Bioinformatic prediction and analysis of glucolipid metabolic regulation by miR-34a in Megalobrama amblycephala

Ling‑Hong Miao,Wen‑Jing Pan,Yan Lin,Bo Liu,Ming‑Chun Ren,Qun‑Lan Zhou,Xian‑Ping Ge 한국유전학회 2017 Genes & Genomics Vol.39 No.12

The objective of this study was to analyze the target genes and regulatory function of miR-34a in Megalobrama amblycephala using second-generation highthroughput sequencing and bioinformatic tools. Functional enrichment analysis was performed by gene ontology. MiR- 34a and target gene expression levels were measured in M. amblycephala fed normal and high-carbohydrate diets. The results revealed that miR-34a was highly conserved in several species, and miR-34a of M. amblycephala has a close evolutionary relationship to that of zebrafish and common carp. miRanda, TargetScan, RNAhybrid predicted 5,185, 6,282 and 2,168 target genes, respectively, and 645 target genes were in common. According to annotation information, the target genes were enriched in phosphate metabolism, glycerophospholipid metabolism, Golgi vesicle transport, cell division, and other biological processes (P < 0.05). Pathway enrichment analysis revealed that these target genes were mainly enriched in alpha-linolenic acid and linoleic acid metabolism, ether lipid metabolism, VEGF signaling pathway, Fc epsilon RI signaling pathway, GnRH signaling pathway, and MAPK signaling pathway (P < 0.05). The regulatory role of miR-34a was more significant in the liver than in the brain of M. amblycephala. MiR-34a regulates glucose lipid homeostasis induced by high glucose diets by upregulating hepatic PI3K/Akt, FOXO, and TOR signaling pathways.

Synthesis, Phase Behavior, and Simulated In vitro Degradation of Novel HTPB-b-PEG Polyurethane Copolymers

Yan-Ling Luo,Yan Miao,Feng Xu 한국고분자학회 2011 Macromolecular Research Vol.19 No.12

Two types of polyurethanes with alternating and random block architectures, hydroxyl-terminated liquid polybutadiene and poly(ethylene glycol) block copolymers (HTPB-alt-PEG and HTPB-co-PEG), were synthesized using a coupling reaction route between the hydroxyl groups and the isocyanate groups. The chemical and crystal structures were characterized using Fourier transform-infrared spectroscopy (FTIR) and X-ray diffraction, while phase behavior was examined using scanning electron microscopy (SEM) and differential scanning calorimetry. The biodegradation in a simulated human body fluid was investigated through mass loss, SEM, and FTIR. The experimental results indicated that all of the polyurethane samples bore the microphase separation structure, and the separation degree depended on the sequence structure and molecular weight (MW) of PEG and further affected their in vitro degradation. The driving force was related to the restricted movement of the molecular segments, the crystallization of the soft/hard phases, and/or the hydrogen bonding interactions between the hard segments. The surface morphological change of the degraded samples further demonstrated that the degradation became serious as the PEG MW increased and that the random block copolymers decomposed more easily than the alternating copolymers. The block polymer materials are expected to be incorporated into specific applications in related biomedical fields.

ASPEN PLUG SIMULATION OF MOVING-BED CHAR GASIFICATION USING FOUR TYPES OF MODELS

Ling Yan DENG,Hyung Taek KIM 한국신재생에너지학회 2015 AFORE Vol.2015 No.11

Disease vector occurrence and ecological characteristics of chiggers on the chestnut white-bellied rat Niviventer fulvescens in Southwest China between 2001 and 2019

Yan-Ling Chen,Xian-Guo Guo,Wen-Yu Song,Tian-Guang Ren,Lei Zhang,Rong Fan,Cheng-Fu Zhao,Zhi-Wei Zhang,Wenge Dong,Xiao-Bin Huang,Dao-chao Jin 대한기생충학ㆍ열대의학회 2023 The Korean Journal of Parasitology Vol.61 No.3

