Effect of anti-skin disorders of ginsenosides- A Systematic Review

Lele Cong,Jinli Ma,Yundong Zhang,Yifa Zhou,Xianling Cong,Miao Hao 고려인삼학회 2023 Journal of Ginseng Research Vol.47 No.5

Ginsenosides are bioactive components of Panax ginseng with many functions such as anti-aging, antioxidation,anti-inflammatory, anti-fatigue, and anti-tumor. Ginsenosides are categorized into dammarane,oleanene, and ocotillol type tricyclic triterpenoids based on the aglycon structure. Based on thesugar moiety linked to C-3, C-20, and C-6, C-20, dammarane type was divided into protopanaxadiol (PPD)and protopanaxatriol (PPT). The effects of ginsenosides on skin disorders are noteworthy. They play antiagingroles by enhancing immune function, resisting melanin formation, inhibiting oxidation, andelevating the concentration of collagen and hyaluronic acid. Thus, ginsenosides have previously beenwidely used to resist skin diseases and aging. This review details the role of ginsenosides in the anti-skinaging process from mechanisms and experimental research.

The effective mechanism of the polysaccharides from Panax ginseng on chronic fatigue syndrome

Wang, Jia,Sun, Chengxin,Zheng, Yan,Pan, Hongling,Zhou, Yifa,Fan, Yuying 대한약학회 2014 Archives of Pharmacal Research Vol.37 No.4

Ginseng acidic polysaccharide WGPA isolated from the root of Panax ginseng C. A. Meyer was fractionated into WGPA-A and WGPA-N by anion-exchange chromatography. The antifatigue activity of ginseng acidic polysaccharide WGPA has been reported in our previous research. This present study was designed to identify its active component and elucidate the mechanism for preventing chronic fatigue syndrome (CFS). WGPA, WGPA-A and WGPA-N were orally administered to mice once daily for 15 days. The effects of these compounds on physiological biomarkers of oxidative stress and on the morphology of the mitochondria in striated skeletal muscle were assessed. The results of forced swimming test-induced indicated that WGPA and WGPA-A could lengthen the swimming time, while WGPA-N could not. In addition, malondialdehyde and lactate dehydrogenase levels in serum were enhanced; while those of superoxide dismutase and glutathione peroxidase were lowered. Interestingly, the structural degeneration of mitochondria were all ameliorated. These findings suggested that WGPA-A is the active component of WGPA, it might have potential therapeutic effects for CFS and the oxidative stress might be involved in the pathogenesis. Our results also provided essential data for a better understanding of the antifatigue effects of P. ginseng extracts.

Antitumor Activities and Immunomodulatory Effects of Ginseng Neutral Polysaccharides in Combination with 5-Fluorouracil

Weihua Ni,Xu Zhang,Bo Wang,Yan Chen,Han Han,Yuying Fan,Yifa Zhou,Guihua Tai 한국식품영양과학회 2010 Journal of medicinal food Vol.13 No.2

A neutral polysaccharide fraction (WGPN) prepared from Panax ginseng C.A. Meyer by hot water extraction and DEAE-cellulose chromatography was tested for its anticancer activity alone and in combination with 5-fluorouracil (5-FU) in Sarcoma-180 (S180) tumor-bearing mice by intragastric administration. WGPN alone inhibited S180 tumor growth in a bell-shaped dose–response curve, and the combination with 5-FU showed a synergistic effect. Studies of various immunological activities in S180-bearing mice revealed that WGPN stimulated the proliferation of lymphocytes, increased natural killer cell cytotoxicity, enhanced the phagocytosis and nitric oxide production by macrophages, and increased the level of tumor necrosis factor-α in serum. In combination with 5-FU, WGPN mitigated damage to the immune system caused by 5-FU in S180-bearing mice. These results suggest that WGPN might be a potential adjuvant for chemotherapeutic drugs.

