Total Flavonoid Extract from Abelmoschus manihot (L.) Medic Flowers Attenuates d-Galactose-Induced Oxidative Stress in Mouse Liver Through the Nrf2 Pathway

Yan Qiu,Peng-Fei Ai,Jian-Jun Song,Chang Liu,Zhi-Wei Li 한국식품영양과학회 2017 Journal of medicinal food Vol.20 No.6

Abelmoschus manihot (L.) Medic is an edible hibiscus that is rich in flavonoids, and its use as Chinese herbal medicine for the treatment of diseases and health maintenance dates back to ancient times. The chemical compositions of total flavonoid of A. manihot (L.) Medic flower extract (TFAE) were identified and determined by high performance liquid chromatography (HPLC). The effects of TFAE on antioxidative activities in a d-galactose (d-gal)-induced mouse model and Nrf2-mediated antioxidant responses were evaluated. Male Kunming mice were randomly divided into normal control group, d-gal aging model group, d-gal+ascorbic acid group that served as a positive control, and d-gal+TFAE (40, 80, and 160 mg TFAE/kg) group. After 42 days, the antioxidant effects of these treatments were determined by biochemical studies, Western blotting, quantitative real-time polymerase chain reaction, and histological analysis. The results showed that the groups administered TFAE exhibited significant elevation in liver activities of antioxidant enzymes, including catalase (CAT), glutathione peroxidase (GPx), superoxide dismutase (SOD), and total antioxidant capacity (T-AOC), and decreased malondialdehyde (MDA) production in a dose-dependent manner compared with the d-gal-induced model group. Expression of Nrf2 and its target antioxidants (HO-1 and NQO1) was manifestly increased by TFAE treatment. TFAE also increased mRNA expression of GPx, SOD, and CAT and decreased tumor necrosis factor-alpha (TNF-α) and interleukin-1 beta (IL-1β). Furthermore, the microstructure of livers in TFAE-administered mice was obviously improved as compared with the d-gal model group. These results suggest that TFAE protects mice against d-gal-induced oxidative stress, and the effect is related to the activation of Nrf2 signaling.

The Expression of Aldehyde Dehydrogenase Family in Breast Cancer

Yan Qiu,Tianjie Pu,Li Li,Fei Cheng,Changli Lu,Linyong Sun,Xiao Teng,Feng Ye,Hong Bu 한국유방암학회 2014 Journal of breast cancer Vol.17 No.1

Purpose: It is widely accepted that aldehyde dehydrogenase(ALDH) activity is a signature of breast cancer stem cells, andhigh activity has been reported to be associated with poor clinicaloutcome. The aim of this study was to assess the expressionof members of the ALDH family of isozymes in breast cancer tissuesand to evaluate the implications of the results. Methods: Weanalyzed paraffin-embedded tumor tissue from 160 patients withbreast cancer. Immunohistochemistry (IHC) staining was performedon the slides using antibodies against different ALDHfamily members. We collated the IHC results with patient clinicalcharacteristics and determined their prognostic value. In addition,we analyzed normal, hyperplastic, and carcinomatous tissues insitu to check their ALDH distributions. Results: All the testedALDH members were detected in the various tissue types, but atdifferent levels. Only ALDH 1A3 was found to be significantly associatedwith distant metastasis (p=0.001), disease-free survival(p<0.001), and overall survival (p<0.001). Conclusion: The levelof ALDH 1A3 in breast cancer tissue is a predictive marker of apoor clinical outcome.

Influence of heat treatment on complex-shape rotating disk subjected to isothermal precision forging

Yanqiu Zhang,Shuyong Jiang,Xiaoming Zhu,Yanan Zhao,Debin Shan 대한기계학회 2017 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.31 No.1

Influence of heat treatment on the mechanical properties of the forging was systematically investigated according to tensile test, hardness test and electrical conductivity test. Simultaneously, typical microstructures of the 7A09 aluminum alloy samples subjected to various solution temperatures and aging regimes were characterized by means of Transmission electron microscope (TEM) and Scanning electron microscope (SEM). In the case of the different solution temperatures, the size of precipitations increases with the increase in the solution temperature, which contributes to improving both strength and stress corrosion resistance of the forging. The solution temperature of 465 °C leads to the well comprehensive mechanical properties and the superior stress corrosion resistance of the forging. G.P. zone, η' phase and η phase are able to precipitate in the matrix of the forging in the case of different aging regimes, such as peak aging, two-stage overaging, two-stage aging, and regression and re-aging. The precipitation phases play an important role in enhancing the mechanical properties of the forging as an obstacle against the dislocation movement.

