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Yayi Cheng,Jianfeng Huang,Liyun Cao,Yongfeng Wang,Ying Ma,Shaohua Xi,Bingyao Shi,Hui Xie,Jiayin Li 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2019 NANO Vol.15 No.01
SnSe2 and SnSe nanocrystals were prepared using a simple solvothermal method by changing the molar ratio of SnCl2 · 2H2O and Se powder. When SnSe2 and SnSe are acted as lithium ion battery anodes, the SnSe hybrid structure shows more excellent electrochemical performance than that of SnSe2 interconnected nanosheet. It delivers a reversible capacity of 1023 mA h g -1 at a current density of 200 mA g -1, and maintaining a capacity of 498 mA h g -1 till 120 cycles. According to many present works, SnSe2 with interconnected thin nanosheet should possess more superior property than hybrid structured SnSe due to short charge transfer paths. However, in our research, the result is the opposite. Therefore, we consider that the superior electrochemical performance of SnSe is attributed to its highly reversible conversion reaction mechanism than SnSe2.
STING Negatively Regulates Double-Stranded DNA-Activated JAK1-STAT1 Signaling via SHP-1/2 in B Cells
Yayi Hou,Guanjun Dong,Ming You,Liang Ding,Hongye Fan,Fei Liu,Deshan Ren 한국분자세포생물학회 2015 Molecules and cells Vol.38 No.5
Recognition of cytosolic DNA initiates a series of innate immune responses by inducing IFN-I production and subsequent triggering JAK1-STAT1 signaling which plays critical roles in the pathogenesis of infection, inflammation and autoimmune diseases through promoting B cell activation and antibody responses. The stimulator of interferon genes protein (STING) has been demonstrated to be a critical hub of type I IFN induction in cytosolic DNA-sensing pathways. However, it still remains unknown whether cytosolic DNA can directly activate the JAK1-STAT1 signaling or not. And the role of STING is also unclear in this response. In the present study, we found that dsDNA directly triggered the JAK1-STAT1 signaling by inducing phosphorylation of the Lyn kinase. Moreover, this response is not dependent on type I IFN receptors. Interestingly, STING could inhibit dsDNA-triggered activation of JAK1-STAT1 signaling by inducing SHP-1 and SHP-2 phosphorylation. In addition, compared with normal B cells, the expression of STING was significantly lower and the phosphorylation level of JAK1 was significantly higher in B cells from MRL/lpr lupus-prone mice, highlighting the close association between STING low-expression and JAK1-STAT1 signaling activation in B cells in autoimmune diseases. Our data provide a molecular insight into the novel role of STING in dsDNA-mediated inflammatory disorders.
Huaxin Wang,Xuan Peng,Yayi Huang,Yeda Xiao,Zhuo Wang,Liying Zhan 연세대학교의과대학 2019 Yonsei medical journal Vol.60 No.12
Purpose: The aim of this study was to investigate whether propofol could attenuate hypoxia/reoxygenation-induced apoptosisand autophagy in human renal proximal tubular cells (HK-2) by inhibiting JNK activation. Materials and Methods: HK-2 cells were treated with or without propofol or JNK inhibitor SP600125 for 1 hour and then subjectedto 15 hours of hypoxia and 2 hours of reoxygenation (H/R). Cell viability and LDH release were measured with commercial kits. Cell apoptosis was evaluated by flow cytometry. The expressions of p-JNK, cleaved-caspase-3, Bcl-2, and autophagy markers LC3and p62 were measured by Western blot or immunofluorescence. Results: HK-2 cells exposed to H/R insult showed higher cell injury (detected by increased LDH release and decreased cell viability),increased cell apoptosis index and expression of cleaved-caspase-3, a decrease in the expression of Bcl-2 accompanied byincreased expression of p-JNK and LC3II, and a decrease in expression of p62. All of these alterations were attenuated by propofoltreatment. Similar effects were provoked upon treatment with the JNK inhibitor SP600125. Moreover, the protective effects weremore obvious with the combination of propofol and SP600125. Conclusion: These results suggest that propofol could attenuate hypoxia/reoxygenation induced apoptosis and autophagy inHK-2 cells, probably through inhibiting JNK activation.
