Synergistic effect of binanoenzyme and cryogel column on the production of formic acid from carbondioxide

Yi gitcan Sümbelli,Özlem Biçen Ünlüer,Arzu Ersöz,Rıdvan Say 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.76 No.-

In this study, binanoenzyme (BNE) was synthesized using FateDH and Cyt-C in same bionano-interface;by formation of formate dehydrogenase-co-cytochrome C by using ANADOLUCA method. Thesynthesized novel binanoenzyme was embedded into HEMA based cryogel column and the conversionof CO2 to formic acid was investigated by using this cryogel column. To maintain the conversion, CO2saturated water was passed through the cryogel column and the converted formic acid was analyzedusing HPLC. The parameters which has an effect on conversion such as pH, sample volume and CO2concentration were analyzed. The results showed that the conversion process was succeeded effectively.

Comparison of the Effects of Electroacupuncture and Melatonin on Nerve Regeneration in Experimentally Nerve-Damaged Rats

Yasemin Özkan,Mehmet Turgut,Yasemin Turan,Mehmet Dinçer Bilgin,Sinem Sari,Mustafa Yilmaz,Yiğit Uyanikgil,Mahmut Alp Kiliç,Derya Tanriöver,Zehra Seznur Kasar 사단법인약침학회 2021 Journal of Acupuncture & Meridian Studies Vol.14 No.5

Background: Development of methods to accelerate nerve regeneration in peripheral nerve damage is important. Electroacupuncture is a new therapeutic method that combines traditional acupuncture with modern electrotherapy. Melatonin has been shown to reduce nerve damage. Objectives: In this study, we aimed to determine and compare the therapeutic effects of electroacupuncture and melatonin on rat sciatic nerve injury. Methods: A total of 56 adult male Wistar Albino rats were divided into four study groups with 14 animals in each group: intact control (group I), subcutaneous saline (group II), subcutaneous melatonin (group III), and electroacupuncture (group IV). Surgical procedure including unilateral (right) sciatic nerve injury was applied to groups II, III, and IV. Saline and melatonin started immediately after surgery for six weeks, while electroacupuncture was given two weeks after surgery for 3 weeks. Functional and histological assessments were used as outcome measurements. Results: Sciatic nerve damage caused a significant decrease in nerve conduction velocity. Both electroacupuncture treatment and melatonin treatment significantly increased the nerve conduction velocity. Both sciatic functional recovery and histological regeneration were faster in these treatment groups compared to the saline. However, no significant difference was observed between the two treatment groups. Conclusion: Electroacupuncture and melatonin are promising alternative treatment strategies for peripheral nerve damage and can be examined in detail in future studies.

Innovative displacement-based beam-column element with shear deformation and imperfection

Yi-Qun Tang,Yue-Yang Ding,Yao-Peng Liu,Siu-Lai Chan,Er-Feng Du 국제구조공학회 2022 Steel and Composite Structures, An International J Vol.42 No.1

The pointwise equilibrium polynomial (PEP) element considering local second-order effect has been widely used in direct analysis of many practical engineering structures. However, it was derived according to Euler-Bernoulli beam theory and therefore it cannot consider shear deformation, which may lead to inaccurate prediction for deep beams. In this paper, a novel beam-column element based on Timoshenko beam theory is proposed to overcome the drawback of PEP element. A fifth-order polynomial is adopted for the lateral deflection of the proposed element, while a quadric shear strain field based on equilibrium equation is assumed for transverse shear deformation. Further, an additional quadric function is adopted in this new element to account for member initial geometrical imperfection. In conjunction with a reliable and effective three-dimensional (3D) corotational technique, the proposed element can consider both member initial imperfection and transverse shear deformation for second-order direct analysis of frame structures. Some benchmark problems are provided to demonstrate the accuracy and high performance of the proposed element. The significant adverse influence on structural behaviors due to shear deformation and initial imperfection is also discussed.

