광주 근교산 붕어의 아미노산 및 지질성분의 분석

李蓮玉,朴貞淑,金忠模,李炳燮,李明烈 조선대학교 기초과학연구소 1992 自然科學硏究 Vol.15 No.1

광주 근교산 붕어의 일반성분, total amino acid 및 free amino arid를 HPLC로 분석 그리고 유리지질과 결합지질을 추출하고 이를 각각 column chromatography에 의하여 중성지질, 당지질 및 인지질로 분획한 다음 이를 각 지질의 구성 지질을 TLC scanner, GC 등으로 분리 정량한 결과는 다음과 같다. 1. 붕어의 조단백질은 5.6%, 조지방은 5.4% 이었다. 2. 총 아미노산 및 유리아미노산은 17종이였으며, 총아미노산은 glycine이 많고 유리아미노산은 arginine이 많이 함유되어 있으며, 필수아미노산량은 총아미노산량의 약 28%를 차지하였다. 3. 총지질 중 유리지질이 5.2% 및 결합지질이 0.2%가 함유 되었으며 유리지질을 구성하는 지질의 함량을 중성지질 81.2%, 인지질 25.5%, 당지질 1.3%로 구성 되였으며 결합지질에는 중성지질 25.5, 인지질 70.0%, 당지길 4.5%로 구성되었다. 4. 유리 및 결합지길 중의 중성지질로는 monoglyceride, diglyceride, free sterol, fatly acid, triglyceride, esterified sterol 등이 분리 동정 되었으며 이중 triglyceride가 유리지질에 82.8%결합지질에 46.1%로 가장 많이 함유되었다. 5. 유리지질과 결합지질을 구성하는 인지질로는 phosphatidyl choline, lecithin, phosphatidylglycerol, phosphatidyl serine, steryl glycoside+phosphatidyl ethanolamine 등이 분리동정 되었으며 유리지질의 경우 phosphatidy1 choline이 2.8%, 결합지질에는 lecithin이 43.5%가 함유되었다. 6. 총지질을 구성하는 주된 지방산은 C_16, _OC18 1의 함량이 가장 많았으며 중성지질은 C_18 2의 함량이 높았으며, 당지질은 유리지질이 C_14 1, C_20 1이 다량 함유되어 있으며 결합지질은 C_20 4,C_18 1이 다량 함유되어 있다. 인지질의 지방산 조성은 C^19_0이 다량 함유되어 있으며 특히 결합지질에는 C_22 6이 다량 함유되어 있다. Approximate compositions, total amino acids and free amino acid, and the isolation and identification of fatty acids in Carassius auratus were analyzed by high performance liquid chromatography, thin layer chromatography and gas chromatography. The results are; Carassius auratus contained 73.8% water, 5.6% crude protein and 5.4% crude fat. 17 kinds of total and free amino acids were analyzed, respectively, glycin is the richest in total amino acids and arginine in free amino acids and total amounts of the essential amino acid were about 28% of total amino acids. Total lipid was contained 5.2% free lipid and 0.2% bound lipid. Free forms and bound forms were mostly consisted of neutral lipids(81.2%) and phospholipid(70.0%), respectively.

Microstructure and High Temperature Oxidation Property of Fe-Cr-B Based Metal/Ceramic Composite Manufactured by Powder Injection Molding Process

Yeun‑Ah Joo,Young‑Kyun Kim,윤태식,Kee‑Ahn Lee 대한금속·재료학회 2018 METALS AND MATERIALS International Vol.24 No.2

This study investigated the microstructure and high temperature oxidation property of Fe–Cr–B metal/ceramic compositemanufactured using powder injection molding process. Observations of initial microstructure showed a unique structurewhere α-Fe and (Cr, Fe)2B form a continuous three-dimensional network. High temperature oxidation tests were performedat 900, 1000 and 1100 °C, for 24 h, and the oxidation weight gain according to each temperature condition was 0.13, 0.84and 6.4 mg/cm2, respectively. The oxidation results according to time at 900 and 1000 °C conditions represented paraboliccurves, and at 1100 °C condition formed a rectilinear curve. Observation and phase analysis results of the oxides identifiedCr2O3and SiO2at 900 and 1000 °C. In addition to Cr2O3and SiO2,CrBO3and FeCr2O4formed due to phase decompositionof boride were identified at 1100 °C. Based on the findings above, this study suggested the high temperature oxidationmechanism of Fe–Cr–B metal/ceramic composite manufactured using powder injection molding, and the possibility of itsapplication as a high temperature component material was also discussed.

