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장내의 탄수화물과 지방 흡수 억제를 통한 체지방 및 비만 개선 효과에 관한 연구
정은희,윤승원,이홍석,윤유식,유경미,황인경 한국조리과학회 2003 한국식품조리과학회지 Vol.19 No.1
In a previous study, a dietary supplement was developed in our lab using natural herbal extracts against digest enzyme activity in GI tract for weight control. This natural herbal extracts could regulate absorption of glucose and lipid by the inhibition of digest enzyme activity. In this study, we screened the natural herbs that inhibit glucoamylase activity and developed an water extract of cinnamon. The cinnamon extract delayed and decreased the increment of carbohydrate degradation through the inhibition of glucoamylase activity in vitro. Fifty volunteers were subjected to the intake of the herbal extracts by taking twice a day for 60 days. As a result, the treated subjects lost 3kg of body weight and 3.5kg of body fat mass after the treatment. Furthermore, the body mass index and waist size were significantly decreased during the experimental period. Above results suggested that the administration of the dietary additives composed of cinnamon and natural herbal extract improves the obesity by the decrement of body weight and body fat mass.
The Smurf ubiquitin ligases regulate tissue separation via antagonistic interactions with ephrinB1
Hwang, Yoo-Seok,Lee, Hyun-Shik,Kamata, Teddy,Mood, Kathleen,Cho, Hee Jun,Winterbottom, Emily,Ji, Yon Ju,Singh, Arvinder,Daar, Ira O. Cold Spring Harbor Laboratory Press 2013 Genes & development Vol.27 No.5
<P>Smad ubiquitin regulatory factors (Smurfs) play important roles in cell growth and differentiation. Hwang et al. present a new mechanism of Smurf regulation in <I>Xenopus</I> with evidence that Smurf1 and Smurf2 essentially compete for association with ephrin B1 (Smurf2 triggers ephrinB1 degradation, and Smurf1 counteracts it) and so regulate its expression. This unique mechanism for ubiquitin ligases is responsible for regulating the tissue boundaries at the mesoderm/ectoderm border during embryogenesis.</P>
Antimorphic PV.1 Causes Secondary Axis by Inducing Ectopic Organizer
Hwang, Yoo-Seok,Seo, Jeong-Jae,Cha, Sang-Wook,Lee, Hyun-Shik,Lee, Sung-Young,Roh, Dong-Hyun,Kung, Hsiang-fu,Kim, Jaebong,Park, Mae Ja 경북대학교 병원 2002 경북대학교병원의학연구소논문집 Vol.6 No.1
Xenopus homeobox gene, PV.1 ventralizes activininduceddorsal mesoderm and inhibits neuralization of ectoderm in animal cap when overexpressed. Here we generated PV.1/engrailed fusion construct (N-PV1-EnR) to perform loss-of-function study for this transcription factor.N-PV1-EnR showed an extremely antimorphic effect, causing a partial secondary embryonic axis when expressed at ventral marginal zone of blastula. In ventral marginal zone cells, this chimeric protein induced organizer genes and suppressed ventral markers mimicking those effects reported for dominant negative BMP-4 receptor (DNBR).Moreover, N-PV1-EnR rescued the ventralized embryos caused by the ectopic dorsal expression of PV.1 but not by that of Xvent-2. These results suggested that PV.1 functions at downstream of BMP-4 as a ventralizing effector which acts separately from Xvent-2 and the dominant negative effect gained by this specific mutant is applicable for the further studiesof BMP-4 downstream pathway.