Preoperative BRAF Mutation is Predictive of Occult Contralateral Carcinoma in Patients with Unilateral Papillary Thyroid Microcarcinoma

Zhou, Yi-Li,Zhang, Wei,Gao, Er-Li,Dai, Xuan-Xuan,Yang, Han,Zhang, Xiao-Hua,Wang, Ou-Chen Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.4

Background and Objective: The optimal resection extent for clinically unilateral papillary thyroid microcarcinoma (PTMC) remains controversial. The objective was to investigate risk factors associated with occult contralateral carcinoma, and put emphasis on the predictive value of preoperative BRAF mutation. Materials and Methods: 100 clinically unilateral PTMC patients all newly diagnosed, previously untreated were analyzed in a prospective cohort study. We assessed the T1799A BRAF mutation status in FNAB specimens obtained from all PTMC patients before undergoing total thyroidectomy (TT) and central lymph node dissection (CLND) for PTMC. Univariate and multivariate analyses were used to reveal the incidence of contralateral occult cancer, difference of risk factors and predictive value, with respect to the following variables: preoperative BRAF mutation status, age, gender, tumor size, multifocality of primary tumor, capsular invasion, presence of Hashimoto thyroiditis and central lymph node metastasis. Results: 20 of 100 patients (20%) had occult contralateral lobe carcinoma. On multi-variate analysis, preoperative BRAF mutation (p = 0.030, OR = 3.439) and multifocality of the primary tumor (p = 0.004, OR = 9.570) were independent predictive factors for occult contralateral PTMC presence. However, there were no significant differences between the presence of occult contralateral carcinomas and age, gender, tumor size, capsular invasion, Hashimoto thyroiditis and central lymph node metastasis. Conclusions: Total thyroidectomy, including the contralateral lobe, should be considered for the treatment of unilateral PTMC if preoperative BRAF mutation is positive and/or if the observed lesion presents as a multifocal tumor in the unilateral lobe.

Artificial Intelligence in the Prediction of Gastrointestinal Stromal Tumors on Endoscopic Ultrasonography Images: Development, Validation and Comparison with Endosonographers

Lu Yi,Wu Jiachuan,Hu Minhui,Zhong Qinghua,Er Limian,Shi Huihui,Cheng Weihui,Chen Ke,Liu Yuan,Qiu Bingfeng,Xu Qiancheng,Lai Guangshun,Wang Yufeng,Luo Yuxuan,Mu Jinbao,Zhang Wenjie,Zhi Min,Sun Jiachen 거트앤리버 소화기연관학회협의회 2023 Gut and Liver Vol.17 No.6

Background/Aims: The accuracy of endosonographers in diagnosing gastric subepithelial lesions (SELs) using endoscopic ultrasonography (EUS) is influenced by experience and subjectivity. Artificial intelligence (AI) has achieved remarkable development in this field. This study aimed to develop an AI-based EUS diagnostic model for the diagnosis of SELs, and evaluated its efficacy with external validation. Methods: We developed the EUS-AI model with ResNeSt50 using EUS images from two hospitals to predict the histopathology of the gastric SELs originating from muscularis propria. The diagnostic performance of the model was also validated using EUS images obtained from four other hospitals. Results: A total of 2,057 images from 367 patients (375 SELs) were chosen to build the models, and 914 images from 106 patients (108 SELs) were chosen for external validation. The sensitivity, specificity, positive predictive value, negative predictive value, and accuracy of the model for differentiating gastrointestinal stromal tumors (GISTs) and non-GISTs in the external validation sets by images were 82.01%, 68.22%, 86.77%, 59.86%, and 78.12%, respectively. The sensitivity, specificity, positive predictive value, negative predictive value, and accuracy in the external validation set by tumors were 83.75%, 71.43%, 89.33%, 60.61%, and 80.56%, respectively. The EUS-AI model showed better performance (especially specificity) than some endosonographers. The model helped improve the sensitivity, specificity, and accuracy of certain endosonographers. Conclusions: We developed an EUS-AI model to classify gastric SELs originating from muscularis propria into GISTs and non-GISTs with good accuracy. The model may help improve the diagnostic performance of endosonographers. Further work is required to develop a multi-modal EUS-AI system.

