Comparison of the Genomic Structure of the Heat Shock Protein-88(Hsp88) Genes in the Four Entomopathogenic Fungal Strains, Paecilomyces tenuipes Jocheon-1, P. tenuipes, Cordyceps militaris, and C. pruinosa

( Ya Qi Liu ),( Nam Sook Park ),( Yong Gyun Kim ),( Keun Ki Kim ),( Hyun Chul Park ),( Hong Joo Son ),( Sang Mong Lee ) 한국잠사학회 2012 International Journal of Industrial Entomology Vol.25 No.1

Comparison on the genomic structure and phylogenetic relationship of the Hsp88 genes from P. tenuipes Jochoen-1, P. tenuipes, C. militaris and C. pruinosa was described. The Hsp88 genes from the three entomopathogenic strains, P. tenuipes Jocheon-1(strain), P. tenuipes(original species), and C. militaris contain the identical genomic structure, namely 5 introns and 6 exons with the length of 13, 62, 32, 1,438, 306, 288 nucleotides encoding 713 amino acid residues, whereas in case of C. pruinosa, it contains 4 introns and 5 exons with the length of 13, 62, 32, 1,744, 288 nucleotides encoding 713 amino acid residues. The genomic DNA length of the Hsp88 genes from P. tenuipes Jocheon-1 and P. tenuipes are both 2,600 nucleotides long in size. The Hsp88 genes from C. militaris and C. pruinosa are 2,582, 2,576 nucleotides long in size, respectively. Hsp88 genes of the P. tenuipes Jochoen-1, P. tenuipes, C. militaris and C. pruinosa also contain the conserved ATP-binding domain. Phylogenetic analysis of the Hsp genes of the four strains tested in this study showed that the fungal Hsp88 is divided into two separate clades, ascomycetes and deutromycete. Within the ascomycetes fungal clade, the P. tenuipes Jochoen-1 and P. tenuipes formed a subgroup, on the other hand, C. militaris and C. pruinosa formed another subgroup. Pair-wise comparison of P. tenuipes Jocheon-1 Hsp88 with those of P. tenuipes, C. militaris and C. pruinosa Hsp88s revealed significant identity in deduced amino acid sequence among these strains. The P. tenuipes Jocheon-1 Hsp88 showed 99% identity with the P. tenuipes, 97% identity with the C. militaris, and 98% identity with the C. pruinosa.

Molecular Cloning of the cDNA of Heat Shock Protein 88 Gene from the Entomopathogenic Fungus, Paecilomyces tenuipes Jocheon-1

( Ya Qi Liu ),( Nam Sook Park ),( Yong Gyun Kim ),( Keun Ki Kim ),( Hyun Chul Park ),( Hong Joo Son ),( Chang Ho Hong ),( Sang Mong Lee ) 한국잠사학회 2014 International Journal of Industrial Entomology Vol.28 No.2

The full-length heat shock protein 88 (HSP88) complementary DNA (cDNA) of Paecilomycestenuipes Jocheon -1 was obtained by screening the Paecilomyces tenuipes (P. tenuipes )Jocheon-1 Uni-Zap cDNA library and performing 5` RACE polymerase chain reaction(PCR). The P. tenuipes Jocheon-1 HSP88 cDNA contained an open reading frame (ORF) of2, 139-basepair encoding 713 amino acid residues. The deduced amino acid sequence of theP. tenuipe s Jocheon-1 HSP88 cDNA showed 77% identity to Nectria haematococca HSP88and 45-76% identity to other fungal homologous HSP88s. Phylogenetic analysis and BLAST program analysis confirmed that the deduced amino acid sequences of the P. tenuipesJocheon-1 HSP88 gene belonged to the ascomycetes group within the fungal clade. The P. tenuipes Jocheon-1 HSP88 also contained the conserved ATPase domain at the N-terminalregion. The cDNA encoding P. tenuipes Jocheon-1 HSP88 was expressed as an 88 kilodalton(kDa) polypeptide in baculovirus-infected insect Sf9 cells. Under higher temperature conditionsfor the growth of the entomopathogenic fungus, mRNA expression of P. tenuipes Jocheon-1HSP88 was quantified by real time PCR (qPCR). The results showed that heat shock stressinduced a higher level of mRNA expression compared to normal growth conditions.

