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배추(Brassica campestris ssp. napus var. pekinensis)의 캘러스 배양을 통한 식물체 재분화
이숙이,박민철 가톨릭대학교 자연과학연구소 1998 자연과학논문집 Vol.19 No.-
배추(Brassica campestris ssp. napus var. pekinensis)의 기내 조직배양에서 캘러스 형성 및 식물체 재분화에 미치는 생장조절제의 영향과 AgNO₃의 효과를 조사하였다. ‘삼진’과 ‘서울’ 배추를 이용하여 발아 6일된 유식물의 자엽과 배축으로부터 캘러스를 유도하였다. 캘러스의 형성은 배지에 함유된 2,4-D 농도에 의존적이며, 1㎎/ℓ 2,4-D와 0.1㎎/ℓ kinetin을 동시에 처리하였을 때 가장 잘 형성되었다. AgNO₃를 처리한 경우 50μM의 농도에서 가장 높은 생체량 증가를 일으켰으며 갈변 억제효과와 절편체 생존율 증가를 나타내었다. BA와 NAA를 조합 처리한 재분화 배지로부터 shoot가 분화되었는데, ‘삼진’ 자엽 유래 캘러스는 5-9㎎/ℓ BA와 0.1-0.5㎎/ℓ NAA 농도로부터 shoot가 분화되었으며, ‘삼진’ 배축 유래 캘러스로부터는 shoot가 분화되지 않았다. ‘서울’ 자엽 유래 캘러스는 3-9㎎/ℓ BA와 0.1-0.5㎎/ℓ NAA 농도로부터 shoot가 분화되었으며, ‘서울’ 배축 유래 캘러스는 1-9㎎/ℓ BA와 0.1-3㎎/ℓ NAA 농도로부터 shoot가 분화되었다. 형성된 shoot는 0.1㎎/ℓ NAA를 함유하는 MS배지로 옮겨진 후 10일 이내에 뿌리가 형성되어 정상적인 식물체로 생장하였다. The effects of growth regulators and AgNO₃ on callus formation and plant regeneration have been investigated. Calli were induced from the hypocotyl and cotyledon explants of 6-day old seedlings. Calli were induced most frequently in the MS basal medium containing 1 mg/1 2,4-D and 0.1 mg/l kinetin. Callus growth rate and explant survival rate were most enhanced at 50 μM AgNO₃, and callus browning was inhibited at the presence of AgNO₃. Shoots were formed on the regeneration medium supplemented with BA combined with NAA. Shoots were formed on the medium supplemented with 5-9 mg/l BA and 0.1-0.5 mg/l NAA from the callus derived from cotyledon explants of cv. Samjin, but hypocotyl explants were not regenerated. Shoots were regenerated with 3-9 mg/l BA and 0.1-0.5 mg/1 NAA from the callus derived from cotyledon explants of cv. Seoul, and 1-9 mg/l BA and 0.1-3 mg/l NAA from the callus derived from hypocotyl explants of cv. Seoul. Shoots were transferred to the MS medium supplemented with 0.1 mg/l NAA, and roots were formed within 10 days, and grew into normal plants.
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한방병원 뇌졸중환자의 삶의 질과 자기효능감, 가족지지와의 관계 연구
김혜숙,김이순 韓國保健敎育.健康增進學會 2003 보건교육건강증진학회지 Vol.20 No.1
The purpose of this study was to analyze the relationship between quality of life, self-efficacy and family support of stroke patients in Oriental medicine hospitals. The subjects of this study were 211 hospitalized stroke patients in seven oriental medicine hospitals in the Busan Metropolitan, Gyeongsangnam-do and Gyeongsangbuk-do area. Data was collected through questionnaires from June 1st, 2002 to September 30th, 2002. 1) The relationship between general characteristics and other variables showed significant differences as follows : for general self-efficacy degree, age, occupation, monthly income, level of education, and religion ; for specific self-efficacy degree, age, occupation, and level of education ; for family support, level of education and occupation ; for quality of life, level of education, occupation, religion, and monthly income. 2) The relationship between disease-related characteristics and other variables showed significant differences as follows : for general self-efficacy, level of paralysis, and periods of hospitalization ; for specific self-efficacy, level of paralysis, and periods of hospitalization ; for family support, care-giver, character of paralysis, level of paralysis, and periods of hospitalization related; for quality of life, level of paralysis, and periods of hospitalization. 3) There was a significant correlation between the quality of life and general self-efficacy, the quality of life and the specific self- efficacy, the specific self-efficacy and family support.