Chigger mites are the vector of scrub typhus. This study estimates the infestation status and ecological characteristics of chiggers on the chestnut white-bellied rat Niviventer fulvescens in Southwest China between 2001 and 2019. Chiggers were identified under the microscope, and infestation indices were calculated. The Preston’s log-normal model was used to fit the curve of species abundance distribution. A total of 6,557 chiggers were collected in 136 of 342 N. fulvescens rats, showing high overall infestation indices (prevalence=39.8%, mean abundance=19.2, mean intensity=48.2) and high species diversity (S =100, H’=3.0). Leptotrombidium cangjiangense, Neotrombicula japonica, and Ascoschoengastia sifanga were the three dominant chigger species (constituent ratio=42.9%; 2,736/6,384) and exhibited an aggregated distribution among different rat individuals. We identified 100 chigger species, with 3 of them (Leptotrombidium scutellare, Leptotrombidium wenense, and Leptotrombidium deliense) as the main vectors of scrub typhus in China and nine species as potential vectors of this disease. Disease vector occurrence on N. fulvescens may increase the risk of spreading scrub typhus from rats to humans. Chigger infestation on N. fulvescens varied significantly in different environments. The species abundance distribution showed a log-normal distribution pattern. The estimated number of chigger species on N. fulvescens was 126 species.

Node-Level Trust Evaluation Model Based on Blockchain in Ad Hoc Network

Yan, Shuai-ling,Chung, Yeongjee The Institute of Internet 2019 International journal of advanced smart convergenc Vol.8 No.4

Due to the characteristics of an ad hoc network without a control center, self-organization, and flexible topology, the trust evaluation of the nodes in the network is extremely difficult. Based on the analysis of ad hoc networks and the blockchain technology, a blockchain-based node-level trust evaluation model is proposed. The concepts of the node trust degree of the HASH list on the blockchain and the perfect reward and punishment mechanism are adopted to construct the node trust evaluation model of the ad hoc network. According to the needs of different applications the network security level can be dynamically adjusted through changes in the trust threshold. The simulation experiments demonstrate that ad-hoc on-demand distance vector(AODV) Routing protocol based on this model of multicast-AODV(MAODV) routing protocol shows a significant improvement in security compared with the traditional AODV and on-demand multipath distance vector(AOMDV) routing protocols.

Protective effects of l-theanine on rats with dextran sulfate sodium-induced infl ammatory bowel disease

Ling Chen,Wen-jun Xiao,Qiong-xian Yan,Zhi-hua Gong,Sheng Zhang,Li Zeng,Ming Yang,Yan-he Zhou 대한약학회 2020 Archives of Pharmacal Research Vol.43 No.8

The aim of this study is to evaluate the antiinflammatory and protective eff ects of L -theanine in infl ammatorybowel disease (IBD) and to identify the underlyingmolecular mechanisms. Rats were pre-treated with L -theanineat 0, 50, 200, or 800 mg/kg/day. IBD was induced inrats using dextran sulfate sodium (DSS). Histopathologicalanalysis suggests that L -theanine can suppress DSS-inducedIBD with signifi cant inhibition of infl ammation in large andsmall intestinal tissues. Moreover, the 200 mg/kg/day L -theanine-treated DSS group had higher body and small intestineweights, a lower disease activity index and expression ofinfl ammatory factors than the DSS group without pre-treatment. In RNA sequencing and tandem mass tag labelinganalyses, large number of mRNAs and proteins expressionlevel diff ered when compared with the DSS-induced ratswith and without 200 mg/kg/day L -theanine pre-treatment. Moreover, Kyoto Encyclopedia of Genes and Genomes pathwayanalysis indicates the anti-infl ammatory activities ofL -theanine in DSS-induced IBD, with a high representationof genes in “Cholesterol metabolism” and “Retinol metabolism”pathways. Analysis of protein–protein interaction networksfurther indicates the involvement of these two pathways. These studies suggest that medium-dose L -theaninepre-treatment could ameliorate DSS-induced IBD throughmolecular mechanisms involving cholesterol and retinolmetabolism.

