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Yang Sheng,Xie JiaJun,Pan ZhiJie,Guan HongMei,Tu YueSheng,Ye YuanJian,Huang ShouBin,Fu ShiQiang,Li KangXian,Huang ZhiWei,Li XiaoQi,Shi ZhanJun,Li Le,Zhang Yang 생화학분자생물학회 2024 Experimental and molecular medicine Vol.56 No.-
The meniscus is vital for maintaining knee homeostasis and function. Meniscal calcification is one of the earliest radiological indicators of knee osteoarthritis (KOA), and meniscal calcification is associated with alterations in biomechanical properties. Meniscal calcification originates from a biochemical process similar to vascular calcification. Advanced glycation end products (AGEs) and their receptors (RAGEs) reportedly play critical roles in vascular calcification. Herein, we investigated whether targeting AGE-RAGE is a potential treatment for meniscal calcification. In our study, we demonstrated that AGE-RAGE promotes the osteogenesis of meniscal cells and exacerbates meniscal calcification. Mechanistically, AGE-RAGE activates mTOR and simultaneously promotes ATF4 accumulation, thereby facilitating the ATF4-mTOR positive feedback loop that enhances the osteogenic capacity of meniscal cells. In this regard, mTOR inhibits ATF4 degradation by reducing its ubiquitination, while ATF4 activates mTOR by increasing arginine uptake. Our findings substantiate the unique role of AGE-RAGE in the meniscus and reveal the role of the ATF4-mTOR positive feedback loop during the osteogenesis of meniscal cells; these results provide potential therapeutic targets for KOA.
Transcriptome profile of one-month-old lambs' granulosa cells after superstimulation
Wu, Yangsheng,Lin, Jiapeng,Li, Xiaolin,Han, Bing,Wang, Liqin,Liu, Mingjun,Huang, Juncheng Asian Australasian Association of Animal Productio 2017 Animal Bioscience Vol.30 No.1
Objective: Superstimulatory treatment of one-month-old lambs can achieve synchronous development of numerous growing follicles. However, these growing follicles cannot complete maturation and ovulation. Oocyte maturation and competence are acquired during follicular development, in which granulosa cells play an essential role. Methods: In this study, we applied RNA sequencing to analyze and compare gene expression between prepubertal and adult superstimulated follicle granulosa cells in sheep. Results: There were more than 300 genes that significantly differed in expression. Among these differently expressed genes, many extracellular matrix genes (EGF containing Fibulin Like Extracellular Matrix Protein 1, pentraxin 3, adrenomedullin, and osteopontin) were significantly down-regulated in the superstimulated follicles. Ingenuity pathway and gene ontology analyses revealed that processes of axonal guidance, cell proliferation and DNA replication were expressed at higher levels in the prepubertal follicles. Epidermal growth factor, T-Box protein 2 and beta-estradiol upstream regulator were predicted to be active in prepubertal follicles. By comparison, tumor protein P53 and let-7 were most active in adult follicles. Conclusion: These results may contribute to a better understanding of the mechanisms governing the development of granulosa cells in the growing follicle in prepubertal sheep.
Global Minimum-Jerk Trajectory Planning of Space Manipulator
Panfeng Huang,Yangsheng Xu,Bin Liang 대한전기학회 2006 International Journal of Control, Automation, and Vol.4 No.4
A novel approach based on genetic algorithms (GA) is developed to find a global minimum-jerk trajectory of a space robotic manipulator in joint space. The jerk, the third derivative of position of desired joint trajectory, adversely affects the efficiency of the control algorithms and stabilization of whole space robot system and therefore should be minimized. On the other hand, the importance of minimizing the jerk is to reduce the vibrations of manipulator. In this formulation, a global genetic-approach determines the trajectory by minimizing the maximum jerk in joint space. The planning procedure is performed with respect to all constraints, such as joint angle constraints, joint velocity constraints, joint angular acceleration and torque constraints, and so on. We use an genetic algorithm to search the optimal joint inter-knot parameters in order to realize the minimum jerk. These joint inter-knot parameters mainly include joint angle and joint angular velocities. The simulation result shows that GA-based minimum-jerk trajectory planning method has satisfactory performance and real significance in engineering.
