The Clinical, Molecular, and Mechanistic Basis of RUNX1 Mutations Identified in Hematological Malignancies

Yokota, Asumi,Huo, Li,Lan, Fengli,Wu, Jianqiang,Huang, Gang Korean Society for Molecular and Cellular Biology 2020 Molecules and cells Vol.43 No.2

RUNX1 plays an important role in the regulation of normal hematopoiesis. RUNX1 mutations are frequently found and have been intensively studied in hematological malignancies. Germline mutations in RUNX1 cause familial platelet disorder with predisposition to acute myeloid leukemia (FPD/AML). Somatic mutations of RUNX1 are observed in various types of hematological malignancies, such as AML, acute lymphoblastic leukemia (ALL), myelodysplastic syndromes (MDS), myeloproliferative neoplasm (MPN), chronic myelomonocytic leukemia (CMML), and congenital bone marrow failure (CBMF). Here, we systematically review the clinical and molecular characteristics of RUNX1 mutations, the mechanisms of pathogenesis caused by RUNX1 mutations, and potential therapeutic strategies to target RUNX1-mutated cases of hematological malignancies.

Psychological dependence in Japanese animation films: A case of Rin Taro

Yokota, Masao,Koide, Masashi,Nomura, Koji Korean Society of Cartoon and Animation Studies 2004 만화애니메이션연구 Vol.- No.8

There are many opportunistic events that accidentally happen in Rin Taro's animations. However, audiences usually enjoy them in which dramatic catastrophic scenes inflict destruction on a city. Every acquaintance that a hero meets helps him to complete his wish in <Galaxy Express 999>. Rin's animations describe the hero as a person that every acquaintance is eager to help. This means that the fulfillment of hero's wish depends on sympathy of the other persons toward the hero. Thus, the hero is psychologically dependent on the others. However, Rin Taro gradually became to describe destructive aspects of psychological dependence. A female character in 'X' has a symbiotic relationship with a computer that has a human-like jealousy. At last, it kills her from its jealousy. Rin Taro had shifted from helpful psychological dependence to destructive one in his animations. Therefore, Rin Taro described Japanese mental state as psychological dependence in his animations in accordance with the present state in Japan.

Ductility of open piled wharves under reversed cyclic loads

Yokota, Hiroshi,El-Bakry, Hazem M.F. Techno-Press 2001 Structural Engineering and Mechanics, An Int'l Jou Vol.12 No.6

Ductility of open piled wharves under reversed cyclic loads has been investigated. Experimental testing of five wharf models having a scale of about 1:4 was conducted under the application of horizontal reversed cyclic loading. The experiments were designed to focus on the horizontal ultimate load, ductility and failure mode of the considered wharf models. Nonlinear numerical analyses using the finite element method were also performed on numerical models representing the experimentally tested wharves. The results of the experimental tests showed that open piled wharves possessed favourable ductile behaviour and that their load bearing capacity did not depreciate until a ductility factor of 3 to 4 was reached. The numerical analysis showed that the relative rotation that took place at the joints between the steel piles and the R.C. beam was responsible for a considerable portion of the total horizontal deformation of the wharves. Therefore, it was concluded that introducing the joint stiffness in calculating the deformations of open piled wharves was important to achieve reasonable accuracy.

DIGESTION OF NAPIERGRASS SILAGE BY GOATS

Yokota, H.,Ohshima, M.,Okajima, T.,Ito, Y. Asian Australasian Association of Animal Productio 1989 Animal Bioscience Vol.2 No.3

NUTRITIONAL QUALITY OF WILTED NAPIER GRASS (Pennisetum purpureum Schum.) ENSILED WITH OR WITHOUT MOLASSES

Yokota, H.,Kim, J.H.,Okajima, T.,Ohshima, M. Asian Australasian Association of Animal Productio 1992 Animal Bioscience Vol.5 No.4

To investigate the effects of molasses addition at ensiling on nutritional quality of wilted napier grass, chemical quality and nutrient composition of the silages, digestibility and nitrogen retention at feeding trials were analysed using 4 goats in a cross over design. The results are as follows : 1. Molasses addition at ensiling decreased pH value (3.99) and ammonia nitrogen, and increased lactic acid content by 285% compared to non-additive silage (83.5 g/kg dry matter). 2. There were no differences in digestibilities of dry matter, crude protein, neutral detergent fiber, acid detergent fiber, hemicellulose and cellulose between the silage ensiled with molasses (MS silage) and the silage ensiled without molasses (WS silage). Urinary nitrogen excretion, however, significantly (p<0.05) decreased in goats fed the MS silage, and nitrogen retention was positive in goats fed the MS silages, but negative in goats fed the WS silage. 3. Acetic acid concentration in remained fluids in goats fed the MS silage was lower and propionic and butyric acid concentrations were higher than those in goats fed the WS silage. As water soluble carbohydrate content was higher in the MS silage than in the WS silage, a part of added molasses was still remained in the silage at the feeding trials and could be utilized for energy sources by the goats. Nitrogen may be also effectively utilized in goats fed the MS silage, because the silage were inhibited in proteolysis during ensiling.