The effective mechanism of the polysaccharides from Panax ginseng on chronic fatigue syndrome

Jia Wang,Chengxin Sun,Yan Zheng,Hongling Pan,Yifa Zhou,Yuying Fan 대한약학회 2014 Archives of Pharmacal Research Vol.37 No.4

Ginseng acidic polysaccharide WGPA isolatedfrom the root of Panax ginseng C. A. Meyer was fractionatedinto WGPA-A and WGPA-N by anion-exchangechromatography. The antifatigue activity of ginseng acidicpolysaccharide WGPA has been reported in our previousresearch. This present study was designed to identify itsactive component and elucidate the mechanism for preventingchronic fatigue syndrome (CFS). WGPA, WGPAAand WGPA-N were orally administered to mice oncedaily for 15 days. The effects of these compounds onphysiological biomarkers of oxidative stress and on themorphology of the mitochondria in striated skeletal musclewere assessed. The results of forced swimming testinducedindicated that WGPA and WGPA-A couldlengthen the swimming time, while WGPA-N could not. Inaddition, malondialdehyde and lactate dehydrogenase levelsin serum were enhanced; while those of superoxidedismutase and glutathione peroxidase were lowered. Interestingly, the structural degeneration of mitochondriawere all ameliorated. These findings suggested thatWGPA-A is the active component of WGPA, it might havepotential therapeutic effects for CFS and the oxidative stress might be involved in the pathogenesis. Our resultsalso provided essential data for a better understanding ofthe antifatigue effects of P. ginseng extracts.

Molecular Cloning and Characterization of a Novel Exo-β-1,3-Galactanase from Penicillium oxalicum sp. 68

Zhou, Tong,Hu, Yanbo,Yan, Xuecui,Cui, Jing,Wang, Yibing,Luo, Feng,Yuan, Ye,Yu, Zhenxiang,Zhou, Yifa The Korean Society for Microbiology and Biotechnol 2022 Journal of microbiology and biotechnology Vol.32 No.8

Arabinogalactans have diverse biological properties and can be used as pharmaceutical agents. Most arabinogalactans are composed of β-(1→3)-galactan, so it is particularly important to identify β-1,3-galactanases that can selectively degrade them. In this study, a novel exo-β-1,3-galactanase, named PoGal3, was screened from Penicillium oxalicum sp. 68, and hetero-expressed in P. pastoris GS115 as a soluble protein. PoGal3 belongs to glycoside hydrolase family 43 (GH43) and has a 1,356-bp gene length that encodes 451 amino acids residues. To study the enzymatic properties and substrate selectivity of PoGal3, β-1,3-galactan (AG-P-I) from larch wood arabinogalactan (LWAG) was prepared and characterized by HPLC and NMR. Using AG-P-I as substrate, purified PoGal3 exhibited an optimal pH of 5.0 and temperature of 40℃. We also discovered that Zn²⁺ had the strongest promoting effect on enzyme activity, increasing it by 28.6%. Substrate specificity suggests that PoGal3 functions as an exo-β-1,3-galactanase, with its greatest catalytic activity observed on AG-P-I. Hydrolytic products of AG-P-I are mainly composed of galactose and β-1,6-galactobiose. In addition, PoGal3 can catalyze hydrolysis of LWAG to produce galacto-oligomers. PoGal3 is the first enzyme identified as an exo-β-1,3-galactanase that can be used in building glycan blocks of crucial glycoconjugates to assess their biological functions.

The Inhibitory Effect of Ginseng Pectin on L-929 Cell Migration

Yuying Fan,Hairong Cheng,Dan Liu,Xu Zhang,Bo Wang,Lin Sun,Guihua Tai,Yifa Zhou 대한약학회 2010 Archives of Pharmacal Research Vol.33 No.5

We tested the effects of ginseng pectin prepared by enzymatic hydrolysis of ginseng polysaccharides on cell migration. Ginseng pectin impaired the migration of L-929 cells and reduced their migration speed by up to 50% of control in the presence or absence of serum, suggesting it worked on both serum-dependent and serum-independent migration pathways. Ginseng pectin impaired cell migration via decreased cell spreading. These findings represent a significant contribution towards understanding the bioactivities of ginseng polysaccharides and applying them to health food and medicine.

