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Chun, Yoon-Keun,Ha, Joo-hun,Hong-Jung-Woo,Oh, Soo-Myung,Kim, Sung-Soo 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1
Yoon-Keun Chun¹,Joohun Ha□Hong-Jung Woo□, Soo Myung Oh□,Sung Soo Kim□ ¹Department of Molecular Biology, College of Medicine,²Department of Surgery, college of Medicine,³Department of Internal Medicine, College of Oriental Medicine,and ⁴East-Weat Medical Reserch Institute,Kyung Hee University, Seoul, Korea. The HBV DNA Amounts in Serum Have No relationship with ALT level and Hetergeneous Population Coexits in Chronic Hepatitis B Virus Infection. Proceedings of International Symposium on East-West Medicine, Seoul. 212-230, 1999. -Hepatitis B is caused by hepadnavirus. Hepatitis B virus replicates through 3.5kb pregenomic RNA intermediate which is regulated by core promoter. Pathogenesis of hepatitis B virus has been bilieved the result of host immune response. But recently many studies have reported that high level of viral replication caused by mutation in core promoter might result in severs hepatitis. But these studies were performed in vitro, not in vivo. So there is yet debate about which factor, viral of host factor, is more important in pathogenesis of hepatitis B virus. So we measured real viral replication level in 204 chronic hepatitis B patients by quantifying HBV DNA from sera by our novel PCR-based more sensitive method, and compared these results with ALT level measured from same sera, which indicates liver cell damage. Surprisingly there are no significant correlation between HBV DNA quantity and ALT level. Then we cloned core promoter region. In SSCP, we found that many viral mutants coexist in one patient. Base on SSCP result, we chose main viral core promoter type in each patients, which is thought to determine overall viral replication level in this patient. Main type of core promoter region of each 41 patients were directly sequenced. And with these we measured promoter activity by luciferase assay system and compared promoter activity with on another. We found tha there were some differences in promoter activity according to core promoter sequences. And we constructed replication-competent viral constructs with core promoter from 41 patients and Transfected these into HepG2 cell and measured HBV DNA by southern blot. There were also differences in HBV DNA quantity according to core promoter sequences. On these all results we investigated correlation between the effect of HBV core promoter on viral replication in vitro and HBN DNA quantity, ALT level from sera of each patients. We found there is no significant correlation among them. As a result, we concluded that in determining severity chronic hepatitis B patients, host factors of each patient is more important rather than replicative activity of virus itself.
Thiol Methyltransferase Activity in Cholestatic Rat Liver Induced by Commom Bile Duct Ligation
곽춘식,--,-- THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 2004 Journal of biomedical laboratory sciences Vol.10 No.1
Changes of thiol methyltransferase (TMT) activity in cholestatic rat liver were studied. Hepatic subcellular and serum TMT activities were determined in cholestatic rat induced by common bile duct (CBD) ligation over a period 28 days. The mitochondrial and microsomal TMT activities in cholestatic rat liver were found to be significantly increased between the 1st and the 28th day after CBD ligation. The TMT activity in serum was significantly increased throughout the experiments. The Vmax values of the above hepatic TMT in cholestatic rat were significantly increased at the 7th day after CBD ligation. However, the Km values of the above hepatic enzymes did not vary in all the experimental groups. Therefore, the results indicate that the biosynthesis of TMT was increased in cholestatic rat liver. The elevated serum TMT activity is most likely caused by increased hepatocytes membrane permeability due to cholestasis mediated liver cell necrosis.
신원철,윤주천,김갑수,최승락,이근억,유주현 江原大學校 産業科學硏究所 1981 産業技術硏究 Vol.1 No.-
In order to use as a starter after drying, optimum growth conditions of Str. thermophilus and L. bulgaricus were investigated. The results were as follows. The growth of Str. thermophilus was reached at stationary phase after 12 hours incubation and L. bulgaricus was after 6 hours. Str. thermophilus was grown well, in the medium containing 10% of skim milk, 2%5 of sucrose, 0.1% of KCl and 0.015%(v./v.) of soy-sauce. In the case of L. bulgaricus, 10% of skim milk, 2% of glucose, 0.5% of bacto-peptone, 0.1% of K₂HPO₄ and 0.02% of pyridoxine was suitable for the maximum growth.
