Apoptosis and necroptosis‑inducing effects of arctigenin on nasal septum carcinoma RPMI‑2650 cells in 2D and 3D culture

Yoon‑Jin Lee,Kwan‑Sik Park,Byoung Joon Baek,Kyong‑Ae Lee,Sang‑Han Lee 대한독성 유전단백체 학회 2020 Molecular & cellular toxicology Vol.16 No.1

Backgrounds Arctigenin derived from the seeds of Arctium lappa Linnaeus is known as an anticancer drug candidate by targeting various pathways involved in anticancer therapy. Methods Using 2D monolayer and 3D spheroid culture systems in nasal septum carcinoma RPMI-2650 cells, the effects of arctigenin and dexamethasone on cell viability, ROS levels, ATP level, mitochondrial function, apoptosis and necroptosis were examined. Results The combination treatment of both compounds induced strong cytotoxicity, accompanied by increases of sub-G0/ G1 peak, annexin V-PE-positive cells, and ROS levels, loss of mitochondrial membrane potential, and decrease of cellular ATP content. These changes were observed as simultaneous induction of DNA damage, apoptosis, and necroptosis. A series of changes by arctigenin and dexamethasone were efficiently restored by decreasing ROS levels or supplementing ATP. Treatment of 3D spheroids with arctigenin and dexamethasone decreased cell viability in the spheroids, but it was slightly resistant than cells under 2D conditions. In addition, this phenomenon was accompanied by an increase in mediators for both apoptosis and necroptosis. Conclusion Results of this study suggest that the apoptosis and necroptosis-inducing effects of arctigenin are associated with ATP depletion due to oxidative mitochondrial dysfunction.

인터넷 우체국 시스템 구현

尹賢植,郭允植 충주대 2000 한국교통대학교 논문집 Vol.35 No.2

There have been dramatic technological changes in the telecommunication environment over the past decade in the world.These changes include the conversion to all-digital, software defined networks.In this paper, we implemented the internet post office system.

Three-Dimensional Z-Plasty(Yoon's Method)를 이용한 내안각 췌피 성형술

박흥식,김우신,윤진호,나민화,김한중 대한미용성형외과학회 1997 Archives of Aesthetic Plastic Surgery Vol.3 No.1

The presence of epicanthal folds in Asian eyelids is one of their unique features, in combination with the lack of supratarsal folds. Western culture has influenced many people to prefer to eliminate the prominent epicanthal fold. A number of surgical techniques have been suggested for their correction. However, difficulty with design, excessive and prominent scarring of medial canthal and nasal area, recurrence, and rigidity of application are potential problems associated with many procedures. This paper describes a epicanthoplasty with three-dimensional Z-plasty. Through epicanthal fold incision, the medial canthal tendon is medially advanced and sutured to the periosteum of nasal bone. After the transposition of the flaps, trimming of the flap is usually required. Three-dimensional Z-plasty creates the attractive eyes. From March, 1995 to March, 1997, the technique was applied to 37 patients with epicanthal fold and was performed with or without double-fold operation. There were 34 females and 3 males with ages ranging from 17 to 30 years. Through 2 years follow-up, this technique has delivered esthetically good results with minimal postoperative scar and could made the reduction of ICD from 40 ± 2.13 mm to 34 ± 1.98 mm. The advantages of epicanthoplasty procedure using three-dimensional Z-plasty(Yoon's method) are as follows; 1) simple in design 2)minimal postoperative scar in the medial canthal area 3) versatile in its application 4) no recurrence 5) no hypertrophic scar 6) preserving ethnic identity.

전염소 투입 및 정수지 구조가 정수장 총소독능에서 갖는 중요성 평가

윤제용(Je Yong Yoon),변석종(Seok Jong Byun),최유식(Yu Sik Choi) 한국물환경학회 2001 한국물환경학회지 Vol.17 No.3

Disinfection is one of the most important processes in water treatment plants since it protects consumers from waterborne diseases. In Korea, chlorine which is most frequently used as a disinfectant is generally applied in two ways: prechlorination and postchlorination. Postchlorination is to supply chlorine to the influent of a clearwell. While, prechlorination is to add chlorine to rapid mixing chamber or water intake in plants. Major disinfection reaction occurs in the step of post chlorination. Therefore, frequently, prechlorination is not counted on in evaluating disinfection credit. This study was conducted to evaluate the importance of prechlorination and the existence of baffling wall in clearwell with the United States disinfection requirement(Surface Water Treatment Rule). Eight water plants were considered. Yoon`s classification(1997) was selected in classifying the prechlorination practice in water treatment plant. It was found that most of the prechlorination practices performed in water plants(three out of four types) were not important in gaining any significant disinfection credit. One prechlorination practice conducted to remove ammonia in raw water became significant in meeting the disinfection requirement. Despite the existence of baffling walls of clearwell, T_(10)/T varied considerably in the range of 0.3-0.6, indicating that the existence of baffling wall itself does not guarantee the goodness of plug flow characteristics. For some plants, T_(10)/T were lower than that of US-EPA conservative recommendation which is considered when tracer test could not be performed.

