Quantitative Assay for the Binding of Jun-Fos Dimer and Activator Protein-1 Site

Yang,Chul-Hak,Jun,Gyo,Hahm,Eun-Ryeong,Lee,Sangkyou,Park,Seyeon,Lee,Dug-Keun The Korea Science and Technology Center 1999 BMB Reports Vol.32 No.6

The Jun and Fos families of eukaryotic transcription factors form heterodimers capable of binding to their cognate DNA enhancer elements. We are interested in searching for inhibitors or antagonists of the binding of the Jun-Fos heterodimer to the activator protein-1 (AP-1) site. The basic-region leucine zipper (bZIP) domain of c-Fos was expressed as a fusion protein with glutathione S-transferase, and allowed to form a heterodimer with the bZIP domain of c-Jun. The heterodimer was bound to glutathione-agarose, to which were added radiolabeled AP-1 uncleotides. After thorough washing, the gel-bound radioactivity was counted. The assay is faster than the coventional electrophoretic mobility shift assay because the gel electrophoresis step and the autoradiography step are eliminated. Moreover, the assay is very sensitive, allowing the detection of picomolar quantities of nucleotides, and is not affected by up to 50% dimethylsulfoxide, a solvent for hydrophobic inhibitors. Curcumin and dihydroguaiaretic acid, recently known inhibitors of Jun-Fos-DNA complex formation, were applied to this Jun-GST-fused Fos system and revealed to decrease the dimer-DNA binding.

Quantitative Assay for the Binding of Jun-Fos Dimer and Activator Protein-1 Site

Yang, Chul Hak,Jun, Gyo,Lee, Dug Keun,Lee, Sang Kyou,Park, Se Yeon,Hahm, Eun Ryeong 생화학분자생물학회 1998 BMB Reports Vol.32 No.6

The Jun and Fos families of eukaryotic transcription factors form heterodimers capable of binding to their cognate DNA enhancer elements. We are interested in searching for inhibitors or antagonists of the binding of the Jun-Fos heterodimer to the activator protein-1 (AP-1) site. The basic-region leucine zipper (bZIP) domain of c-Fos was expressed as a fusion protein with glutathione S-transferase, and allowed to form a heterodimer with the bZIP domain of c-Jun. The heterodimer was bound to glutathione-agarose, to which were added radiolabeled AP-1 nucleotides. After thorough washing, the gel-bound radioactivity was counted. The assay is faster than the conventional electrophoretic mobility shift assay because the gel electrophoresis step and the autoradiography step are eliminated. Moreover, the assay is very sensitive, allowing the detection of picomolar quantities of nucleotides, and is not affected by up to 50% dimethylsulfoxide, a solvent for hydrophobic inhibitors. Curcumin and dihydroguaiaretic acid, recently known inhibitors of Jun-Fos-DNA complex formation, were applied to this Jun-GST-fused Fos system and revealed to decrease the dimer-DNA binding.

Quantitative Assay for the Binding of Jun-Fos Dimer and Activator Protein-1 Site

Lee, Sang-Kyou,Park, Se-Yeon,Jun, Gyo,Hahm, Eun-Ryeong,Lee, Dug-Keun,Yang, Chul-Hak Korean Society for Biochemistry and Molecular Biol 1999 Journal of biochemistry and molecular biology Vol.32 No.6

The Jun and Fos families of eukaryotic transcription factors form heterodimers capable of binding to their cognate DNA enhancer elements. We are interested in searching for inhibitors or antagonists of the binding of the Jun-Fos heterodimer to the activator protein-1 (AP-1) site. The basic-region leucine zipper (bZIP) domain of c-Fos was expressed as a fusion protein with glutathione S-transferase, and allowed to form a heterodimer with the bZIP domain of c-Jun. The heterodimer was bound to glutathione-agarose, to which were added radiolabeled AP-1 nucleotides. After thorough washing, the gel-bound radioactivity was counted. The assay is faster than the coventional electrophoretic mobility shift assay because the gel electrophoresis step and the autoradiography step are eliminated. Moreover, the assay is very sensitive, allowing the detection of picomolar quantities of nucleotides, and is not affected by up to 50% dimethylsulfoxide, a solvent for hydrophobic inhibitors. Curcumin and dihydroguaiaretic acid, recently known inhibitors of Jun-Fos-DNA complex formation, were applied to this Jun-GST-fused Fos system and revealed to decrease the dimer-DNA binding.