Antiarrhythmic effects of ginsenoside Rg2 on calcium chloride-induced arrhythmias without oral toxicity

Dongxia Gou,Xuejing Pei,Jiao Wang,Yue Wang,Chenxing Hu,Chengcheng Song,Sisi Cui,Yifa Zhou 고려인삼학회 2020 Journal of Ginseng Research Vol.44 No.5

Background: Malignant arrhythmias require drug therapy. However, most of the currently available antiarrhythmic drugs have significant side effects. Ginsenoside Rg2 exhibits excellent cardioprotective effects and appears to be a promising candidate for cardiovascular drug development. So far, the oral toxicity and antiarrhythmic effects of Rg2 have not been evaluated. Methods: Acute oral toxicity of Rg2 was assessed by the Limit Test method in mice. Subchronic oral toxicity was determined by repeated dose 28-day toxicity study in rats. Antiarrhythmic activities of Rg2 were evaluated in calcium chloride-induced arrhythmic rats. Antiarrhythmic mechanism of Rg2 was investigated in arrhythmic rats and H9c2 cardiomyocytes. Results: The results of toxicity studies indicated that Rg2 exhibited no single-dose (10 g/kg) acute oral toxicity. And 28-day repeated dose treatment with Rg2 (1.75, 3.5 and 5 g/kg/d) demonstrated minimal, if any, subchronic toxicity. Serum biochemical examination showed that total cholesterol in the high-dose cohort was dramatically decreased, whereas prothrombin time was increased at Day 28, suggesting that Rg2 might regulate lipid metabolism and have a potential anticoagulant effect. Moreover, pretreatment with Rg2 showed antiarrhythmic effects on the rat model of calcium chloride induced arrhythmia, in terms of the reduced duration time, mortality, and incidence of malignant arrhythmias. The antiarrhythmic mechanism of Rg2 might be the inhibition of calcium influx through L-type calcium channels by suppressing the phosphorylation of Ca<SUP>2+</SUP>/calmodulin-dependent protein kinase II. Conclusion: Our findings support the development of Rg2 as a promising antiarrhythmic drug with fewer side effects for clinical use.

Ginseng-derived type I rhamnogalacturonan polysaccharide binds to galectin-8 and antagonizes its function

Yi Zheng,Yunlong Si,Xuejiao Xu,Hongming Gu,Zhen He,Zihan Zhao,Zhangkai Feng,Jiyong Su,Kevin H. Mayo,Yifa Zhou,Guihua Tai The Korean Society of Ginseng 2024 Journal of Ginseng Research Vol.48 No.2

Background: Panax ginseng Meyer polysaccharides exhibit various biological functions, like antagonizing galectin-3-mediated cell adhesion and migration. Galectin-8 (Gal-8), with its linker-joined N- and C-terminal carbohydrate recognition domains (CRDs), is also crucial to these biological processes, and thus plays a role in various pathological disorders. Yet the effect of ginseng-derived polysaccharides in modulating Gal-8 function has remained unclear. Methods: P. ginseng-derived pectin was chromatographically isolated and enzymatically digested to obtain a series of polysaccharides. Biolayer Interferometry (BLI) quantified their binding affinity to Gal-8, and their inhibitory effects on Gal-8 was assessed by hemagglutination, cell migration and T-cell apoptosis. Results: Our ginseng-derived pectin polysaccharides consist mostly of rhamnogalacturonan-I (RG-I) and homogalacturonan (HG). BLI shows that Gal-8 binding rests primarily in RG-I and its β-1,4-galactan side chains, with sub-micromolar K_D values. Both N- and C-terminal Gal-8 CRDs bind RG-I, with binding correlated with Gal-8-mediated function. Conclusion: P. ginseng RG-I pectin β-1,4-galactan side chains are crucial to binding Gal-8 and antagonizing its function. This study enhances our understanding of galectin-sugar interactions, information that may be used in the development of pharmaceutical agents targeting Gal-8.