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Hiroshi Yoshizumi,Tatsuya Yamada,Kouki Matsuse,Kiyoaki Sasagawa 전력전자학회 1995 ICPE(ISPE)논문집 Vol.1995 No.10
The purpose of this work is to find out performance of speed sensorless direct-field-orientecl controlled induction motor operating in very low speed region using adaptive flux observer. We discuss on the effect of the characteristic of A/D converter to estimated motor speed in this control system by simulation.
Security Technologies, Usage and Guidelines in SCADA System Networks
Mai Kiuchi,Yoshizumi Serizawa 제어로봇시스템학회 2009 제어로봇시스템학회 국제학술대회 논문집 Vol.2009 No.8
The use of off-the-shelf IT systems in SCADA systems has increased, resulting in higher usability at a lower cost. This has introduced a higher risk from cyber threats. As many SCADA systems play an important role in infra-structure and industrial systems, it is important to maintain reliability and security. However, applying cyber security measures should not interfere with the functionality of the SCADA system. We used a model SCADA system to assess various security measures deployed in the system. In addition, standards and guidelines will become more important for selecting appropriate security.
ACA‐specific RNA sequence recognition is acquired via the loop 2 region of MazF mRNA interferase
Park, Jung‐,Ho,Yoshizumi, Satoshi,Yamaguchi, Yoshihiro,Wu, Kuen‐,Phon,Inouye, Masayori Wiley Subscription Services, Inc., A Wiley Company 2013 Proteins Vol.81 No.5
<P><B>Abstract</B></P><P>MazF is an mRNA interferase that cleaves mRNAs at a specific RNA sequence. MazF from <I>E</I>. <I>coli</I> (MazF‐ec) cleaves RNA at A and CA. To date, a large number of MazF homologs that cleave RNA at specific three‐ to seven‐base sequences have been identified from bacteria to archaea. MazF‐ec forms a dimer, in which the interface between the two subunits is known to be the RNA substrate‐binding site. Here, we investigated the role of the two loops in MazF‐ec, which are closely associated with the interface of the MazF‐ec dimer. We examined whether exchanging the loop regions of MazF‐ec with those from other MazF homologs, such as MazF from <I>Myxococcus xanthus</I> (MazF‐mx) and MazF from <I>Mycobacterium tuberculosis</I> (MazF‐mt3), affects RNA cleavage specificity. We found that exchanging loop 2 of MazF‐ec with loop 2 regions from either MazF‐mx or MazF‐mt3 created a new cleavage sequence at (A/U)(A/U)AA and C in addition to the original cleavage site, A and CA, whereas exchanging loop 1 did not alter cleavage specificity. Intriguingly, exchange of loop 2 with 8 or 12 consecutive Gly residues also resulted in a new RNA cleavage site at (A/U)(A/U)AA and C. The present study suggests a method for expanding the RNA cleavage repertoire of mRNA interferases, which is crucial for potential use in the regulation of specific gene expression and for biotechnological applications. Proteins 2013. © 2012 Wiley Periodicals, Inc.</P>