Broad-specificity amino acid racemase, a novel non-antibiotic selectable marker for transgenic plants

Yi‑Chia Kuan,Venkatesan Thiruvengadam,Jia‑Shin Lin,Jia‑Hsin Liu,Tsan‑Jan Chen,Hsin‑Mao Wu,Wen‑Ching Wang,Liang‑Jwu Chen 한국식물생명공학회 2018 Plant biotechnology reports Vol.12 No.1

The broad-specificity amino acid racemase (Bsar) from Pseudomonas putida catalyzes the racemization of various amino acids, offering a flexible and feasible platform to develop a new non-antibiotic selectable marker system for plant transformation. In the present study, we demonstrated that a Bsar variant, Bsar-R174K, that is useful as a selectable marker gene in Arabidopsis and rice that were susceptible to l-lysine and D-alanine. The introduction of wild-type Bsar, Bsar-R174K or Bsar-R174A into E. coli lysine or asparagine auxotrophs was able to rescue the growth of these microorganisms in minimal media supplemented with selectable amino acid enantiomers. The transformation of Arabidopsis with Bsar or Bsar variants based on d-alanine selection revealed that Bsar-R174K had the greatest efficiency (2.40%), superior to kanamycin selectionbased transformation (1.10%). Whereas, l-lysine-based selection exhibited lower efficiency for Bsar-R174K (0.17%). The progenies of selected Bsar-R174K transgenic Arabidopsis revealed normal growth properties. In addition, Bsar-R174K transgenic rice was obtained on l-lysine medium with an efficiency of 0.9%, and the progenies of the transgenic rice revealed morphologically normal phenotypes comparable with their wild-type counterparts. This study presents the first report of broad range amino acid racemase Bsar-R174K as a non-antibiotic selectable marker system applied in transgenic plants.

The Development and Current State of Inclusive Education at Universities in Taiwan

Hsin Yi Wang 아시아지적장애인연맹 산하 아시아저널센터 2019 Asia-Pacific Journal of Intellectual Disabilities Vol.6 No.2

The purpose of this study is to investigate the development of current state of inclusive education at universities in Tiwan. In Tiwan, inclusive education is for all students who are disabled/non-disabled. We believe that inclusive education ensure the educational right of all students regardless of the type and degree of individual difference. However, challenges remain in the inclination of teaching staff to modify their teaching materials, as this is currently not in line with the United Nations Convention on the Rights of Persons with Disabilities (CRPD) obligations. Furthermore, higher education is not part of compulsory education, a fact which can be a point of debate and represent a challenge to implementing inclusive education in this setting. In conclusion, students with developmental disabilities who cannot comprehend the theoretical or academic subjects being studied in higher education may be better served by appropriate transfers or bridge courses aimed to deliver career-oriented knowledge and training.

全求化與中國當代文藝狀況

王一川 中國語文硏究會 2003 中國語文論叢 Vol.24 No.-

본 논문은 세계화 맥락 속에서 중국의 문예현황을 논술하고 있다. 세계화는 단순히 세계의 일체화를 가리키는 것이 아니라, 세계의 일체화, 세계체계론과 사회전환론 등 세 종류의 의의를 함축하고 있어 세계화에 대한 단편적인 오해를 바로 잡을 필요가 있다. 세계화는 실제적으로 현대인의 전세계적 공생과 상호적인 체험을 가리키는 것으로 16세기 이래 세계 각 민족간의 상호의존과 삼투가 나날이 긴밀해지는 생활방식이며, 특정지역의 생존체험과 멀리 떨어진 지역의 생존체험이 이러 저러한 연관을 맺는 것을 의미한다. 이러한 세계화의 맥락 속에서 예술을 고찰하기 위해서는 생산과 소비영역에서의 예술의 종족, 매개, 과학기술, 금융과 이데올로기 등 다층적인 상황을 고려해야 하며, 예술의 주류문화, 고급문화, 대중문화와 민간문화 등 다양한 형태간의 다원적인 상호삼투상황, 그리고 새로운 다원화된 변화생성의 양태를 살펴보아야 한다.