Correlations Between Fasciology and Yin Yang Doctrine

Hui Tao,Mei-chun Yu,Hui-ying Yang,Rong-mei Qu,Chun Yang,Xin Zhou,Yu Bai,Jing-peng Wu,Jun Wang,Ou Sha,Lin Yuan 사단법인약침학회 2011 Journal of Acupuncture & Meridian Studies Vol.4 No.2

The aim of this study is to explore the correlations between fasciology and yin yang doctrine. Professor Yuan developed fasciology by three-dimensional reconstruction of connective tissue (fascia) in the trunk and limbs of the human body and tracing back to tissue origins in light of biological evolution and developmental biology. Fasciology states that the human body can be divided into two systems: the supporting-storing system and the functional system. This article elaborates on the roles of the two systems and their mutual relationship. The two systems are used to analyze the yin,the yang, and their relationship. The two systems are promoted but also restricted in different contexts. The supporting-storing system is formed by undifferentiated connective tissue and provides undifferentiated cells and nutrients for differentiated cells of the functional system. Thus, the supporting-storing system could be classified as quiet, similar to yin. The functional system continuously maintains the various functional activities of the human body. Thus, the functional system could be classified as active, similar to yang. In interpreting the yin yang doctrine from the point of view of fasciology, yin can be compared with the supporting-storing system and yang can be compared with the functional system.

Long-term Stability after Reduction of Mandible Fracture by Keyhole Plate: Evaluation at the Time of Plate Removal

Cheon, Kyeong-Jun,Cho, Seoung-Won,Jang, Won-Seok,Kim, Ju-Won,Yang, Byoung-Eun Korean Association of Maxillofacial Plastic and Re 2020 Maxillofacial Plastic Reconstructive Surgery Vol.42 No.-

Background: Various types of miniplates have been developed and used for the reduction of facial bone fractures. We introduced Yang's Keyhole (YK) plate, and reported on its short-term stability. The purpose of this study was to evaluate the long-term stability of the YK plate, as a follow-up study, by examining the patients who had used the YK plate among the patients with the reduction of mandible fractures and who visited for plate removal. Methods: We reviewed the medical records of 16 patients who underwent mandibular fracture fixation using a YK plate (group I) and 17 patients who underwent mandibular fracture fixation using a conventional plate (group II). Assessment was then made on malunion, occlusal stability, discomfort during the application, and clinical symptoms. Results: From January 2015 to December 2017, a total of 36 patients underwent mandibular fracture surgery using a YK plate. A total of 16 patients received plate removal. Among them, 15 were male and 1 female. The average age was 26 years. The applied surgical sites were the 12 on mandibular angle, 4 on mandibular symphysis, and 2 on subcondyle. The application period of YK plate was an average of 335 days. During the same period, 45 people underwent surgery on the conventional plate. A total of 17 patients received plate removal. Among them, 15 were male and 2 females. The average age was 36 years. The applied surgical sites were the 8 on mandibular angle, 4 on mandibular symphysis, and 2 on subcondyle. The application period of the conventional plate was an average of 349 days. No malocclusion occurred at the time of removal, and occlusion was stable. No patient complained of joint disease or discomfort. Conclusion: The YK plate system, in which the screw was first inserted and the plate was applied, for clinical convenience did not cause any particular problem and no significant difference from the conventional plate.

제어기를 이용한 사각 박육부재의 에너지흡수 제어특성

양용준,김정호,박준우,양인영 朝鮮大學校 機械技術硏究所 2007 機械技術硏究 Vol.10 No.1

승객의 안전을 도모하기 위해서는 충돌시 발생하는 구조부재의 에너지홉수특성을 적절히 제어하여 자동차의 구조를 설계해야한다. 현재 차량구조부재의 에너지흡수 능력을 향상시키 기 위해서 여러 가지 형상과 재질에 따른 연구가 활발히 진행되고 있다. 본 연구에서는 자동차 충돌시 에너지 흡수를 적절히 제어하여 승객의 안전을 도모할 수 있는 최적의 구조부재를 개발하고자 한다. 특히 자동차의 전면충돌시 에너지 흡수량이 가장 큰 부재인 사이드 부재의 기본형상인 사각단면부재에 대하여 제어기의 유무 제어기의 두께와 높이에 따른 에너지흡수능력과 평균압궤응력 및 변형모드를 고찰하였다. 에너지 흡수 제어기를 이용하여 에너지흡수량 및 평균압궤하중이 제어기를 사용하지 않은 경우보다 15∼20%정도 증가하였으며, 제어기의 높이와 두께 또한 에너지 흡수에 영향을 미쳤다. The structural members must be designed to control characteristics of energy absorption for protecting passengers in a car accident. Study on collapse characteristics of structural member is currently conducted in parallel with other studies on effective energy absorption capacity of structural members with diverse cross-sectional shapes and various materials. This study concerns the crashworthiness of the widely used vehicle structural members, square thin-walled tubes, which are excellent on the point of the energy absorption capacity. The absorbed energy, mean collapse load and deformation mode were analyzed for side member which absorbs most of the collision energy. To predict and control the energy absorption, controller is designed in consideration to its influence such as height. thickness and width ration in this study. The absorbed energy and mean collapse load of square tubes was increased by 15∼20% in using the controller and energy absorbing capability of the specimen was slightly changed by change of the high controller's height.