A two-step blind source extraction method and its application in fault diagnosis of rolling element bearing

YanQiu Xiao,Hongchao Wang 대한기계학회 2019 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.33 No.3

The vibration signals will take on cyclical characteristics when fault arises in the rolling element bearing, and a two-step blind source extraction (BSE) method for fault diagnosis of rolling element bearing is proposed in the paper using the above property. Firstly, calculate the theoretical basic cyclet of the target source fault signal, and the weighted separation matrixand desired source signal are obtained coarsely. Secondly, useas the initial weighted matrix and apply the fixed-point algorithm basing on high-order statistics on the observed signals, and much more perfect target source signal is got at last. The proposed method has the following advantages over other BSE method such as constrained independent component analysis (CICA) basing on the analyzed results of simulation and experiment: The fundamental period t of the target source signal does not needed to be estimated accurately, and the reference signal also does not need to be constructed precisely. However, these two conditions are the necessary prerequisites of CICA. Besides, the proposed method also has the advantage of higher accuracy over the other recent BSE methods through comparison.

A Study on Limitation of Liability for Maritime Claims under the Maritime Code of China

Yanqiu Ma 한국해사법학회 2003 해사법연구 Vol.15 No.2

In regard to limitation of liability for maritime claims, there are the 1976 Convention and the Athens Convention. Many maritime countries have drafted their own provisions for such limitation of liability. The relevant provisions under the Maritime Code of China are quite different from those of other maritime countries. The purpose of this article is to make a study on the basic interpretation of the system of limitation of liability for maritime claims under the Maritime Code of China and to explore ways to remedy the defects existing in the Chinese law.

A rapid and quantitative fluorescent microsphere immunochromatographic strip test for detection of antibodies to porcine reproductive and respiratory syndrome virus

Yanqiu Wei,Baozhi Yang,Yunlong Li,Yongcheng Duan,Deyu Tian,Baoxiang He,Chuangfu Chen,Wenjun Liu,Limin Yang 대한수의학회 2020 Journal of Veterinary Science Vol.21 No.4

A fluorescent microsphere-based immunochromatographic strip test (FICT) was developed for the rapid, sensitive, and quantitative detection of porcine reproductive and respiratory syndrome virus (PRRSV) antibodies at the pen-side. The assay was based on the formation of a sandwich immune-complex (anti-pig IgG-PRRSV antibodies-NSP7/N), which was validated by a comparison with IDEXX-ELISA using 3325 clinical specimens. The diagnostic specificity, sensitivity, and accuracy of FICT were 97.28, 93.41, and 94.95%, respectively. FICT showed a good correlation with the virus neutralization assay. Overall, a promising pen-side diagnostic tool was developed for the rapid and quantitative detection of PRRSV antibodies within 15 min.

Imaging Manifestations and Misdiagnosis Analysis of Six Cases of Bone Hydatid Disease

Yanqiu Sun,Chunlong Yan,Dengfeng Tian,Chenhong Zhang,Qiang Zhang 대한기생충학ㆍ열대의학회 2022 The Korean Journal of Parasitology Vol.60 No.6

We retrospectively evaluated the clinical and imaging features of 6 patients with bone hydatid disease confirmed by surgery and pathological examination. Among the 6 patients, 2 were infected with Echinococcosis granulosus metacestode and 4 were infected with E. multilocularis metacestode. The 2 cases with cystic echinococcosis were diagnosed by computed tomographic (CT) examination, and other 4 cases were diagnosed by magnetic resonance (MR) imaging. On the initial evaluation, 1 case each was misdiagnosed as a giant cell tumor or neurogenic tumor, and 2 were misdiagnosed as tuberculosis. The imaging manifestations of bone hydatid disease are complex, but most common findings include expansive osteolytic bone destruction, which may be associated with sclerosing edges or dead bone formation, localized soft tissue masses, and vertebral lesions with wedge-shaped changes and spinal stenosis. Combining imaging findings with the patient’s epidemiological history and immunological examinations is of great help in improving the diagnosis and differential diagnosis of bone hydatid disease.

