The HBV DNA Amounts in Serum Have No relationship with ALT level and Hetergeneous Population Coexits in Chronic Hepatitis B Virus Infection

Chun, Yoon-Keun,Ha, Joo-hun,Hong-Jung-Woo,Oh, Soo-Myung,Kim, Sung-Soo 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Yoon-Keun Chun¹,Joohun Ha□Hong-Jung Woo□, Soo Myung Oh□,Sung Soo Kim□ ¹Department of Molecular Biology, College of Medicine,²Department of Surgery, college of Medicine,³Department of Internal Medicine, College of Oriental Medicine,and ⁴East-Weat Medical Reserch Institute,Kyung Hee University, Seoul, Korea. The HBV DNA Amounts in Serum Have No relationship with ALT level and Hetergeneous Population Coexits in Chronic Hepatitis B Virus Infection. Proceedings of International Symposium on East-West Medicine, Seoul. 212-230, 1999. -Hepatitis B is caused by hepadnavirus. Hepatitis B virus replicates through 3.5kb pregenomic RNA intermediate which is regulated by core promoter. Pathogenesis of hepatitis B virus has been bilieved the result of host immune response. But recently many studies have reported that high level of viral replication caused by mutation in core promoter might result in severs hepatitis. But these studies were performed in vitro, not in vivo. So there is yet debate about which factor, viral of host factor, is more important in pathogenesis of hepatitis B virus. So we measured real viral replication level in 204 chronic hepatitis B patients by quantifying HBV DNA from sera by our novel PCR-based more sensitive method, and compared these results with ALT level measured from same sera, which indicates liver cell damage. Surprisingly there are no significant correlation between HBV DNA quantity and ALT level. Then we cloned core promoter region. In SSCP, we found that many viral mutants coexist in one patient. Base on SSCP result, we chose main viral core promoter type in each patients, which is thought to determine overall viral replication level in this patient. Main type of core promoter region of each 41 patients were directly sequenced. And with these we measured promoter activity by luciferase assay system and compared promoter activity with on another. We found tha there were some differences in promoter activity according to core promoter sequences. And we constructed replication-competent viral constructs with core promoter from 41 patients and Transfected these into HepG2 cell and measured HBV DNA by southern blot. There were also differences in HBV DNA quantity according to core promoter sequences. On these all results we investigated correlation between the effect of HBV core promoter on viral replication in vitro and HBN DNA quantity, ALT level from sera of each patients. We found there is no significant correlation among them. As a result, we concluded that in determining severity chronic hepatitis B patients, host factors of each patient is more important rather than replicative activity of virus itself.

검단산(경기도 하남시)의 거미상

김주필(Joo Pil Kim),최한석(Han Seak Choi),이형민(Hyung Min Lee),이형진(Hyung Jeen Lee),이태후(Tae Hoo Lee),심주영(Ju Young Sim),조윤규(Yoon Kyu Jo),조원규(Won Kyu Jo),이준이(Joon Lee Lee) 한국거미연구소 2018 한국거미 Vol.34 No.2

2018년 10월 2일부터 3일까지 경기도 하남시 소재 검단산을 주야로 한국거미연구회 회원 13명(김주필, 최한석, 이형민, 이형진, 심주영, 이태후, 조윤규, 조원규, 이준이, 이현동, 윤혜원, 이광섭, 김형주)이 채집한 결과 22과 43속 56종이 채집되어 이에 보고 발표하는 바이다. From October 2 to 3 in 2018. Koran Arachnological Study members investigated the Spiders in the whole area Gyeonggi-do Hanam-si, and reported 22 Families, 43 Genus 56 Species of spiders. The collected spiders are placed (stored) in the Joo-Pil Spider museum.

