Association of rs10757274 and rs2383206 Polymorphisms on 9p21 locus with Coronary Artery Disease in Turkish Population

Çağrı Yayla,Kaan Okyay,Asife Şahinarslan,Akın Yılmaz,Atiye Seda Yar Sağlam,Azmi Eyiol,Hasan Ata Bolayır,Burak Sezenöz,Sevda Menevşe,Atiye Çengel 대한심장학회 2016 Korean Circulation Journal Vol.46 No.5

Background and Objectives: Genetic predisposition is an important risk factor for coronary artery disease (CAD). In this study, we aimed to evaluate the impact of rs10757274 and rs2383206 polymorphisms in chromosome 9p21 on presence and severity of CAD in a Turkish population. Subjects and Methods: A total of 646 patients who underwent coronary angiography were included in this study. Coronary vessel score and Gensini score were calculated to assess the angiographic severity of CAD. Alleles of AA, AG, and GG were determined for rs10757274 (polymorphism-1) and rs2383206 (polymorphism-2) polymorphisms located in chromosome 9p21 from the blood samples. Results: There was a significant difference between the alleles in polymorphism-1 in the presence of coronary artery disease (38.9% in AA, 48.0% in GG and 56.4% in AG, p=0.017). However, there was no difference between the alleles in polymorphism-2. According to vessel scores, there was a significant difference between the alleles in polymorphism-1 (AA 0.71±1.04, GG 0.88±1.07, AG 1.06±1.12, p=0.018). In polymorphism-2, vessel scores did not show a difference between the alleles. In polymorphism-1, there was a significant difference in Gensini score (p=0.041). Gensini scores did not differ between the alleles in polymorphism-2 (p>0.05 for all). In multivariate analyses, none of the alleles was an independent factor for presence of CAD. Conclusion: The presence of rs10757274 polymorphism including AG allele in chromosome 9p21 was related to CAD. However, this relationship was not independent of other cardiovascular risk factors.

CASE REPORT : Two Cases of Unilateral Lichen Planus Following the Lines of Blaschko

( Derya Yayla ),( Seray Kulcu Cakmak ),( Isıl Deniz Oguz ),( Muzeyyen Gonul ),( Esra Ozhamam ),( Aysel Colak ),( Ulker Gul ) 대한피부과학회 2014 Annals of Dermatology Vol.26 No.5

A 50-year-old man and 71-year-old woman presented to ourclinic with unilateral, linear, erythematous, pruritic lesions along the lines of Blaschko. On the basis of clinical andhistopathological findings, the lesions were diagnosed as lichen planus with a Blaschkoian distribution, which is a rare form of lichen planus. The patients were treated with topical corticosteroids and antihistamines. (Ann Dermatol 26(5) 636 ∼638, 2014)

Investigation of human papillomavirus prevalence in married women and molecular characterization and phylogenetic analysis of the virus

Ayse Erdem Yayla,Berrin Goktug Kadioglu,Ayse Aydin,Osman Aktas 대한산부인과학회 2019 Obstetrics & Gynecology Science Vol.62 No.4

ObjectiveHuman papillomaviruses (HPVs) are among the agents responsible for infection and cancer of the skin and mucousmembranes in the human body. The aim of this study was to investigate the frequency and type distribution of HPVsin married female patients with gynecological complaints, who had visited the Maternity Hospital in Erzurum, Turkey. MethodsIn this study, 263 cervical swab samples were taken from married women using the Pap smear method and wereinvestigated for positive reactivity against HPV. The L1 gene region of HPV was investigated using molecular methods. For this purpose, polymerase chain reaction (PCR) assays and sequence analysis of positive samples were performed. Phylogenetic analyses were performed using a bioinformatics approach after sequencing. ResultsHPV-DNA was detected in 17 (6.5%) samples. Highest positive reactivity to HPV-DNA was found in the 35–44 agegroup at 9.2%. Fourteen out of seventeen positive samples were included in the phylogenetic analysis. All isolatesclustered in the Alphapapillomavirus genus. Six samples were found to be HPV 70 positive, four were HPV 16 positive,and the rest were HPV 54, 72, 81, and 114 positive. When genotyping data were evaluated according to the riskgroup, we found that 28.6% of the 14 samples were found to be high risk-HPV, and 71.4% were low risk-HPV. ConclusionAs per our knowledge, this is the first report on the phylogenetic analysis of HPV genotypes isolated from women inTurkey. The prevalence of low- and-high risk HPV was determined in married women in Erzurum, and these resultscontribute to the epidemiological data on the distribution of HPV types for this region.