유광성,전현우,박주홍,윤지원,고광삼 朝鮮大學校 附設 醫學硏究所 1986 The Medical Journal of Chosun University Vol.11 No.1
생쥐췌장 베타세포를 보다 순수하게 배양하기 위해 변형 고안한 소배양법을 검정하였다. 이 소배양법에 있어서는 96well plate에 islet cell을 이식하고 decantation한 다음 thimerosal에 노출시켜 많은 viable cell을 얻을 수 있었고, 생후 7일째의 생쥐의 췌장베타세포의 생존율이 가장 높았다. 또 이 소배양법으로 배양한 생쥐췌장 베타세포들은 위상차현미경, FITC-labelled anti-insulin antibody 염색 및 전자현미경으로 검정한 결과 형태학적으로 정상이었다. 기계적 선택방법으로서 생쥐췌장 베타세포의 decantation 최적시간은 세포를 이식한 후 12∼15시간 이었으며, 화학적 선택방법으로 0.6∼0.8㎍/㎖의 thimerosal에 노출시켜 fibroblastoid cell로 부터 생쥐췌장 베타세포의 분리를 증가시킬 수 있었으며, 두가지 방법을 동시에 응용하여 더 좋은 효율을 얻을 수 있었다. 또 이 소배양법에 있어서 cell duplication은 배양후 36시간후에 2배, 72시간후에 3배가 되었으며, 평균 generation시간은 46.8±7.0시간 이었다. 이들 결과로 미루어 보아 이소배양법은 생쥐췌장 베타세포 배양의 유용한 방법으로서, 췌장 베타세포에 연관된 각종 연구에 이용할 수 있을것으로 사료된다.
Taxol합성에 관한 연구. Ⅱ : 3-(2-Hydroxyethyl)-2, 2, 4-trimethyl-3-cyclohexenone SEM ether의 합성
임남수,신동수,정윤성,김정주,박두천,주우홍 昌原大學校 基礎科學硏究所 1995 基礎科學硏究所論文集 Vol.7 No.-
3-(2-Hydroxyethyl)-2,2,4-trimethyl-3-cyclohexenone SEM ether ??, an intermediate in the synthesis of taxol was provided in eight steps. The keto ketal ?? was obtained in three steps from 1,3-cyclohexanedione. Reaction of the potassium enolate of ?? with N-phenyltrifluoromethanesulfonimide provided triflate ?? in 84% yield. Thus palladium-mediated cross-coupling reaction of enol triflate ?? with vinyltributylstannane afforded diene ?? in a 89% yield. 3-(2-Hydroxyethyl)-2,2,4-trimethyl-3-Cyclohexenone SEM ether was prepared from diene ?? by sequential hydroboration [9-BBN, THF, 89%], deprotection[pTsOH ·H₂O, THF-H₂O,~100%], and silyation [SEMC], iPr₂NEt, nBu₄NI, CH₂Cl₂, 94%]
Yoon, So Yoon,Ryu, Yon Ju,Kang, Hye Won,Baek, Doo Hyun,Kim, Soo Hyun,Choi, Min Young,Park, Hye Sung,Lee, Jin Hwa,Chun, Eun Mi,Chang, Jung Hyun,Cho, Young Joo,Jun, Yoon Hee,Shim, Sung Shine,Lee, Mi Ae 이화여자대학교 의과학연구소 2010 EMJ (Ewha medical journal) Vol.33 No.2
연구배경:2009년 4월 새로운 인플루엔자 A(H1N1) 바이러스에 의한 감염은 전세계적으로 대유행이 시작되었다. 본 연구에서는 대한민국의 한 대학병원에서 발생한 H1N1 인플루엔자 감염환자 중에서 청소년과 성인 입원 환자를 대상으로 임상적 특성과 예후에 대하여 2009년 9월부터 2010년 1월까지 조사하여 알아보고자 하였다. 방 법:대유행 H1N1 인플루엔자로 확진된 13세 이상의 환자의 의무기록을 후향적으로 분석하였으며 대유행 H1N1 인플루엔자는 real-time reverse transcriptase-polymerase chain reaction 시행하여 확진하였다. 결 과:43명의 대유행 인플루엔자 입원 환자 중에서 7명(16%)은 중환자실 치료를 받았으며 이중 1명(2%)은 사망하였다. 평균 연령은 45세였고 기저질환으로 천식, 당뇨, 허혈성 심장질환, 기관지 확장증, 암의 순서로 많은 빈도수를 보였다. 임상증상으로 발열이 가장 주된 증상이었으며 그 외에 기침, 호흡곤란, 콧물, 근육통 또는 관절통, 인후통을 호소하였다. 