스모그 챔버에서 수분 반응에 의한 대기 에어로졸의 생성 및 성장

김민철,배귀남,문길주,박주연 한국대기환경학회 2004 한국대기환경학회지 Vol.20 No.2

Aerosol formation and growth by water vapor reactions were investigated in a 2.5-㎥ indoor smog chamber filled with the unfiltered ambient air. The relative humidity of test ambient air was elevated at 59~64% or 84~88% by adding water vapor. The aerosol number size distribution and the concentrations of O₃, NO, NO₂, and SO₂ were measured during the experiments. The O₃, and NO₂ gases were well reacted with the water vapor at high relative humidity of 84~88%, and the reaction rates of these gases seemed to be decreased at low relative humidity of 59~64%. The formation and condensational growth phenomena of ambient aerosols by water vapor reactions were observed in a Teflon bag, depending strongly on the initial particle size distribution. The water vapor reactions might be affected by the contents of oxidants produced by photochemical reactions under sunlight.

출류(朮類)한약재의 외,내부형태와 이화학패턴 연구

김정훈 ( Jung Hoon Kim ),이금산 ( Geum San Lee ),최고야 ( Go Ya Choi ),황성연 ( Sung Yeoun Hwang ),김홍준 ( Hong Jun Kim ),정승일 ( Seung Il Jeong ),주영승 ( Young Sung Ju ) 대한본초학회 2009 大韓本草學會誌 Vol.24 No.2

Objectives: To determine the discriminative criteria for Atractylodes rhizomes, the experiment of external-internal characteristics and physicochemical pattern analysis were performed. Methods: External characteristics was observed using stereoscope. The sectioned materials which were covered with parffin were stained by Ju`s method. Physicochemical patterns were analyzed using HPLC/DAD. Results: 1. External shape of original plant: Atractylodes macrocephala and A. japonica had relatively long petioles and 3-5 parted leaves. A. macrocephala had big purple flowers whereas A. japonica had relatively small white flowers and pinnate bracts. A. lancea had sessile leaves and white flowers, and the end parts of degenerated stamens were bent. 2. External shape of herbal medicine: A. macrocephala which was fist-shaped rhizome had pa-pillate processes and the cross section was light gray and sulcate. A. japonica and A. lancea were connected-beady or tubercular rhizomes, and the cross sections were both yellow-colored white. However, the cross section of A. japonica was fibrous, the width of cortex was narro-wer than that of stele, and radial shape in cortex was rare, whereas the width of cortex in A. lancea was similar to that of stele in size, and radial shape in cortex was obvious. 3. Internal shape of herbal medicine: A. macrocephala and A. lancea did not have lignified fascicles in cortex. However, the vascular bundles and vessels of A. macrocephala were wedge shaped and radial arrangement, and vascular bundles were densely populated in stele whereas those of A. lancea were repeatedly arranged and thinly extended to cortex. A. japonica had lignified fascicles in cortex and the width of vascular bundles was conspicuously thick with narrow intervals. 4. Physicochemical pattern analysis: A. macrocephala and A. lancea contained atractylenolide Ⅰ and atractylenolide Ⅲ whereas A. japonica contained atractylenolide Ⅰ, atractylenolide Ⅲ, diacetyl-atractylodiol, compound-4, compound-5. The three species of Atractylodes rhizomes showed different chromatogram patterns. Conclusions: The results could be used as discriminative criteria for Atractylodes rhizomes and as fundamental materials to researches of further pattern analysis and biological reaction.