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노이경;황연숙 한양대학교 2007 韓國 生活 科學 硏究 Vol.27 No.2
The objective of this study was to valuate the amenity of community facility in Complex Apartment. This study was conducted questionnaire survey. The subject of the case studies were the 6 Complex Apartments in Seoul and Gyeonggi-do built in 2000 and after, and the subject of the questionnaire was surveyed to the 212 residents who have been living in these apartments for longer than 6 months. The frequency, percentage, mean, One-way ANOVA and Duncan tests were executed through SPSS for Win 12.0 statistics package program. The types of amenity of community facility in Complex Apartments were consisted of physical aspect, spatial aspect, psychological aspect. The amenity of community facility showed high in general. Psychological aspect showed higher satisfaction of amenity level, which was followed by spatial aspect, physical aspect.
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강훈이,서인숙,이호신 麗水水産大學校 産業技術硏究所 1995 産業基術硏究所 論文集 Vol.4 No.-
This study was designed to observe the effects of feeding dietary fiber on the improvement of the fatty acid metabolism in brain, heart and lung in dietary hypercholesterolemic rats. Experimental groups with 5% level of cellulose(A group as control group), chinese cabbage root(B group),tora stalk (C group)and sweet potato stalk (D group)were adminstered to the male rats of the Sprague-Dawley for 4 weeks. The results obtaind are as follows. In the fatty acid composition of total lipid, phospholipid in the brain, the docosahexaenoic acid in the PUFA was major fatty acid. In the fatty acid composition of total lipid, phospholipid in the brain, the docosahexaenoic acid in the PUFA was major fatty acid. While in the fatty acid composition total lipid and phospholipid in the heart, the eicosapentaenoic acid in the PUFA was major fatty acid. And in the fatty acid composition of total lipid and phospholipid in the lung, the content of palmitic acid and oleic acid were higer than other fatty acid. These fats were influenced by the fatty acid composition of the test lipids. From the above result, the feeding of chinese root was effective on the improvement of the lipid compositions in principal organ tissue.
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김지현,김이선,한지숙 대한영양사협회 2004 대한영양사협회 학술지 Vol.10 No.1
The purpose of this study was to investigate disinfection state and effective factors of foodservice facilities and utilities of elementary schools in Busan area. Foodservice facilities and utilities investigated in this study were ceiling, wall, floor, trench, greasetrap, hood, pest and dumb waiter. The questionnaire which was administered to 196 dietitians was used as a survey method. The results were as follows. The washing method of foodservice facilities and utilities was mainly used by detergent washing-natural dry. The disinfection method of foodservice facilities and utilities was mainly sanitized by sodium hypochlorite. Dumb waiter and hood were sanitized by 70% ethyl alcohol and iodine. In washing and disinfection frequencies of foodservice facilities and utilities ceiling was twiceiyear, wall, hood and pest control facilities were once/week, floor, trench, greasetrap and dumb waiter were everyday, respectively. Floor, trench, greasetrap and dumb waiter were disinfected properly, whereas ceiling, wall, hood and pest were not disinfected properly. The washing method was related to dietitian's marital status and education. The disinfection state was related to dietitian's age and career, having of sanitation check list. The disinfection method was also affected dietitian' s marital status, number of employee, duration of foodservice, number of total serving and having of sanitation check list. Therefore based on the results of this study, it should be given to the microbiological study on disinfection of facilities and utilities such as ceiling, wall, floor, trench, greasetrap, hood, pest and dumb waiter and the dietitian and employee's sanitation training also should be conducted continuously.
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송계용,김숙이,서중석,지제근 중앙대학교 의과대학 의과학연구소 1991 中央醫大誌 Vol.16 No.3
To observe the developing process of human fetal eccrine sweat gland during the intrauterine life, an electron microscopic study was carried out based on 12 fetuses ranging from 20 to 42 weeks of gestation. These fetuses were the products of induced deliveries found to have not associated disease or congenital anomalies at the autopsy. Following observations were made. The various types of cell consisting coiled portion of eccrine sweat gland were recognized at the 20th weed of gestation and they were clear cells, undifferentiated dard cells, myoepithelial cells. clear cells were located in central central portion and dark or myoepithelial cells were in peripheral portion. While undifferentiated dark cells were randomly distributed in the straight portion of eccrine sweat gland. The differential points between clear cell and undifferentiated dark cell were its round nucleus and glycogen contents. Marked hydropic changes of clear cells were noted at the 30th week of gestation. Secretory granules were noted at the 31st week of gestation. Secretory cells in the coiled portion of eccrine sweat gland was not fully mature until the 42nd week of gestation. Clear cells showed clear cytoplasm, abundant free ribosomes and scanty organelles. Undifferentiated dark cells showed many mitochondria and few glycogen. Secretory granules were rarely seen. Myoepithelial cells showed a few mitochondria, glycogen and microfilaments. Among three portions of eccrine gland, duct lumen in the acral and coiled portion were formed through the intracellular canaliculus and in the straight portion were made through the intercellular canaliculus. The main basic morphological mechanism of the lumen formation were microvesicle formation and pinched off phenomenon.