입원 시 42명 중 25명의 환자에서 흉부 방사선 검사 결과 폐렴을 동반하였고 만성호흡기 질환의 악화를 보인 경우가 11명의 환자에서 관찰 되었다. 또한 23명의 Fish환자에서 산소공급이 필요하였으며 이중 2명은 급성 호흡부전으로 인하여 기계 환기가 필요하였다. 중환자실 입원을 요한 7명의 환자들은 모두 기저질환을 가지고 있었으며 이중 5명은 천식, 3명은 허혈성 심장 질환이 있었고 임신 상태와 당뇨가 각각 1명으로 나타났다. 확진된 모든 환자들은 증상 발현으로부터 평균 2일 내에 항바이러스제를 처방 받았다. 결 론:천식은 입원치료를 요하는 대유행 인플루엔자 감염환자에서 가장 흔하게 동반된 내과적 질환이었으며 특히 허혈성 심장질환 동반하는 환자의 경우 중환자실 입원을 필요로 함을 알 수 있으며 연구 결과 대부분의 환자에서 항바이러스제 치료에 효과적인 것으로 나타났다.
Antiviral Activity of Ascorbic Acid Against Herpes Simplex Virus
Yoon, Joo-Chun,Cho, Jeong-Je,Yoo, Seung-Min,Ha, Youn-Mun The Korea Society for Microbiology 2000 大韓微生物學會誌 Vol.35 No.1
In order to explore the potential of ascorbic acid supplementation for the prevention and treatment of herpes simplex viral diseases, plaque reduction assays were performed. Ascorbic acid as well as copper chloride/ferric chloride were added to wells containing Vero cells infected with herpes simplex virus type 1 (HSV-1), and the infectivity of HSV-1 was determined. Since copper and iron are major transition metals in human plasma, near the normal human plasma concentrations of them were used for experiments. When Cu(II) and Fe(III) were applied, there were no significant differences between virus control and Cu(II)/Fe(III)-treated groups. But, when appropriate concentrations of ascorbic acid were added to wells, meaningful differences between control and ascorbate-treated groups were found. In the presence of Cu(II)/Fe(III) at $5.8/3.7\;{\mu}M$, 72-h treatment with ascorbate at $50\;{\mu}M$ reduced HSV-1 infections to $10.77%{\pm}4.25%$ (P < 0.001) and $500\;{\mu}M$ did to $3.06%{\pm}1.62%$ (P < 0.001). Moreover, the cytotoxicities for Vero cells at those concentrations were insignificant (P > 0.05). Current recommended dietary allowance (RDA) of ascorbic acid is 60 mg/day, and the oral intake of 60 mg/day of ascorbic acid yields plasma ascorbic acid at 45 to $58\;{\mu}M$ in a healthy adult man. Therefore, the results of this study suggest that the maintenance of appropriate level (more than $50\;{\mu}M$) of ascorbic acid in human plasma by appropriate amount (more than the RDA) of ascorbic acid supplementation may be helpful for the prevention and treatment of diseases caused by HSV -1 in an adult man. In addition, this study also suggests that ascorbic acid may be useful for the prophylaxis of fatal HSV-1 infections in neonates and the prevention of HSV-1 reactivation in immunocompromised hosts.