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메틸 5-히드록시 디나프로[1, 2-2'', 3'']푸란-7, 12 디온 6-카복시레이트의 미량분석
박유미(You Mie Park),장혜선(Hae Seon Jang),강경환(Kyoung Hwan Kang),김경님(Kyung Nim Kim),장성기(Seong Ki Jang),김박광(Bak Kwang Kim) 대한약학회 1993 약학회지 Vol.37 No.3
UV and high performance liquid chromatographic methods for the quantitative analysis of methyl 5-hydroxy-dinaphtho [1, 2-2'', 3''] furan-7,12-dione-6-carboxylate(MHDDC) in urine and blood were developed. The correlation coefficients of the calibration curves of MHDDC in chloroform, methanol and dioxane solution were 0.999, 0.997 and 0.998, respectively. MHDDC was resolved within 15 min and had a detection limit of 2~5ng at S/N 3 by using a reversed-phase column with two solvents (MeOH, HAc).
Conjugation of Ginsenoside Rg3 with Gold Nanoparticles
Park, You-Mie,Im, A-Rang,Joo, Eun-Ji,Lee, Ji-Hye,Park, Hyeung-Geun,Kang, Young-Hwa,Linhardt, Robert J.,Kim, Yeong-Shik Korean Chemical Society 2011 Bulletin of the Korean Chemical Society Vol.32 No.1
Ginsenoside Rg3 was reported to have important biological activities. We demonstrate conjugation and quantification procedures of ginsenoside Rg3 to gold nanoparticles for future biological and medical applications. Ginsenoside Rg3 was conjugated to spherical gold nanoparticles using a bifunctional heptaethylene glycol linker. The sulfhydryl group of heptaethylene glycol was adsorbed onto gold nanoparticles, and carboxylic acid end of heptaethylene glycol was bonded through a hydroxyl group of Rg3 via ester bond formation. The conjugation of Rg3 was characterized with various spectroscopic techniques, high resolution-transmission electron microscopy, and using Rg3 monoclonal antibody. The Rg3- functionalized gold nanoparticles were $4.7{\pm}1.0$ nm in diameter with a surface charge of -4.12 mV. The total number of Rg3 molecules conjugated to a 3.6 mL solution of gold nanoparticle was determined to be $9.5{\times}10^{14}$ corresponding to ~6 molecules of Rg3/gold nanoparticle. These results suggest that ginsenoside Rg3 is successfully conjugated to gold nanoparticles via heptaethylene glycol linker. The quantification was performed by using Rg3 monoclonal antibody without interference of gold's intrinsic color.
Roles of GSK3 in metabolic shift toward abnormal anabolism in cell senescence : Kim et al.
Kim, You-Mie,Seo, Yong-Hak,Park, Chan-Bae,Yoon, Soo-Han,Yoon, Gyesoon Wiley (Blackwell Publishing) 2010 Annals of the New York Academy of Sciences Vol.1201 No.1
<P>Abstract Diverse metabolic alterations, including mitochondrial dysfunction, have often been reported as characteristic phenotypes of senescent cells. However, the overall consequence of senescent metabolic features, how they develop, and how they are linked to other senescent phenotypes, such as enlarged cell volume, increased granularity, and oxidative stress, is not clear. We investigated the potential roles of glycogen synthase kinase 3 (GSK3), a multifunctional kinase, in the development of the metabolic phenotypes in cell senescence. The inactivation of GSK3 via phosphorylation is commonly observed in diverse cell senescences. Furthermore, subcytotoxic concentration of GSK3 inhibitor was sufficient to induce cellular senescence, accompanied by augmented anabolism, such as enhanced protein synthesis, and increased glycogenesis and lipogenesis, in addition to mitochondrial dysfunction. Anabolism was accomplished through glycogen synthase, eIF2B, and SREBP1. These metabolic features seem to contribute to an increase in cellular mass by increasing glycogen granules, protein mass, and organelles. Taken together, our results suggest that GSK3 is one of the key modulators of metabolic alteration, leading the cells to senescence.</P>