PMCN: Combining PDF-modified Similarity and Complex Network in Multi-document Summarization

Yi-Ning Tu,Wei-Tse Hsu 건국대학교 지식콘텐츠연구소 2019 International Journal of Knowledge Content Develop Vol.9 No.3

Hypolipidemic and Antioxidative Effects of Glossogyne tenuifolia in Hamsters Fed an Atherogenic Diet

Yi-Ning Lee,Guoo-Shyng Wang Hsu,Wan-Teng Lin,Yi-Fa Lu 한국식품영양과학회 2016 Journal of medicinal food Vol.19 No.5

Glossogyne tenuifolia (GT) Cassini is a special herbal tea in the Penghu Islands, Taiwan, and has a long history of being used as an antipyretic, detoxifying, and anti-inflammatory remedy in folk medicine among local residents. The aim of this study was to investigate the effect of hot water extracts from GT on oxidative stress and lipid metabolism in animals. Five- to 6-week-old male Syrian hamsters were divided into four groups (n = 14) for different treatments, that is: control group (C), high-fat/cholesterol (HF) group, HF diet containing 0.5% (GT0.5) and 1.5% (GT1.5) GT extracts for 4 weeks. Hamsters fed with 0.5% GT powder as well as 1.5% GT powder exhibited reduced serum total cholesterol (TC), conjugated diene of low-density lipoprotein (LDL), and increased serum antioxidant capacity, but 1.5% GT powder was more potent at loweringserum LDL cholesterol and thiobarbituric acid reactive substance concentrations than 0.5% GT. GT extracts significantly lowered liver triacylglycerol (TG) concentration by diminishing activities of fatty acid synthase (FAS) and glucose-6-phosphate dehydrogenase (G-6-PDH). In addition, fecal excretion of cholesterol and bile acids were increased in GT extract groups. In conclusion, GT extracts increase the antioxidative capacity, decrease serum TC, inhibit the activities of FAS and G-6-PDH, and further reduce liver TG accumulation in hamster fed on atherogenic diets.

Identifying genes for resistant starch, slowly digestible starch, and rapidly digestible starch in rice using genome-wide association studies

Ning Zhang,Maike Wang,Ji Fu,Yi Shen,Yi Ding,Dianxing Wu,Xiaoli Shu,Wenjian Song 한국유전학회 2020 Genes & Genomics Vol.42 No.11

Background The digestibility of starch is important for the nutritive value of staple food. Although several genes are responsible for resistant starch (RS) and slowly digestible starch (SDS), gaps persist concerning the molecular basis of RS and SDS formation due to the complex genetic mechanisms of starch digestibility. Objectives The objective of this study was to identify new genes for starch digestibility in rice and interprete the genetic mechanisms of RS and SDS by GWAS. Methods Genome-wide association studies were conducted by associating the RS and SDS phenotypes of 104 re-sequenced rice lines to an SNP dataset of 2,288,867 sites using a compressed mixed linear model. Candidate genes were identifed according to the position of the SNPs based on data from the MSU Rice Genome Annotation Project. Results Seven quantitative trait loci (QTLs) were detected to be associated with the RS content, among which the SNP 6 m1765761 was located on Waxy. Starch branching enzymes IIa (BEIIa) close to QTL qRS-I4 was detected and further identifed as a specifc candidate gene for RS in INDICA. Two QTLs were associated with SDS, and the LOC_Os09g09360 encoding lipase was identifed as a causal gene for SDS. Conclusions GWAS is a valid strategy to genetically dissect the formation of starch digestion properties in rice. RS formation in grains is dependent on the rice type; lipid might also contribute to starch digestibility and should be an alternative factor to improve rice starch digestibility.

Design of a Non-destructive Fluorescent-image-based Fresh Meat Contamination Inspection System

