흰쥐 중추신경계내 난소로 투사하는 미주신경로에 관한 연구

김명주,장명세,고미희,노해숙,조해영,오문유,이봉희 濟州大學校 基礎科學硏究所 2001 基礎科學硏究 Vol.14 No.1

본 실험은 부교감신경의 하나인 미주신경이 난소를 지배하는 신경으로 관여하고 있는지를 pseudorabies 바이러스를 이용하여 난소신경로와 난소주사후 미주신경절단을 통하여 조사한 연구 보고이다. 이를 위하여 Sprague Dawley계 암흰쥐를 대상으로 pseudorabies 바이러스를 난소에 주사한 무리와 난소 주사후 미주신경을 절단한 무리에서 뇌를 적출하여 pseudorabies 바이러스에 대한 면역조직화학 염색을 시행하여 비교하였다. 본 실험결과 미주신경 중추신경로내의 상위신경핵들이 pseudorabied 바이러스에 대하여 양성반응이 줄어들었거나 관찰되지 않는 차이를 보였다. 즉 적색핵, 종말판혈관기관, 줄무늬체, 침상핵과 이마엽겉질은 부분적으로 난소의 미주신경로에 관여하고 있으며 미주신경등쪽핵, 고립로핵, 최후영역, 청색반점, 팔옆핵, 코리케퓨즈핵, 흑색질 및 시각교차위핵은 양성반응이 관찰되지 않아 미주신경으로 투사하는 부교감신경핵으로 조사되었다. The mammalian ovary has been known as receiving its innervation by sympathetic and sensory neurons of the peripheral nervous system from the brain. Recently, there were several functional reports that the vagus nerves were also regulating the ovarian function, but the vagus nerve had not been identified by clear morphological evidence. A viral transneuronal tracing technique has been used to demonstrate the morphological evidence for the central vagal involvement in ovarian innervation in brain areas. Bartha strain of pseudorabies virus injection was made into the ovary of Sprague Dawley rats. In experimental group, the vagus nerve of the same injection side was removed right after ovarian injection. At five days after initial injection, all the rats were sacrificed and brains were processed for immunohistochemistry. Several central nuclei including hypothalamic paraventricular nucleus showed strong bilateral positive labelings after unilateral injection in control rats, but the positive labelings were disappeared or decreased in several hypothalamic nuclei and nuclei of the vagus nerve. I n conclusion, these results provide the morphological evidence that vagus nerve has neural connection to ovary and by which the central nervous system may maintains the state of ovulation and reproduction as a possible parasympathetic routes in mammals.

Improvement on the Laminated Busbar of NPC Three-Level Inverters based on a Supersymmetric Mirror Circulation 3D Cubical Thermal Model

He, Feng-You,Xu, Shi-Zhou,Geng, Cheng-Fei The Korean Institute of Power Electronics 2016 JOURNAL OF POWER ELECTRONICS Vol.16 No.6

Laminated busbars with a low stray inductance are widely used in NPC three-level inverters, even though some of them have poor performances in heat equilibrium and overvoltage suppression. Therefore, a theoretical method is in need to establish an accurate mathematical model of laminated busbars and to calculate the impedance and stray inductance of each commutation loop to improve the heat equilibrium and overvoltage suppression performance. Firstly, an equivalent circuit of a NPC three-level inverter laminated busbar was built with an analysis of the commutation processes. Secondly, on the basis of a 3D (three dimensional) cubical thermal model and mirror circulation theory, a supersymmetric mirror circulation 3D cubical thermal model was built. Based on this, the laminated busbar was decomposed in 3D space to calculate the equivalent resistance and stray inductance in each commutation loop. Finally, the model and analysis results were put into a busbar design, simulation and experiments, whose results demonstrate the accuracy and feasibility of the proposed method.