Molecular Cloning, Expression and Characterizaion of Heat Shock Protein Gene from Paecilomyces tenuipes Jocheon-1

Liu Ya Qi,Nam Sook Park,Han Seok Kang,Seon Ku Kim,Yong Gyun Kim,Byung Uuk Cho,Teak Soon Shin,Keun Ki Kim,Hyun Chul Park,Hong Joo Son,Hong Gu Lee,Sang Mong Lee 한국응용곤충학회 2011 한국응용곤충학회 학술대회논문집 Vol.2011 No.05

In this study, a full-length heat shock protein88 complementary DNA (cDNA) of Paecilomyces tenuipes Jocheon-1 was obtained by screening of P. tenuipesJocheon-1 Uni-Zap cDNA library and 5' RACE polymerase chain reaction. The Paecilomyces tenuipes Jocheon-1 heat shock protein88 cDNA contains an open reading frame of 2,139 bp encoding 713 amino acid residues. The deduced amino acid sequence of the P. tenuipes Jocheon-1 HSP88 cDNA showed 77% identity to N. haematococca HSP88 and 45-76% identity to other fungi HSP88. Phylogenetic analysis and BLAST program analysis confirmed that the deduced amino acid sequences of the P. tenuipes Jocheon-1 HSP88 gene belonged to the ascomycetes group within the fungal clade and P. tenuipes Jocheon-1 HSP88 also contains the conserved ATPase domain at the N-terminal. The cDNA encoding P. tenuipes Jocheon-1 HSP88 was expressed as a 88 kDa polypeptide in baculovirus-infected insect Sf9 cells. Under different stress conditions, mRNA expression of P. tenuipes Jocheon-1 HSP88 were quantified by real-time PCR and the result showed that heat shock stress affected the mRNA expression levels of P. tenuipes Jocheon-1 HSP88.

Comparison of the Genomic Structure of the Heat Shock Protein-88(Hsp88) Genes in the Four Entomopathogenic Fungal Strains, Paecilomyces tenuipes Jocheon-1, P. tenuipes, Cordyceps militaris, and C. pruinosa

Liu, Ya-Qi,Park, Nam-Sook,Kim, Yong-Gyun,Kim, Keun-Ki,Park, Hyun-Chul,Son, Hong-Joo,Lee, Sang-Mong Korean Society of Sericultural Science 2012 International Journal of Industrial Entomology Vol.25 No.1

Comparison on the genomic structure and phylogenetic relationship of the Hsp88 genes from P. tenuipes Jochoen-1, P. tenuipes, C. militaris and C. pruinosa was described. The Hsp88 genes from the three entomopathogenic strains, P. tenuipes Jocheon-1(strain), P. tenuipes(original species), and C. militaris contain the identical genomic structure, namely 5 introns and 6 exons with the length of 13, 62, 32, 1,438, 306, 288 nucleotides encoding 713 amino acid residues, whereas in case of C. pruinosa, it contains 4 introns and 5 exons with the length of 13, 62, 32, 1,744, 288 nucleotides encoding 713 amino acid residues. The genomic DNA length of the Hsp88 genes from P. tenuipes Jocheon-1 and P. tenuipes are both 2,600 nucleotides long in size. The Hsp88 genes from C. militaris and C. pruinosa are 2,582, 2,576 nucleotides long in size, respectively. Hsp88 genes of the P. tenuipes Jochoen-1, P. tenuipes, C. militaris and C. pruinosa also contain the conserved ATP-binding domain. Phylogenetic analysis of the Hsp genes of the four strains tested in this study showed that the fungal Hsp88 is divided into two separate clades, ascomycetes and deutromycete. Within the ascomycetes fungal clade, the P. tenuipes Jochoen-1 and P. tenuipes formed a subgroup, on the other hand, C. militaris and C. pruinosa formed another subgroup. Pair-wise comparison of P. tenuipes Jocheon-1 Hsp88 with those of P. tenuipes, C. militaris and C. pruinosa Hsp88s revealed significant identity in deduced amino acid sequence among these strains. The P. tenuipes Jocheon-1 Hsp88 showed 99% identity with the P. tenuipes, 97% identity with the C. militaris, and 98% identity with the C. pruinosa.

