A New Eccentric Annular Leakage Model for Rod Pump with Couette-Poiseuille Flow

Yanqiang Wu,Xiaodong Wu,Yining Wang,Yu Yuan 보안공학연구지원센터 2013 International Journal of Control and Automation Vol.6 No.6

Study and Design a Dynamic E-business Application Framework Based on Java EE and Web Service

Yanqiang Hu 보안공학연구지원센터 2016 International Journal of Database Theory and Appli Vol.9 No.11

With the rapid development of Internet and information technologies, E-business is rapidly becoming the focus of business activity. However the traditional E-business application system exists the non-unitary technical standards, lack of unified commercial release mechanism, difficult information exchange and cooperation, long development time, difficult reconstruction and upgrading maintenance. A large number of Web services, JAVA EE technology has become more mature and stable. So the key techniques of XML, SOAP, WSDL and UDDI in Web service are analyzed in detail, then a new E-business application framework based on Java EE and Web services is proposed to overcome the shortcomings of the traditional E-business application system. In the this E-business application framework, the characteristics and architecture of Web service are used to realize the standard and loosely coupled application architecture and guarantee compatible information exchange and cooperation. The Java EE framework is use to ensure the more strong security and better stability for E-business application framework. So the new E-business application framework takes on crossing platform, flexibility and easy expansion, and can meet the openness, complexity, distribution, dynamic and customization of E-business.

Research on Cloud Safe Storage Evaluation Model from Perspective of Internet Finance

Hu yanqiang 보안공학연구지원센터 2016 International Journal of Grid and Distributed Comp Vol.9 No.7

A hybrid variable selection algorithm for multi-layer perceptron with nonnegative garrote and extremal optimization

Xiuliang Wu,Yanqiang Li,Hao Wu,Fangfang Zhang,Kai Sun 제어로봇시스템학회 2019 제어로봇시스템학회 국제학술대회 논문집 Vol.2019 No.10

In the paper, a new hybrid variable selection algorithm for nonlinear regression multi-layer perceptron (MLP) is proposed. The proposed algorithm applies nonnegative garrote (NNG) to compress the input weights of the MLP. The zero input weights dependent variables will be removed from the initial dataset. Next, a further variable selection is carried out by extremal optimization (EO) algorithm. The new variable selection algorithm integrates powerful global selection ability of NNG and accurate local search ability of EO. Finally, two examples of artificial data sets and an industrial application for a debutanizer column are implemented to demonstrate the performance of the new algorithm. The simulation result demonstrates that the developed algorithm presents d better model performance along with less input variable selected than other state-of-art variable selection methods.

Improved Stability of Aluminum Foam Through Heat Treatment of Foamable Precursor

Fan Deng,Yanqiang Liu,Xuehan Lu,Jianzhong Fan 대한금속·재료학회 2020 METALS AND MATERIALS International Vol.26 No.10

Foam stability, characterized by the dynamics of foam expansion and decay, is essential for producing aluminum foam. While, the stability is often difficult to control. In present study, the heat treatment of foamable precursor was applied toimprove the foam stability via the powder route. Interestingly, both process of foam expansion and decay were remarkablyslow down after heat treatment. The time to achieve peak expansion was prolonged to 2.38 times, and the time span frompeak expansion to obvious drainage and collapse happening increased to 6 times. The enhanced stability is thought to relateto the oxide-flakes on the cell face caused by heat treatment.

Role of Reactive Oxygen Species in TGF-β1-Induced Mitogen-Activated Protein Kinase Activation and Epithelial-Mesenchymal Transition in Renal Tubular Epithelial Cells

Rhyu, Dong-Young,Yang, Yanqiang,Ha, Hun-Joo,Lee, Geun-Taek,Song, Jae-Sook,Uh, Soo-Taek,Lee, Hi-Bahl 이화여자대학교 약학연구소 2004 藥學硏究論文集 Vol.- No.14