소 수정란의 공배양 및 동결이 체외발생에 미치는 영향에 관한 연구

이종진,이명헌,남윤이,이만휘,이봉구,김상근 충남대학교 수의과대학 동물의과학연구소 1996 動物醫科學硏究誌 Vol.4 No.-

The studies on the carried out to investigate the effects of co-culture with cumulus cells and oviduct epithelial cells on the in vitro fertilization and cleavage rate of bovine follicular oocytes, and to determine the optimum thawing temperature and equilibration time of frozen bovine embryos and on survival rate and in vitro developmental rate of bovine embryos. The ovaries were obtained from slaughtered Korean native cows. The follicular oocytes were cultured in TCM-199 medium containing 10 IU/㎖의 PMSG(Sigma, USA), 10 IU/㎖의 hCG, 1 ㎍/㎖의 β-estradiol(Sigma, USA) and 10% FCS(Sigma, USA) for 24-48 hrs in incubator with 5% CO_2 in air at 38.5℃ and then matured oocytes were again cultured for 12-18 hrs with motile capacitated sperm by preincubation of heparin. The bovine embryos following dehydration by cryoprotective agents and a various concentrations of sucrose were directly plunged into liquid nitrogen and thawed in 30℃ water. The results are summarized as followes : 1. The in vitro fertilization and in vitro developmental rates of bovine oocytes co-cultured with cumulus cells in TCM-199 media were 75.0%-76.8% and 17.3%-27.6%, respectively. And in-vitro fertilization rates of cumulus-enclosed oocytes(55.4%) were significantly(p<0.05) higher than cumulus-denuded oocytes(23.1%). 2. The in vitro fertilization and in vitro developmental rates of bovine oocytes co-cultured with 1 ×10^4 cells/㎖, 1 ×10^6 cells/㎖, 1 ×10^8 cells/㎖ and 1 ×10^15 cells/㎖ oviduct epithelial cells in TCM-199 media were 74.5%-77.8% and 15.7%-21.2%, respectively. 3. The in-vitro fertilization and in vitro developmental rates of bovine oocytes co-cultured in TCM-199 media containing PMSG, hCG, PMSG + hCG, PMSG + β-estradiol, hCG + β-estradiol 0 to 40 hrs after insemination were 74.0%-77.4% and 18.9% - 23.1, respectively. 4. The survival rates of bovine embryos thawed after rapid freezing in the media containing various kinds of cryoprotective agents added 0.25M and 0.50M sucrose were 14.7% - 35.1% and 17.6% - 31.6%, respectively. The survival rates of bovine embryos thawed after rapid freezing in the freezing media containing a various concentration of sucrose added 1.5M and 2.0M glycerol, 1.5M and 2.0M DMSO, and 1.5M and 2.0M propanediol were 23.5% -31.4% and 20.6% - 34.1%, respectively. 5. The temperature thawed at 30℃ after rapid freezing of bovine embryos resulted in a significantly higher embryos survival rate than did at 20℃ and 35℃. 6. The equilibration time on the survival rates of bovine embryos was attained after short period of time(2.5-5 min.) in the freezing medium higher than ling period of time(10-20min.).