Serum Irisin Level Can Predict the Severity of Coronary Artery Disease in Patients with Stable Angina

Tolga Han Efe,Çağrı Yayla,Burak Açar,Göktuğ Ertem,Kadriye Gayretli Yayla,Engin Algül,Sefa Ünal,Murat Bilgin,Tolga Çimen,Özgür Kirbaş,Ekrem Yeter 대한심장학회 2017 Korean Circulation Journal Vol.47 No.1

Background and Objectives: The recently discovered myokine irisin has a proposed role in adipose tissue metabolism. The aim of this study was to evaluate the relationship between serum irisin level and the coronary artery severity in patients with stable coronary artery disease (CAD). Subjects and Methods: Sixty-three patients who underwent coronary angiography (CA) diagnosed with stable CAD and twenty-six patients with normal coronary artery (NCA) were enrolled in the study. Stable CAD patients were divided into two groups as high synergy between percutaneous coronary intervention with taxus and cardiac surgery (SYNTAX) score (≥23) and lower SYNTAX score (<23). Serum irisin level measurement was carried out using human irisin colorimetric enzyme-linked immunosorbent assay (ELISA) commercial kit (AG-45A-0046EK-KI01, Adipogen, San Diego, CA, USA) as recommended by the manufacturer’s protocol. Results: The patients with stable CAD with a higher SYNTAX score (score ≥23) had significantly lower serum irisin levels (127.91±55.38 ng/mL), as compared the patients with a low SYNTAX score (score <23) (224.69±92.99 ng/mL) and control group (299.54±123.20 ng/mL). Irisin levels showed significant differences between all groups (p<0.001). Conclusion: Serum irisin level is an independent predictor of coronary artery severity in patients with stable CAD.

Portal Hypertension in Children: A Tertiary Center Experience in Turkey

Emine Nur Sunar Yayla,Sinan Sarı,Neslihan Gürcan Kaya,Ödül Eğrİtaş Gürkan,Hakan Sözen,İbrahim Onur Özen,Aydın Dalgıç,Buket Dalgıç 대한소아소화기영양학회 2023 Pediatric gastroenterology, hepatology & nutrition Vol.26 No.6

Purpose: Portal hypertension (PH) and its complications have a significant impact on morbidity and mortality. This study aimed to evaluate the etiology; clinical, laboratory, and endoscopic findings; treatment approaches; long-term outcomes; and prognosis of pediatric PH. Methods: This retrospective study included 222 pediatric patients diagnosed with PH between 1998 and 2016, and data encompassing clinical, laboratory, and radiological features; treatments; and complications were analyzed. Results: The most common causes of PH were portal vein thrombosis (20.3%), progressive familial intrahepatic cholestasis (18.9%), and biliary atresia (12.2%). Among the enrolled patients, 131 (59.0%) were included in the cirrhotic group and 91 (41.0%) in the noncirrhotic group. Hepatomegaly and increased transaminase levels were more frequent in the cirrhotic group than in the non-cirrhotic group. Additionally, portal gastropathy, esophageal varices, and variceal bleeding were more frequent in the non-cirrhotic group, whereas ascites, hepatopulmonary syndrome and hepatic encephalopathy were more common in the cirrhotic group. The incidence of hepatomegaly was higher in the presinusoidal group than in the prehepatic group (p<0.001). Hyperbilirubinemia was more frequent in the prehepatic group (p=0.046). The frequency of esophageal varices was similar between the prehepatic and presinusoidal groups; however, variceal bleeding was more frequent in the prehepatic group (p=0.002). Conclusion: Extrahepatic portal vein obstruction, genetic-metabolic diseases, and biliary atresia were the most prevalent causes of PH in our country. In patients with PH, hepatomegaly, increased transaminase levels, and synthesis dysfunction were suggestive of cirrhotic PH. Notably, PH in patients without cirrhosis might be more severe than that in those with cirrhosis.

Effects of flexible plate attached to the rear of the cylinder on flow structure

Suleyman Teksin,Sedat Yayla 대한기계학회 2017 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.31 No.3

In this experimental study, the flow structure in the wake flow region was investigated with the Particle image velocimetry technique (PIV) by attaching elastic plates at different lengths behind the cylinder. The flow structure occurred at the wake flow region altered depending on the length of the flexible matter. In this experiment, the strips with the lengths of 75, 90, 120, 135 and 180 mm were used to control instabilities. Diameter of the cylinder (D) is 60 mm and the water height (h w ) is 600 mm. Reynolds number was kept constant as 5000 based on cylinder diameter. The images were captured at mid-height of the cylinder (h m ) which is 250 mm. As a result of experimental studies, attached flexible strip suppressed vortex shedding occurred in the behind of the cylinder and it is observed that effect of the length flexible of the strip is pretty much. Maximum level of flow characteristics such as Reynolds stress, fluctuation velocities and turbulent kinetic energy were decreased with flexible splitter plate and shifted through the downstream region.

Methacrylate Polymers Having Pendant Chalcone Moieties

Gamze Barim,Ozgul Altun,Mustafa Gokhun Yayla 한국고분자학회 2015 폴리머 Vol.39 No.1

A new methacrylate copolymer that includes chalcone as a side group, poly(4-methacryloyloxyphenyl-4"-methoxystyryl ketone-co-styrene) was synthesized by free radical copolymerization. FTIR and 1H NMR spectroscopic techniques were used to characterize the homopolymers and copolymers. The copolymerizations were carried out to high conversions. Copolymer compositions were established by 1H NMR spectra analysis. The monomer reactivity ratios for copolymer system were determined by the linearized Kelen Tudos, and Extended Kelen Tudos methods and a non-linear least squares method. The molecular weights and polydispersity index of copolymers were measured by using the gel permeation chromatography (GPC). The effect of copolymer compositions on their thermal behavior were studied by differential scanning calorimetry and thermogravimetric analysis methods. The optical properties of the resulting copolymer were also investigated.