초등학교 교사들의 태권도에 대한 인식

유승희(You, Seung-He),김은철(Kim, Eun-Chul) 경희대학교 스포츠과학연구원 2009 태권도과학 Vol.2 No.2

본 연구는 초등학교 교사들의 태권도에 대한 인식이 어떠한지를 알아보고 한국의 대표적인 문화인 태권도가 생활체육으로서의 역할 뿐만 아니라 초등학교 교육 속에서 나아가야할 방향을 모색하기 위하여 2008년 현재, 경기및 인천지역의 국․공립초등학교의 교사들을 모집단으로 설정한 후, 편의 표본 추출법으로 부천시와 인천시, 수원시, 용인시의 15개 국공립 초등학교에서 근무하는 교사, 총 340명을 설문조사하여 다음과 같은 결론을 얻었다.첫째, 성별에 따라 남교사가 여교사보다 태권도에 대한 인식이 높은 것으로 나타났고, 연령에 따라서는 35세~44세의 교사들보다 45세~54세, 55세 이상의 교사들이 흥미요인에서 높은 것으로 나타났다. 둘째, 담당학년에 따른태권도에 대한 인식의 차이는 고학년을 담당한 교사들이 저학년을 담당한 교사들보다 태권도의 무술적인 측면에대한 인식이 높은 것으로 나타났다. 셋째, 체육교과전담경력이 있는 교사들이 태권도에 대한 인식 중 무술인식요인이 높게 나타났다.넷째, 교직경력에 따라 태권도에 대한 인식요인의 차이가 있는 것으로 나타났다. The purpose of this study is to define objectively the primary school teacher's understanding of Taekwondo. As an object of this study, the researcher has set model group with the teachers of national public primary schools located in area of Gyeonggi-do and Incheon at present of 2008, and then, the researcher conducted the survey onto 340 teachers, who serve in 15 national public primary schools in Bucheon, Incheon, Suwon, and Yongin by using convenience sampling method. According to the population statistical characteristic of primary school teachers, it appeared that male teacher's understanding on Taekwondo is higher than that of female teachers, and also, according to ages, the teachers of 45-54 ages or over 55 age have much of interests on Taekwondo than teachers of 35-44 ages and the teachers in charge of high grade student have higher understanding in respect of military arts on Taekwondo than teachers in charge of low grade student. Together with this fact, many differences appeared in low grade reasons of understanding on Taekwondo according to the teaching experiences and between the teachers graduated the college of education and the teachers graduated the general university. Also many differences are appeared according to the participation or non-participation to sports for all or Taekwondo training experiences.

Improvement on the Laminated Busbar of NPC Three-Level Inverters based on a Supersymmetric Mirror Circulation 3D Cubical Thermal Model

Feng-You He,Shi-Zhou Xu,Cheng-Fei Geng 전력전자학회 2016 JOURNAL OF POWER ELECTRONICS Vol.16 No.6

Laminated busbars with a low stray inductance are widely used in NPC three-level inverters, even though some of them have poor performances in heat equilibrium and overvoltage suppression. Therefore, a theoretical method is in need to establish an accurate mathematical model of laminated busbars and to calculate the impedance and stray inductance of each commutation loop to improve the heat equilibrium and overvoltage suppression performance. Firstly, an equivalent circuit of a NPC three-level inverter laminated busbar was built with an analysis of the commutation processes. Secondly, on the basis of a 3D (three dimensional) cubical thermal model and mirror circulation theory, a supersymmetric mirror circulation 3D cubical thermal model was built. Based on this, the laminated busbar was decomposed in 3D space to calculate the equivalent resistance and stray inductance in each commutation loop. Finally, the model and analysis results were put into a busbar design, simulation and experiments, whose results demonstrate the accuracy and feasibility of the proposed method.