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Sook Yi Song,Sunggil Kim,Haejeen Bang,Cheol-Woo Kim,Jin Seong Moon,Bhimanagouda S. Patil 한국육종학회 2013 한국육종학회 심포지엄 Vol.2013 No.07
Inactivation of the gene (DFR-A) coding for dihydroflavonol 4-reductase (DFR) involved in the anthocyanin biosynthesis pathway results in a yellow bulb color in onion (Allium cepa L.) and three inactive alleles have previously been identified in onion. Additionally, three active and six inactive DFR-A alleles were newly identified from extensive analyses of diverse onion germplasm. Presently, a yellow mutant containing a 171-bp deletion in the promoter region was identified and designated DFR-APD. Critically reduced transcription of this mutant allele and perfect co-segregation with color phenotypes in segregating populations were observed. Another yellow mutant (DFR-A5’DEL) containing a 518-bp deletion covering exons 1 and 2, which played important roles in DFR function, was identified. Meanwhile, both 2-bp and 4-bp insertions in the coding region leading to creation of pre-mature stop codons were also identified and designated DFR-AGT and DFR-A2AT, respectively. A 1-bp substitution mutation (DFR-AK48N) changing a positively charged lysine residue into a neutral asparagine was identified. This lysine residue, a NADPH binding site, was strictly conserved in other species. In addition, insertion of a leucine residue around substrate binding sites and catalytic triad was identified in several yellow accessions and was designated DFR-ATTA. Phylogenetic analysis of DFR-A alleles showed that all inactive alleles were independently derived from four different active alleles. In addition, the close relatedness and diversity of DFR-A mutants implied that all these mutations might have occurred after domestication of onions and had probably been maintained by artificial selection.
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Yi, Young-Joo,Park, Chang-Sik,Kim, Eui-Sook,Song, Eun-Sook,Jeong, Ji-Hyeon,Sutovsky, Peter Informa Healthcare 2009 Systems biology in reproductive medicine Vol.55 No.2
<P>Extracellular ATP has been implicated in a number of cellular events, including mammalian sperm function. The complement of ATP-dependent sperm proteins includes six subunits of the 26S proteasome, a multi-subunit protease specific to ubiquitinated substrate-proteins. Proteolysis of ubiquitinated proteins by the 26S proteasome is necessary for the success of mammalian fertilization, including but not limited to acrosomal exocytosis (AE) and sperm-zona pellucida (ZP) penetration. The 26S proteasome is uniquely present on the sperm acrosomal surface during mammalian, ascidian, and invertebrate fertilization. The proteasome is a multi-subunit protease complex of approximately 2 MDa composed of the 19S regulatory complex and a 20S proteolytic core. Integrity of the 19S complex is maintained by six 19S ATPase subunits (PSMC1 through PSMC6). Consequently, we hypothesized that fertilization will be blocked by the depletion of sperm-surface associated ATP (ssATP). Depletion of ssATP by the Solanum tuberosum apyrase, a 49 kDa, non-cell permeant enzyme, significantly reduced the ATP content measured by an adapted luminescence-ATP assay from which all permeabilizing agents were excluded. Addition of active apyrase to porcine in vitro fertilization (IVF) medium caused a concentration dependent reduction in the overall fertilization rate. No such outcomes were observed in control groups using heat-inactivated apyrase. Apyrase treatment altered the band pattern of 19S ATPase subunits PSMC1 (Rpt2) and PSMC4 (Rpt3) in Western blotting, suggesting that it had an effect on the integrity of the sperm proteasomal 19S complex. Apyrase only altered the proteasomal core activities slightly, since these activities are not directly dependent on external ATP. In contrast, sperm treatment with MG132, a specific inhibitor of the proteasomal core chymotrypsin-like activity, inhibited the target proteolytic activity, but also induced a compensatory elevation in proteasomal peptidyl-glutamyl peptide hydrolase activity. Altogether, the present data provide an important missing piece of evidence in support of the ssATP-dependent, proteasomal-proteolytic model of sperm-ZP interactions.</P>
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