Roles of Immunohistochemical Staining in Diagnosing Pulmonary Squamous Cell Carcinoma

Yan, Yue,Zhang, Ya-Xiong,Fang, Wen-Feng,Kang, Shi-Yang,Zhan, Jian-Hua,Chen, Nan,Hong, Shao-Dong,Liang, Wen-Hua,Tang, Yan-Na,He, Da-Cheng,Wu, Xuan,Zhang, Li Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.2

Background: Differentiating morphologic features based on hematoxylin-eosin (HE) staining is the most common method to classify pathological subtypes of non-small-cell lung cancer (NSCLC). However, its accuracy and inter-observer reproducibility in pathological diagnosis of poorly differentiated NSCLC remained to be improved. Materials and Methods: We attempted to explore the role of immunohistochemistry (IHC) staining in diagnosing pulmonary squamous cell carcinoma (SQCC) with poorly differentiated features by HE staining or with elevated serum adenocarcinoma-specific tumor markers (AD-TMs). We also compared the difference of epidermal growth factor receptor (EGFR) mutation rate between patients with confirmed SQCC and those with revised pathological subtype. Logistic regression analyses were used to test the association between different factors and diagnostic accuracy. Results: A total of 132 patients who met the eligible criteria and had adequate specimens for IHC confirmation were included. Pathological revised cases in poor differentiated subgroup, biopsy samples and high-level AD-TMs cases were more than those with high/moderate differentiation, surgical specimens and normal-level AD-TMs. Moreover, biopsy sample was a significant factor decreasing diagnostic accuracy of pathological subtype (OR, 4.037; 95% CI 1.446-11.267, p=0.008). Additionally, EGFR mutation rate was higher in patients with pathological diagnostic changes than those with confirmed SQCC (16.7% vs 4.4%, p=0.157). Conclusions: Diagnosis based on HE staining only might cause pathological misinterpretation in NSCLC patients with poor differentiation or high-level AD-TMs, especially those with biopsy samples. HE staining and IHC should be combined as pathological diagnostic standard. The occurrence of EGFR mutations in pulmonary SQCC might be overestimated.

Examination of a Nurse-led Community-based Education and Coaching Intervention for Coronary Heart Disease High-risk Individuals in China

Yan-Jin Huang,Monica Parry,Ying Zeng,Yan Luo,Jing Yang,Guo-Ping He 한국간호과학회 2017 Asian Nursing Research Vol.11 No.3

Purpose: Early detection and management of coronary heart disease (CHD) are embedded into many community health service and primary care practices in western countries. The Framingham CHD risk score has been used to predict CHD and mortality for nearly 20 years, and it has predicted CHD event risk accurately in multiethnic populations. The aim of this study was to access the effect of a 6-month community-based intervention on CHD risk in individuals at high risk. Methods: A randomized controlled trial of individuals with a high 10-year CHD risk were recruited from two communities in China. Individuals in the intervention group (n ¼ 53) received a 3-month group education and a 3-month coaching session. Physical examination and self-report questionnaires were used to collect both pre- and postintervention data on blood pressure, glucose, cholesterol, body mass index, smoking, depression, and health-related quality of life (HRQoL). Results: A total of 102 participants (85.0%) completed the 6-month study. Compared with the usual care group, the intervention group had a 5 mmHg greater reduction in systolic blood pressure (t = 2.01, p = .047), larger declines in glucose (t = 2.49, p = .015), cholesterol (t = 2.44, p = .017), body mass index (t = 2.58, p = .011), and depression (t = 2.05, p = .043), and better reports of HRQoL (t = 3.36, p = .001). No significant group differences in smoking behaviors were reported. Conclusion: A 6-month community-based intervention in a CHD high-risk population improved diseaserelated risk factors, depression, and HRQoL. Results provide preliminary evidence for primary prevention of cardiovascular disease risk in a community high-risk population.

Post-Olympics Effect and The Sustainable Utilization of Peking Olympic Park

HE Yan,CHEN Tian 대한지리학회 2008 대한지리학회 컨퍼런스 자료집 Vol.2 No.-

High-resolution Anorectal Manometry in the Diagnosis of Functional Defecation Disorder in Patients With Functional Constipation: A Retrospective Cohort Study

Yan Zhao,Xiaoyang Ren,Wen Qiao,Lei Dong,Shuixiang He,Yan Yin 대한소화기 기능성질환∙운동학회 2019 Journal of Neurogastroenterology and Motility (JNM Vol.25 No.2