후박(厚朴)의 외,내부형태 및 이화학패턴연구

이금산 ( Guem San Lee ),김정훈 ( Jung Hoon Kim ),최고야 ( Go Ya Choi ),강대훈 ( Dae Hoon Kang ),황성연 ( Sung Yeoun Hwang ),정승일 ( Seung Il Jeong ),김홍준 ( Hong Jun Kim ),주영승 ( Young Sung Ju ) 대한본초학회 2008 大韓本草學會誌 Vol.23 No.4

Objectives: To determine the standards for discrimination of Magnoliae Cortex, the experiment of specific external-internal characters and the physicochemical pattern analysis were performed. Methods: External characteristics was observed using a stereoscope. Paraffin-mediated sectioned materials were stained by Ju`s method. Physicochemical patterns of materials were analyzed using HPLC. Results: 1. Botanical characteristics: Magnolia officinalis had one seed and a white flower, while M. obovata had two seeds and a white flower. Machilus thunbergii had berry and spherical fruits and yellowish green panicles. 2. External characteristics: M. officinalis and M. obovata were dark and thick. M. officinalis was gray brown and greasy while M. obovata was light-gray, less oily and smoothly sectioned. Machilus thunbergii was thin and relatively light or yellow-brown, coarsely sectioned and faintly specific scents. 3. Internal characteristics: The bast parts of M. officinalis and M. obovata were commonly wider than Machilus thunbergii. The cork cortex of M. officinalis was 10~mg/L cell layers with many oil cells, while that of M. obovata was 4~7 cell layers with less oil cells. Machilus thunbergiis xylem which consisted of ring-shaped cambium at 1st and 2nd part was occupied in large portion. 4. Physicochemical pattern: Both M. officinalis and M. obovata involved honokiol and magnolol. All kinds of M. officinalis involved Magnatriol B but one kind of M. ovobata and all of Machilus thunbergii didn`t. Machilus thunbergii showed different pattern of chromatogram from that of 2 species above. Conclusions: These results could be used as standards for discrimination of Magnoliae Cortex and as the method of objectification in medicinal herbs giving the basic resource for bioactivity research.

Genome size estimation of the chigger mite, Leptotrombidium pallidum, based on quantitative real time PCR

Ju Hyeon Kim,Jong Yul Roh,E-hyun Shin,Mi-Yeoun Park,Si Hyeock Lee 한국응용곤충학회 2013 한국응용곤충학회 학술대회논문집 Vol.2013 No.10

The chigger mite, Leptotrombidium pallidum, is widely distributed throughout South Korea and is a major vector for Orientia tsutsugamushi, the causative agent of scrub typhus. In this study, the genome size of the chigger mite was estimated to determine the necessary coverage level prior to whole genome sequencing. Cloning of EF1α and RpS3 as putative single copy reference genes were conducted and their partial sequences were determined. Using the serially diluted reference genes with known amount as standard templates, the weight of a single copy of the genome was predicted by a method based on quantitative real time PCR. The average genome length estimated from the weight using two methods was 191 ± 7 Mb. When the genome size of other arthropods (Drosophila melanogster, Apis mellifera and Tetranychus urticae), with their genome analysis completed, were estimated using the same method and compared with actual values, the estimation accuracy was 79.8-98.9%, suggesting our current estimation of L. pallidum genome size is reliable. The estimated L. pallidum genome size is in a similar range to other Acariform mites, such as the dust mite and scabie mite, but appoximately 10-fold smaller compared to the deer tick, which belongs to Parasitiform. Our finding provides key information for further genome sequencing and understanding of mite genome evolution.

The Characteristics of Bacillus Species Isolated from Korean Traditional Soy Sauce, Ganjang

Yeoun-Ju Choi,Ju Hee Yoon,Ji Hyeon Kim,Kiwon Lee 한국식품영양과학회 2022 한국식품영양과학회 학술대회발표집 Vol.2022 No.10

C21 Steroidal Glycosides from the Root of Cynanchum paniculatum

Ju Yeoun Oh,김충섭,이강노 대한화학회 2013 Bulletin of the Korean Chemical Society Vol.34 No.2

Current Updates on the Genome Analysis of Leptotrombidium pallidum, a Major Vector Mite for Scrub Typhus