야생 팽나무버섯의 생리활성 분석

윤향식 외 한국버섯학회 2010 한국버섯학회지 Vol.8 No.1

충북지역에서 수집한 야생 팽나무버섯의 식품소재로의 가능성을 조사하기 위해 동결 건조한 후 일반성분 및 전자공여능, 총폴리페놀 함량, ACE 저해도와 α -glucosidase 저해도 등 생리활성을 분석하였다. 대조군으로 갈뫼팽이버섯과 4종의 수집 야생 팽나무버섯의 일반성분은 건물중으로 분석하였으며, 조단백질은 17.1~21.3 g/100g, 조지방은 4.1~4.9 g/100g, 탄수화물은 65.9~70.5 g/100g, 조섬유는 7.9~18.1 g/100g, 조회분은 5.7~8.4g/100g 범위를 나타내었다. 전자공여능은 63.35~69.31%로 시료간 큰 차이가 없는 것으로 나타났으며, 총폴리페놀 함량은 9.83에서 11.14 mg/g로 대조구와 유사하였다. ACE 저해도는 대조구인 갈뫼 팽이버섯과 수집종인 CBMFV-02가 각각72.91%, 71.44%로 높은 값을 나타내었으며, α -glucosidase 저해도는 CBMFV-01이 46.55%로 가장 높은 값을 나타내었다. In this study, for the development of useful food materials, the proximate composition(total carbohydrates, crude fiber, crude protein, fat and ash) and biological activities(electron donating activity, total polyphenol contents, ACE inhibitory and □-glucosidase inhibitory effects) were determined in the Garlmoe and the four wild Flammulina velutipes. As a results, the dry matter of mushrooms contained large amounts of carbohydrates, from 65.9 to 70.5 g/100g dry weight. the wild collected Flammulina velutipes proved to be an especially good source of crude fiber(7.9~18.1 g/100 g dry weight). Crude protein, fat, and ash contents of the mushrooms ranged 17.1~21.3, 4.1~5.2, and 5.7~8.4 g/100g dry weight, respectively. The electron donating activity ranged from 63.35 to 69.31% and total polyphenol contents ranged from 9.83 to 11.14 mg/g in these mushrooms. ACE inhibitions were high in Garlmoe and CBMFV-02 strain. □-glucosidase inhibition was the highest in CBMFV-01(46.56%) strain and lowest in Garlmoe(15.91%).

관상동맥 스텐트 내 재협착의 발생에 미치는 임상적 관동맥 조영술적 요인들

윤혁준,김기식,박형섭,이영수,현대우,한성욱,허승호,김윤년,김권배,박남희,원경숙 대한순환기학회 2003 Korean Circulation Journal Vol.33 No.12

12주간의 유산소성 운동이 비만 초등학생의 신체조성과 혈액성분에 미치는 영향

윤미수,최건식,고성경,정소봉 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.5

[Purpose] The purpose of this study was to investigate the effect of aerobic exercise training on the body composition and blood components in obese elementary children after 12 weeks. [Methods] Subjects of this study were composed of 20 male students ranging in age from 9 to 12 years who were classified into two groups, obese exercise group(n=10) and normal control group(n=10). For 12 weeks, obese exercise group performed the aerobic exercise training that the program was composed by Yoon. The blood samples were taken before and after 12 week Paired t-Test was adopted to analyse the difference between before and after aerobic exercise training. [Results] body fat of obese exercise group was decreased 4.8% after aerobic exercise training(p<.01) and that of normal control group was decreased 0.1% during same time(NS). Among the blood lipoprotein, total cholesterol(p<.01), LCL-C(p<.01), and triglycerides(p<.05) of obese exercise group were decreased after aerobic exercise training, but not changed HDL-C of same group. Red blood cell, Hemoglobin(p<.65) of obese exercise group were increased after aerobic exercise training. Glucose of obese exercise group were decreased after aerobic exercise training(p<.05), but not changed in Ca and P. [Conclusion] In conclusion, aerobic exercise training for 12 weeks seems to reduce body might and body fat, and to change the profiles of blood Component in obese elementary children.

