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You‑dan Dong,Liang Gao,Feng‑juan Wu,Ren Lin,Yuan Meng,Li‑hong Jia,Xiao‑fei Wang 대한독성 유전단백체 학회 2020 Molecular & cellular toxicology Vol.16 No.2
Background Bisphenol A (BPA) is an environmental estrogen widely exposed to human beings, and there are more studies on its reproductive toxicity, endocrine disruption and neurobehavioral disorders. Recent few studies have found that BPA has immunotoxicity, and its mechanism is not clear. Therefore, the effects of BPA on immune system have attracted extensive attention. The aim of this study was to investigate the effect and mechanism of perinatal exposure to BPA on regulatory T cells (Treg) and Th17 cells in female offspring mice. Methods Twenty-one pregnant C57BL/6 mice were randomly divided into three groups: a control group, low-dose BPA (0.2 μg/mL) and high-dose BPA (2.0 μg/mL) exposure group. All received BPA exposure via drinking water from gestational day 6 to the end of lactation. Female offspring were fed a normal diet and drinking water for 1 month. The percentages of Treg and Th17 cells, the levels of Foxp3 and RORγt protein and IL-17 and TGF-β from spleen tissue or blood were measured in female offspring. Results The percentage of Treg cells and levels of Foxp3 protein decreased, while the percentage of Th17 cells and levels of RORγt protein increased, which showed a dose–effect relationship. The levels of serum TGF-β were significantly lower and the levels of serum IL-17 were statistically higher in BPA-exposed female offspring compared with controls (P < 0.05 or P < 0.01). But there were no statistical difference in the levels of serum TGF-β and IL-17 between 0.2 μg/mL and 2.0 μg/ mL BPA groups (P > 0.05). Conclusion BPA exposure during pregnancy and lactation could cause abnormal differentiation and function of Treg and Th17 cells in female offspring mice, which was associated with down-regulated Foxp3 and up-regulated RORγt protein, respectively. Our findings indicated that BPA exposure during early development may play an important role in the development of autoimmune diseases later.
Hong, You-Kyung,Kim, You-Dan The Korean Society for Aeronautical and Space Scie 2012 International Journal of Aeronautical and Space Sc Vol.13 No.1
In this paper, the Integrated Guidance and Control (IGC) law is proposed for the Rotary Unmanned Aerial Vehicle (RUAV). The objective of the IGC law is to consider the nonlinear dynamic characteristics of the RUAV and to design a guidance law which takes into consideration the nonlinear relationship between kinematics and dynamics. In order to control the RUAV system, sliding mode control scheme is adopted. As the RUAV is an under-actuated system, a slack variable approach is used to generate the available control inputs. Through the Lyapunov stability theorem, the stability of the proposed IGC law is proved. In order to verify the performance of the IGC law, numerical simulations are performed for waypoint tracking missions.
Antibacterial Activity of Curcuma longa against Methicillin-resistant Staphylococcus aureus
You, Yong Ouk,Yu, Hyeon Hee,Jeon, Byung Hun,Jeong, Seung Il,Cha, Jung Dan,Kim, Shin Moo,Kim, Kang Ju 대한동의생리학회,대한동의병리학회 2003 동의생리병리학회지 Vol.17 No.2
Methicillin-resistant Staphylococcus aureus (MRSA) has been emerging worldwide as one of the most important hospital and community pathogens. Therefore, new agents are needed to treat the MRSA. In the present study, we investigated antimicrobial activity of ethyl acetate, methanol, and water extracts of Cureuma longa L. (C. longa) against clinical isolates of MRSA. The ethyl acetate extract of C. longa demonstrated a higher antibacterial activity than the methanol extract or water extract. Since the ethyl acetate extract was more active than other extracts, we examined whether ethyl acetate extract may restore the antibacterial activity of β-lactams and alter the adhesion and invasion of MRSA to human mucosal fibroblasts (HMFs). In the checkerboard test, ethyl acetate extract of C. longa markedly lowered the MICs of ampicillin and oxacillin against MRSA. In the bacterial adhesion and invasion assay, MRSA intracellular invasion were notably decreased in -the presence of 0.125 - 2 mg/ml of C. longa extract compared to the control group. These results suggest that ethyl acetate extract of C. longa may have antibacterial activity and the potential to restore the effectiveness of β-lactams against MRSA, and inhibit the MRSA adhesion and invasion to HMFs.