Prognostic Significance of 18F-fluorodeoxyglucose Positron Emission Tomography (PET)-based Parameters in Neoadjuvant Chemoradiation Treatment of Esophageal Carcinoma

Ma, Jin-Bo,Chen, Er-Cheng,Song, Yi-Peng,Liu, Peng,Jiang, Wei,Li, Ming-Huan,Yu, Jin-Ming Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.4

Aims and Background: The purpose of the research was to study the prognostic value of tumor 18F-FDG PET-based parameters in neoadjuvant chemoradiation for patients with squamous esophageal carcinoma. Methods: Sixty patients received chemoradiation therapy followed by esophagectomy and two 18FDG-PET examinations at pre- and post-radiation therapy. PET-based metabolic-response parameters were calculated based on histopathologic response. Linear regression correlation and Cox proportional hazards models were used to determine prognostic value of all PET-based parameters with reference to overall survival. Results: Sensitivity (88.2%) and specificity (86.5%) of a percentage decrease of SUVmax were better than other PET-based parameters for prediction of histopathologic response. Only percentage decrease of SUVmax and tumor length correlated with overall survival time (linear regression coefficient ${\beta}$: 0.704 and 0.684, P<0.05). The Cox proportional hazards model indicated higher hazard ratio (HR=0.897, P=0.002) with decrease of SUVmax compared with decrease of tumor size (HR=0.813, P=0.009). Conclusion: Decrease of SUVmax and tumor size are significant prognostic factors in chemoradiation of esophageal carcinoma.

The evaluation of online course of Traditional Chinese Medicine for Medical Bachelor, Bachelor of Surgery international students during the COVID-19 epidemic period

Zhang Qing,He Yi-Jing,Zhu Yu-Hang,Dai Min-Chen,Pan Man-Man,Wu Jia-Qi,Zhang Xian,Gu Ying-Er,Wang Fang-Fang,Xu Xiang-Rong,Qu Fan 한국한의학연구원 2020 Integrative Medicine Research Vol.9 No.3

Background: During the COVID-19 epidemic period, Traditional Chinese Medicine (TCM) course for international students of Medical Bachelor, Bachelor of Surgery (MBBS) program in Zhejiang University has shifted from traditional classroom to online environment. This study aimed to investigate MBBS international students’ perception on online TCM course, and to assess the online learning efficacy. Methods: A total of 84 MBBS international students attending course of “Basic Traditional Chinese Medicine” during 2020 academic years at Zhejiang University were enrolled in this study. A quantitative questionnaire was respectively completed before and after the TCM course using a pretest–post-test design. By means of two online learning platforms, Learning in ZJU and DingTalk, TCM course was broadcast in both live and archived format to students. Results: A total of 48 participants completed both baseline and follow-up questionnaires. The majority of participants preferred face-to-face classroom learning (26, 54.17% of total) when compared with online learning. Students felt that the course had brought in much benefits (mean 3.88, SD 0.87), and they were satisfied with the course content (mean 3.83, SD 0.95). Students’ TCM related knowledge and their behaviors of discussion and consulting were significantly improved by online TCM course (all P < 0.001). Students’ awareness of the necessity of TCM education and their feeling of difficulty in learning TCM were significantly strengthened (P = 0.042, 0.025, respectively). Conclusion: Online learning is a good alternative for TCM course of MBBS international students when classroom learning is suspended, whereas it cannot replace the need for onsite and face-to-face learning.

Moderately Hypofractionated Conformal Radiation Treatment of Thoracic Esophageal Carcinoma

Ma, Jin-Bo,Wei, Lin,Chen, Er-Cheng,Qin, Guang,Song, Yi-Peng,Chen, Xiang-Ming,Hao, Chuan-Guo Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.8

Aims: To prospectively assess the efficacy and safety of moderately hypofractionated conformal radiotherapy in patients with thoracic esophageal cancer. Methods and Materials: From Sept. 2002 to Oct, 2005, 150 eligible patients with T2-4N0-1M0 stage thoracic esophageal squamous cell cancers were enrolled to receive either conventional fractionated radiation (CFR) or moderately hypofractionated radiation (MHR) with a three-dimensional conformal radiation technique. Of the total, 74 received moderately hypofractionated radiation with total dose of 54-60Gy/18-20fractions for 3.5-4 weeks in the MHR arm, and 76 received conventional radiation with total dose of 60Gy/30 fractions for 6 weeks in the CFR arm. Concurrent chemotherapy comprised of paclitaxel and cisplatin. Safety was evaluated, and local control and overall survival rates were calculated. Results: Statistically significant differences between the CFR versus MHR arms were observed in local/regional failure rate (47.3% v 27.0%, P=0.034) and the percentage of patients with persistent local disease (26.3% v 10.8%, P=0.012). But 3 and 5-year overall survival rates (43.2%, 38.8% v 38.2%, 28.0%, respectively) were not different between the two arms (P=0.268). There were no significant differences in the incidences of grade 3 or higher acute toxicities (66.3% v 50.0%) and late complications rates (27.0% v 22.4%) between the MHR and CFR arms. Conclusions: Moderately hypofractionated, three-dimensional radiation treatment could improve the local control rate of esophageal cancer and potentially increase patients' survival.

