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( Yeoung Gyu Ko ),( Dong Hun Kim ),( Soo Bong Park ),( Sung Woo Kim ),( Yoon Jung Do ),( Hyun Kim ) 한국동물자원과학회 ( 구 한국축산학회 ) 2012 한국축산학회지 Vol.54 No.3
Sloan-Kettering virus gene, a product of a cellular protooncogene c-Ski is a unique nuclear pro-oncoprotein and belongs to the Ski/Sno proto-oncogene family. The aim of the present study was to locate Ski protein in rat ovaries in order to find insights into the possible involvement of Ski in follicular development and atresia. Ovaries obtained on the day of estrus were subjected to immunohistochemical analysis for Ski and PCNA in combination with TUNEL. Ski was expressed in granulosa cells that were positive for TUNEL, but negative for PCNA, regardless of the shape and size of the follicles. Expression of Ski in (TUNEL)-positive granulosa cells, but not in(PCNA)-positive granulosa cells, was verified in immature hypophysectomized rats having a single generation of developing and atretic follicles by treatment with equine chorionic gonadotropin(eCG). These results indicate that Ski is profoundly expressed in the granulosa cells of atretic follicles, but not in growing follicles. Based on the present findings, Ski has maybe played plays a role in apoptosis of granulosa cells during follicular atresia.
Gyu-Tae Yeom,Hae-Geum Park1,Nam-Tae Kim,Sung-Woo Kim,Hyun Kim,Yoon-Jung Do,Young-Sin Kim,Soo-Bong Park,Jae-Hwan Kim,Sang-Rae Cho,Jae-Hyeon Cho,Yeoung-Gyu Ko 韓國受精卵移植學會 2013 한국동물생명공학회지 Vol.28 No.1
Body condition score (BCS) is a useful management tool for distinguishing differences in nutritional needs of cows in the herd. Although it is not always possible to quantify the nutrient content of the feed supplied to the donor cow, the nutritional status can be determined by the BCS. The objective of this study was to evaluate in vivo embryo production, return to estrous of donor and pregnancy rate of recipients following BCS in Hanwoo superovulation. Sixty nine Hanwoo donor cows were flushed on day 7 of estrus cycle with same FSH and artificial insemination by the same technicians. Embryos were recovered on 7 days after the third insemination by flushing the uterus with Embryo Collection Medium. The results obtained were as follows: No differences were observed in the efficiency of superovulation rates regardless of BCS (≤2.0, 2.5 to 3.0, and ≥3.5). The mean number of total embryos was each 5.20±0.86, 11.56±1.04, and 6.23±1.07. The mean number of transferable embryo from ≤2.0, 2.5 to 3.0, and ≥3.5 of BCS was 2.60±0.87, 7.94±0.89, and 4.75±1.32, respectively (p<0.05). Return to estrous regardless of donor BCS was no difference. The pregnancy rates of recipient were BCS ≤2.0 11.76%, 2.5 to 3.0 40.79%, and ≥3.5 11.11%, following transfer of fresh embryos produced in vivo, respectively. These results indicate that if the Hanwoo with BCS 2.5 to 3.3 are used for donor and recipient, the embryo production and the conception rate will be greater.
The Expression of Imprinted Genes in Porcine Conceptus during Peri-implantation Development
Yeoung-Gyu Ko,Hwi Cheul Lee,Byoung Chul Yang,Seongsoo Hwang,Gi Sun 1m,Jae Seok Woo,Poongyeon Lee,Changyeon Cho,Sang Woo Kim,Sun Ho Choi 한국동물번식학회 2010 Reproductive & Developmental Biology(Supplement) Vol.34 No.2s
The Studies on In Vivo Embryo Production using Sex-Sorted Sperm in Hanwoo
Yeoung-Gyu Ko,Sung Woo Kim,Dong-Kyo Kim,Nam-Tae Kim,Chan-Lan Kim,Ik-Soo Jeon,Min-Su Kim 한국수정란이식학회 2018 한국수정란이식학회 학술대회 Vol.2018 No.11
The present study was to assess the in vivo embryo production efficiency using the semen separated according to sex during superovulation in Hanwoo. Seventy Hanwoo donor cows were flushed on day 7 of estrus cycle with same FSH and artificial insemination by the same technicians. Embryos were recovered on 7 days after the third insemination by flushing the uterus with embryo collection medium. KPN semen straws used artificial insemination contained 20 million sperm (total number 60 million per donor). Sex-sorted semen straws contained 4 million sperm (total number 12 million per donor). The results obtained were as follows: No differences were observed in the efficiency of superovulation rates on KPN semen 87%, and sexed semen 100%, respectively. The mean numbers of total embryos are each 12.58 ± 8.31 and 13.25 ± 7.86. The mean numbers of transferable embryos, sexed semen were significantly lower than KPN semen (3.75 ± 1.98 vs. 8.23 ± 6.07, P<0.05). The rates of unfertilized embryos from superovulation using sexed semen were significantly higher than KPN semen (50% vs. 15%, P<0.05). The rate of degenerated 2-cell embryos from sexed and KPN semen was 60.87% and 11.11%, respectively (p<0.05). In conclusion, these results indicate that superovulation using sexed semen was useful, but efficient embryo production was important to reducing the damage caused by the Flowcytometer-based sperm sorting procedure.