Ju Hyeon Kim,Jong Yul Roh,Deok Ho Kwon,Young Ho Kim,Kyungjae A. Yoon,Seungil Yoo,Yoonhee Shin,Seung-Jae Noh,E-hyun Shin,Mi-Yeoun Park,Young Ran Ju,Si Hyeock Lee 한국응용곤충학회 2014 한국응용곤충학회 학술대회논문집 Vol.2014 No.10

Leptotrombidium pallidum is the major vector mite for Orientia tsutsugamushi, the causative agent of scrub typhus, in Asian countries, including Korea. The genome size of L. pallidum was previously estimated to be 191 ± 7 Mb (Kim et al., 2014). Genomic DNA (gDNA) was extracted from a single female from a 9-generation inbred L. pallidum colony and used for whole genome amplification (WGA). The resulting amplified gDNA was used for the construction of paired-end and mate-pair libraries and sequenced using Illumina platforms (HiSeq2000 and MiSeq). An unamplified gDNA sample extracted from 20 female mites was also used for sequencing in parallel. More than 45Gb sequence reads from both paired-end and mate-pair libraries of the WGA gDNA were trimmed and then de novo assembled using the CLC Asembly Cell v.4.0 for contig assembly and SSPACE for scaffolding. The assembly generated approximately 6,545 scaffolds with N50 value of 92,945 and total size of ~193Mb, which was in a good agreement with our previous estimation. Repeat analysis showed that about 30% of genome (~58Mb) was masked as repeats, most of which were unclassified novel elements. For gene predictions, generated were the PASA models based on genomic alignments of RNA-seq reads from 4 different chigger mite samples (i.e. male, female, larva, and protonymph) and the GeneWise models based on genomic alignments of protein sequences from 4 closely related species with chigger mite. Independently, ab initio gene predictions were performed with AUGUSTUS and FgeneSH with custom trained matrices optimized for L. pallidum and GENEID with pre-trained matrix for Acyrthopsiphon pisum. By combining all together, 15,842 genes were predicted finally. Manual curation is in progress for various groups of genes, including chemosensory receptor genes, immune-related genes, acaricide target genes, etc.

운전 중 사용자의 의사결정 컨텍스트를 고려한 HUD 네비게이션 디자인 연구

나주연(Ju Yeoun Na),백소담(So Dam Baek),허정윤(Jeong Yun Heo) 한국HCI학회 2017 한국HCI학회 학술대회 Vol.2017 No.2

최근 HUD(Head-Up-Display)인터페이스에 대한 연구가 활발히 진행되고 있지만 주행 중 운전자의 주의가 다르게 적용되는 구간이 존재하는 반면, 운전자의 주의 집중도를 고려한 HUD 인터페이스에 대한 연구는 미흡한 실정이다. 본 연구에서는 주행 중 나타나는 사용자의 여러 의사결정 행태를 분석하고 사용자의 주의가 가장 크게 요구되는 좌/우회전 컨텍스트를 중심으로 HUD(Head-Up-Display)디자인 네비게이션 디자인을 제안한다. 문헌조사를 바탕으로 연구의 이론적 바탕이 되는 HUD 의 특징과 운전자의 주의인지, 어포던스의 개념에 대하여 고찰하고 실제 운전자의 의사결정 컨텍스트에 요구되는 주의 정도에 대한 설문조사를 통하여 주행 중 운전자의 주의가 높게 요구되는 ‘좌/우회전’컨텍스트를 도출하였고 주의요구도가 다른 주행 행태에 따라 차별화된 인터페이스 디자인 제공의 필요성을 제기하였다. 이와 더불어, 모바일 네비게이션 어플리케이션의 사례분석과 기존 차량용 HUD 디자인 가이드와 관련된 다양한 연구 레퍼런스를 토대로 실제 차량용 네비게이션 인터페이스를 구성하고 있는 시각적 어포던스의 표현방법을 벤치마킹 하였다. 이를 반영하여 운전자의 주행 중 의사 결정 상황에 맞는 차별화된 차량용 HUD 인터페이스를 구성하는 디자인가이드를 제안하였다.