Genomic Structure of the Heat Shock Protein88 genes of Paecilomyces tenuipes Jocheon-1, Paecilomyces tenuipes, Cordyceps militaris & Cordycepspruinosa

Liu Ya Qi,Nam Sook Park,Han Seok Kang,Seon Ku Kim,Yong Gyun Kim,Byung Uuk Cho,Teak Soon Shin,Keun Ki Kim,Hyun Chul Park,Hong Joo Son,Hong Gu Lee,Sang Mong Lee 한국응용곤충학회 2011 한국응용곤충학회 학술대회논문집 Vol.2011 No.05

The genomic structure and phylogenetic relationships of HSP88 genes from P. tenuipes Jocheon-1, P. tenuipes, C. militaris and C. pruinosa are described. The HSP88 genomic DNA from P. tenuipes Jocheon-1, P. tenuipes and C. militaris all contain 5 introns and 6 exons with the length of 13, 62, 32, 1438, 306, 288 bp, encoding 713 amino acid residues. C. pruinosa HSP88 genomic DNA contains 4 introns and 5 exons encoding 713 amino acids. The length of each exon of C. pruinosa HSP88 is 13, 62, 32, 1744, 288 bp and the length of exon 4 is identical to the total length of exon 4 and exon 5 of HSP88 of P. tenuipes Jochoen-1, P. tenuipes, and C. militaris. The deduced amino acid sequence of P. tenuipes Jocheon-1 HSP88 showed 99% identity with the P. tenuipes, 97% identity with the Cordyceps militaris, and 98% identity with the C. pruinosa. Phylogenetic analysis confirmed that the P. tenuipes Jocheon-1, P. tenuipes, C. militaris and C. pruinosa HSP88 are placed together within the ascomycetes group of fungal clade.

Molecular Cloning of the cDNA of Heat Shock Protein 88 Gene from the Entomopathogenic Fungus, Paecilomyces tenuipes Jocheon-1

Liu, Ya-Qi,Park, Nam Sook,Kim, Yong Gyun,Kim, Keun Ki,Park, Hyun Chul,Son, Hong Joo,Hong, Chang Ho,Lee, Sang Mong Korean Society of Sericultural Science 2014 International Journal of Industrial Entomology Vol.28 No.2

The full-length heat shock protein 88 (HSP88) complementary DNA (cDNA) of Paecilomyces tenuipes Jocheon-1 was obtained by screening the Paecilomyces tenuipes (P. tenuipes) Jocheon-1 Uni-Zap cDNA library and performing 5' RACE polymerase chain reaction (PCR). The P. tenuipes Jocheon-1 HSP88 cDNA contained an open reading frame (ORF) of 2,139-basepair encoding 713 amino acid residues. The deduced amino acid sequence of the P. tenuipe s Jocheon-1 HSP88 cDNA showed 77% identity to Nectria haematococca HSP88 and 45-76% identity to other fungal homologous HSP88s. Phylogenetic analysis and BLAST program analysis confirmed that the deduced amino acid sequences of the P. tenuipes Jocheon-1 HSP88 gene belonged to the ascomycetes group within the fungal clade. The P. tenuipes Jocheon-1 HSP88 also contained the conserved ATPase domain at the N-terminal region. The cDNA encoding P. tenuipes Jocheon-1 HSP88 was expressed as an 88 kilodalton (kDa) polypeptide in baculovirus-infected insect Sf9 cells. Under higher temperature conditions for the growth of the entomopathogenic fungus, mRNA expression of P. tenuipes Jocheon-1 HSP88 was quantified by real time PCR (qPCR). The results showed that heat shock stress induced a higher level of mRNA expression compared to normal growth conditions.