BMP 신호 하부 인자인 PV.1 단백질의 결합 단백질 동정
황유석(Yoo-Seok Hwang),채정필(Jeong-Pil Chae),김동선(Dong-Sun Kim),박권무(Kwon Moo Park),배용철(Yong Chul Bae),박매자(Mae Ja Park) 대한해부학회 2007 Anatomy & Cell Biology Vol.40 No.3
척추동물에서 BMP의 배(ventral)쪽 신호 전달 경로의 하위에 있는 전사조절인자들은 등배(dorsalventral)쪽 축을 따라 형성되는 중배엽에 있어서도 그 기능을 공유 한다는 것이 보고 되었다. 본 연구에서는 BMP 신호 물질의 하위 유전자인 PV.1 단백질이 배쪽과 등쪽에서의 신호전달에 있어서 차별적 기능을 알기 위하여 PV.1과 상호 결합하는 단백질을 검색하였다. 효모 이중 접합법을 이용하여 24개의 PV.1 결합 단백질을 검색하였고, 이중 결합하는 Xvent-2와 Xclaudin-6에 대한 PV.1 단백질의 도메인(domain) 연구를 하였다. PV.1 단백질의 C-terminus인 197-241 지역이 Xclaudin-6와 결합하는 반면에 Xvent-2는 PV.1 단백질의 C-terminus 전반에서 결합한다는 것을 확인하였다. 또한 PV.1과 결합하는 Xvent-2는 동형 이중 결합(Homodimerization)뿐만 아니라 PV.1과 결합하는 Xclaudin-6과도 이형 이중 결합(Heteodimerization)을 한다는 것을 확인하였다. Homeodomain transcription factors functioning downstream of BMP ventral pathway have been reported to share similar domain of roles in mesoderm patterning along the dorsal-ventral axis. To elucidate the differential role of PV.1 in the aspect of relationship between dorsal and ventral region, we tried to screen PV.1- interacting proteins. Twenty-four PV.1-interacting proteins were identified by yeast two-hybrid screening. Xvent-2 and Xclaudin-6 among these, went under domain study. The C-terminus of PV.1, more specifically 197-241 region was found to interact with Xclaudin-6. Meanwhile Xvent-2 has mild affinity to overall C-terminal region of PV.1. At the same time it was found that Xvent-2 homodimerizes and also binds to Xclaudin-6.
Lee, Sang Eun,Lee, Hae-Young,Cho, Hyun-Jai,Choe, Won-Seok,Kim, Hokon,Choi, Jin-Oh,Jeon, Eun-Seok,Kim, Min-Seok,Hwang, Kyung-Kuk,Chae, Shung Chull,Baek, Sang Hong,Kang, Seok-Min,Choi, Dong-Ju,Yoo, Byun American College of Cardiology 2017 JACC. Heart failure Vol.5 No.11
<P><B>Graphical abstract</B></P><P>[Figure]</P><P><B>Abstract</B></P><P><B>Objectives</B></P><P>This study aimed to assess the relationship between on-treatment blood pressure (BP) and clinical outcomes of patients with heart failure (HF).</P><P><B>Background</B></P><P>Lower BP has been reported to be related to increased mortality in various cardiovascular diseases. The optimal BP level for patients already experiencing HF is contentious.</P><P><B>Methods</B></P><P>The Korean Acute Heart Failure registry prospectively enrolled a total of 5,625 consecutive patients hospitalized for acute HF in 10 tertiary university hospitals in Korea between March 2011 and February 2014. Clinical profiles including BP were collected at admission, discharge, and during outpatient follow-up. Mean on-treatment BP was calculated from BP at discharge and at each follow-up visit. We evaluated the effects of mean on-treatment BP on the clinical outcomes of patients.</P><P><B>Results</B></P><P>Patients were followed up for a median 2.2 years. One-year mortality after discharge was 18.2%. The relationship between on-treatment BP and all-cause mortality followed a reversed J-curve relationship. A nonlinear, multivariable Cox proportional hazard model identified a nadir of systolic and diastolic BPs of 132.4/74.2 mm Hg in patients, for whom the mortality rate was lowest (p < 0.0001). The relationship with increased mortality above and below the reference BP was more definitive for diastolic BP and for HF with a preserved ejection fraction.</P><P><B>Conclusions</B></P><P>Systolic and diastolic BPs <130/70 mm Hg at discharge and during follow-up was associated with worse survival in HF patients. These data suggest that the lowest BP possible might not be an optimal target for HF patients. Further studies should establish a proper BP goal in HF patients. (Registry [Prospective Cohort] for Heart Failure in Korea [KorAHF]; NCT01389843)</P>
Yoo, Hyunhee,Moon, Seung-Kwan,Hwang, Taewon,Kim, Yong Seok,Kim, Joo-Hwan,Choi, Sung-Wook,Kim, Jung Hyun American Chemical Society 2013 Langmuir Vol.29 No.20