급성 구강감염부위에서 Staphylococcus lugunensis 의 특성
유용욱,이미성,차정단,김기경,신상희,문상은,김강주 원광대학교 생명공학연구소 1999 생명공학연구소보 Vol.6 No.1
Staphylococcus lugdunensis (S. lugdunensis) is a newly identified pathogenic species of coagulase negative staphylococci and an occasional but not rare cause of severe infections, such as infective endocarditis after dental extraction, bacteremia, osteomyelitis, peritonitis, and soft tissue infections. As antibiotic use increased, resistance rapidly developed. Some strains have plasmids related to antibiotic resistance. To characterize S. lugdunensis in acute oral infection, S. lugdunensis was isolated from the patients with acute oral abscess, osteomyelitis, and normal persons. Antibiotic resistance, in vitro cellular toxicity, in vivo virulence, δ-like hemolysin activity, and synergistic hemolysis on sheep blood agar plates were investigated. The dot blot analysis and Southern blot analysis of staphylococcal DNA was performed with δ-hemolysin gene probe of Staphylococcus aureus. (S. aureus) Staphylococcal DNA was cloned, nucleotide sequence was analysed, and homology was compared with other sequence in Gene Bank. S. aureus, S. lugdunensis, Staphylococcus cohnii (S. cohnii), and other coagulase negative staphylococci (CNS) were isolated from the patients with acute oral infection. The isolation ratio of S. lugdunensis in the patients with infection was higher than that of healthy persons, but the isolation ratio of S. aureus in the patients with infection was similar to that of healthy persons. S. lugdunensis from the patients with acute oral infection showed the resistance to penicillin, methicillin, cephalothin, and clindamycin. S. lugdunensis in the patients had cellular toxicity in vitro and virulence in vivo. All strains of S. lugdunensis had δ-like hemolysin activity against rabbit erythrocytes. Four of the six strains of S. lugdunensis gave synergistic hemolysis with S. aureus on sheep blood agar plates. In dot blot analysis, all strains of S. lugdunensis showed the positive reaction with the probe of δ-hemolysin gene in S. aureus, but a 7.3 kb HindⅢ fragment was observed in the DNA of S. lugdunensis that gave synergistic hemolysis in a Southern blot analysis. The molecular size of partially purified δ-hemolysin was about 50 kd. The cloned fragments from the chromosomal DNA of S. lugdunensis showed the partial homology with the insulin receptor-related and dopamine receptor of humans. These results suggest that S. lugdunensis might be an important pathogen in acute oral infection and show some homology with eukaryotes.
South Korean Study in a Public Health -Preventive Medicine and Sports Environment-
Radut, Dan Silviu,Kim, You-Jin,Min, Byung-Nam,Cho, Ki-Jeoung,Lee, Jong-Young The Korean Society for Preventive Medicine 2009 예방의학회지 Vol.42 No.4
Objectives : The aim of this study was to develop search filters able to retrieve the South Korean scientific output and relating the fields of public health, preventive medicine, and physical education, activity, fitness, exercise and sport in MEDLINE between 2000 and 2007. Methods : The search filters were constructed and applied in MEDLINE accessed through PubMed according to the affiliation and subject. The language and place of publication were identified and the evaluation of the geographical filter performance was done. Results : The search format was successfully elaborated and applied, and the articles originated, published in Korea and/or abroad focusing on the fields of public health, preventive medicine, physical education, activity, fitness, exercise and sport, added to MEDLINE between 2000 and 2007 were retrieved. Publications in six languages originated in South Korea were detected. Conclusions : A geographic search filter determined the South Korean research output, and combined with additional filters focused on specific areas. The dynamics of the scientific output followed an increased evolution in all categories. Articles were written mainly in English/Korean. Further research is recommended on developing search strategies in order to retrieve precise and reliable information.
수확 전 수관 하부 반사필름 처리에 의한 복숭아 과실의 착색 및 관련 유전자의 발현 촉진
이단비(Dan Bi Lee),이국진(Gook Jin Lee),유영준(Young Jun You),안순영(Soon Young Ahn),윤해근(Hae Keun Yun) 한국원예학회 2021 원예과학기술지 Vol.39 No.3
복숭아의 외관은 과실의 품질을 결정하는데 중요한 요인이다. 반사필름에 의한 복숭아 과피의 착색 촉진 효과를 구명하기 위하여, 복숭아(Prunus persica, 품종: Taiko) 과실을 수확 7일전에 과원의 수관 하부에 반사필름을 피복한 후, 수확한 과실의 착색, 가용성 고형물 함량(SSC), 적정산도(TA), 경도(N), 과실의 성숙 및 착색과 관련된 유전자의 발현량을 조사하였다. 반사필름이 처리된 수관 내부의 광투과량(광합성유효방사)이 증가하고, Hunter a값이 현저하게 상승되었다. 반사필름이 처리된 과실의 가용성 고형물 함량, 적정산도 및 경도에는 큰 변화가 없었다. 반사필름을 처리한 과실에서 안토시아닌 생합성과 관련된 유전자(CHS 및 F3H)의 발현은 유도되었으나, 에틸렌 합성에 관여하는 유전자(ACO 및 ERS1) 및 세포벽 연화에 연관된 유전자(PL 및 PME)의 발현은 증가하지 않았다. 이러한 결과는 복숭아 수체 하부의 반사필름 멀칭으로 수관 내부의 광환경을 개선하여 복숭아 과피에서 색소합성 관련 유전자의 발현을 유도하여 착색이 촉진되었음을 의미하는 것이다. Fruit appearance is the most important factor of fruit quality in peach (Prunus persica). In order to determine the promotional effects of increased light in the canopy on peach fruit coloration, reflective film was applied under trees in the field 7 days before the harvest of ‘Taiko’ peach. We then investigated pigmentation of the peel, fruit traits like total soluble solids, titratable acidity, and hardness; the expression of genes related to fruit coloration and ripening in a peach fruits; and photosynthetically active radiation (PAR) inside the tree canopy treated with reflective film mulching. The color of fruit skins treated with reflective film mulching was improved significantly according to their Hunter value compared to untreated fruit. There was no significant change in total soluble solids, acid content, and hardness in fruits treated with reflective film mulching compared to control. Quantitative real-time PCR analysis revealed that transcript levels of anthocyanin biosynthetic related genes (F3H and CHS) were up-regulated; however, the expression of ethylene synthesis-related genes (ACO and ERS1) and ripening-related genes (PL and PME) was not enhanced by reflective film mulching. These results suggest that reflective film mulching improved light irradiance in the tree canopy, which promoted fruit skin coloration via the the induction of anthocyanin biosynthesis in the peel of peach.