半夏瀉心湯이 CCl4 로 유도된 간중독 흰쥐에 미치는 영향

朱旺錫,朴賢俊,尹炳局,鄭成伊,朴宣東 대한본초학회 1999 大韓本草學會誌 Vol.14 No.2

The purpose of this study is to observe the effect of Banhasasimtang on serum reaction in CCI₄ treated rats. In this study the experimental rats divided five group(Normal, Control, Sample A, Sample B and Sample C group) Under the same condition Normal group was fed basal diet and water, Control group was injected carbon tetrachloride(CCI₄ 0.5㎖/㎏) and basal diet for 2 weeks, Sample A group was injected carbon tetrachloride(CCI₄0.5㎖/㎏) and fed the Bahasasimtang extract(10㎖/㎏) for 2 weeks, Sample A group was injected carbon tetrachloride(CCI₄ 0.5㎖/㎏) and fed the Banhasasimtang extract(10㎖/㎏) for 2 weeks, Sample B group was fed the Banhasasimtang extract(10㎖/㎏) for 2 weeks and injected carbon tetrachloride(CCI₄ 0.5㎖/㎏), Sample C group was fed the Banhasasimtang extract(10㎖/㎏) for 2 weeks. The change of GOT, GPT, γ-GPT, ALP, LDH activity and Bilirubin level in blood serum. The obtained results are summarized as follows : 1. In the change of GOT GPT contents, as compared with control group, sample group was significantly decreased. 2. In the change of γ-GPT contents, as compared wth control group, sample group was significantly decreased. 3. In the change of ALP contents, as compared with control group, sample group was significantly decreased. 4. In the change of LDP contents, as compared with control group, sample group was significantly decreased. 5. In the change of Bilirubin contents, as compared with control group, sample group was significantly decreased.

Application of a traction metal clip with a fishhook-like device in wound sutures after endoscopic resection

Wang Fangjun,Leng Xia,Gao Yi,Shen Xiuyun,Wang Wenping,Liu Huamin,Liu Pengfei 대한소화기내시경학회 2022 Clinical Endoscopy Vol.55 No.4

Background/Aims: Endoscopic wound suturing is an important factor that affects the ability to remove large and full-thickness lesionsduring endoscopic resection. We aimed to evaluate the effect of a traction metal clip with a fishhook-like device on wound sutures afterendoscopic resection. Methods: From July 2020 to April 2021, patients who met the enrollment criteria were treated with a fishhook-like device during theoperation to suture the postoperative wound (group A). Patients with similar conditions and similar size wounds who were treatedwith a “purse-string suture” to suture the wounds were retrospectively analyzed as the control group (group B). Difference in the suturerate, adverse events, time required for suturing, and number of metal clips were compared between the two groups. Results: The time required for suturing was 7.72±0.51 minutes in group A and 11.50±0.91 minutes in group B. This difference was statisticallysignificant (F=13.071, p=0.001). The number of metal clamps used in group A averaged 8.1 pieces/case, and the number ofmetal clamps used in group B averaged 7.3 pieces/case. This difference was not statistically significant (F=0.971, p=0.331). Conclusions: The traction metal clip with the fishhook-like device is ingeniously designed and easy to operate. It has a good suture effecton the wound after endoscopic submucosal dissection and effectively prevents postoperative adverse events.

Downregulation of Orai1 expression in the airway alleviates murine allergic rhinitis

Yi Wang,Lin Lin,Chunquan Zheng 생화학분자생물학회 2012 Experimental and molecular medicine Vol.44 No.3

Orai1 is the key subunit of the Ca2+-release-activated Ca2+ channel. Our previous report has demonstrated that Orai1 expression in the airway was upregulated in the ovalbumin (OVA)-induced allergic rhinitis (AR)mouse models. To observe whether inhibition of Orai1expression in the airway could suppress symptoms in a murine model of AR and to assess the impacts of this inhibition on the responses of local and systemic immunocytes,we administered recombinant lentivirus vectors that encoded shRNA against ORAI1 (lenti-ORAI1)into the nostrils of OVA-sensitized mice before the challenges, and analyzed its effect on allergic responses,as compared with the unsensitized mice and untreated AR mice. Administration of lenti-ORAI1 into the nasal cavity successfully infected cells in the epithelial layer of the nasal mucosa, and significantly decreased the frequencies of sneezing and nasal rubbing of the mice. Protein levels of leukotriene C4, OVA-specific IgE, and IL-4 in the nasal lavage fluid and serum and eosinophil cation protein in the serum were also significantly reduced by lenti-ORAI1, as were the mRNA levels of these factors in the nasal mucosa and spleen. These data suggested that administration of lenti-ORAI1 into the nasal cavity effectively decreased Orai1 expression in the nasal mucosa, alleviated AR symptoms, and partially inhibited the hyperresponsiveness of the local and systemic immune cells including T cells, B cells, mast cells and eosinophils that are involved in the pathogenesis of AR.