<P>This paper describes the preparation of magnetic nanoparticles modified with polyethylenimine (PEI)–folic acid (PF) conjugate and their potential biomedical applications. Magnetic nanoparticles modified with (3-(2-aminoethylamino)propyltrimethoxysilane) (AEAPS) were first prepared using a ligand exchange method to provide biocompatibility and hydrophilicity, and further conjugated with PF to carry gene and enhance specific uptake into cancer cells. We demonstrated the feasibility of the multifunctional magnetic nanoparticles as contrast agents in magnetic resonance imaging (MRI) and as gene carriers for gene delivery. In vitro results revealed that the cytotoxicity of the multifunctional magnetic nanoparticles was lower compared to that of pristine magnetic nanoparticles. Furthermore, we demonstrated the specific uptake of the magnetic nanoparticles modified with PF to KB cells using WI-38 cells as comparison by confocal microscopy. The PF-modified magnetic nanoparticles can potentially be employed as theranostic nanoplatforms for targeted gene delivery to cancer cells and simultaneous magnetic resonance imaging.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/langd5/2013/langd5.2013.29.issue-20/la3051302/production/images/medium/la-2012-051302_0008.gif'></P>
유경미,서우영,서한석,김완수,박재복,황인경 한국조리과학회 2004 한국식품조리과학회지 Vol.20 No.4
The purposes of this study were to investigate the physicochemical characteristics and storage stabilities of various sauces with added Yuza(Citrus Junos). Yuza consisted of three parts: peeled (46.17%), fleshed (43.74%) and seeded (10.09%). Yuza juice revealed 11.33% extraction yield, pH 2.85, 4.18% total acidity, 11° Brix, 1.16% transmittance and 2.63° Brix/acid ratio. Five kinds of sauces with added Yuza juice were Yuza red pepper paste sauce, Yuza soybean sauce for meat, Shahu shabu Yuza sauce, Korean Yuza vegetable sauce and Japanese Yuza soybean sauce. These sauces showed slight changes in pH, total acidity, total bacterial counts and overall sensory characteristics during storage period (60 days). In conclusion, Yuza juice could be applied to various sauces, which remained usable for 60 days.
( Yoo Jin Lee ),( Yu Jin Ha ),( Yu Na Kang ),( Koo Jeong Kang ),( Woo Jin Chung ),( Jae Seok Hwang ),( Byoung Kuk Jang ) 대한간학회 2013 춘·추계 학술대회 (KASL) Vol.2013 No.1
Patients and methods: Expression of autophagy related markers (LC3 and Beclin 1) and ER stress related markers (GRP78 and CHOP) were analyzed by immunohistochemistry on tissues from completely resected specimens of 190 HCC patients. Their influence on clinicopathologic features and prognosis were evaluated using Chi-square test and Kaplan-Meier analysis. Correlations of each proteins were determined by Spearman`s correlation analysis. Results: Expression of LC3 was not correlated with TNM, BCLC stage or Edmonson-Steiner grading, whereas, had correlation with longer overall survival (OS) (P=0.039) and longer disease free survival (DFS) (P=0.068) by Kaplan-Meier analysis. Multivariate analysis indicated that LC3 expression was significantly independent prognostic factors of OS (OR, 0.379; 95% CI, 0.192-0.748; P-value=0.005) and DFS (OR, 0.520; 95% CI, 0.299-0.843; P-value=0.009). Positive correlation was not observed in the expression of autophagy related markers with ER stress related markers. Conclusions: Our study showed that expression of LC3 was correlated with overall survival and disease free survival regardless of tumor staging or liver function state. These results suggest that expression and extent of LC3 might be a strong prognostic factor of HCC.
Case Report : Retroperitoneal Fibrosis in a Patient with Rheumatoid Arthritis
( Yoo Jeong Oh ),( Won Seok Lee ),( Mi Hee Kang ),( Joo Hee Hwang ),( Wan Hee Yoo ) 대한류마티스학회 2015 대한류마티스학회지 Vol.22 No.6
A 54-year-old male diagnosed with rheumatoid arthritis (RA) was effectively treated with methotrexate and adalimumab. He was admitted with fatigue and right lower back pain which had persisted for 1 month. An enhanced abdominal computed tomography scan showed an ill-defined mass with soft tissue attenuation surrounding the right common iliac artery involving the right middle portion of the ureter. Laparoscopic ureterolysis and biopsy were performed. Microscopic evaluation confirmed the presence of fibroblastic proliferation, with a pleomorphic inflammatory cell infiltrate consisting predominantly of lymphocytes, macrophages, and vascular endothelial cells, without granuloma or neoplastic cells. Therefore, our diagnosis was retroperitoneal fibrosis (RPF)-associated RA. Clinicians should consider the possibility of RPF in patients with RA who experience lower back pain, abdominal pain, or dysuria, and order suitable imaging studies. (J Rheum Dis 2015;22:391-394)