급성전골수성백혈병에서 백혈구성분채집술에 이은 치명적인 뇌출혈 2예

오윤정,박소윤,김윤정,한동석,김현수,최진혁,남동기,임호영,김효철,주희재 아주대학교 의과학연구소 1998 아주의학 Vol.3 No.1

The aim of this study is to report 2 cases of acute promyelocytic leukemia who died from intracranial hemorrhage following leukapheresis and to provide proper preventive measures against hemorrhage following leukapheresis. From 1994 to 1997, a total of twenty-six patients with leukemia underwent leukapheresis to control hyperleukocytosis at Ajou University Hospital. Two patients with acute promyelocytic leukemia received all-trans retinoic acid but developed drug-induced hyperleukocytosis. Shortly after leukapheresis, they died from intracranial hemorrhage. The risk factors for fatal hemorrhage are thought to be coexisting disseminated intravascular coagulation(DIC), thrombocytopenia aggravated by leukapheresis, exacerbated coagulopathy related to mechanical trauma through leukapheresis and the excess use of citrate during leukapheresis. To reduce the risk of the bleeding associated with leukapheresis, it is necessary to replace platelet sufficiently before and after leukapheresis and to give calcium to correct coagulopathy induced by excess citrate which is used as anticoagulant as well as the correction of disseminated intravascular coagulation.

독도(경상북도 울릉군)의 거미상

김주필(Joo Pil Kim),최한석(Han Seak Choi),지승환(Seung Hwan Ji),김대희(Dae Hee Kim),변진관(Jean Koan Byun),이형민(Hyung Min Lee),조형진(Hyung Jeen Jo),이형진(Hyung Jeen Lee),이태후(Tae Hoo Lee),한정혁(Jung Huk Han),조윤규(Yoon Kyu Jo 한국거미연구소 2018 한국거미 Vol.34 No.2

2018년 7월 27일부터 독도 지역을 한국거미연구회 회원 17명(김주필, 최한석, 김대희, 지승환, 이형민, 변진관, 조형진, 이형진, 이태후, 한정혁, 조윤규, 조원규, 이현동, 이윤정, 조용하, 윤혜원, 이용화)이 채집한 결과 7과 9속 12종이 채집되어 이에 보고 발표하는 바이다. 2018 July 27th, Koran Arachnological Study members investigated the Spiders in the whole area Dok Island (Gyeongsangbuk-do, Ulleung-gun), and reported 7 Families, 9 Genus 12 Species of spiders. The collected spiders are placed (stored) in the Joo-Pil Spider museum.

울릉도(경상북도 울릉군)의 거미상

김주필(Joo Pil Kim),최한석(Han Seak Choi),김대희(Dae Hee Kim),지승환(Seung Hwan Ji),이형민(Hyung Min Lee),변진관(Jean Koan Byun),이형진(Hyung Jeen Lee),조형진(Hyung Jeen Jo),한정혁(Jung Huk Han),이태후(Tae Hoo Lee),조윤규(Yoon Kyu Jo 한국거미연구소 2018 한국거미 Vol.34 No.2

2018년 7월 26일부터 28일까지 울릉도 나리분지, 독도 지역을 주야로 한국거미연구회 회원 17명(김주필, 최한석, 김대희, 지승환, 이형민, 변진관, 조형진, 이형진, 이태후, 한정혁, 조윤규, 조원규, 이현동, 이윤정, 조용하, 윤혜원, 이용화)이 채집한 결과 20과 43속 60종이 채집되어 이에 보고 발표하는 바이다. From July 26 to 28 in 2018. Koran Arachnological Study members investigated the Spiders in the Ulleung Island (Gyeongsangbuk-do, Ulleung-gun), and reported 20 Families, 43 Genus 60 Species of spiders. The collected spiders are placed (stored) in the Joo-Pil Spider museum.