Background/Aims High-resolution anorectal manometry (HRAM) has been considered a first-line diagnostic tool for functional defecation disorder. However, clinical studies on HRAM used in constipation patients are very limited and few studies have reported the characteristics of anorectal pressure in Chinese patients. The aim of this study is to investigate the characteristics of motility data in a cohort of Chinese patients with functional constipation. Methods A total of 82 consecutive patients with functional constipation who underwent a standardized HRAM were retrospectively enrolled in this study. The functional defecation disorder was classified into Rao’s types. Results The mean age of 82 patients was 51 years (range, 16–83 years). Indications for anorectal manometry were functional constipation for all patients. The mean resting pressure was 69.2 ± 21.2 mmHg (range, 24.5–126.9 mmHg). The mean maximum squeezing pressure was 198.4 ± 75.6 mmHg (range, 54.2–476.9 mmHg). The mean length of the anal high pressure zone was 3.4 ± 1.0 cm (range, 0.6–4.9 cm). Sixty (73.2%) patients were diagnosed as functional defecation disorder. In attempted defecation, type I was most common (n = 24), followed by type II (n = 12), type III (n = 11), and type IV (n = 13) that were present on HRAM according to Rao’s classification. In all 60 patients with functional defecation disorder, 37 were women and 23 were men. Men were significantly more likely than women to have functional defecation disorder (92.0% vs 64.9%, P = 0.014). Conclusion HRAM could be used as a test for the diagnosis of functional defecation disorder and functional defecation disorder is common in Chinese patients with functional constipation.

Lysinibacillus tabacifolii sp. nov., a Novel Endophytic Bacterium Isolated from Nicotiana tabacum Leaves

Yan-Qing Duan,Song-Tao He,Qing-Qing Li,Ming-Feng Wang,Wen-Yuan Wang,Wei Zhe,Yong-Hong Cao,Ming-He Mo,Yu-Long Zhai,Wen-Jun Li 한국미생물학회 2013 The journal of microbiology Vol.51 No.3

A Gram-positive, catalase- and oxidase-positive, strictly aerobic, endospore-forming rod bacterium, designated K3514T, was isolated from the leaves of Nicotiana tabacum. The strain was able to grow at temperatures of 8–40°C, pH 5.0–10.0 and NaCl concentrations of 0–7%. The predominant quinones (>30%) of this strain were MK-7(H2) and MK-7. Phylogenetic analysis of 16S rRNA gene sequence showed that strain K3514T was affiliated to the genus Lysinibacillus, with its closest relatives being Lysinibacillus mangiferihumi (98.3% sequence similarity), Lysinibacillus sphaericus (97.9% sequence similarity), Lysinibacillus fusiformis (97.4% sequence similarity), and Lysinibacillus xylanilyticus (97.3% sequence similarity). However, low levels of DNA-DNA relatedness values suggested that the isolate was distinct from the other closest Lysinibacillus species. Additionally, based on analysis of morphological, physiological, and biochemical characteristics, the isolate could be differentiated from the closest known relatives. Therefore, based on polyphasic taxonomic data, the novel isolate likely represents a novel species, for which the name Lysinibacillus tabacifolii sp. nov. and the type strain K3514T (=KCTC 33042T =CCTCC AB 2012050T) are proposed.

Salivary Pepsin as an Intrinsic Marker for Diagnosis of Sub-types of Gastroesophageal Reflux Disease and Gastroesophageal Reflux Disease-related Disorders

( Yan-jun Wang ),( Xiu-qiong Lang ),( Dan Wu ),( Yu-qin He ),( Chun-hui Lan ),( Xiao-xiao ),( Bin Wang ),( Duo-wu Zou ),( Ji-min Wu ),( Yong-bin Zhao ),( Peter W Dettmar ),( Dong-feng Chen ),( Min Yan 대한소화기 기능성질환·운동학회 2020 Journal of Neurogastroenterology and Motility (JNM Vol.26 No.1

Background/Aims To determine the value of salivary pepsin in discriminating sub-types of gastroesophageal reflux disease (GERD) and GERD-related disorders. Methods Overall, 322 patients with different sub-types of GERD and 45 healthy controls (HC) were studied. All patients took Gastroesophageal Reflux Disease Questionnaire (GerdQ) and underwent endoscopy and 24-hour esophageal pH monitoring and manometry. Salivary pepsin concentration (SPC) was detected by using colloidal gold double-antibody immunological sandwich assay. Oral esomeprazole treatment was administrated in the patients with non-erosive reflux disease (NERD) and extra-esophageal symptoms (EES). Results Compared to HC, patients with erosive esophagitis, NERD, EES, EES plus typical GERD symptoms, or Barrett’s esophagus had a higher prevalence of saliva and SPC (all P < 0.001). There was no significant difference in the positive rate for pepsin in patients with functional heartburn or GERD with anxiety and depression, compared to HC. After esomeprazole treatment, the positive rate and SPC were significantly reduced in NERD (both P < 0.001) and in EES (P = 0.001 and P = 0.002, respectively). Of the 64 NERD patients, 71.9% (n = 46) were positive for salivary pepsin, which was significantly higher than the rate (43.8%, n = 28) of pathological acid reflux as detected by 24-hour esophageal pH monitoring (P = 0.002). Conclusions Salivary pepsin has an important significance for the diagnosis of GERD and GERD-related disorders. Salivary pepsin and 24-hour esophageal pH monitoring may complement with each other to improve the diagnostic efficiency.