( Yi-chich Chiu ),( Yi-ning Ho ) 한국농업기계학회 2018 한국농업기계학회 학술발표논문집 Vol.23 No.1

Health and safety of meat can be public concerns.Moreover, sanitation monitoring of a processing plant or a processing line is directly related to food safety.This research aims to develop a non-destructive contamination inspection system for livestock fresh food products. Fluorescent image analyzing techniques is used to detect and identify food product contamination of Escherichia coli.Fluorescence spectra data of these food product contamination is established to provide early detection of food safety problems to reduce food safety incidents.The potential of fluorescence spectroscopy was investigated for the non-destructive evaluation of Escherichia coli content and plate count on pork meat surface stored aerobically at 14 °C during. Use of excitation light and emission wavelength 200nm~400nm Excitation (Ex) Emission (Em) Matrix of fluorescence intensity was obtained and fluorescence from Escherichia coli (Ex=295 nm and Em=330 nm) was detected. Microbial spoilage on meat could be detected from fluorescence. On pork meat surface, Escherichia coli content and plate count showed high correlation. Therefore of Escherichia coli content could be a parameter of sanitation monitoring.In the Excitation Emission Matrix of fluorescence intensity, the fluorescence of of Escherichia coli was detected. Main tasks of this study include developing systems and technologies to inspect and etermine whether pork were contaminated by E. coli. Fluorescence detection and analysis systems, is design and developed based on LabVIEW graphical programing language.

Mechanistic Analysis of Taxol-induced Multidrug Resistance in an Ovarian Cancer Cell Line

Wang, Ning-Ning,Zhao, Li-Jun,Wu, Li-Nan,He, Ming-Feng,Qu, Jun-Wei,Zhao, Yi-Bing,Zhao, Wan-Zhou,Li, Jie-Shou,Wang, Jin-Hua Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.9

Objectives: To establish a taxol-resistant cell line of human ovarian carcinoma (A2780/Taxol) and investigate its biological features. Methods: The drug-resistant cell line (A2780/Taxol) was established by continuous stepwise selection with increasing concentrations of Taxol. Cell morphology was assessed by microscopy and growth curves were generated with in vitro and in vivo tumor xenograft models. With rhodamine123 (Rh123) assays, cell cycle distribution and the apoptotic rate were analyzed by flow cytometry (FCM). Drug resistance-related and signal associated proteins, including P-gp, MRPs, caveolin-1, PKC-${\alpha}$, Akt, ERK1/2, were detected by Western blotting. Results: A2780/Taxol cells were established with stable resistance to taxol. The drug resistance index (RI) was 430.7. Cross-resistance to other drugs was also shown, but there was no significant change to radioresistance. Compared with parental cells, A2780/Taxol cells were significantly heteromorphous, with a significant delay in population doubling time and reduced uptake of Rh123 (p<0.01). In vivo, tumor take by A2780 cells was 80%, and tumor volume increased gradually. In contrast, with A2780/Taxol cells in xenograft models there was no tumor development. FCM analysis revealed that A2780/Taxol cells had a higher percentage of G0/G1 and lower S phase, but no changes of G2 phase and the apoptosis rate. Expression of P-gp, MRP1, MRP2, BCRP, LRP, caveolin-1, PKC-${\alpha}$, Phospho-ERK1/2 and Phospho-JNK protein was significantly up-regulated, while Akt and p38 MARK protein expression was not changed in A2780/Taxol cells. Conclusion: The A2780/Taxol cell line is an ideal model to investigate the mechanism of muti-drug resistance related to overexpression of drug-resistance associated proteins and activation of the PKC-${\alpha}/ERK$ (JNK) signaling pathway.

Clinical Prognostic Factors and Survival Outcome in Renal Cell Carcinoma Patients - A Malaysian Single Centre Perspective

Yap, Ning Yi,Ng, Keng Lim,Ong, Teng Aik,Pailoor, Jayalakshmi,Gobe, Glenda Carolyn,Ooi, Chong Chien,Razack, Azed Hassan,Dublin, Norman,Morais, Christudas,Rajandram, Retnagowri Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.12

Background: This study concerns clinical characteristics and survival of renal cell carcinoma (RCC) patients in University Malaya Medical Centre (UMMC), as well as the prognostic significance of presenting symptoms. Materials and Methods: The clinical characteristics, presenting symptoms and survival of RCC patients (n=151) treated at UMMC from 2003-2012 were analysed. Symptoms evaluated were macrohaematuria, flank pain, palpable abdominal mass, fever, lethargy, loss of weight, anaemia, elevated ALP, hypoalbuminemia and thrombocytosis. Univariate and multivariate Cox regression analyses were performed to determine the prognostic significance of these presenting symptoms. Kaplan Meier and log rank tests were employed for survival analysis. Results: The 2002 TNM staging was a prognostic factor (p<0.001) but Fuhrman grading was not significantly correlated with survival (p=0.088). At presentation, 76.8% of the patients were symptomatic. Generally, symptomatic tumours had a worse survival prognosis compared to asymptomatic cases (p=0.009; HR 4.74). All symptoms significantly affect disease specific survival except frank haematuria and loin pain on univariate Cox regression analysis. On multivariate analysis adjusted for stage, only clinically palpable abdominal mass remained statistically significant (p=0.027). The mean tumour size of palpable abdominal masses, $9.5{\pm}4.3cm$, was larger than non palpable masses, $5.3{\pm}2.7cm$ (p<0.001). Conclusions: This is the first report which includes survival information of RCC patients from Malaysia. Here the TNM stage and a palpable abdominal mass were independent predictors for survival. Further investigations using a multicentre cohort to analyse mortality and survival rates may aid in improving management of these patients.