Function and Oligomerization Study of the Leucine Zipper-Like Domain in P13 from Leucania separata Multiple Nuclear Polyhedrosis Virus

( En Qi Du ),( Lun Guang Yao ),( Hua Xu ),( Song Ya Lu ),( Yi Peng Qi ) 생화학분자생물학회 2007 BMB Reports Vol.40 No.2

고맥락 문화 차원에서 한자 의미의 심미적 특징 연구

치야쉔(Qi, Ya Xuan),장주영(Chang, Ju Young) 한국디자인문화학회 2021 한국디자인문화학회지 Vol.27 No.2

중국은 전형적인 고맥락 문화 국가로서, 이러한 측면에서 중국인의 표현 방식에는 중국만의 고유한 특징이 있습니다. 한자는 중국 문명의 전승과 사상의 전파, 그리고 일상의 교류를 위한 중요한 매개체이자 언어 환경의 중요한 구성부분으로서, 독특한 구조와 표현체계가 존재합니다. 특히, 의미에 있어서 저맥락 문화 국가의 문자와는 확연히 다른 성질을 지니고 있는데, 내포된 의미의 다양성과 그 의미의 변화가능성 등이 그 특징이다. 한편, 중국의 예술 디자인 분야에서는 한자를 핵심디자인 요소로 활용한 작품들이 늘어나는 추세이다. 이러한 작품들은 한자의 형태, 구조, 의미, 매개 등 서로 다른 측면을 통해 한자의 아름다움을 보여주고 있다. 그 중에서도 한자 의미의 특징을 응용한 작품들은 특히 그 함의가 풍부하다. 그렇다면 한자는 고맥락 언어적 관점에서 어떤 특징을 지니고 있으며, 이러한 특징들은 어떻게 현대 디자인 작품에 적용되고 있는지를 고찰하는 것이 본 연구의 목적이다. 본 연구는 관련 문헌의 수집과 정리를 통해 위에서 제기한 문제에 대한 분석을 진행하였다. 우선 고맥락문화의 개념 및 특징을 정리하고 고맥락 문화 차원에서 한자의 철학과 사회적 요소에 대해 고찰한다. 이어서 한자의 의미가 가지는 심미적 특징을 고맥락 문화의 관점에서 종합적으로 개괄함으로써 한자 의미의 심미적 특징인 ‘함축’, ‘허실’, ‘은유’의 3가지 핵심요소를 도출하였다. 마지막으로 한자의 의미를 주제로 한 시각디자인 작품을 선별하여 한자 의미의 심미적 특징 3요소가 현대 디자인에서 어떻게 활용되고 있는지 분석하였다. 이상의 방법을 통해 본 연구는 한자의 의미를 주제로 하여 그 심미적 특징을 표현하는 디자인 응용에 참고 자료를 제공하고자 한다. China is a typical country with high-context culture, so the way Chinese people express themselves at this level is unique. Chinese characters inherit civilization, record ideas, and serve as an important medium for daily communication. Especially in the semantic sense, it shows a completely different temperament from the countries that use characters in the low-context culture, because the contents contained in Chinese characters are richer, more changeable, and more possible. In various design fields, works with Chinese characters as the core design elements are common. These works show the beauty of Chinese characters through different aspects such as the form, structure, semantics, and media of Chinese characters, among which the semantic features of Chinese characters are especially rich. Then, what are the aesthetic characteristics of Chinese characters in the context of high-context cultural linguistics? In addition, the purpose of this study is to observe how these characteristics are applied to design works. Through collecting and sorting out relevant literature, this paper will analyze and study the above problems. Firstly, the concept and characteristics of contextual culture are summarized, and the philosophical and social factors that form high-context culture are observed from the dimension of high-context culture. Secondly, it generalizes the aesthetic characteristics of Chinese characters from the perspective of high-context culture. By sorting out the relevant literature views of “high-context and Chinese character semantics”, it is concluded that the beauty of Chinese character semantics in high-context culture is mainly reflected in its artistic conception. Besides, it also gets three core elements from the literature. Finally, the visual design works with the meaning of Chinese characters as the theme are selected to observe the application of semantic aesthetic characteristics of Chinese characters in the design. Thus, it provides some reference for the application of Chinese character semantical aesthetics in design.