논문 : 구조와 즉흥의 관점으로 바라본 교육 연극 실천들

김주연 ( Ju Yeoun Kim ) 한국연극교육학회 2010 연극교육연구 Vol.17 No.-

There are many various practices of educational drama in Korea. With the explosion of those practices, it is timely useful to interpret these practices to understand what they really are. Borrowing `structure and spontaneity` which represent O`Neill`s `process drama`, this study analyses Slade`s `child drama`, Way`s `exercise`, Heathcote`s `mantle of the expert`, Sadari dramatic play, Haemaru dramatic play, Sogubnori`s educational drama and performing theatre using dramatic play. Process drama is structured with `living through` approach, text, and theatrical devices to help students to experience spectatorship, transformation, ritual and so forth. Child drama employes story, music or rhythm to stimulate students` physical movement to help students experience the significance of form. Excercise aims for students` growth for whole person so that it makes use of exercises for imagination, speaking, sensitivity, physical self, emotion, intellect, concentration and senses. Mantle of the expert is structured with living through approach, teacher-in-role, taking role of expert to help students acquire knowledge and skills of curriculum. Sadari dramatic play is made in a way to strengthen or facilitate dramatic play. Haemaru dramatic play pursues `hung` or `shinmyoung puri` so that they empoyes `changdan` `breathing` whole participation and so on. Sogubnori`s educational drama aims at students` learning and therefore, it controls and manipulates students` play. Performing theatre using dramatic play proceeds with students` play. Having analyses these practices, it is found that they all share spontaneity but structure differs according to teachers` perspectives. Besides such perspectives affect the relationship between structure and spontaneity. In exercise, mantle of the expert, Sogubnori`s educational drama, structure is more emphasized than spontaneity. Spontaneity is controlled or governed by structure. However, in child drama, process drama, Sadari dramatic play, Haemaru dramatic play, performing theatre using dramatic play, structure and spontaneity is well balanced or spontaneity is more stressed. Having analysed these practices, it is uncovered that exercise, mantle of the expert and Sogubnori`s educational drama are similar in terms of facilitating learning through play but other practices are different in terms of their viewpoint of aesthetic experiences. Acknowledging such diversity, there is a question that how such diversity can be gathered in the filed of educational drama. The common ground is spontaneous and voluntary play. That is to say, it is based upon the belief that human nature is naturally good.

간호대학생의 격리주의지침에 대한 지식과 태도

송주연 ( Ju Yeoun Song ),정인숙 ( Ihn Sook Jeong ) 부산대학교 간호과학연구소 2013 글로벌 건강과 간호 Vol.3 No.2

on nursing students. Methods: Cross-sectional survey was done with a self-administered questionnaire from 350 nursing students in 8 college from December 19, 2011 to February 21, 2012. Results: Overall correct answer to 13 knowledge-type questions was 70.8% percent, which wasn`t significantly different ever taken clinical practice (p=.174). 236 nursing students had taken an infection control training, which was significantly different (p=.012). The question ``the most important vehicle in pathogen transmission`` showed the highest correct answer (94.9%), followed by ``risk-guided application of a preventive strategy`` (92.0%). The question ``the main purpose of glove use`` showed the lowest correct answer (28.3%), followed by the basic concept of standard precautions` (34.6%). Preferred single prevention measure was ``hand hygiene`` (98.6%). 47.5% of the respondents answer ``know vaguely`` on the question about ``self assessment of knowledge on the concept of isolation precaution``. Preferred source of information on isolation precautions was searching for guideline (62.6%) followed by calling the hotline (24.8%). The most important obstacle to compliance with isolation precaution guideline was forgetfulness (65.4%), followed by lack of time (42.9%), lack of knowledge (34.3%), and lack of needs (2.6%). Conclusion: The knowledge on the isolation precautions among nursing students was low, therefore nursing students require fundamental and comprehensive courses in infection control precautions prior to taking part in the clinical practice. Considering forgetfulness was the most important obstacle to adhere to isolation precaution guideline, repeated education in the schools or hospitals are also recommended.