미국·일본 애니메이션 영화 소비의도 결정요인에 대한 탐색적 연구 : 한국과 중국 대학생들의 비교

녕정이(Ging-yi Ning),전범수(Bum-Soo Chon) 한국애니메이션학회 2020 애니메이션연구 Vol.16 No.1

이 연구는 같은 아시아 국가인 한국과 중국의 대학생들이 미국 및 일본의 애니메이션 영화를 왜 관람하고 소비하게 되는지 그 차이를 살펴보기 위한 것이다. 구체적으로는 한국과 중국 대학생들을 대상으로 미국과 일본 애니메이션 영화의 관람 동기를 포함해 애니메이션 스튜디오 대한 선호도, 그리고 미국과 일본에 대한 태도가 실질적으로 애니메이션 영화 소비의도에 영향을 미치는가를 분석했다. 이 연구의 주요 분석 결과는 다음과 같다. 첫째, 한국 대학생의 미국 애니메이션 소비의도에 대한 결정 요인을 분석하기 위해 회귀 분석을 실시한 결과, ‘미국 문화에 대한 태도’가 긍정적일수록, 그리고 ‘즐거움’ 동기가 강할수록 한국 대학생의 미국 애니메이션 영화 소비 의도는 증가했다. 회귀 모델 설명력은 67%로 나타났다. 한편, 중국 대학생의 경우에는 ‘유용성 동기’가 강하거나 ‘미국 애니메이션 스튜디오 선호도’가 높을 때 중국 대학생들의 미국 애니메이션 영화 소비 의도가 증가했다. 회귀 모델 설명력은 75%로 나타났다. 둘째, 한국 대학생의 일본 애니메이션 소비의도에 대한 결정 요인을 분석하기 위해 회귀 분석을 실시한 결과, 일본 문화에 대한 태도가 긍정적일수록, 일본 애니메이션 스튜디오 브랜드를 선호할수록, 즐거움 동기가 약할수록 일본 애니메이션 영화 소비의도가 증가하는 것으로 나타났다. 회귀 모델의 설명력은 67%로 나타났다. 중국 대학생들의 경우에 일본 애니메이션 스튜디오 브랜드 선호도가 높을수록, 일본 문화 관심 동기가 높을수록 일본 애니메이션 영화 소비의도가 증가하는 것으로 나타났다. 회귀 모델의 설명력은 72%로 나타났다. This study aims to examine determinants of the intention to watch both US and Japanese animation movies between Korean and Chinese college students. In this study, the key defendant variable is the intention to watch animation movies, and the independent variables were comprised of three factors including motives to watch animation movies, preferences for US and Japanese animation studios and attitudes toward the US and Japan. The major results are as follows; firstly, for Korean college students, attitude toward US culture and motives of entertainment and experience were significant predictors in explaining the intention to watch US animation movies. This regression model explained 67% of the variance. For Chinese college students, preferences for US animation studios and motives of usefulness were significant predictors in explaining the intention to watch Japanese animation movies. This regression model explained 75% of the variance. Secondly, for Korean college students, attitude toward Japanese culture, preferences for Japanese animation studios were significant predictors in explaining the intention to watch Japanese animation movies, while motives of usefulness was the negative factor. This regression model explained 67% of the variance. For Chinese college students, preferences for Japanese animation studios and attitude toward Japanese culture were significant predictors in explaining the intention to watch Japanese animation movies. This regression model explained 72% of the variance.