화야산(경기도 가평군)의 거미상

김주필(Joo Pil Kim),최한석(Han Seak Choi),이형민(Hyung Min Lee),이형진(Hyung Jeen Lee),이태후(Tae Hoo Lee),심주영(Ju Young Sim),조윤규(Yoon Kyu Jo),조원규(Won Kyu Jo),이준이(Joon Lee Lee) 한국거미연구소 2018 한국거미 Vol.34 No.2

2018년 10월 13일부터 14일까지 경기도 가평군 소재 화야산을 주야로 한국거미연구회 회원 13명(김주필, 최한석, 이형민, 이형진, 심주영, 이태후, 조윤규, 조원규, 이준이, 이현동, 윤혜원, 이광섭, 김형주)이 채집한 결과 18과 41속 48종이 채집되어 이에 보고 발표하는 바이다. From October 13 to 14 in 2018. Koran Arachnological Study members investigated the Spiders in the whole area Gyeonggi-do Gapyeong-gun, and reported 18 Families 41 Genus 48 Species of spiders. The collected spiders are placed (stored) in the Joo-Pil Spider museum.

출아효모에서 TAR 클론닝법을 이용한 고등동물의 게놈으로부터 특정 염색체 부위의 분리

박정은,윤영호,이윤주,김광섭,윤세련,안태진,임선희,선우양일 동아대학교 기초과학연구소 2003 基礎科學硏究論文集 Vol.20 No.1

복잡한 게놈 분석에 용이하도록 효모의 인공 염색체(YAC) 클론닝 시스템은 발달되어왔다. YAC은 박테리아의 인공 염색체(BAC)보다 더 큰 단편을 클론닝할 수 있고, 또한 클론된 단편을 쉽게 변형시킬 수 있다. 형질전환과 연계된 재조합법(Transformation-Associated Recombination; TAR)은 게놈 라이브러리를 만들지 않고 직접 게놈 DNA로부터 분리하고자 하는 유전자나 특정 염색체 부분을 클론닝 할 수 있는 방법이다. 이 방법은 spheroplast transformation을 수행하는 동안, 목적으로 하는 유전자의 5' 그리고 3' 염기 배열(hooks)을 지닌 TAR 벡터와 게놈 DNA 사이에서 일어나는 상동성 재조합에 의해 이루어진다. 효모 내의 in vivo 재조합을 이용한 TAR 클론닝법은 복잡한 게놈으로부터 목적의 염색체 부분을 원형 YAC의 형태로서 선택적으로 분리할 수 있다. 그러므로 TAR 클론닝 법은 특정 염색체로부터 YACs을 만드는데 매우 유용하여, 전체 게놈으로부터 특이적 유전자나 유전자의 family를 분리하는데 효과적인 방법으로 사료된다. Yeast artificial chromosome (YAC) cloning systems have advanced the analysis of complex genomes considerably. They permit the cloning of larger fragments than do bacterial artificial chromosome systems, and the cloned material is more easily modified. Transformation-associated recombination (TAR) is a cloning technique that allows specific chromosomal regions or genes to be isolated directly from genomic DNA without prior construction of a genomic library. This technique involves homologous recombination during spheroplast transformation between genomic DNA and a TAR vector that has 5' and 3' gene targeting sequences (hooks). Using in vivo recombination in yeast, TAR cloning selectively isolates, as circular YACs, desired chromosome segments or entire genes from complex genomes. We propose that TAR cloning can provide an efficient means for generating YACs from specific chromosomes and that TAR cloning may be useful for isolating families of genes and specific genes from total genome DNA.