Efficient DFT/DCT Computation for OFDM in Cognitive Radio System

진주,김정기,얀이얼,이문호,Chen, Zhu,Kim, Jeong-Ki,Yan, Yi-Er,Lee, Moon-Ho The Institute of Electronics and Information Engin 2008 電子工學會論文誌-TC (Telecommunications) Vol.45 No.2

본 논문에서는 Cognitive Radio 시스템에서의 DFT와 DCT에 근거한 OFDM을 제시한다. 적응 OFDM은 허가된 사용자의 주파수 간섭을 피하기위해서 개별적인 반송파를 무효화시키는 capacity를 갖는다. 그러므로 OFDM 송신기에서 IDFT/DFT, IDCT/DCT의 입력과 출력이 상당수의 0값을 갖는다. 따라서 DFT와 DCT의 표준 방법은 0에서 필요치 않은 연산 때문에 더 이상 효율적이지 않을 수 있다. 이러한 고찰에 근거하여, 본 논문은 IDFT/DFT, IDCT/DCT를 위한 2차원적(2-D) 단축된 정렬 변환 분해 방법을 보이고, 이 알고리즘이 Cognitive Radion 시스템 환경의 OFDM의 계산을 효율적으로 수행 할 수 있는 방법을 제시한다. In this paper, we address the OFDM based on DFT or DCT in Cognitive Radio system. An adaptive OFDM based on DFT or DCT in Cognitive Radio system has the capacity to nullify individual carriers to avoid interference to the licensed users. Therefore, there could be a considerably large number of zero-valued inputs/outputs for the IDFT/DFT or IDCT/DCT on the OFDM transceiver. Hence, the standard methods of DFT and DCT are no longer efficient due to the wasted operations on zero. Based on this observation, we present a transform decomposition on two dimensional(2-D) systolic array for IDFT/DFT and IDCT/DCT, this algorithm can achieve an efficient computation for OFDM in Cognitive Radio system

Simultaneous Blockage of Epidermal Growth Factor Receptor and Cyclooxygenase-2 in a Human Xenotransplanted Lung Cancer Model

Mu, Xiao-Yan,Dong, Xue-Li,Sun, Jie,Ni, Yu-Hua,Dong, Zhang,Li, Xi-Li,Sun, Er-Lian,Yi, Zhou,Li, Gao Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.1

The effects of erlotinib combined with celecoxib in a lung cancer xenograft model were here explored with a focus on possible mechanisms. A xenotransplanted lung cancer model was established in nude mice using the human lung cancer cell A549 cell line and animals demonstrating tumour growth were randomly divided into four groups: control, erlotinib, celecoxib and combined (erotinib and celecoxib). The tumor major axis and short diameter were measured twice a week and after 40 days tissues were collected for immunohistochemical analyses of Bcl-2 and Bax positive cells and Western-blotting analyses for the epidermal growth factor recepto (EGFR), P-EGFR, and cyclooxygenase-2 (COX-2). Tumor size in the combined group was smaller than in the others (p<0.01) and the percentage of Bcl-2 positive cells was fewer in most cases (p<0.01), while that of Bax positive cells was greater than in the erlotinib and celecoxib groups (P>0.05). Western blotting showed decreased expression of P-EGFR and COX-2 with both erlotinib and celecoxib treatments, but most pronouncedly in the combined group (P<0.05). Simultaneous blockage of the EGFR and COX-2 signal pathways exerted stronger growth effects in our human xenotransplanted lung cancer model than inhibition of either pathway alone. The anti-tumor effects were accompanied by synergetic inhibition of tumor cell apoptosis, activation of p-EGFR and expression of COX-2.