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Vinblastine이 백서 배양 Type-1 별아교세포에 미치는 세포독성에 관한 연구
하상호,박승택,문연자,김종영,김정중,정연태 圓光大學校 醫科學硏究所 1994 圓光醫科學 Vol.10 No.1-2
It has been reported that vinblastine, anticancer drug, is a neuroteratogen inducing various neural malformations such as microcephaly and neural tube defect(NTD), but the study on the toxicity of neuroglial cells, especially type-1 astrocytes using culture system is not well established. In order to evaluate the cytotoxicity of vinblastine on cultured type-1 astrocytes of neonatal rat brain in vitro. MTT assay and also light and electron microscopic studies were carried out. The results were as follows: 1. MTT_90 and MTT_50 in cultured type-1 astrocytes were 1×10 exp (-1) μM and 1×10 exp (2)μM of vinblastine, respectively. 2. Vinblastine was highly toxic in cultured type-1 astrocytes(MTT_50 ≤ 100 μM). 3. In a light microscopy, cultured type-1 astrocytes showed decrease of cells in number, cytoplasmic perforation, vacuolization and cytoplasmic granulation after cultured type-1 astrocytes were treated with 1×10 exp (2)μM of vinblastine for 24 hours. Cultured type-1 astrocytes damaged by vinblastine showed degenerative changes of cells morphologically. 4. In an electron microscopy, increment of glial fibrillary acidic protein(GFAP), increased free-ribosomes, cisternal dilatation of rough endoplasmic reticulum (rER). few dense bodies and vacuoles were shown in cultured type-1 astrocytes treated with 1×10 exp (2)μM of vinblastine for 24 hours. The results indicate that vinblastine has markedly cytotoxic effect on the type-1 astrocytes of neonatal rat brain in vitro study.
생쥐의 卵子와 初期胚子에 對한 Clomiphene Citrate의 細胞毒性에 關한 硏究
金惠敬,潘勝一,金洋一,文蓮子,朴承澤,鄭然泰 圓光大學校 醫科學硏究所 1993 圓光醫科學 Vol.9 No.1-2
In order to elucidate the cytotoxicity of clomiphene citrate, the rate of in vitro fertilization(IVF) of ova and the developmental rate of early embroys in A-strain mouse were examined. The rate of ova cleavaging to 2-cell stage by IVF was remarkably decreased(12.6%) at the concentration of 10μg/㎖ of clomiphene than that of the control(65.2%) when the ova fertilized in vitro were cultured for 24 hours in the untreated medium after treatment ova with clomiphene for 5 hrs. But there was no ovum cleavaging to 2-cell stage at 50μg/㎖ clomiphene except only a few 2-polar bodied ova. The developmental rate of mouse early embryos was decreased dose-dependently. Especially at 25μg/㎖ of clomiphene, the number of embryos cleavaging to blastocyst from 2-cell stage(41.4%) was decreased remarkably compared with that of the control(87.2%). And only a few embryos were developed to 8-cell stage at clomiphene concentration of 50μg/㎖ after 2-cell embryos were incubated for 72 hours in clomiphene-treated medium. Morphological changes such as fragmentation and fusion of blastomeres, cytolysis and developmental retardation were increased with dose-dependently. These results suggest that clomiphene has cytotoxic effect by the decrease of the rate of IVF, the developmental rate and the degenerative changes of murine ova and early embryos.
Lidocaine, Tetracaine 및 Bupivacaine이 백서 배양 심근세포에 미치는 세포독성에 관한 연구
장정수,최민규,오재민,김종영,정연태 圓光大學校 醫科學硏究所 1994 圓光醫科學 Vol.10 No.1-2
In an attempt to evaluate the cytotoxicity for lidocaine, tetracaine and bupivacaine, beating rates, tetrazolium MTT and lactate dehydrogenase activity were investigated in the medium containing the local anesthetic drugs for 24 hours after the neonatal rat myocardial cells were cultured for 72 hours. Light and electron microscopic studies were also carried out. The results were as follows: 1. Beating rates decreased dose-dependently. and beating myocardial cells were not observed at 10^-3M concentration of tetracaine and bupivacaine, and also beating activities were not recovered after 24hrs. 2. MTT_50 values were 995 μM in tetracaine and 983 μM in bupivacaine. 3. The amount of lactate dehydrogenase released into the medium at 10^-3M concentration were 123% in lidocaine, 156% in tetracaine and 182% in bupivacaine compared with control cells. 4. In the light microscopy, myocardial cells were decreased in number dosede-pendently, and showed a few cytoplasmic processes at 10^-3 M concentration of tetracaine and bupivacaine. 5. In electron microscopy, myocardial cells treated with tetracaine and bupivacaine showed destructed mitochondria and many dense bodies. These results suggest that high concentration of tetracaine and bupivacaine (10^-3M) induce remarkable toxicity compared with lidocaine on the cultured rat myocardial cells.