화경버섯에서 얻은 펩스타틴-불감응성 카르복시프로테이나아제의 기질 특이성

윤주억 동국대학교 산업기술연구원 1996 산업기술논문집 Vol.8 No.-

화경버섯(Lampteromyces japonicus)에서 처음으로 카르복시프로테이나아제(LCP)를 분리하였으며, 이 효소는 아스파르트산 프로테이나아제의 억제제인 펩스타틴과 diazoacetyl-DL-norleucine methylester에 대하여 감수성을 나타내지 않았다. LCP의 기질 특이성은 P5-P4-P3-P2-Phe-Nph-P2'-P3'(P5, P4, P3, P2, P2', P3'은 여러가지 아미노산 잔기를, Nph는 p-nitro-L-phenylalanine을 나타내며, 효소의 분해자라는 Phe-Nph 사이의 결합이다)와 같은 구조로 된 발색성 합성 펩티드 기질로서 연구하였다. 합성된 28가지 기질중, Lys-Val-Leu-Leu-Phe-Nph-Arg-Ala가 가장 최적 기질임이 밝혀졌다. 이 펩티드 기질에 대한 LCP의 효소반응 속도 상수들은 각각 K_m=15.5 μM, k_cat=12.4 s^-1, k_cat/K_m=0.80 μM^-1ㆍs^-1이었다. Tyrostatin은 LCP에 대하여 경쟁적 억제제 (Ki=2.1 nM)임이 밝혀졌다. Lampteromyces carboxyl proteinase (LCP), isolated from Lampteromyces japonicus, was the first example of a unique carboxyl proteinase which was obtained from mushrooms and was insensitive to aspartic proteinase inhibitors, such as pepstatin and diazoacetyl-DL-norleucine methylester. The substrate specificities of LCP was studied using a series of synthetic chromogenic peptide substrates with the general structure, P5-P4-P3-P2-Phe-Nph-P2'-P3'(P5, P4, P3, P2, P2', and P3' were a variety of amino acids, Nph was p-nitro-L-phenylalanine, and the Phe-Nph bond was cleaved). LCP was shown to hydrolyze a synthetic substrate, Lys-Val-Leu-Leu-Phe-Nph-Arg-Ala, most effectively among 28 substrates. The kinetic parameters of this peptide for LCP was K_m=15.5 μM, k_cat=12.4 s^-1, and k_cat / K_m=0.80 μM^-1ㆍs^-1ㆍ Tyrostatin was found to be a compeptitive inhibitor for LCP with a Ki value of 2.1 nM.

합성 Peptide Benzyloxycarbonyl-glycyl-L-tyrosyl-L-phenylalanyl-glycine 및 그 Ethyl Ester에 대한 pepsin작용

윤주억 동국대학교 농림과학연구소 1969 農林科學 論文集 Vol.3 No.-

The synthesis is described of new pepsin substrates of benzyloxycarbonyl-glycyl-L-tyrosyl-L-phenylalanyl-glycine and benzyloxycarbonyl-glycyl-L-tyrosyl-L-phenylalanyl-glycine ethyl ester for studies on the specificity of pepsin, and thin layer chromatographic examination of the peptides prepared showed that the new substrates are homogeneous. And also, same examination of the incubation mixtures showed that two synthetic substrates are cleaved by pepsin at the L-tyrosyl-L-phenylalanyl bond and hydrolysis of these substrates by pepsin is achieved without transpeptidation. It is found that synthetic peptides are moderately soluble with the amount of the substrate up to a concentration of 0.7 mM in aqueous sodium citrate buffers(0.04 M) in the pH range 1.8-4.0, thus obviating the necessity for the adding of an organic solvent in the assay mixture. The kinetic parameters for synthetic substrates are tabulated in the following table. The data in the table indicate that the susceptibility of synthetic peptides to peptic hydrolysis ◁표 삽입▷(원문을 참조하세요) are relatively large and the change of the carboxyl terminal group of synthetic substrate from glycine ethyl ester to glycine causes a small decrease in the susceptibility of the L-tyrosyl-L-phenylalanyl bond.

부산신항 남컨테이너부두 배후지 토지이용계획

윤철현,김주석,강지은 東亞大學校 建設技術硏究所 2006 硏究論文集 Vol.29 No.2

해양 형 국가에 있어 국토의 개방적 이용에는 항만개발이 요체가 된다. 이 때문에 정부는 부산 가덕도를 중심으로 경제자유구역 및 남, 북 컨테이너부두 개발에 박차를 가 하고 있다. 부산과 진해에 이르는 광대한 경제자유구역에는 기술의 집중과 창조는 물론 대 규모의 사람과 물류의 흐름이 예상된다. 신항 만 은 이들 흐름을 수용할 수 있는 종합항만 으로 개발되어야 한다. 본 연구는 신항만의 남 「컨」 부두 배후지 개발은 종합항만의 성격에 부합하는 기능을 유인하는데 일차적 목적을 두고 있고, 이를 달성키 위한 합리적 토지이용계획을 수립 하는데 이차적 목적이 있다.