Optimization of Brazing Conditions for OFHC Cu and ASTM A501 Low Carbon Steel by Taguchi Method

Yi Qi Wang,Ali Md. Afsar,송정일 한국정밀공학회 2009 International Journal of Precision Engineering and Vol. No.

This paper concerns the optimization of brazing conditions for joining the two dissimilar materials Oxygen-free high conductivity (OFHC) copper and ASTM A501 low carbon steel usually used for an Oil-Separator of an airconditioner using Ag-based (BAg) alloy as a brazing filler metal (BFM). A mixture of 70% N2 and 30% H2 gases was used to prevent oxidation of the joints during furnace brazing process. Brazing joint clearance, length, temperature, and time were selected as design parameters that have significant effect on the bonding strength of a brazed joint. Taguchi method was used as a statistical technique for design of experiment (DOE) in an attempt to optimize brazing conditions in terms of shear strength. L9 (34) orthogonal array was designed for conducting the experiments. The relative influence of design parameters and their interaction on the response were also discussed. The experimental results verified that the brazing conditions predicted in this study by Taguchi method could produce the brazed joint between OFHC copper and ASTM A501 low carbon steel with maximum shear strength.

Three New Non-reducing Polyketide Synthase Genes from the Lichen-Forming Fungus Usnea longissima

( Yi Wang ),( Juan Wang ),( Yong Hwa Cheong ),( Jae Seoun Hur ) 한국균학회 2014 Mycobiology Vol.42 No.1

Usnea longissima has a long history of use as a traditional medicine. Several bioactive compounds, primarily belongingto the polyketide family, have been isolated from U. longissima. However, the genes for the biosynthesis of these compounds areyet to be identified. In the present study, three different types of non-reducing polyketide synthases(UlPKS2, UlPKS4, andUlPKS6) were identified from a cultured lichen-forming fungus of U. longissima. Phylogenetic analysis of product templatedomains showed that UlPKS2 and UlPKS4 belong to group IV, which includes the non-reducing polyketide synthases with anmethyltransferase(MeT) domain that are involved in methylorcinol-based compound synthesis; UlPKS6 was found to belong togroup I, which includes the non-reducing polyketide synthases that synthesize single aromatic ring polyketides, such as orsellinicacid. Reverse transcriptase-PCR analysis demonstrated that UlPKS2 and UlPKS4 were upregulated by sucrose; UlPKS6 wasdownregulated by asparagine, glycine, and alanine.

[Story by Yi Kwang-su] The Unenlightened (Ⅲ)

Yi Kwang-su,Chang Wang-rok(번역자) The Academy of Korean Studies 1970 Korea Journal Vol.10 No.5

Prognostic Threshold of Neuroendocrine Differentiation in Gastric Carcinoma: a Clinicopathological Study of 945 Cases

Yi Zou,Linying Chen,Xingfu Wang,Yupeng Chen,Liwen Hu,Saifan Zeng,Pengcheng Wang,Guoping Li,Ming Huang,Liting Wang,Shi He,Sanyan Li,Lihui Jian,Sheng Zhang 대한위암학회 2019 Journal of gastric cancer Vol.19 No.1

Purpose: The significance of neuroendocrine differentiation (NED) in gastric carcinoma (GC) is controversial, leading to ambiguous concepts in traditional classifications. This study aimed to determine the prognostic threshold of meaningful NED in GC and clarify its unclear features in existing classifications. Materials and Methods: Immunohistochemical staining for synaptophysin, chromogranin A, and neural cell adhesion molecule was performed for 945 GC specimens. Survival analysis was performed using the log-rank test and univariate/multivariate models with percentages of NED (PNED) and demographic and clinicopathological parameters. Results: In total, 275 (29.1%) cases were immunoreactive to at least 1 neuroendocrine (NE) marker. GC-NED was more common in the upper third of the stomach. PNED, and Borrmann's classification and tumor, lymph node, metastasis stages were independent prognostic factors. The cutoff PNED was 10%, beyond which patients had significantly worse outcomes, although the risk did not increase with higher PNED. Tumors with ≥10% NED tended to manifest as Borrmann type III lesion with mixed/diffuse morphology and poorer histological differentiation; the NE components in this population mainly grew in insulae/nests, which differed from the predominant growth pattern (glandular/acinar) in GC with <10% NED. Conclusions: GC with ≥10% NED should be classified as a distinct subtype because of its worse prognosis, and more attention should be paid to the necessity of additional therapeutics for NE components.