Preclinical Study of Cell Therapy for Osteonecrosis of the Femoral Head with Allogenic Peripheral Blood-Derived Mesenchymal Stem Cells

Tao Zhang,Qiang Fu,Ning-Ning Tang,Qian Zhang,Yi Liu,Jia-Chen Peng,Ning Fang,Li-Mei Yu,Jin-Wei Liu 연세대학교의과대학 2016 Yonsei medical journal Vol.57 No.4

Purpose: To explore the value of transplanting peripheral blood-derived mesenchymal stem cells from allogenic rabbits (rPBMSCs)to treat osteonecrosis of the femoral head (ONFH). Materials and Methods: rPBMSCs were separated/cultured from peripheral blood after granulocyte colony-stimulating factor mobilization. Afterwards, mobilized rPBMSCs from a second passage labeled with PKH26 were transplanted into rabbit ONFH models, which were established by liquid nitrogen freezing, to observe the effect of rPBMSCs on ONFH repair. Then, the mRNA expressions of BMP-2 and PPAR-γ in the femoral head were assessed by RT-PCR. Results: After mobilization, the cultured rPBMSCs expressed mesenchymal markers of CD90, CD44, CD29, and CD105, but failed to express CD45, CD14, and CD34. The colony forming efficiency of mobilized rPBMSCs ranged from 2.8 to 10.8 per million peripheralmononuclear cells. After local transplantation, survival of the engrafted cells reached at least 8 weeks. Therein, BMP-2 was up-regulated, while PPAR-γ mRNA was down-regulated. Additionally, bone density and bone trabeculae tended to increase gradually. Conclusion: We confirmed that local transplantation of rPBMSCs benefits ONFH treatment and that the beneficial effects are relatedto the up-regulation of BMP-2 expression and the down-regulation of PPAR-γ expression.

An update of preimplantation genetic diagnosis in gene diseases, chromosomal translocation, and aneuploidy screening

Chang, Li-Jung,Chen, Shee-Uan,Tsai, Yi-Yi,Hung, Chia-Cheng,Fang, Mei-Ya,Su, Yi-Ning,Yang, Yu-Shih The Korean Society for Reproductive Medicine 2011 Clinical and Experimental Reproductive Medicine Vol.38 No.3

Preimplantation genetic diagnosis (PGD) is gradually widely used in prevention of gene diseases and chromosomal abnormalities. Much improvement has been achieved in biopsy technique and molecular diagnosis. Blastocyst biopsy can increase diagnostic accuracy and reduce allele dropout. It is cost-effective and currently plays an important role. Whole genome amplification permits subsequent individual detection of multiple gene loci and screening all 23 pairs of chromosomes. For PGD of chromosomal translocation, fluorescence $in-situ$ hybridization (FISH) is traditionally used, but with technical difficulty. Array comparative genomic hybridization (CGH) can detect translocation and 23 pairs of chromosomes that may replace FISH. Single nucleotide polymorphisms array with haplotyping can further distinguish between normal chromosomes and balanced translocation. PGD may shorten time to conceive and reduce miscarriage for patients with chromosomal translocation. PGD has a potential value for mitochondrial diseases. Preimplantation genetic haplotyping has been applied for unknown mutation sites of single gene disease. Preimplantation genetic screening (PGS) using limited FISH probes in the cleavage-stage embryo did not increase live birth rates for patients with advanced maternal age, unexplained recurrent abortions, and repeated implantation failure. Polar body and blastocyst biopsy may circumvent the problem of mosaicism. PGS using blastocyst biopsy and array CGH is encouraging and merit further studies. Cryopreservation of biopsied blastocysts instead of fresh transfer permits sufficient time for transportation and genetic analysis. Cryopreservation of embryos may avoid ovarian hyperstimulation syndrome and possible suboptimal endometrium.

Chromatic Characteristics and Anthocyanin Compositions of Cabernet Sauvignon Wines: Influence of Indigenous Saccharomyces cerevisiae Strains in Ningxia, China

Ning Liu,Yu-Yang Song,Yi Qin,Xue Gong,Yan-Lin Liu 한국식품과학회 2015 Food Science and Biotechnology Vol.24 No.6

Eight Saccharomyces cerevisiae strains were used for preparation of Cabernet Sauvignon musts from Yuma vineyard in China, where the strains were isolated, to study the importance of yeast strain for optimization of Cabernet Sauvignon wine color and anthocyanin composition. Differences in chromatic characteristics between indigenous yeast strains and a commercial F15 control were not found. Twenty-one anthocyanins were detected and quantified using HPLC-MS. Concentrations of anthocyanins were significantly (p<0.05) influenced by the yeast strain used. Use of the commercial F15 yeast resulted in the highest anthocyanin concentration. Among 9 pyranoanthocyanins detected, amounts of must were influenced by the yeast strain used. The color of wine produced using the N11424 yeast was more stable because of a higher pyranoanthocyanin concentration. Use of Principal Component Analysis indicated that indigenous yeast treatments were separated from the F15 control yeast treatment based on anthocyanin components.