Effect of Entrepreneurship Education on Self-Efficacy and Entrepreneurial Motivation

Jun-Hwan Yang(양준환) 한국경영교육학회 2015 경영교육연구 Vol.30 No.4

최근 기업가정신은 지역경제와 국가경제를 활성화시키기 위한 핵심적인 기초 요인으로 주목 받아 왔다. 이러한 결과 대학에서의 기업가정신교육 중요성이 세계적으로 큰 관심을 불러일으켜 왔으며 또한 미래 기업가 양성을 위한 중요한 도구로 받아들여지고 있다. 중국은 최근 세계 경제대국으로 부상하고 있으며 한·중 양국 간의 경제의존도도 심화되어 가는 상황이다. 본 연구는 국가 간 비교연구로써 한국과 중국대학생들을 대상으로 기업가정신교육이 대학생들의 자기효능감과 기업가적 동기를 촉진시켜주는가를 규명하기 위하여 진행되었다. 회귀분석결과 기업가정신교육은 한국과 중국 대학생의 기업가적 동기에게 긍정적인 영향을 주는 것으로 나타났다. 기업가정신교육은 또한 대학생들의 자기효능감에도 유의한 영향을 주고 있음을 알 수 있었 다. 자기효능감은 대학생들의 기업가적 동기와 정(+)의 관계를 가지고 있으며 기업가정신교육과 기업가적 동기 사이에 유의한 부분 매개효과의 영향력을 보이는 것으로 나타났다. 본 연구는 기업가정신교육이 대학생들의 기업가적 동기를 촉진시키는 한편 자기효능감을 증진시킨다는 측 면에서 기업가정신 활성화를 위한 매우 중요한 선행변수임을 규명하였다. 이상의 연구결과에 대한 논의와 연구의 한계점을 제시하였다. The importance of entrepreneurship education is getting emphasized world-wide, since there has been general agreement on entrepreneurship as a vital element for revitalizing local and national economy. This study is designed to identify the practical role of entrepreneurship education whether it fosters self-efficacy and entrepreneurial motivation of university students as a cross-cultural study between Korea and China. Summary of the findings is as followings. Entrepreneurship education has a positive effect on entrepreneurial motivation for both Korean and Chinese university students. Self-efficacy of university students in both countries is influenced positively by entrepreneurship education. Finally, self-efficacy affects entrepreneurial motivation of students positively. Self-efficacy mediates partly the relationship between entrepreneurship education and entrepreneurial motivation in both countries. The findings imply that entrepreneurship education is the prerequisite factor in facilitating entrepreneurial activities among university students.

자판기 시스템 운영문제의 휴리스틱 해법 개발과 평가

박양병(Yang Byung Park),장원준(Won Jun Jang),박해수(Hae Soo Park) 한국산업경영시스템학회 2011 한국산업경영시스템학회지 Vol.34 No.4

The operation of vending machine system presents a decision-making problem which consists of determining the product allocation to vending-machine storage compartments, replenishment intervals of vending machines, and vehicle routes, all of which have critical effects on system profit. Especially, it becomes more difficult to determine the operation variables optimally when demand for a product that is out-of-stock spills over to another product or is lost. In this paper, we propose a heuristic for solving the operation problem of the vending machine system and evaluate it by comparing with Yang’s algorithm on various test problems with respect to system profit via a computer simulation. The results of computational experiments show a substantial profit increase of the proposed heuristic over Yang’s algorithm. Sensitivity analysis indicates that some input variables impact the profit increase significantly.