Epithelial-mesenchymal transition (EMT) plays an important role in renal tubulointerstitial fibrosis and TGF-β1 is the key inducer of EMT. Phosphorylation of Smad proteins and/or mitogen-activated protein kinases (MAPK) is required for TGF-β1-induced EMT. Because reactive oxygen species (ROS) are involved in TGF-β1 signaling and are upstream signaling molecules to MAPK, this study examined the role of ROS in TGF-β1-induced MAPK activation and EMT in rat proximal tubular epithelial cells. Growth-arrested and synchronized NRK-52E cells were stimulated with TGF-β1 (0.2 to 20 ng/ml) or H₂0₂ (1 to 500 μM) in the presence or absence of antioxidants (N-acetylcysteine or catalase), inhibitors of NADPH oxidase (diphenyleneiodonium and apocynin), mitochondrial electron transfer chain subunit I (rotenone), and MAPK (PD 98059, an MEK [MAP kinase/ERK kinase] inhibitor, or p38 MAPK inhibitor) for up to 96 h. TGF-β1 increased dichlorofluorescein-sensitive cellular ROS, phosphorylated Smad 2, p38 MAPK, extracellular signal-regulated kinases (ERK)1/2, α-smooth muscle actin (α-SMA) expression, and fibronectin secretion and decreased E-cadherin expression. Antioxidants effectively inhibited TGF-β1-induced cellular ROS, phosphorylation of Smad 2, p38 MAPK, and ERK, and EMT. H₂0₂ reproduced all of the effects of TGF-β1 with the exception of Smad 2 phosphorylation. Chemical inhibition of ERK but not p38 MAPK inhibited TGF-131-induced Smad 2 phosphorylation, and both MAPK inhibitors inhibited TGF-β1- and H₂0₂-induced EMT. Diphe-nyleneiodonium, apocynin, and rotenone also significantly inhibited TGF-β1-induced ROS. Thus, this data suggest that ROS play an important role in TGF-β1-induced EMT primarily through activation of MAPK and subsequently through ERK-directed activation of Smad pathway in proximal tubular epithelial cells.

Role of Reactive Oxygen Species in TGF-β1-Induced Mitogen-Activated Protein Kinase Activation and Epithelial-Mesenchymal Transition in Renal Tubular Epithelial Cells

Rhyu, Dong-Young,Yang, Yanqiang,Ha, Hun-Joo,Lee, Geun-Taek,Song, Jae-Sook,Uh, Soo-Taek,Lee, Hi-Bahl 이화여자대학교 약학연구소 2005 藥學硏究論文集 Vol.- No.16

Epithelial-mesenchymal transition (EMT) plays an important role in renal tubulointerstitial fibrosis and TGF-β1 is the key inducer of EMT. Phosphorylation of Smad proteins and/or mitogen-activated protein kinases (MAPK) is required for TCP-β1-induced EMT. Because reactive oxygen species (ROS) are involved in TCF-β1 signaling and are upstream signaling molecules to MAPK, this study examined the role of ROS in TGF-β1-induced MAPK activation and EMT in rat proximal tubular epithelial cells. Growth-arrested and synchronized NRK-52E cells were stimulated with TGF-β1 (0.2 to 20 ng/ml) or H_(2)O_(2) (1 to 500 ㎛) in the presence or absence of antioxidants (N-acetylcysteine or catalase), inhibitors of NADPH oxidase (diphenyleneiodonium and apocynin), mitochondrial electron transfer chain subunit I (rotenone), and MAPK (PD 98059, an MEK [MAP kinase/ERK kinase] inhibitor, or p38 MAPK inhibitor) for up to 96 h. TGF-β1 increased dichlorofluoresceinsensitive cellular ROS, phosphorylated Smad 2, p38 MAPK, extracellular signal-regulated kinases (ERK) 1/2, α-smooth muscle actin (α-SMA) expression, and fibronectin secretion and decreased I-cadherin expression. Antioxidants effectively inhibited TGF-β1-induced cellular ROS, phosphorylation of Smad 2, p38 MAPK, and ERK, and EMT. H_(2)O_(2) reproduced all of the effects of TGF-β1 with the exception of Smad 2 phosphorylation. CHemical inhibition of ERK but not p38 MAPK inhitited TGF-91-induced Smad 2 phosphorylation, and both MAPK inhibitors inhibited TGF-β1- and H_(2)O_(2)-induced EMT. Diphe-nyleneiodonium, apocynin, and rotenone also significantly inhibited TGF-β1-indured ROS. Thus, this data suggest that ROSplay an important role in TGF-β1-induced EMT primarily through activation of MAPK and subsequently through ERK-directed activation of Smad pathway in proximal tubular epithelial cells.

Role of Reactive Oxygen Species in TGF-β1-Induced Mitogen-Activated Protein Kinase Activation and Epithelial-Mesenchymal Transition in Renal Tubular Epithelial Cells

Rhyu, Dong Young,Yang, Yanqiang,Ha, Hunjoo,Lee, Geun Taek,Song, Jae Sook,Uh, Soo-taek,Lee, Hi Bahl American Society of Nephrology 2005 Journal of the American Society of Nephrology Vol.16 No.3