Genetic Variation in PDCD6 and Susceptibility to Lung Cancer

He, Yan-Qi,Zhou, Bin,Shi, Shao-Qing,Zhang, Lin,Li, Wei-Min Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.9

Lung cancer is the most common type of cancer and one of the leading causes of death in the world. Genetic factors play an important role in its development. PDCD6, the encoding gene for programmed cell death protein 6, may function as a tumor suppressor gene. Non-small cell lung cancer (NSCLC) contributes about 80% to newly histologically diagnosed lung cancer patients. To explore the relationship between PDCD6 and NSCLC, we examined two single nucleotide polymorphisms(rs3756712 G/T andrs4957014 G/T, both in the intron region) of the PDCD6gene.A hospital-based case-control study was carried out including 302 unrelated NSCLC patients and 306 healthy unrelated subjects. Significantly increased NSCLC risk was found to be associated with the T allele of rs4957014 (P=0.027, OR=0.760, 95%CI=0.596-0.970). The genotype and allele frequencies of rs3756712 did not shown any significant difference between NSCLC group and controls (P=0.327, OR=0.879, 95%CI=0.679-1.137). In conclusion, we firstly demonstrated the association between the PDCD6 gene and risk of NSCLC in a Chinese Han population.

The dynamic stability analysis of guyed masts under random wind loads

He, Yan-Li,Chen, Wu-Jun,Dong, Shi-Lin,Wang, Zhao-Min Techno-Press 2003 Wind and Structures, An International Journal (WAS Vol.6 No.2

On the basis of the first Lyapunov stability theory, this paper develops a dynamic stability criterion for elastic structural systems under arbitrary dynamic loads, and shows the stability criterion using energy variation. After the dynamic stability criterion is validated through a classic example, it is used for the dynamic stability investigation of practical guyed masts under random wind loads. The criterion is reliable, simple and of advantage for structures with large number of elements and nodes. The slack guys and internal resonance between guys and mast are two main factors which induces the dynamic instability of guyed masts, at the same time, some dynamic stability characteristics of guyed masts are found.

Investigations into film cooling and unsteady flow characteristics in a blade trailing-edge cutback region

Yan Gao,Xin Yan,Jun Li,Kun He 대한기계학회 2018 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.32 No.10

Unsteady flow characteristic and film cooling effect in the blade trailing edge cutback region are numerically investigated using DES (detached eddy simulation) method at three blowing ratios, four lip thickness ratios and three rib geometries. With the experimental data, reliability and accuracy of the numerical methods are validated. The results indicate that, compared with RANS (Reynolds-averaged Navier-Stokes) equations solutions, the DES method has a superior accuracy in predicting the film cooling effectiveness on trailing-edge cutback. The lip thickness has a pronounced effect on shedding vortex scale downstream the slot. As the lip thickness ratio decreases, the film cooling effect on trailing edge cutback is improved. Compared with the line ribs, the arrangement of pin fin ribs enhances the uniformity of coolant coverage on trailing edge cutback along lateral direction. However, with different types of rib, the film cooling effectiveness on the trailing edge cutback is mainly determined by the stable cooling flow structures and mixing effect between mainstream and coolant. The development of vortex system downstream the slot is significantly affected by the blowing ratio, which in turn influences the film cooling effect on trailing edge cutback.

Effect and Mechanism of Sulfates on Dispersity of Polycarboxylate-type Superplasticizers

Yan He,Xiong Zhang,Jian Guan 대한토목학회 2017 KSCE JOURNAL OF CIVIL ENGINEERING Vol.21 No.6

The effects of different sulfates on fluidity, yield stress, and apparent viscosity of cement paste with polycarboxylate-type superplasticizer were investigated. The results showed that sulfates with alkali ions (such as Na2SO4 and K2SO4) had a pronounced negative effect on the dispersity of PC superplasticizer, while the effect of indissolvable sulfate (such as CaSO4·2H2O) on the dispersity of PC superplasticizer was slight. The mechanism of sulfates on dispersibility of polycarboxylate superplasticizer was analyzed through zeta potential and equilibrium adsorption capacity. It was indicated that with the increase of dissolution rate and dissolution speed, and under the effects of alkali ions, the competitive adsorption between sulfate ions and polycarboxylate superplasticizer was enhanced, the absolute value of zeta potential decreased as double electrode layer was destroyed, the aggregation behavior of PC superplasticizer was boosted with larger aggregation size, and the calcium ion concentration in the interstitial solution was generated. In this way, the electrostatic repulsion of cement particles was weakened; the flocculation structure became more and stronger. Under certain shearing rate, less degree of flocculation structure was damaged, which meant the decrease of fluidity and rheological properties in cement paste.