Influence of water content in sub-flooring materials using adhesive on chemical compounds emission

Yokota, Tomohiro,Kato, Shinsuke,Seo, Janghoo,Chino, Satoko,Kim, Jonghun TAYLOR FRANCIS 2013 Journal of adhesion science and technology Vol.27 No.5

Nutritional Quality of Napier Grass (Pennisetum purpureum Schum.) Silage Supplemented with Molasses and Rice Bran by Goats

Yokota, H.,Fujii, Y.,Ohshima, M. Asian Australasian Association of Animal Productio 1998 Animal Bioscience Vol.11 No.6

In order to improve silage quality and utilization of napier grass (Pennisetum purpureum Schum.) by goats, the grass was ensiled with molasses (MOL) and/or defatted rice bran (DRB). Napier grass was harvested at the growing stage in July and cut into 3 cm length. The grass was mixed with 4% MOL and/or 15% DRB, ensiled 15 kg each into plastic bags and stored for 9 months. Dry matter content of the silage ensiled with MOL (MOL-silage) was 13.4%, but increased to 20% with DRB addition. The addition of MOL decreased pH value and ammonia nitrogen content, but increased lactic acid content. MOL-silage contained about 6% spoilage, but addition of DRB decreased spoilage to less than 1%. Goats were fed the silage at a level of 2.25% (DM basis) of their body weight. Goats fed DRB- or MOL/DRB-silages maintained nitrogen retention, but goats fed MOL-silage did not. The rumen fluid of goats fed DRB-silage tended to be higher in acetic acid and lower in propionic acid than those fed the other silages. Ammonia in the rumen fluids, urea nitrogen in the blood and the urinary nitrogen excretion were the lowest in goats fed MOL/DRB-silage. As the result, the ratio of retained nitrogen to nitrogen intake was the highest in goats fed MOL/DRB-silage. In conclusion, addition of DRB to napier grass increased DM of silage and decreased the volume of spoilage. The combination of MOL and DRB can improve the fermentation quality and thus enhance the utilization of the silage by goats, more than the MOL or DRB being as a single treatment.

EFFECT OF HARVEST INTERVALS ON THE CHEMICAL COMPOSITION AND NUTRITIVE VALUE OF NAPIER GRASS (Pennisetum purpureum Schum.) SILAGES FOR GOATS

Yokota, H.,Okajima, T.,Ohshima, M. Asian Australasian Association of Animal Productio 1994 Animal Bioscience Vol.7 No.4

Chemical composition and nutritive values of napier grass (Pennisetum purpureum Schum.) silages subjected to two cutting intervals were studies; 1st harvest in July (A), and 2nd (B) and 1st (C) harvests in November. Each forage was ensiled with 4% molasses in plastic bags and stored for 5 or 9 months. A feeding experiment with castrated goats was conducted from April to June of the following year. Dry matter (DM) and crude protein (CP) content of the harvests varied from 9.5 to 22.8% and 6.6 to 13.6% of DM, respectively. The dry matter content of the silages fed to the goats were 13.0 to 24.4%, because some effluent was removed from each silage before the feeding trial. The pH values of the silages were between 4.03 and 4.29. Goats were given sufficient silage to meet maintenance nitrogen requirements from napier grass silage. Silage C was not completely consumed, and the silage had low digestibilities of DM, CP, hemicellulose and cellulose. Nitrogen balance was slightly positive for goats consuming silage B and was negative for goats consuming silages A and C. Nitrogen utilization was discussed in terms of ruminal $NH_3-N$ and volatile fatty acid concentration in the rumen fluids. It is concluded that goats could not maintain N-equilibrium not only when a younger forage was consumed at a level of N requirement by a restricted feeding, but also when an older forage could not be consumed enough for N requirement because of feed intake limitation.

EFFECT OF ENVIRONMENTAL TEMPERATURE AND ADDITION OF MOLASSES ON THE QUALITY OF NAPIER GRASS (PENNISETUM PURPUREUM SCHUM.) SILAGE

Yokota, H.,Okajima, T.,Ohshima, M. Asian Australasian Association of Animal Productio 1991 Animal Bioscience Vol.4 No.4

The effect of molasses addition and hot temperature on the ensiling characteristics of napier grass (Pennisetum purpureum Schum.) were studied. Napier grass was harvested five times at intervals from 22 to 39 days and each harvest was divided into two equal portions. The half portion was ensiled directly and the other half was ensiled after mixing with molasses into polyethylene bag silos of 15 kg capacity. Molasses was added at the rate of 4% of fresh weight of the grass. One half of the each treatment was conserved at a room of $40^{\circ}C$ for a month and then moved to an ambient temperature room. The other half was kept at ambient temperature for the whole experimental duration. The silages were opened 3 to 7 months after ensiling. Addition of molasses enhanced lactic acid fermentation by increasing lactic acid content and reducing pH value, ammonia nitrogen and acetic, propionic and butyric acid contents of the silages in both temperature treatments. Enhanced temperature increased pH value and decreased acetic, propionic and butyric acids.

A 3D ground penetrating radar imaging of the heavy rainfall-induced deformation around a river levee: a case study of Ara River, Saitama, Japan

Yokota, Toshiyuki,Inazaki, Tomio,Shinagawa, Shunsuke,Ueda, Takumi Korean Society of Earth and Exploration Geophysici 2009 지구물리와 물리탐사 Vol.12 No.1

This paper describes a three-dimensional ground penetrating radar (GPR) survey carried out around a levee of the Ara River in Saitama, Japan, where deformation of the ground was observed after heavy rainfall associated with the typhoon of September 2007. The high-density 3D GPR survey was conducted as a series of closely adjacent four directional sets of 2D surveys at an area surrounding vertical cracks on the paved road caused by deformations induced by heavy rain. The survey directions of the 2D surveys were 0, 90, 45, and -45 degrees with respect to the paved road and the intervals between lines were less than 0.5 m. The 3D subsurface structure was accurately imaged by the result of data processing using Kirchhoff-type 3D migration. As a result, locations and vertical continuities of the heavy rainfall induced cracks in the paved road were clearly imaged. This will be a great help in considering the generation mechanisms of the cracks. Moreover, the current risk of a secondary disaster was found to be low, as no air-filled cavities were detected by the 3D GPR survey.