<P>Epithelial-mesenchymal transition (EMT) plays an important role in renal tubulointerstitial fibrosis and TGF-beta1 is the key inducer of EMT. Phosphorylation of Smad proteins and/or mitogen-activated protein kinases (MAPK) is required for TGF-beta1-induced EMT. Because reactive oxygen species (ROS) are involved in TGF-beta1 signaling and are upstream signaling molecules to MAPK, this study examined the role of ROS in TGF-beta1-induced MAPK activation and EMT in rat proximal tubular epithelial cells. Growth-arrested and synchronized NRK-52E cells were stimulated with TGF-beta1 (0.2 to 20 ng/ml) or H(2)O(2) (1 to 500 microM) in the presence or absence of antioxidants (N-acetylcysteine or catalase), inhibitors of NADPH oxidase (diphenyleneiodonium and apocynin), mitochondrial electron transfer chain subunit I (rotenone), and MAPK (PD 98059, an MEK [MAP kinase/ERK kinase] inhibitor, or p38 MAPK inhibitor) for up to 96 h. TGF-beta1 increased dichlorofluorescein-sensitive cellular ROS, phosphorylated Smad 2, p38 MAPK, extracellular signal-regulated kinases (ERK)1/2, alpha-smooth muscle actin (alpha-SMA) expression, and fibronectin secretion and decreased E-cadherin expression. Antioxidants effectively inhibited TGF-beta1-induced cellular ROS, phosphorylation of Smad 2, p38 MAPK, and ERK, and EMT. H(2)O(2) reproduced all of the effects of TGF-beta1 with the exception of Smad 2 phosphorylation. Chemical inhibition of ERK but not p38 MAPK inhibited TGF-beta1-induced Smad 2 phosphorylation, and both MAPK inhibitors inhibited TGF-beta1- and H(2)O(2)-induced EMT. Diphenyleneiodonium, apocynin, and rotenone also significantly inhibited TGF-beta1-induced ROS. Thus, this data suggest that ROS play an important role in TGF-beta1-induced EMT primarily through activation of MAPK and subsequently through ERK-directed activation of Smad pathway in proximal tubular epithelial cells.</P>

Effect of Cu on Pore Structure of Al–Si Foam

Fan Deng,Jianzhong Fan,Yanqiang Liu,Xuehan Lu,Shaohua Wei,Junhui Nie 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.12

The alloy composition is an important factor effecting the pore structure of aluminum foam. In this paper, the powder compactfoaming method was used, and different content of Cu element powders were added to the alloy Al–9Si, for investigatingthe effect of alloying element on foaming process. With the Cu content of Al–Si–Cu alloy increasing, the expansion ratio isincreased, the time required to reach maximum expansion is shortened, and the number of fissure-like pores at early foamingstage is decreased. This improving action is related to the higher volume fraction of liquid phase in same melting temperaturecaused by increasing Cu content in alloy. Comparing different Al–Si–Cu alloys with different Cu content at same expansionratio, the higher Cu content, the smaller pore size and the thinner cell wall. This beneficial effect on pore structure is attributedto the addition of Cu that is benefit to increasing the viscosity of Al–Si–Cu alloy during foaming. And at same expansionrate, the more content of Cu in Al–Si–Cu alloy, the higher compressive strength of aluminum foam.

MiR-124 regulates apoptosis in hypoxia-induced human brain microvessel endothelial cells through targeting Bim

Jie Pu,Yuan Long,Jian Zhou,Yanqiang Zhan,Xiaoyong Qin 한국응용생명화학회 2018 Applied Biological Chemistry (Appl Biol Chem) Vol.61 No.6

Human brain microvessel endothelial cells (HBMECs) are crucial for brain vascular repair and maintenance. The high-expressed expressions of micro- RNA-124 (miR-124) in brain have been investigated and revealed in many researches. In this work, we aimed to investigate the role of miR-124 in apoptosis of HBMECs and the underlying mechanism. Here, we found the lowexpressed miR-124 in hypoxia-induced HBMECs using qRT-PCR analysis. MiR-124 targeting 30-untranslated region (30-UTR) of Bim mRNA was predicted by Targetscan database. Importantly, the decreased miR-124 expression and increased Bim expression, an opposite trend, were obtained in hypoxia-induced HBMECs. The further confirmation of the correlation between miR-124 and Bim was conducted by miR-124 overexpression and dual luciferase reporter assays. The inhibitory role of miR- 124 in Bim expression was evidenced by results obtained from miR-124 overexpression analysis. Luciferase reporter assay further proved that miR-124 directly targeted the two conserved seed sites in the Bim 30-UTR. The inhibited apoptosis of HBMECs was observed under both miR-124 overexpression and Bim knockdown condition in flow cytometry analysis. Collectively, these findings outline that miR-124 regulates apoptosis in hypoxia-induced HBMECs through targeting Bim, providing a better understanding of the role of miR-124 in apoptosis of HBMECs.