Aisyah Elliyanti,Dewi Rusnita,Nita Afriani,Yayi Dwina Billianti Susanto,Veronica Y. Susilo,Sri Setiyowati,Wirsma Arif Harahap 대한핵의학회 2020 핵의학 분자영상 Vol.54 No.1
Purpose This study investigates natrium iodide symporter (NIS) expression in three breast cancer subtypes to predict radioiodine response. Materials and Methods Frozen breast tissues from triple negative (TN), human epidermal receptor 2 (HER2+), and luminal A cancers were used in this research. NIS protein expression in each subtype was analyzed using immunohistochemistry (IHC) and western blot (WB). Secondary data such as age, subtypes, and Ki 67 index were drawn from the surgical oncologist database. Breast cancer cell lines were used to investigate the effect of radioiodine by measuring cell proliferation. Results The forty-one breast cancer samples were analyzed consisted of the following subtypes: TN, HER2+, and luminal Awere 58%, 22%, and 20%respectively. The stages of disease were 2A to 4A. Most of samples were at 3B. Ki 67 index of TN, HER2+, and luminalAwere 21 ± 12, 19 ± 5, and 7 ± 3 respectively. The NIS expressionwas detected in 95%of samples in cytoplasmand/ or cell membrane; 93% of samples were invasive breast carcinomas. Only 20% of the samples showed NIS expression at cell membrane; four samples were HER2+, and other four were TN subtypes. NIS membrane score was significantly positively correlated with Ki67 index, p = 0.04. NIS protein expression was detected at sizes 88 kDa, 50 kDa, and 27 kDa. Cell proliferation rate means of MDA-MB 231, SKBR3, and MCF7 cells were 81.6 ± 4, 10.6 ± 5, and 15.4 ± 13 respectively (p = 0.009). Conclusion NIS protein expression is detectable in breast cancer cells to varying degrees. HER2+ is the most likely to express NIS in the cell membrane followed by TN subtypes. This indicates that radioiodine could be used as a novel adjuvant treatment in breast cancer.
Kristina, Susi Ari,Endarti, Dwi,Prabandari, Yayi Suryo,Ahsan, Abdillah,Thavorncharoensap, Montarat Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.16
Background: As smoking is the leading preventable cause of multiple diseases and premature cancer deaths, estimating the burden of cancer attributable to smoking has become the standard in documenting the adverse impact of smoking. In Indonesia, there is a dearth of studies assessing the economic costs of cancers related to smoking. This study aimed to estimate indirect mortality costs of premature cancer deaths and years of potential life lost (YPLL) attributable to smoking among the Indonesian population. Materials and Methods: A prevalence based method was employed. Using national data, we estimated smoking-attributable cancer mortality in 2013. Premature mortality costs and YPLL were estimated by calculating number of cancer deaths, life expectancy, annual income, and workforce participation rate. A human capital approach was used to calculate the present value of lifetime earnings (PVLE). A discount rate of 3% was applied. Results: The study estimated that smoking attributable cancer mortality was 74,440 (30.6% of total cancer deaths), comprised of 95% deaths in men and 5% in women. Cancers attributed to smoking wereresponsible for 1,207,845 YPLL. Cancer mortality costs caused by smoking accounted for USD 1,309 million in 2013. Among all cancers, lung cancer is the leading cause of death and economic burden. Conclusions: Cancers related to smoking pose an enormous economic burden in Indonesia. Therefore, tobacco control efforts need to be prioritized in order to prevent more losses to the nation. The data of this study are important for advocating national tobacco control policy.