임신 40주와 출산 후 6주에 있는 20대 임산부 보행의 역학적 특성 비교

윤희중,문동안,정남주,금명숙,이경식,유실,김영란,한윤수 한국운동역학회 2000 한국운동역학회지 Vol.10 No.1

본 연구는 임신 40주와 출산후 6주간에 있는 임산부의 보행 동작의 특정을 규명함으로써 이와 관련된 연구자들에게 기초자료를 제공하고자 실시하였다. 이를 위해 임신 4주에 있는 임산부를 대상으로 1차 실험을 실시하였으며 동일한 대상자를 출산후 6주에 2차로 실험하여 자료를 수집하였다. 디지타이징 시 오차를 최소화하고 인체관절점의 분별을 위해 21개의 반사마커를 부착하였다. 10m의 보행주로를 걷는 동안 두 대의 비디오카메라를 이용하여60Hz로 자료를 수집하였으며 동시에 100Hz 지면반력자료를 수집하였다.수집되어진 자료는 DLT 방법을 사용하여 분석하였으며 이의 분석에 Kwon3d 프로그램을 활용하였다. 분석결과 다음과 같은 결론을 얻었다. 1. 임신 40주와 출산 후 6주의 보행 시 지지시간과 스윙시간의 비율이 다르게 나타났으며 보폭과 보간, 활보장은 임신 40주에서 더 큰 값을 보였다. 상체 전경각 역시 임신 40주에서 더 큰 값을 보였다. 힙관절, 무릎관절, 발목관절각도는 S1이 출산후 6주에서 큰 값을 보인반면 S2는 출산전후에 큰 변화를 보이지 않다. 2. 상하지면반력의 경우 FP1과 FP2는 임신 40주보다는 출산 6주에서 큰값을 보였지만 FP3는 임신 40주에서 보다 큰값을 보이고 있었다. 전후지면반력의 경우 출산전과 후 모두 Fy2에서 보다 큰 값을 보이고 있었다. This Study was to investigate kinematical characteristic comparison of the gait pregnant 40weeks and after delivery 6weeks 20´s women and provide the basic data. To accomplish this study, in the first, carry out an experiment with the pregnant women in the pregnant 40weeks on the videotape, and than the second, make an experiment with the kinematic data. Kinematic data were filtered with a low-pass, fourth-order Butterworth filter with an effective cut off frequency of 6Hz. The conclusions were drawn as follows; 1. Floor contact Periods(stance and swing rate) were differed from before and after the delivery and a pregnant 40 weeks was greater than after delivery 6 weeks in the step, width, stride. Also, a pregnant 40 weeks was greater than after delivery 6 weeks in the front angular. And the other angular components were represented similar pattern. 2. In the vertical load, delivery 6 weeks was grater than a pregnant 40 weeks in the FP1(first peak) and FP2(valley). And FP3(second peak)was represent that a pregnant 40 weeks was greater than delivery 6 weeks. Also, in the horizontal shear, Fy2(progressional force)was greater than Fy1(breaking force) about time of the delivery.

카올린으로부터 질화알루미늄 분말 합성

이윤복,서기원,류봉기,김광호,박희찬,장윤식 釜山大學校生産技術硏究所 1996 生産技術硏究所論文集 Vol.50 No.-

카올린으로 부터 수화염기성황산알루미늄을 제조하고, 이것을 탄소열환원질화하여 질화알미늄 분말을 합성하였다. 수화염기성황산알루미늄은 카올린으로 부터 제조된 수화황산알루미나 0.5M 및 요소 5M인 용액으로 부터 얻은 것이 가장 미세하였다. 탄소열환원질화법으로 질화알미늄을 합성할 때 활성화탄과 염기성황산알루미늄의 질량비가 2인 조성이 가장 적합하였으며, 이 때 얻어진 질화알미늄은 0.3-1㎛의 submicron급 분말이었다. Aluminum nitride powder was synthesized by carbothermal reduction-nirtridation using kaolin- derived hydrated basic aluminum sulfate. The hydrated basic aluminum sulfate powder produced from the solution of 0.5M hydrated aluminum sulfate and 5M urea showed very fine particles. The aluminum nitride showed the submicron powder of 0.3-1㎛ in the optimum condition with 2.0-weight ratio of activated charcol to hydrated basic aluminum sulfate.

함수 언어에 기반을 둔 구조적 분석의 정형적 명세와 실행

신윤식,김태남,임은기,이기수 金烏工科大學校 1993 論文集 Vol.14 No.-

An executable specification language for Structured Analysis specification and its execution model are presented. The model is based on Applicative State Transition system and the interpretation of data flow diagram in a viewpoint of data availability. AST system receives at most one input and does not accept until an output is issued. Our model is extended with availability list so that concurrency inherent in DFD can be treated.