골수염의 진단에 있어서의 Tc-99m Hexamethylpropylene amine oxime 백혈구 스캔의 임상적 유용성

최상호,김양수,정준원,정진원,추은주,서동대,배인규,양승오,우준희,류지소 대한화학요법학회 2001 대한화학요법학회지 Vol.19 No.1

배경 : 골수염의 진단에 있어 Technetium-99m을 이용한 삼상 골스캔(Tc-99m MDP스캔)은 단순방사선 검사상 정상소견을 보이는 경우에 쓰이는 1차적 검사로 매우 높은 민감도를 보인다. 그러나 Tc-99m MDP 스캔은 골절이나, 정형외과적 삽입물, 신경병적 관절병변과 같이 뼈의 재형성이 증가되는 상황에서도 양성소견을 보여 특이도가 낮은 단점이 있다. 이러한 단점을 보완하고자 골수염의 진단에 있어 Tc-99m HMPAO 백혈구 스캔의 유용성을 알아보고자 하였다. 대상 및 방법 : 골수염이 의심되어 Tc-99m HMPAO 백혈구 스캔을 시행했던 15명의 의무기록을 후향적으로 분석하였다. 골수염의 확진은 임상관찰 소견과 배양검사 결과와 병리소견을 기준으로 하였다. 결과 : 골수염의 진단에 있어 Tc-99m HMPAO 백혈구 스캔의 민감도와 특이도는 각각 100%와 78%였고 Tc-99m HMPAO 스캔의 경우는 100%와 18%였다. 가장 흔한 원인균은 methicillin-resistant S. aureus였다. Tc-99m MDP 스캔에서 양성소견을 보였던 환자중 3명의 환자가 Tc-99m HMPAO 백혈구 스캔에서 음성 소견을 보였으며 이 환자들은 추적관찰상 골수염이 없는 것으로 확인되었다. 결론 : Tc-99m HMPAO 백혈구 스캔은 골수염의 존재를 진단하는데 있어 민감도와 특이도가 높으며, 특히 뼈의 재형성이 증가되어 Tc-99m MDP 스캔의 특이도가 낮게 나타나는 상황에서 유용하리라 사료된다. Background Three phase bone scintigraphy, performed with technetium-labeled diphosphonates (Tc-99m MDP scan), is the very sensitive radionuclide procedure of choice for diagnosing osteomyelitis. But, Tc-99m MDP scan is less specific when bone remodeling is increased. To overcome this limitation, we evaluated the diagnostic value of technetium-99m hexamethylpropyleneamine oxime (HMPAO) labeled leukocyte scan in osteomyelitis. Methods : We retrospectively reviewed 15 patients with suspected osteomyelitis who performed Tc-99m HMPAO labeled leukocyte scan. The diagnosis of osteomyelitis was based on clinical course and bacterial culture, and pathologic findings. Results The sensitivity and specificity of Tc-99m HMPAO leukocyte scan was 100% and 78%, and those of Tc-99m MDP scan was 100% and 18%, respectively. The most common organism was Methicilein-Resistant S. aureus. Among the patients that showed persistent accumulation on Tc-99m MDP scan, three patients showed no uptake on Tc-99m HMPAO leukocyte scan and these patients were confirmed that had no osteomyelitis. Conclusion : Tc-99m HMPAO leukocyte scan is a useful test for determining the presence or absence of osteomyelitis, especially under condition that bone remodelin is increased.

탄소섬유 적층혼성부재의 계면수 변화에 따른 축 압궤 특성

서현경,박준우,양용준,황우채,양인영 朝鮮大學校 機械技術硏究所 2007 機械技術硏究 Vol.10 No.1

알루미늄과 CFRP는 대표적인 경량화 재료이다. 알루미늄 부재는 안정적인 소성 변형에 의해 에너지를 흡수하며, CFRP 부재는 비강성과 비강도가 우수하고 불안정한 취성파괴로 인해 에너지를 흡수한다. 이러한 각각 알루미늄과 CFRP 부재의 압궤 특성을 기초로 두 재 료의 결합에 의해 서로의 장점에 대한 시너지 효과를 얻기 위해 혼성구조부재를 제작하였다. 본 연구에서는 알루미늄 부재의 외측을 CFRP로 강화시킨 혼성구조부채를 제작하여 CFRP의 계면수 변화에 따른 축 방향 준정적 압궤실험을 행하여 사각 및 원형 단변 모형의 혼성구조부재의 압궤 특성을 조사하였다. 실험 결과 혼성구조부재는 내부의 알루미늄 부재의 연성 성질 때문에 CFRP 부재의 불안정한 취성 파괴를 보완하여 비교적 안정적인 압궤 모드를 보였다. 하지만 혼성구조부재는 계면수 변화에 대해 에너지 흡수성과 압궤 모드는 별다른 영향을 보이지 않았다. The aluminum or CFRP is representative of light-weight materials. The aluminum tubes absorb energy by stable plastic deformation and the CFRP tubes absorbs energy by unstable brittle failure with higher specific strength and stiffness. Based on the respective collapse characteristics of aluminum and CFRP tubes, the aluminum CFRP compound tubes were manufactured to get a synergy effect when the aluminum CFRP tubes were combined with the advantages of each tube. This paper is to investigate the collapse characteristics of square or circular shaped aluminum CFRP compound tubes subjected to quasi-static axial collapse tests which were conducted by changing the stacking conditions. Test results showed that the collapse of the aluminum CFRP compound tubes complemented unstable brittle failure of the CFRP tube due to the ductile characteristics of the inner aluminum tube. It turned out that interlaminar numbers of aluminum CFRP compound tubes have no influence on the energy absorption and collapse modes of the tubes.