Modified Ferric Hydroxamate Spectrophotometry for Assaying Glycolic Acid from the Hydrolysis of Glycolonitrile by Rhodococcus sp. CCZU10-1

Yu-Cai He,You-Yan Liu,Cui-Luan Ma,Jian-He Xu 한국생물공학회 2011 Biotechnology and Bioprocess Engineering Vol.16 No.5

We successfully modified a ferric hydroxamate spectrophotometry method for assaying glycolic acid. Comparable to the high-performance liquid chromatography (HPLC)-based method, ferric hydroxamate spectrophotometry can be used to accurately monitor the time course of glycolonitrile bioconversion. Glycolic acid was assayed simply and rapidly at room temperature (25 ~35℃). Optimum culture conditions were obtained using this method to assay the glycolonitrile-hydrolyzing activity of Rhodococcus sp. CCZU10-1. The preferred carbon and nitrogen sources and ideal inducer were glucose (10 g/L),a composite of peptone (10 g/L) plus yeast extract (5 g/L),and ε-caprolactam (2 mmol/L), respectively. The optimal growth temperature and initial medium pH for Rhodococcus sp. CCZU10-1 glycolonitrile-hydrolyzing activity were 30℃ and pH 7.0. Modified ferric hydroxamate spectrophotometry could potentially be employed to assay other carboxylic acids.

PSSRC : A Web Service Registration Cloud Based on Structured P2P and Semantics

He, Qian,Zhao, Baokang,Chang, Liang,Su, Jinshu,You, Ilsun IGI Global 2016 International journal of data warehousing and mini Vol.12 No.2

<P>The Universal Description, Discovery and Integration (UDDI) registry is widely used for organizing Web services in the Internet, but it cannot meet the requirement of organizing massive Web services in the cloud computing environment. In this paper, a Web service registration cloud based on structured P2P and semantics (PSSRC) is presented. On the one hand, PSSRC works on a structured P2P overlay which is organized by using Pastry in multiple registration nodes, where each registration node is composed of seven modules namely system configuration, schedule and distribution, P2P communication, access and control, UDDI, resources monitoring, and semantic process. On the other hand, a semantic ontology database named WordNet is used to process semantic queries in PSSRC. PSSRC inherits the advantage of UDDI in that the registration and discovery of Web services are transparent to Web services providers. Furthermore, it is shown by experiment that the capacity of PSSRC can be extended dynamically, and both semantic queries and large scalable accesses are well supported.</P>

HMGB1 Promotes the Synthesis of Pro-IL-1β and Pro-IL-18 by Activation of p38 MAPK and NF-κB Through Receptors for Advanced Glycation End-products in Macrophages

He, Qiang,You, Hong,Li, Xin-Min,Liu, Tian-Hui,Wang, Ping,Wang, Bao-En Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.4

The high mobility group box-1 (HMGB1) protein and NALP3 inflammasome have been identified to play important roles in inflammation and cancer pathogenesis, but the relationships between the two and cancer remain unclear. The current study investigated the relationship between HMGB1 and the NALP3 inflammasome in THP-1 macrophages. HMGB1 was found unable to activate the NALP3 inflammasome and failed to induce the release of the IL-$1{\beta}$ and IL-18 in THP-1 macrophages. HMGB1 was also found significantly enhanced the activity of ATP to induce IL-$1{\beta}$ and IL-18 by the induction of increased expression of pro-IL-$1{\beta}$ and pro-IL-18. This process was dependent on activation of RAGE, MAPK p38 and NF-${\kappa}B$ signaling pathway. These results demonstrate that HMGB1 promotes the synthesis of pro-IL-$1{\beta}$ and pro-IL-18 in THP-1 macrophages by the activation of p38 MAPK and NF-${\kappa}B$ through RAGE. HMGB1 likely plays an important role in the first step of the release of the IL-$1{\beta}$ and IL-18, preparing for other cytokines to induce excessive release of IL-$1{\beta}$ and IL-18 which promote inflammation and cancer progression.