한자의 심미적 특징에 기초한 한자 디자인 응용 연구 - 근태강(靳埭强) 작품을 연구 대상으로 -

치야쉔 ( Ya Xuan Qi ),장주영 ( Ju-young Chang ) 한국디자인트렌드학회 2019 한국디자인포럼 Vol.24 No.2

연구배경 문자는 문화를 전승하는데 중요한 매개체로서, 한 민족의 내재된 심미적 정취와 사고방식을 담고 있는 민족 문화와 정신이 집중된 표현이다. 한자는 중국에서 고대부터 지금까지 오래 전승된 옛 문자이자, 오늘날 세계에서 널리 사용되고 있는 유일한 표의문자로, 디자인 분야에서 지속적으로 중요시했던 디자인 자원이다. 연구방법 본 논문에서는 형태, 구조, 의미의 세 가지 관점에서 한자의 발전 경로 서술을 시작으로, 한자의 세가지 심미적 특징을 다루고, 근태강(靳埭强) 선생의 디자인 작품에 대한 분석을 통해, 한자의 심미적 특징이 현대 디자인에서 어떻게 응용되고 있는지를 살펴봄으로써, 한자의 아름다움이 오늘날 디자인에서 표현되는 경로와 특징을 탐색하였다. 연구결과 한자의 세 가지 심미적 특징을 통해 한자와 현대적 디자인을 적극적으로 융합한 근태강 선생 작품을 분석하여, 중국적 정취를 공유하고 있는 작품의 경우 위 분석틀이 유의미한 결과물을 도출할 수 있다는 사실을 알게 되었다. 결론 한자의 심미적 특징은 중국 디자인에서 중요한 미학적 임무를 수행하고 있으며, 중국의 현대 디자인 작품을 이해하는 데 유의미한 참조점으로 기능한다. Background As an important carrier of cultural inheritance, characters contain a nation's inherent aesthetic taste and way of thinking, which is the concentrated reflection of a nation's culture and spirit. As an ancient Chinese character, Chinese characters that has been handed down since ancient times are the only ideographic characters widely used in the world. Methods Taking the development path of Chinese characters as the clue, this paper expounds the three aesthetic characteristics of Chinese characters. Through the analysis of the design works of Kan TaiKeung, I observed the application of the aesthetic characteristics of Chinese characters in modern design from the perspectives of form, structure and semantics, so as to explore the expression path of the beauty of Chinese characters in today's design. Result The analysis of Kan TaiKeung’s works with three aesthetic characteristics of Chinese characters, this paper draws an conclustion that the works which is based on Chinese culture can be analyzed successfully with the frame. Conclusion The aesthetic characteristics of Chinese character serves a significant position in Chinese design, and it is a great reference to understand other chinese design works which share chinese cultural background.

Matrine Reduces Proliferation of Human Lung Cancer Cells by Inducing Apoptosis and Changing miRNA Expression Profiles

Liu, Yong-Qi,Li, Yi,Qin, Jie,Wang, Qian,She, Ya-Li,Luo, Ya-Li,He, Jian-Xin,Li, Jing-Ya,Xie, Xiao-Dong Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.5

Matrine, a main active component extracted from dry roots of Sophora flavecens, has been reported to exert antitumor effects on A549 human non-small lung cancer cells, but its mechanisms of action remain unclear. To determine effects of matrine on proliferation of A549 cells and assess possible mechanisms, MTT assays were employed to detect cytotoxicity, along with o flow cytometric analysis of DNA content of nuclei of cells following staining with propidium iodide to analyze cell cycle distribution. Western blotting was performed to determined expression levels of Bax, Bcl-2, VEGF and HDAC1, while a microarray was used to assessed changes of miRNA profiles. In the MTT assay, matrine suppressed growth of human lung cancer cell A549 in a dose- and timedependent manner at doses of 0.25-2.5 mg/ml for 24h, 48h or 72h. Matrine induced cell cycle arrest in G0/G1 phase and decreased the G2/M phase, while down-regulating the expression of Bcl2 protein, leading to a reduction in the Bcl-2/Bax ratio. In addition, matrine down regulated the expression level of VEGF and HDAC1 of A549 cells. Microarray analysis demonstrated that matrine altered the expression level of miRNAs compared with untreated control A549 cells. In conclusion, matrine could inhibit proliferation of A549 cells, providing useful information for understanding anticancer mechanisms.