PVDF/PS/HDPE/MWCNTs/Fe3O4 nanocomposites: Effective and lightweight electromagnetic interference shielding material through the synergetic effect of MWCNTs and Fe3O4 nanoparticles

Ling-yan Li,Shuang-lin Li,Yan Shao,Rui Dou,Bo Yin,Ming-bo Yang 한국물리학회 2018 Current Applied Physics Vol.18 No.4

In this work, Polyvinylidene Fluoride (PVDF)/polystyrene (PS)/high density polyethylene (HDPE) ternary blends displayed a core-shell structure where HDPE was the core, PS was the shell, and this core-shell system dispersed in PVDF matrix. Here, multiwall carbon nanotubes (MWCNTs) and ferroferric oxide (Fe3O4) was incorporated. F-F composites with MWCNTs was in PS shell and Fe3O4 was in PVDF matrix and E-F composites with MWCNTs was in PS shell and Fe3O4 was in HDPE core were fabricated by melt blending. It was indicated that the core-shell morphology between PS and HDPE was well retained with the incorporation of Fe3O4 and MWCNTs. Both the electrical conductivity of F-F and E-F composites were similar without no obvious change with the incorporation of Fe3O4. Composites with greater than 20 dB shielding effectiveness were easy to obtain. The highest SE we observed was for the F-F composite with 1 vol% Fe3O4 and 1 vol%MWCNTs was 25 dB at 9.5 GHz, and the SE was over 20 dB in the whole measured frequency(X-band). The E-F composites with SE greater than 20 dB in X-band was at 2 vol% Fe3O4 and 1 vol% MWNCTs. Such effective and lightweight nanocomposites were obtained, resulting from the synergetic effect of MWCNTs and Fe3O4 nanoparticles.

Development and Application of Tea Saponins: A Review

Ling-Yan Jia,You-Ying Tu 한국차학회 2015 한국차학회지 Vol.- No.S

Tea saponins are triterpenoid saponins from Camellia plants, and consist of a sapogenin, glycosides and organic acids. Up to 2013, at least 93 tea saponin monomers from the seeds, roots and flowers of tea plants have been isolated and identifined. Tea saponins are excellent natural biosurfactant, and possess a variety of pharmacological activities such as anti-inflammatory, anti-fungal, anti-bacterial, anti-cancer, hypolipidemic and gastric mucosal protection. In addition, tea saponins can be used for the recovery of heavy metals from wastewater and contaminated soils due to their high binding capacity with metal ions. These properties make tea saponins can be applied in pesticides, feed, aquaculture, textile, pharmaceuticals, cosmetics, building materials and environmental modification.Therefore, this article reviews the latest studies on tea saponins, and discuss the comprehensive utilization of tea saponins in the future

The Signing of the Geneva Convention of 1864 and the Creation of China Red Cross Society

Ling Yan 대한적십자사 인도법연구소 2014 人道法論叢 Vol.- No.34

Synthesis of Composite Particles with Fe3O4 core and Ag Shell for the Development of Fingerprints

Ling-Yan Zhang,Ting Chu 대한화학회 2013 Bulletin of the Korean Chemical Society Vol.34 No.5

The Fe3O4 -core and Ag- shell (Fe3O4@Ag nanoeggs) were prepared through the encapsulation of 3- aminopropyltriethoxysilane-coated magnetite nanoparticle in nano-Ag shell by a simple chemically controlled procedure. The Fe3O4@Ag nanoeggs were characterized by scanning electron microscopy, transmission electron microscopy, UV-vis spectrum and superconducting quantum interference device magnetometer, respectively. A detailed analysis is provided of how the hydrolysis and condensation of 3-aminopropyltriethoxysilane and the pH value are vital in fabricating the Fe3O4@Ag nanoeggs. The prepared Fe3O4@Ag nanoeggs possessed uniform size, improved monodispersity, stability against aggregation and high magnetization, which were utilized for the detection of latent fingerprints deposited onto different surfaces. The experimental results showed that the latent fingerprints developed with the Fe3O4@Ag nanoeggs powders exhibited excellent ridge details with minimal background staining.