Anti-Inflammatory Activity of Ethanol Extract from Ainsliaea acerifolia

You-jeong Kim,He-li Hou,Ji hye Lee,Kuk young Jo,Taewan Kim 한국식품영양과학회 2015 한국식품영양과학회 학술대회발표집 Vol.2015 No.8

Effect of Garlic Oil on Fatty Acid Accumulation and Glycerol-3-Phosphate Dehydrogenase Activity in Differentiating Adipocytes

He, M.L.,Yang, W.Z.,You, J.S.,Chaves, A.V.,Mir, P.S.,Benchaar, C.,McAllister, T.A. Asian Australasian Association of Animal Productio 2009 Animal Bioscience Vol.22 No.12

Garlic oil (GAR, Allium sativum L.) has been studied as a feed additive to improve animal production performance and decrease methane emission in ruminants. The present study was designed to determine the possible effect of GAR on fatty acid composition and accumulation in animal fat tissue using a cell model. 3T3-L1 preadipocytes at $2{\times}10^{4}\;mL^{-1}$ were seeded to 24-well plates and allowed to proliferate to reach confluence. The cells were then treated with media containing 0, 2.5, 5, 10, 20 and 40 $\mu{g}$ $mL^{-1}$ of GAR during the differentiation period for 8 days. Media containing dexamethasone, methyl-isobutylxanthine and insulin was applied during the first 2 days of the early differentiation period. On day 8 sub-sets of the wells were stained with oil red-O and the remaining cells were harvested for determination of glycerol-3-phosphate dehydrogenase [EC 1.1.1.8] (GPDH) activity (n = 6) and cellular fatty acid concentration (n = 6). It was found that supplementation of GAR increased (p<0.05) the ratio of monounsaturated fatty acids/saturated fatty acids in the adipocytes and showed inhibitory effect (p<0.05) on the post-confluent proliferation. With relative low dosage, GAR (5-20 $\mu{g}$ $mL^{-1}$) increased (p<0.05) the GPDH activity without affecting the cellular fatty acid concentration, while a high dosage (40 $\mu{g}$ $mL^{-1}$) inhibited (p<0.05) fatty acid accumulation and decreased GPDH activity. Supplementation of GAR had an effect on cell post-confluent proliferation, differentiation and fatty acid accumulation. However, the effect may be diverse and depends on the dose applied.

The Blood Neutrophil-to-lymphocyte Ratio Predicts Survival in Patients with Advanced Hepatocellular Carcinoma Receiving Sorafenib

Zheng, You-Bing,Zhao, Wei,Liu, Bing,Lu, Li-Gong,He, Xu,Huang, Jian-Wen,Li, Yong,Hu, Bao-Shan Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.9

Background and Aim: Increasing evidence correlates the presence of systemic inflammation with poor survival in patients with hepatocellular carcinoma (HCC). The aim of this study was to investigate the prognostic significance of the blood neutrophil-to-lymphocyte ratio (NLR) in patients with advanced HCC who received sorafenib monotherapy. Methods: A total of sixty-five patients with advanced HCC, not eligible for locoregional therapy, treated with sorafenib were enrolled. Potential prognostic factors such as age, gender, tumoral characteristics, performance status and NLR were analyzed. Results: Median OS and TTP for the entire cohort were 10.0 months (95%CI, 7.6-12.3 months) and 4.5 months (95% CI, 4.0-4.9 months). The mean NLR at baseline was 2.89. The median OS of patients with a high NLR (>4) was 6.5 months (95%CI, 5.2-7.7 months) compared with 12.5 months (95%CI, 9.9-15.0) for patients with a normal NLR (${\leq}4$) (P=0.01). Age ${\leq}65$, NLR>4, extrahepatic metastases and vascular invasion were all predictors of poorer overall survival. Multivariate analysis showed that NLR > 4, vascular invasion and extrahepatic metastases were independent predictors of poorer overall survival. The median TTP of patients with a high NLR was 2.5 months (95%CI, 1.4-3.6 months) compared with 4.5 months (95%CI, 3.9-5.1 months) for patients with a normal NLR (P=0.012). Conclusions: High baseline NLR was associated with worse OS and TTP for patients with advanced HCC treated with sorafenib.