Impact of Smoking Cessation Training for Community Pharmacists in Indonesia
Kristina, Susi Ari,Thavorncharoensap, Montarat,Pongcharoensuk, Petcharat,Prabandari, Yayi Suryo Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.8
Background: Community pharmacists play an important role in tobacco control and adequate training on smoking cessation is essential. Materials and Methods: A quasi-experimental pre-test/post-test design was used. A one-day workshop on smoking cessation organized by Indonesian Pharmacists Association as part of PCE program was offered to 133 community pharmacists. The workshop consisted of a 3-hour lecture and a 3-hour role-play session. Pre-training and post-training surveys assessed the impact of training on parameters including knowledge, perceived role and self-efficacy with respect to smoking cessation counseling practices. Intention and ability to perform counseling using the 5A framework was assessed after training only. Results: After PCE, knowledge score significantly increased from $24.9{\pm}2.58$ to $35.7{\pm}3.54$ (p<0.001). Perceived role and self-efficacy in smoking cessation counseling also significantly increased from $25.8{\pm}2.73$ to $28.7{\pm}2.24$, and $27.6{\pm}4.44$ to $32.6{\pm}3.63$, respectively (p<0.001). After the workshop, most participants were willing to ask, advise, and assess patients who ready to quit, but were still less likely to assist in quitting plans and arranging follow up counseling. More than 75% pharmacists were able to perform cessation counseling and 65% of them can completely perform a 5A brief intervention. Conclusions: PCE can enhance pharmacists' knowledge, perceived role, self-efficacy in cessation counseling practices, and create willingness and ability to perform cessation counseling. Future training is recommended to improve skills in assisting quitting plans and arranging follow up.
Effectiveness of Tobacco Education for Pharmacy Students in Indonesia
Kristina, Susi Ari,Thavorncharoensap, Montarat,Pongcharoensuk, Petcharat,Montakantikul, Preecha,Suansanae, Thanarat,Prabandari, Yayi Suryo Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.24
Background: Smoking remains the major preventable cause of death worldwide, especially cancer-related death. Evidence clearly indicates that tobacco-related morbidity and mortality is reduced by smoking cessation. Pharmacists are well-positioned to provide tobacco cessation services an involvement of pharmacists in smoking cessation is encouraged by several organizations. While Indonesia's prevalence of smoking is in the first rank in Asian countries, none of the pharmacy schools in Indonesia are currently offering tobacco-related courses in their existing curricula at present. Our study aimed to develop and to evaluate the effectiveness of tobacco education (TE) for pharmacy students in Indonesia. Materials and Methods: A 6-hour TE was developed and evaluated using pre-test/post-test with control group design. A total of 137 fifth-year pharmacy students at Gadjah Mada University (GMU), Yogyakarta, were chosen as an intervention group while a total of 105 fifth-year students of Islamic University of Indonesia, (UII) served as the control group. Knowledge, perceived-role, self-efficacy, and ability to perform counseling using the 5A's framework were evaluated. Results: A significant improvement (P < 0.001) in knowledge, perceived-role, and self-efficacy was found in the intervention group but not in the control group. In addition, we revealed that 89.7% of the intervention group were able to perform counseling using 5A's. Conclusions: The developed TE significantly improved student knowledge, perceived-rolse, self-efficacy, and created an ability to perform cessation counseling. Integration of TE education in curricula of Indonesian pharmacy schools nation-wide should be encouraged.
STING Negatively Regulates Double-Stranded DNA-Activated JAK1-STAT1 Signaling via SHP-1/2 in B Cells
Dong, Guanjun,You, Ming,Ding, Liang,Fan, Hongye,Liu, Fei,Ren, Deshan,Hou, Yayi Korean Society for Molecular and Cellular Biology 2015 Molecules and cells Vol.38 No.5
Recognition of cytosolic DNA initiates a series of innate immune responses by inducing IFN-I production and subsequent triggering JAK1-STAT1 signaling which plays critical roles in the pathogenesis of infection, inflammation and autoimmune diseases through promoting B cell activation and antibody responses. The stimulator of interferon genes protein (STING) has been demonstrated to be a critical hub of type I IFN induction in cytosolic DNA-sensing pathways. However, it still remains unknown whether cytosolic DNA can directly activate the JAK1-STAT1 signaling or not. And the role of STING is also unclear in this response. In the present study, we found that dsDNA directly triggered the JAK1-STAT1 signaling by inducing phosphorylation of the Lyn kinase. Moreover, this response is not dependent on type I IFN receptors. Interestingly, STING could inhibit dsDNA-triggered activation of JAK1-STAT1 signaling by inducing SHP-1 and SHP-2 phosphorylation. In addition, compared with normal B cells, the expression of STING was significantly lower and the phosphorylation level of JAK1 was significantly higher in B cells from MRL/lpr lupus-prone mice, highlighting the close association between STING low-expression and JAK1-STAT1 signaling activation in B cells in autoimmune diseases. Our data provide a molecular insight into the novel role of STING in dsDNA-mediated inflammatory disorders.