유아의 인성 함양을 위한 협력학습 활동 프로그램의 평가 연구

이윤옥 서원대학교 교육연구소 1998 敎育發展 Vol.17 No.1

The purpose of this study was to examine the effects of the cooperative learning activity (CLA) program for children. This CLA program for children were developed by Lee Yeun-Ok for the purpose of the encouragement the children's positive personality traits. The subjects of the experimental group were 28 children and the subjects of the control group were 24 children. CLA program was carried out as a whole class activity for one mixed age (3, 4, 5 year old) preschool class for 20 weeks as a experimental group. In this study, four hypothesis were proposed as follows. hypothesis 1 : CLA program group will show higher popularity scores than control group. hypothesis 2 : CLA program group will show higher degree of the number of friend than control group. hypothesis 3 : CLA program group will show higher prosocial behavior scores than control group. hypothesis 4 : CLA program group will show higher school adjustment scores than control group. The outcome of this study were as follows : 1) The hypothesis 1 was rejected. 2) The hypotheses 2, 3, 4 were accepted. These outcome of this study suggest that the CLA Program was effective for the developing the children's positive personaliting traits.

일본의 노인종합복지관 성공사례의 환경지원성 분석 연구

이연숙,이소영,여욱현,장미선 한국의료복지시설학회 2007 의료·복지 건축 Vol.13 No.1

According to the 2005 Korean census, the 65 and over population now exceeds 9.5% of the total population and is growing rapidly. Meeting elderly environment and care needs of this rapidly growing segment of the population becomes a major challenge for public policies and planners. Since great deal amount of elderly will reside in their houses, aging in place concept becomes important. For the success of aging in place, the quality of individual house unit, community support systems, and/or quality of senior center of the community are crucial. Since elderl environments and facilities serve not only medical and/or care programs but also social activity program in aging society, senior centers need to promote social activities and other care programs. The purpose of this study is to analyze characteristics of environmental affordance of a well received senior center in Tokyo, Japan. In order to analyze the characteristics, Murtha & Lee user benefit criteria and Lawton's environmental affordance approaches were used. As results, design characteristics and interior elements which provide environmental affordance were enumerated by type of space. Based on needs and user benefit criteria, those features were analyzed. This study shows design characteristics, elements, and attributes which are well received and utilized by elderl users.

노화로 인한 신체적 기능 쇠퇴와 고령자를 위한 욕실 디자인 가이드라인 관계 연구

이연숙(Lee, Yeun-Sook),임현진(Lim, Hyun-Jin),이지혜(Lee, Ji-Hye),안창헌(Ahn, Chang-Houn) 한국실내디자인학회 2013 한국실내디자인학회논문집 Vol.22 No.5

Advancement toward aging society has presented the importance of house planning in consideration of the elderly, increasing the necessity of such planning. Therefore the importance of indoor design guideline for elderly houses has been stressed and the understanding of the aging in regard to this trend will create more mature spaces. In line with this trend the purpose of this study is to study the medical factors related with bathroom interior design guidelines of the existing elderly houses from the viewpoint of deteriorating physical functions due to aging process. In order to research the deterioration of physical functions due to aging literatures of medical and exercise physiology were used and concerning the existing bathroom design guidelines for the aged people, the guideline items of behavior facilitation, physiological maintenance and perceptual maintenance aspects out of Murtha & Lee’s user benefit criteria(1976) related with physical functions were selected to research in relation with the knowledge about the deterioration phenomena of physical functions. Physical aging and deterioration aspects were classified from the viewpoint of musculoskeletal disorder, cardiovascular disorder, respiratory disorder, gastronomy disorder, urology disorder, somatosensory disorder, endocrine disorder, immune disorder, nervous disorder and skin diseases and these were utilized in interpreting total 100 items of bathroom design guideline. Because bathroom is the space where many physical movements are done, it had the closest relationship with the deterioration of musculoskeletal health in general and as bathroom is the space where people use water and feel the difference in temperature and moisture more than other spaces, deterioration of skin and somatosensory health had the next closest relationship. The result of this study revealed that regarding the deterioration of physical functions of the elderly people in aspect of their perceptibility as designers will design creatively and sincerely based on its relationship with nervous system in future, the result of this study will be used to develop better spatial designs efficiently to meet for the aging society.