Jiaqiang Yang,Yongle Mao,Yangsheng Chen 대한전기학회 2017 Journal of Electrical Engineering & Technology Vol.12 No.3
Estimation errors of the rotor speed and position in sensorless control systems of Permanent Magnet Synchronous Motors (PMSM) will lead to low efficiency and dynamic-performance degradation. In this paper, a parallel-type extended nonlinear observer incorporating the nominal parameters is constructed in the stator-fixed reference frame, with rotor position, speed, and the load torque simultaneously estimated. The stability of the extended nonlinear observer is analyzed using the indirect Lyapunov’s method, and observer gains are selected according to the transfer functions of the speed and position estimators. Taking into account the parameter inaccuracies issue, explicit estimation error equations are derived based on the error dynamics of the closed-loop sensorless control system. An equivalent flux error is defined to represent the back Electromotive Force (EMF) error caused by the inaccurate motor parameters, and a compensation strategy is designed to suppress the estimation errors. The effectiveness of the proposed method has been validated through simulation and experimental results.
A Generalized Padé Approximation of Time Delay Operator
Yong Wang,Yiheng Wei,Yangsheng Hu,Yi Dai 제어·로봇·시스템학회 2016 International Journal of Control, Automation, and Vol.14 No.1
This paper addresses the problem of fractional order Padé approximation for the time delay operator. Based on optimization method, a fitting polynomial with basic order a is constructed to approximate the time delayoperator. This makes for two generalized Padé approximations in the form of normal and symmetric approximation,respectively. The advantage of the proposed method lies in the fact that the resulting approximate model is inthe standard transform function form of any commensurate order a, making all of the existing stability analysismethods and control design tools for such systems amenable to the fractional order time delay systems. Somenumerical examples are given to demonstrate the effectiveness of the proposed method.
Huaiyang Xiong,Jing Yang,Yangsheng Li 한국식물학회 2012 Journal of Plant Biology Vol.55 No.3
It has been known that SUB1A-1 confers submergence tolerance to rice (Oryza sativa L.). However, two indica rice genotypes, FR13A and Goda Heenati both carrying this gene, exhibited differential tolerance to submergence. Using oligonucleotide microarray combined with suppression subtractive hybridization, we identified a different number of submergence-responsive genes in each genotype. Two-way analysis of variance (ANOVA) of microarray data indicated that there was a high degree of congruency between transcriptional profiles of the two genotypes in response to submergence. Only two genes exhibited an opposing regulation pattern between genotypes after submergence and 324 genes were regulated by submergence only in one genotype and unchanged in their expression in the other, suggesting differential submergence responses. These genes were potentail candidates responsible for differential tolerance observed between genotypes. Gene ontology analysis demonstrated that they were significantly enriched in the processes involved in defense response and flavonoid biosynthesis. The current study provides novel clues for further understanding of the mechanism of submergence tolerance.
Knockout of Myostatin by Zinc-finger Nuclease in Sheep Fibroblasts and Embryos
Zhang, Xuemei,Wang, Liqin,Wu, Yangsheng,Li, Wenrong,An, Jing,Zhang, Fuchun,Liu, Mingjun Asian Australasian Association of Animal Productio 2016 Animal Bioscience Vol.29 No.10
Myostatin (MSTN) can negatively regulate the growth and development of skeletal muscle, and natural mutations can cause "double-muscling" trait in animals. In order to block the inhibiting effect of MSTN on muscle growth, we transferred zinc-finger nucleases (ZFN) which targeted sheep MSTN gene into cultured fibroblasts. Gene targeted colonies were isolated from transfected fibroblasts by serial dilution culture and screened by sequencing. Two colonies were identified with mono-allele mutation and one colony with bi-allelic deletion. Further, we introduced the MSTN-ZFN mRNA into sheep embryos by microinjection. Thirteen of thirty-seven parthenogenetic embryos were targeted by ZFN, with the efficiency of 35%. Our work established the technical foundation for generation of MSTN gene editing sheep by somatic cloning and microinjection ZFN into embryos.
Yongle Mao,Jiaqiang Yang,Dejun Yin,Yangsheng Chen 전력전자학회 2016 JOURNAL OF POWER ELECTRONICS Vol.16 No.6
Mechanical and electrical parameter uncertainties cause dynamic and static estimation errors of the rotor speed and position, resulting in performance deterioration of sensorless control systems. This paper applies an extended nonlinear observer to interior permanent magnet synchronous motors (IPMSM) for the simultaneous estimation of the rotor speed and position. Two compensation methods are proposed to improve the observer performance against parameter uncertainties: an on-line rotational inertia adjustment approach that employs the gradient descent algorithm to suppress dynamic estimation errors, and an equivalent flux error compensation approach to eliminate static estimation errors caused by inaccurate electrical parameters. The effectiveness of the proposed control strategy is demonstrated by experimental tests.