The Current Situation of Internet Addiction and Its Impact on Sleep Quality and Self-Injury Behavior in Chinese Medical Students

Yanqiu Wang,Ying Zhao,Ling Liu,Yan Chen,Dong Ai,Yingshui Yao,Yuelong Jin 대한신경정신의학회 2020 PSYCHIATRY INVESTIGATION Vol.17 No.3

Objective The purpose of this cross-sectional survey is to explore the current state of Internet addiction (IA) in Chinese medical students and its connection with medical students’ sleep quality and self-injury behavior. Methods Respondents were came from Wannan Medical College, China. The Young’s Internet Addiction Test, Pittsburgh Sleep Quality Index (PSQI), Self-Harm Questionnaire were used in this cross-sectional survey. A total of 3,738 medical students were investigated, 1,552 (41.52%) males, 2,186 (58.48%) females. T-test, chi-square test and MANOVA were used for data analysis. Results Of the 3,738 medical students, 1,054 (28.2%) reported having IA, 1,126 (30.1%) reported having poor sleep quality, 563 (15.1%) having self-harm behaviors. IA tends to be more female, upper grade students. The sleep quality of IA was worse than that of non-IA (χ2=54.882, p<0.001), and the possibility of self-injury was higher than non-IA (χ2=107.990, p<0.001). Conclusion This survey shows that the IA detection rate of medical students was 28.2%. Females, higher grade students had a higher IA detection rate. The low sleep quality and self-injury behavior of medical students are associated with IA.

Research on the Microstructure Characteristic and Tensile Property of Laser‑MIG Hybrid Welded Joint for 5A06 Aluminum Alloy

Yanqiu Zhao,Xiaohong Zhan,Qiyu Gao,Shuai Chen,Yue Kang 대한금속·재료학회 2020 METALS AND MATERIALS International Vol.26 No.3

Laser-metal inter gas (MIG) hybrid welding technique provides higher overall productivity over the traditional fusionwelding processes for joining medium-thick aluminum alloy. In this paper, laser-MIG hybrid welding experiment was performedby the TruDisk 12003 Laser and KUKA robot. The second fusion line, the laser-dominated region and laser-MIGhybrid-dominated region were introduced to this study to discuss the microstructure distribution. A three-dimensionalfinite element model was established by MSC. Marc to study the thermal field distribution of laser-MIG hybrid weldingprocess. It found that the temperature in the laser-MIG hybrid-dominated region was much higher than the laser-dominatedregion. The formation of the second fusion line in the welded joint is mainly owing to the different heat effect of laser-archybrid-dominated region and laser-dominated region. The crystalline size of equiaxed dendrite grains in the laser-MIGhybrid-dominated region is finer than the laser-dominated region. In addition, the tensile property was measured to dissectthe failure mechanism of the laser-MIG hybrid welding joints. The element content in fractured surface at the position ofdimple and pore wall were comprehensively analyzed. The element content of Mg and O in pore wall are higher than dimple. In the solidification process, the growth of equiaxed dendrite grains is in part hindered by the existence of coarse pores inthe laser-MIG hybrid-dominated region.

Strain-Rate Effect on the Stress and Strain Concentration in a Visco-Plastic Plate With An Elliptic Hole

Yanqiu Chen,Jin Zhang,Peishi Yu,Jian Wu,Weibo Wang,Junhua Zhao 한국강구조학회 2020 International Journal of Steel Structures Vol.20 No.4

The stress and strain concentration in some component has been a signifi cant topic for structural safety assessment. However, the evaluation of the stress and strain concentration based on the widely existing solutions for linear elastic material under static loading would lead to remarkable errors when the material undergoes the dynamic loading, since the coupling eff ect induced by the nonlinearity of the stress–strain relationship and the strain-rate strengthening could be of signifi cant infl uence. Here we analyzed the strain-rate dependent concentration of stress and strain for a visco-plastic plate with an elliptic hole subjected to dynamic loading. The three-dimensional stress and strain are calculated by fi nite element analyses based on a rate-dependent Johnson–Cook model in which the material constants are set according to a typical visco-plastic standard 45 carbon steel. Our results show that both the plastic stress and strain concentration factors signifi cantly depend on not only the strain but also the strain rate. With the increment of the remote strain rate, the stress concentration monotonically increases, while the strain concentration shows decreasing tendency.