자는 운동부하 심전도 검사에서 11.2%의 양성소견을 보였다. 이중 ST 분절의 상승은 2.6%이었고, ST 분절의 하강은 8.6%이었다. 2) 이형성 환자중 운동부하 심전도 검사를 실시하여 음성을 보이는 경우 다혈관 관상동맥질환이기 보다는 이형성 협심증일 가능성이 더 높다고 예측할 수 있을 것으로 생각된다. 3) 이형성 협심증 환자중 운동부하 심전도 검사를 실시하여 ST 분절의 상승을 보이는 경우 다혈관 관상동맥질환이기 보다는 이형성 협심증일 가능성이 더 높다고 예측할 수 있을 것으로 생각된다. 4) 운동부하 심전도 검사 결과에 대한 당뇨병, 고혈압, 비만도, 총 혈청콜레스테롤치, 현재의 흡연여부, 병력상 운동시 흉통의 유무, 내원시 임상적 진단, 협착의 정도 등 어떠한 인자들도 3그룹(ST 분절의 상승, ST 분절의 하강, 음성)간에 유의한 차이를 발견할 수 없었다. 5) ST 분절의 상승 부위와 관상동맥조영술상 관상동맥의 연축이 일어나는 혈관과의 상관관계는 매우 높았다. No reports in the literature describe the results of exercise testings in a large number of patients with pure variant angina(coronary stenosis <50%) in Korea. In this report, We present the results of treadmil exercise testing in 151 patients with variant angina. 151 patients with angiographically proven coronary artery spasm underwent a treadmil exercise test. The clinical characteristics of variant angina patients classified according to ST-segment response to exercise were analyzed. Of 151 patients underwent a treadmil exercise test, negative result was seen in 134 patients(88.8%) and positive result was in 17 patients(11.2%). Of 17 patients saw positive result, exercise-induced ST segment elevation was present in 4 patients(2.6%) and ST segment depression was seen in 13 patients(8.6%). There was not a significant relationship between the ST segment response to exercise and the clinical variables(diabetes, hypertension, obesity, total cholesterol, current smoking, effort angina, clinical diagnosis, and degree of stenosis) assessed. Of 4 patients with ST segment elevation in treadmil exercise test, 1 patient with ST segment elevation in Ⅰ,Ⅲ,aVF had spasm in right coronary artery(100%) on coronary angiography, of 2 patients in V2-V4 had spasm in left anterior descending coronary artery in 1 patient(50%) and 1 patient in V5-V6 had spasm in left circumflex artery(100%). Positive treadmil exercise test was present in 11.2% of variant angina patient. If we have negative treadmil exercise result in patients with clinical manifestation of unstable angina at admission, we may have a suspicion of variant angina rather than multi-vessel disease. If we have exercise-induced ST segment elevation in patients with clinical manifestation of unstable angina at admission, we have a suspicion of variant angina rather than multi-vessel disease. Our result suggests that the correlation between the site of the ST segment elevation and the artery involved is quite good.

External cephalic version with epidural analgesia

( You Jung Shin ),( Dong-wook Kwak ),( You-jung Han ),( Si-won Lee ),( Min-hyoung Kim ),( Jin-hoon Chung ),( June-seek Choi ),( Hyun-kyeong Ahn ),( Hyun-mee Ryu ),( Moon-young Kim ),( Jung Yeol Han ) 한국모자보건학회 2015 한국모자보건학회 학술대회 연제집 Vol.2015 No.2

A study on Electron Heating Mechanism in RF Capacitively Coupled Plasma at Low Discharge Current based on PIC-MCC simulation

Si Jun Kim,Jang Jae Lee,Young Seok Lee,Chul Hee Cho,In Ho Seong,Han Sol Choi,Jin Ho Lee,Won Nyeong Jeong,Ha Jeong Choi,Ye Bin You,Min Su Choi,Shin Jae You 한국진공학회 2021 한국진공학회 학술발표회초록집 Vol.2021 No.2

This presentation presented analysis of the electron heating mechanism in capacitively coupled plasma based on particle-in-cell Monte Carlo collision (PIC-MCC) simulation. As discharge current decreases, abrupt reduction of low energy electron population in electron energy probability function(EEPF) were observed, while the gradual decrease of high energy electrons. This change of the EEPF shape was analyzed in terms of power absorption and electric filed. It is believed that this research elucidates the understanding of the electron heating mechanism at low discharge current

miR-1246 inhibits NFATc1 phosphorylation and regulates Th17 cell activation in the pathogenesis of severe alopecia areata

Si-si Qi,Ying Miao,You-yu Sheng,Rui-ming Hu,Jun Zhao,Qin-ping Yang 대한피부과학회 2023 Annals of Dermatology Vol.35 No.1

Background: We found microRNA (miR)-1246 to be significantly differentially expressedbetween severe active alopecia areata (AA) patients and healthy individuals. Objective: To explore the role and mechanism of miR-1246 in severe AA. Methods: Expression of miR-1246, dual-specific tyrosine phosphorylation-regulated kinase1A (DYRK1A), and nuclear factor of activated T cells 1c (NFATc1) in peripheral CD4+ Tcells and in scalp tissues of patients were detected using RT-qPCR, Western blot, and immunohistochemistryassays. Peripheral CD4+ T cells from the AA patients were transfectedwith lentiviral vectors overexpressing miR-1246. RT-qPCR and Western blot analysis wereused to measure mRNA or protein expression of retinoic-acid-receptor-related orphan nuclearreceptor gamma (ROR-γt), interleukin (IL)-17, DYRK1A, NFATc1, and phosphorylatedNFATc1. Flow cytometry was used to assay the CD4+IL-17+ cells proportion. ELISA wasused to measure cytokine levels. Results: miR-1246 levels decreased and DYRK1A and NFATc1 mRNA levels significantlyincreased in the peripheral CD4+ T cells and scalp tissues of severe active AA samples. NFATc1 protein expression was also significantly increased in the peripheral CD4+ T cellsbut not in the scalp tissues. NFATc1 positive cells were mainly distributed among infiltratinginflammatory cells around hair follicles. In peripheral CD4+ T cells of severe active AA,overexpression of miR-1246 resulted in significant downregulation of DYRK1A, NFATc1,ROR-γt, and IL-17 mRNA and phosphorylated NFATc1 protein, as well as a decrease in theCD4+IL-17+ cells proportion and the IL-17F level. Conclusion: miR-1246 can inhibit NFAT signaling and Th17 cell activation, which may bebeneficial in the severe AA treatment.

정보디자인 유형과 수준에 관한 연구

류시천 ( Si Cheon You ) 한국기초조형학회 2004 기초조형학연구 Vol.5 No.1