또래수용도 증진을 위한 사회정보처리 모델의 적용 : Focused on the Social Maladjustment Behavior in Peer Groups

이윤옥 서원대학교 교육연구소 2001 敎育發展 Vol.20 No.1

The last two decades have witnessed rapid progress in research and theory regarding the contributions of social information processing(Crick & Dodge, 1994 ; Dodge, 1986)and social behavior. Although both research traditions share a focus on social competence, integration across the domains of social information processing and peer acceptance in the early years has been minimal. So this study examined that an essential aspect of this broader view involves considering, both theoretically and empirically, how cognitive processes and social behavior can be integrated in models of social competence. The purpose of this study was to development an Integrated Model of Social Behavior and Peer Acceptance in Social Information Processing in the context of peer group. Crick & Dodge's model was presented as a cognitive model of social decision making, and the relationships between social competence types and psychosocial adjustment was reviewed. In addition, Children's social information-processing patterns and social characteristics was examined. The revised model is proposed into which social behavior and cognitive processes can be integrated in models of social competence.

유아의 블록놀이를 통한 놀이문화의 연구

이수남,오연주 안양대학교 인문과학연구소 2001 人文科學硏究 Vol.9 No.-

The purpose of this study was interested in the inter-cultural relation of play participants through the block of the young children, and tried to find out the rule of the play in social system and their experiences of the young children. This study inverstigated how the young children solve their problems through the block play, how they communicate, how the symbolic play is developed, and how behavior patterns of children are categorized. The rules were as follows : 1. The symbolic play was developed with the types of block play. 2. The small things - dinosaurs mode, insects mode - were important role to develop the symbolic play of the young children. 3. Children were interested in making stages for symbolic play rather than doing symbolic play. 4. The structure of paper block had not only provided the stage of the symbolic play but also related the structure of story-telling. 5. There were moral rules of children in their play.

유아미술 교육과 과학교육의 통합적 접근 연구 : - 통합적 미술 교육 프로그램 구성을 중심으로 -

이윤옥,임창옥 서원대학교 2001 교육논총 Vol.5 No.-

This study was to develop integrated approach program mixed of art and science curriculum (ASIAP). The purposes of this treatise are as follow : First, As society and culture are getting rapidly changed in the whole situation, We need to try to facilitate the integrated art education centered on themes of scientific concept applied creative art education methods of Barnes and inquiry science method based on constructivism. Second, According to the Lim, Chang-ok(2000), children who have experienced the integrated activity-centered art and science education showed a meaningful difference in the scientific inquiry ability and attitude. Therefore, We need to develop the integrated activity focused on art education improving scientific inquiry ability and attitude. Third, To facilitate how can teachers integrate activity into the classroom for kindergartners, We need to describe how integrate theory convert into the praticum and explain the common elements of integrated curriculum-program coals and objectives, contents, instructional strategies, evaluation. It concluded that the ASIAP program was the efficient curriculum could be applied through a theme immersion in early childhood education field

미국 공립유아원 Head Start 프로그램의 개별화교육방안에 대한 고찰

이윤옥 서원대학교 교육연구소 2000 敎育發展 Vol.19 No.1

This study explores the Individualized Education program(IEP), based on the Head start program's curriculum of the Montgomery public schools(MCPS). The purpose of this study is to provide information on the Instructional philosophy/conceptual Framework about the individualizing in Head Start program in terms of the assumption, performance principle, process, guideline. Individualizing is basic to the philosophy of Head Start. It is an ongoing process that continues throughout a child and family's participation in Head Start. The Individualizing Cycle in Head Start consists of the interrelated four steps; Screening, Evaluation, Planning for Individual Children, Ongoing Assessment. It is designed to further develop the skills of the education staff. It contains the following modules; Learning about each child, Creating essential partnership-families and staff working together, An ongoing continues. The benefits of an individualized approach are easy to get fully involved in activities, actively explore their environments, feel proud when they can do things for themselves, and enjoy playing and learning with others. This study contributes to the teacher to use a variety of strategies to get to know and plan for children, build partnership with families and asses children's progress in Korean early childhood education settings.