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      • Analysis of High Frequency Voltage-Resonat Push-Pull Converter

        Kenichi Onda,Takao Yaginuma,Yasuo Matsuda,Akiteru Ueda,Hitachi Ltd 전력전자학회 1989 ICPE(ISPE)논문집 Vol.- No.-

        In a conventional analysis of a voltage-reso-nant push-pul] converter, resonant frequency and out-put voltage are obtained as constant, even if the out-put current of the converter changes. However, experi-mentally these values are known to vary with the out-put current. No qualitative or quantitative interpre-tation has been made on this point yet. This paper describes a new analysis of the converter, considering leakage inductance of a transformer. The variation is found to be caused by parasitic resonance which occurs between the leakage inductance of the transformer and the capacitance of the resonant capacitor. The new method allows the characteristics of the converter to be analyzed quantitatively. Then the varlation of the resonant frequency and the output voltage can be decreased through an increase of the coupling coefficient of the transformer or the resonant current of the converter. Furthermore, the generalized characteristics of the voltage-resonant push-pull converter can be derived from this analysis.

      • KCI등재

        A microfluidic device for partial cell separation and deformability assessment

        Diana Pinho,Tomoko Yaginuma,Rui Lima 한국바이오칩학회 2013 BioChip Journal Vol.7 No.4

        Blood flow in microcirculation shows several interesting phenomena that can be used to develop microfluidic devices for blood separation and analysis in continuous flow. In this study we present a novel continuous microfluidic device for partial extraction of red blood cells (RBCs) and subsequent measurement of RBC deformability. For this purpose, we use polydimethylsiloxane (PDMS) microchannels having different constrictions (25%, 50% and 75%) to investigate their effect on the cell-free layer (CFL)thickness and separation efficiency. By using a combination of image analysis techniques we are able to automatically measure the CFL width before and after an artificial constriction. The results suggest that the CFL width increases with enhancement of the constriction and contributes to partial cell separation. The subsequent measurements of RBCs deformation index reveal that the degree of deformation depends on the constriction geometries and hematocrit after the cell separation module. The proposed microfluidic device can be easily transformed into a simple, inexpensive and convenient clinical tool able to perform both RBC separation and deformability analysis in one single device. This would eliminate the need for external sample handling and thus reducing associated labor costs and potential human errors.

      • KCI등재

        Extensional flow-based microfluidic device: deformability assessment of red blood cells in contact with tumor cells

        Vera Faustino,Rui Lima,Diana Pinho,Tomoko Yaginuma,Ricardo C. Calhelha,Isabel C.F.R. Ferreira 한국바이오칩학회 2014 BioChip Journal Vol.8 No.1

        Red blood cell (RBC) deformability has become one of the important factors to assess blood and cardiovascular diseases. The interest on blood studies have promoted a development of various microfluidic devices that treat and analyse blood cells. Recet years, besides the RBC deformability assessment, these devices are often applied to cancer cell detection and isolation from the whole blood. The devices for cancer cell isolation rely mainly on size and deformability of the cells. However, the examination of deformability of the RBCs mixed with cancer cells is lacking. This study aims at determining the deformation index (DI) of the RBCs in contact with cancer cells using a hyperbolic microchannel which generates a strong extensional flow. The DIs of human healthy RBCs and human RBCs in contact with a tumor cell line (HCT-15, colon carcinoma) were compared by analyzing the flowing RBCs images captured by a high speed camera. The results reveal that the RBCs that were in contact with HCT-15 cells have lower deformability than the normal RBCs.

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